Clarification of the redox potential regulation in electron transfer reaction of photosystem

• Project/Area Number: 19614007
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 光生命科学
• Research Institution: Kyoto University
• Principal Investigator: TOMO Tatsuya Kyoto University, 大学院・人間環境学研究科, 研究員 (60300886)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2007: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 光合成 / 光化学系 / シアノバクテリア / 酸化還元電位 / クロロフィル / 反応中心 / Acaryochoris / Acaryochloris

Research Abstract

クロロフィルdを主要色素としてもつ、シアノバクテリアAcaryochloris marinaはその吸収帯がクロロフィルaと比較して約100mV低く、酸化還元電位の制御において非常に興味深い生物である。この生物から光化学系I, IIを単離精製し、その反応中心色素がクロロフィルdであることを定常および時間分解分光測定により決定した。また、その酸化還元電位はクロロフィルa型の光化学系と変わらないことを明らかにした。これは、酸化還元電位の調節が還元側で制御されていることを意味しており、光化学系電荷分離の基本的原理を構築するものである。

Research Products

• [Journal Article] Replacement of chlorophyll with di-vinyl chlorophyll in the antenna and reaction center complexes of the cyanobacterium Synechocystis sp. PCC 6803 : Characterization of spectral and photochemical properties (2009)
  • Author(s): Tatsuya Tomo, Seiji Akimoto, Hisashi Ito, Tohru Tsuchiya, Michitaka Fukuya, Ayumi anaka
  • Journal Title: and Mamoru Mimuro Biochimica Biophysica Acta 1787 Pages: 191-200
  • Peer Reviewed
• [Journal Article] Detection of the D_0 → D_1 transition of ss-carotene radical cation photoinduced in photosystem II (2009)
  • Author(s): Tatsunori Okubo, Tatsuya Tomo, Takumi Noguchi
  • Journal Title: Photochemical & Photobiological Science 8 Pages: 157-161
  • Peer Reviewed
• [Journal Article] Replacement of chlorophyll with di-vinyl chlorophyll in the antenna and reaction center complexes of the cyanobacterium Synechocystis sp. PCC 6803 : Characterization of spectral and photochemical properties (2009)
  • Author(s): Tatsuya Tomo, Seiji Akimoto, Hisashi Ito, Tohru Tsuchiya, Michitaka Fukuya, Ayumi Tanaka, Mamoru Mimuro
  • Journal Title: Biochimica Biophysica Acta 1787 Pages: 191-200
  • Peer Reviewed
• [Journal Article] Detection of the D_0→ D_1 transition of β-carotene radical cation photoinduced in photosystem II (2009)
  • Author(s): Tatsunori Okubo, Tatsuya Tomo, Takumi Noguchi
  • Journal Title: Photochemical & Photobiological Science 8 Pages: 157-161
  • Peer Reviewed
• [Journal Article] Characterization of highly purified photosystem I complexes from the chlorophyll d-dominated cyanobacterium Acaryochloris marina, MBIC 11017 (2008)
  • Author(s): Tatsuya Tomo, Yuki Kato, Takehiro Suzuki Seiji Akimoto, Tatsunori Okubo, Takumi Noguchi, Koji Hasegawa, Tohru Tsuchiya, Kazunori Tanaka, Michitaka Fukuya, Naoshi Dohmae, Tadashi Watanabe, and Mamoru Mimuro
  • Journal Title: Journal of Biological Chemistry 283 Pages: 18198-18209
  • Peer Reviewed
• [Journal Article] Isolation and spectral characterization of Photosystem II reaction center from Synechocystis sp. PCC 6803 (2008)
  • Author(s): Tatsuya Tomo, Seiji Akimoto, Tohru Tsuchiya, Michitaka Fukuya, Kazunori Tanaka, and Mamoru Mimuro
  • Journal Title: Photosynthesis Research 98 Pages: 293-302
  • NAID: 130006991250
  • Peer Reviewed
• [Journal Article] Two unique cyanobacterium lead to a traceable approach of the first appearance of oxygenic photosynthesis (2008)
  • Author(s): Mamoru Mimuro, Tatsuya Tomo, Tohru Tsuchiya
  • Journal Title: Photosynthesis Research 97 Pages: 167-176
  • Peer Reviewed
• [Journal Article] Niche adaptation and genome expansion in the chlorophyll d-producing cyanobacterium Acaryochloris marina (2008)
  • Author(s): Wesley D. Swingley, Min Chen, Patricia C. Cheung, Amber L. Conrad, Liza C. Dejesa, Jicheng Hao, Barbara M. Honchak, Lauren E. Karbach, Ahmet Kurdoglu, Surobhi Lahiri, Stephen D. Mastrian, Hideaki Miyashita, Lawrence Page, Pushpa Ramakrishna, Soichirou Satoh, W. Matthew Sattley, Yuichiro Shimada, Heather L. Taylor, Tatsuya Tomo, Tohru Tsuchiya, Zi T. Wang, Jason Raymond, Mamoru Mimuro, Robert E. Blankenship, and Jeffrey W. Touchman
