ヒトABCトランスポーターの遺伝子多型を認識するタンパク品質管理機構の解明

• Project/Area Number: 19659136
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Applied pharmacology
• Research Institution: The Institute of Physical and Chemical Research (2009); Tokyo Institute of Technology (2007-2008)
• Principal Investigator: 石川 智久 The Institute of Physical and Chemical Research, オミックス基盤研究領域, 客員主管研究員 (60193281)
• Co-Investigator(Kenkyū-buntansha): 中川 大 東京工業大学, 大学院・生命理工学研究科, 助教 (40397039)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,200,000 (Direct Cost: ¥3,200,000); Fiscal Year 2009: ¥1,000,000 (Direct Cost: ¥1,000,000); Fiscal Year 2008: ¥1,000,000 (Direct Cost: ¥1,000,000); Fiscal Year 2007: ¥1,200,000 (Direct Cost: ¥1,200,000)
• Keywords: タンパク質 / 糖鎖 / 遺伝子多型 / トランスポーター / 翻訳後修飾

Research Abstract

ヒトABC(ATP-Binding Cassette)トランスポーターは、細胞内代謝物の輸送や癌の抗癌剤耐性に関与する重要な膜タンパク質である。平成21年度我々は、ヒトABCトランスポーターABCC11のSNPの中でも、タンパク質の安定性が変化し発現レベルが変わるSNPに注目し、その多型を認識するタンパク質品質管理機構の細胞内分子メカニズムを解明した。タンパク質の発現レベルが著しく低いABCC11の非同義置換G180R(Gly180Arg)SNPバリアントは、ユビキチン化を経て分解されていることを証明した(Toyoda Y, et al.Earwax, osmidrosis, and breast cancer : why does one SNP(538G>A)in the human ABC transporter ABCC11 gene determine earwax type? FASEB J.23(6):2001-2013.2009)。一方、ABCG2の糖鎖付加は複数のステップを経て進行すると考えられる。それぞれのステップに特異的阻害剤をABCG2発現細胞に作用させ、ABCG2の発現量・発現部位(形質膜・細胞内)・輸送機能等の変化を解明した。その結果、ABCG2蛋白質の安定性において、ジスルフィド結合に加えて、N-結合性糖鎖が重要であることが判った(Nakagawa H, et al.Disruption of N-linked glycosylation enhances ubiquitin-mediated proteasomal degradation of human ATP-binding cassette transporter ABCG2. FEBS J.276(24),7237-7252,2009)。