  • Journal Title: Proceedings of the National Academy of Science USA 105 Pages: 2005-2010
  • Peer Reviewed
• [Journal Article] Spectral properties of the CP43-deletion of Synechocystis sp. PCC6803 (2008)
  • Author(s): Yuichiro Shimada, Tohru Tsuchiya, Seiji Akimoto, Tatsuya Tomo, Michitaka Fukuya, Kazunori Tanaka, Mamoru Mimuro
  • Journal Title: Photosynthesis Research 98 Pages: 303-314
  • Peer Reviewed
• [Journal Article] Characterization of highly purified photosystem I complexes from the chlorophyll d-dominated cyanobacterium Acaryochloris marina, MBIC 11017 (2008)
  • Author(s): Tatsuya Tomo, Yuki Kato, Takehiro Suzuki, Seiji Akimoto, Tatsunori Okubo, Takumi Noguchi, Koji Hasegawa, Tohru Tsuchiya, Kazunori Tanaka, Michitaka Fukuya, Naoshi Dohmae, Tadashi Watanabe, Mamoru Mimuro
  • Journal Title: Journal of Biological Chemistry 283 Pages: 18198-18209
  • Peer Reviewed
• [Journal Article] Isolation and spectral characterization of Photosystem II reaction center from Synechocystis sp. PCC 6803 (2008)
  • Author(s): Tatsuya Tomo, Seiji Akimoto, Tohru Tsuchiya, Michitaka Fukuya, Kazunori Tanaka, Mamoru Mimuro
  • Journal Title: Photosynthesis Research 98 Pages: 293-302
  • NAID: 130006991250
  • Peer Reviewed
• [Journal Article] Solvent effects on excitation relaxation dynamics of a keto-carotenoid, siphonaxanthin (2008)
  • Author(s): Seiji Akimoto, Makio Yokono, Michiya Higuchi, Tatsuya Tomo, Sinichi Takaichi, Akio Murakami, Mamoru Mimuro
  • Journal Title: Photochemical & Photobiological Science 1 Pages: 223-226
  • Peer Reviewed
• [Journal Article] Identification of the special pair of photosystem II in a chlorophyll d-dominated cyanobacterium (2007)
  • Author(s): Tatsuya Tomo, Tatsunori Okubo, Seiji Akimoto, Makio Yokono, Hideaki Miyashita, Tohru Tsuchiya, Takumi Noguchi, Mamoru Mimuro
  • Journal Title: Proceedings of the National Academy of Science USA 104 Pages: 7283-7288
  • Peer Reviewed
• [Journal Article] Identifiation of the special pair of photosystem II in a chlorophyll d-dominated cyanobacterium (2007)
  • Author(s): Tatsuya Tomo, Tatsunori Okubo, Seiji Akimoto, Makio Yokono,
  • Journal Title: Proceedings of the National Academy of Science USA 104 Pages: 7283-7288
  • Peer Reviewed
• [Journal Article] Identification of a new excited state responsible for the in vivo unique absorption band of siphonaxanthin in the green alga Codium fragile (2007)
  • Author(s): Seiji Akimoto, Tatsuya Tomo, Yukito Naitoh, Akira Otomo, Akio
  • Journal Title: The Journal of Physical Chemistry B 111 Pages: 9179-9181
  • Peer Reviewed
• [Presentation] Synechocystis sp. PCC 6803からの光化学系II反応中心複合体の単離と分光特性の解析 (2009)
  • Author(s): 鞆達也, 秋本誠志, 土屋徹, 福谷通孝, 田中一徳, 三室守
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋大学東山キャンパス
• [Presentation] Synechocystis sp. PCC 6803からの光化学系II反応中心複合体の単離と分光特性の解析 (2009)
  • Author(s): 鞆達也, 秋本誠志, 土屋徹, 福谷通孝, 田中一徳, 三室守
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋大学
• [Presentation] 光化学系IIの酸素発生系におけるCP43-354位のグルタミン酸残基の役割 (2009)
  • Author(s): 嶋田友一郎, 鈴木博行, 土屋徹, 鞆達也, 野口巧, 三室守
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋大学
• [Presentation] 光化学系IIの酸素発生系におけるCP43-354位のグルタミン酸残基の役割 (2008)
  • Author(s): 嶋田友一郎、鈴木博行、土屋徹、鞆達也、野口巧、三室守
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋大学東山キャンパス
• [Presentation] 分光電気化学的手法によるAcaryochloris marinaの光化学系I一次電子供与体の酸化還元電位計測 (2008)
  • Author(s): 加藤祐樹、鞆達也、仲村亮正、土屋徹、三室守、渡辺正
  • Organizer: 第49回日本植物生理学会年会
  • Place of Presentation: 札幌コンベンションセンター
• [Presentation] クロロフィルdを主要色素としてもつシアノバクテリアの光化学系Iのサブユニット組成と光化学反応の解析 (2008)
  • Author(s): 鞆達也、加藤祐樹、鈴木健裕、秋本誠志、野口巧、土屋徹、堂前直、渡辺正、三室守
  • Organizer: 第49回日本植物生理学会年会
  • Place of Presentation: 札幌コンベンションセンター
• [Presentation] 光化学系II-CP43欠損株における蛍光特性評価 (2008)
  • Author(s): 嶋田友一郎、土屋徹、秋本誠志、田中一徳、福谷通孝、鞆達也、三室守
  • Organizer: 第49回日本植物生理学会年会
  • Place of Presentation: 札幌コンベンションセンター
• [Presentation] (2007)
  • Author(s): Akimoto, M. Yokono, T. Tomo, A. Murakami, and M. Mimuro
  • Organizer: 7^<th> International Conference on Tetrapyrrole Photoreceptors in Photosynthetic Organisms
  • Place of Presentation: Kyoto (Japan)
• [Presentation] Components and photochemical reactions in photosystem II of the Chl d-dominated cyanobacterium, Acaryochloris marina (2007)
  • Author(s): M. Mimuro, T. Tomo, T. Tsuchiya, T. Okubo, T. Noguchi, and S. Akimoto
  • Organizer: 7^<th> International Conference on Tetrapyrrole Photoreceptors in Photosynthetic Organisms
  • Place of Presentation: Kyoto (Japan)
• [Presentation] The characterization of Photosystem I and II in a chlorophyll d-dominated cyanobacterium (2007)
  • Author(s): T. Tomo, Y. Kato, S. Akimoto, T. Noguchi, T. Tsuchiya, T. Watanabe, and M. Mimuro
  • Organizer: 7^<th> International Conference on Tetrapyrrole Photoreceptors in Photosynthetic Organisms
  • Place of Presentation: Kyoto (Japan)
• [Presentation] Identification of special pair and Chl_Z of Photosystem II in Acaryochloris marina (2007)
  • Author(s): T. Tomo, T. Okubo, S. Akimoto, H. Miyashita, T. Tsuchiya, T. Noguchi, and M. Mimuro
  • Organizer: 14th International Congress of Photosynthesis
  • Place of Presentation: Glasgow (UK)
• [Presentation] Unique optical properties of LHC II isolated from Codium fragile - its correlation to protein environment (2007)
  • Author(s): T. Tsuchiya, T. Tomo, S. Akimoto, A. Murakami, and M. Mimuro
  • Organizer: 14th International Congress of Photosynthesis
  • Place of Presentation: Glasgow (UK)
• [Presentation] 溶液中におけるケトカロテノイドの超高速励起緩和 (2007)
  • Author(s): 秋本誠志, 村井陽介, 横野牧生, 村上明男, 高市真一, 樋口倫也, 鞆達也, 三室守
  • Organizer: 第23回化学反応討論会
  • Place of Presentation: 神戸大学百年記念館六甲ホール
• [Presentation] Identification of special pair and Ch1Z of Photosystem II in Acaryochloris marina (2007)
  • Author(s): T. Tomo, T. Okubo, S. Akimoto, H. Miyashita, T. Tsuchiya, T. Noguchi, and M. Mimuro
  • Organizer: 14th International Congress of Photosynthesis
  • Place of Presentation: Glasgow(UK)
• [Presentation] Unique optical properties of LHC II isolated from Codium fragile-its correlation to Protein environment (2007)
  • Author(s): T. Tsuchiya, T. Tomo, S. Akimoto, A. Murakami, and M. Mimuro
  • Organizer: 14th International Congress of Photosynthesis
  • Place of Presentation: Glasgow(UK)
• [Presentation] The characterization of Photosystem I and II in a chlorophylld-dominated cyanobacterium (2007)
  • Author(s): T. Tomo, Y. Kato, S. Akimoto, T. Noguchi, T. Tsuchiya, T. Watanabe, and M. Mimuro
  • Organizer: 7th International Conference on Tetrapyrrole Photoreceptors in Photosynthetic Organisms
  • Place of Presentation: Kyoto TERSA
• [Presentation] Components and photochemical reactions in photosystem II of the Chl d-dominated cyanobacterium,Acaryochloris marina (2007)
  • Author(s): M. Mimuro, T. Tomo, T. Tsuchiya, T. Okubo, T. Noguchi, and S. Akimoto
  • Organizer: 7th International Conference on Tetrapyrrole Photoreceptors in Photosynthetic Organisms
  • Place of Presentation: Kyoto TERSA
• [Presentation] Excited state dynamics and functions of carotenoids in the light-harvesting complex II (2007)
  • Author(s): S. Akimoto, M. Yokono, T. Tomo, A. Murakami, and M. Mimuro
  • Organizer: 7th International Conference on Tetrapyrrole Photoreceptors in Photosynthetic Organisms
  • Place of Presentation: Kyoto TERSA