Research Products

• [Journal Article] Production of cells with targeted integration of gene variants of human ABC transporter for stable and regulated expression using the F1p recombinase system (2010)
  • Author(s): Wakabayashi-Nakao K, Tamura A, Koshiba S, Nakagawa H, Ishikawa T.
  • Journal Title: Methods in Molecular Biology (印刷中)
  • Peer Reviewed
• [Journal Article] Earwax, osmidrosis, and breast cancer why does one SNP(538G>A) in the human ABC transporter ABCC11 gene determine earwax type? (2009)
  • Author(s): Toyoda Y, Sakurai A, Mitani Y, Nakashima M, Yoshiura K, Nakagawa H, Sakai Y, Ota I, Lezhava A, Hayashizaki Y, Niikawa N, Ishikawa T.
  • Journal Title: FASEB Journal 23 Pages: 2001-2013
  • Peer Reviewed
• [Journal Article] Disruption of N-linked glycosylation enhances ubiquitin-mediated proteasomal degradation of human ATP-binding cassette transporter ABCG2 (2009)
  • Author(s): Nakagawa H, Wakabayashi-Nakao K, Tamura A, Toyoda Y, Koshiba S, Ishikawa T.
  • Journal Title: FEBS Journal 276 Pages: 7237-7252
  • Peer Reviewed
• [Journal Article] Quality control of human ABCG2 protein in the endoplasmeic reticulm : ubiquitination and proteasomal degradation (2009)
  • Author(s): Wakabayashi-Nakao K, Tamura A, Furukawa T, Nakagawa H, Ishikawa T.
  • Journal Title: Advances in Drug Delivery Review 61 Pages: 66-72
  • Peer Reviewed
• [Journal Article] Major SNP(Q141K)variant of human ABC transporter ABCG2 undergoes lysosomal and proteosomal degradations (2009)
  • Author(s): Furukawa T, Wakabayashi K, Tamura A, Nakagawa H, Morishima Y, Osawa Y, Ishikawa T.
  • Journal Title: Pharmaceutical Research 26 Pages: 469-479
  • Peer Reviewed
• [Journal Article] 個別化医療」の市場とわが国における課題克服 (2009)
  • Author(s): 石川智久
  • Journal Title: MedicalBio 別冊(7月) Pages: 24-31
• [Journal Article] 薬物トランスポーターのSNP診断 (2009)
  • Author(s): 石川智久
  • Journal Title: MedicalBio 別冊(7月) Pages: 78-83
• [Journal Article] A rapid and cost-effective SNP detection method : Application of SmartAmp2 to pharmacogenomics research (2009)
  • Author(s): Mitani Y, Lezhava A, Sakurai A, Horikawa A, Nagakura M, Hayashizaki Y, Ishikawa T.
  • Journal Title: Pharmacogenomics (印刷中)
  • Peer Reviewed
• [Journal Article] Production of cells with targeted integration of gene variants of human ABC transporter for stable and regulated expression using the Flprecombinase system (2009)
  • Author(s): Wakabayashi-Nakao K, Tamura A, Koshiba H, Nakagawa H, Ishikawa T.
  • Journal Title: Methods Mol. Biol. (印刷中)
  • Peer Reviewed
• [Journal Article] Major SNP (Q141K)variant of human ABC transporter ABCG2 Undergoes lysosomal and proteosomal degradations (2009)
  • Author(s): Furukawa T, Wakabayashi K, Tamura A, Nakagawa H, Morishima Y, Osawa Y, Ishikawa T
  • Journal Title: Pharmaceutical Research 26(2) Pages: 469-479
  • Peer Reviewed
• [Journal Article] Emerging Trends in Human ABC Transporters (2009)
  • Author(s): Ishikawa T.
  • Journal Title: Pharmaceutical Research 26(2) Pages: 445-448
  • Peer Reviewed
• [Journal Article] Quality control of human ABCG2 protein in the endoplasmic reticulm : ubiquitination and proteasomal degradation (2009)
  • Author(s): Wakabayashi K, Tamura A, Furukawa T, Nakagawa H, Ishikawa T.
  • Journal Title: Advanced Drug Delivery Reviews 61 Pages: 66-72
  • Peer Reviewed
• [Journal Article] Human ABC transporters ABCG2 (BCRP)and ABCG4 (2008)
  • Author(s): Koshiba S, An R, Saito H, Wakabayashi K, Tamura A, Ishikawa T.
  • Journal Title: Xenobiotica 38(7-8) Pages: 863-888
  • Peer Reviewed
• [Journal Article] ポルフィリンの光励起によるヒトABCトランスポーターABCG2とヘムオキシゲナーゼの誘導におけるNrf2の役割 (2008)
  • Author(s): 萩谷祐一郎, 足立達彦, 小倉俊一郎, 安然, 田村藍, 中川大, 望月徹, 石川智久
  • Journal Title: Porphyrins (印刷中)
  • Peer Reviewed
• [Journal Article] Unibiquitin-mediated proteasomal degradation of nonsynonymous SNP variants of human ABC transporter ABCG2. (2008)
  • Author(s): Nakagawa H, Tamura A, Wakabayashi K, Hoshijima K, Komada M, Yoshida T, Kometani S, Matsubara T, Mikuriya K, 'Ishikawa T.
  • Journal Title: Biochem. J. 411 Pages: 623-631
  • Peer Reviewed
• [Journal Article] Cellular phototoxicity evoked through the inhibition of human ABC transporter ABCG2 by cyclin-dependent kinase inhibitors in vitro. (2008)
  • Author(s): An R, Hagiya Y, Tamura A, Li S, Saito H, Tokushima D, and Ishikawa T.
  • Journal Title: Photochem. Photobiol. Sci (in press)
  • Peer Reviewed
• [Journal Article] Drug-induced phototoxicity evoked by inhibition of human ABC transporter ABCG2: Development of in vitro high-speed screening systems. (2008)
  • Author(s): Tamura A, An R, Hagiya Y, Hoshijima K, Yoshida T, Mikuriya K, and Ishikawa T.
  • Journal Title: Expert Opinion on Drug Metabolism and Toxicology 4(3) Pages: 255-272
  • Peer Reviewed
• [Journal Article] MRP class of human ABC transporters: Role of glutathione in the function and gene regulation. (2008)
  • Author(s): Toyoda Y, Hagiya Y, Adachi T, Hoshijima K, Kuo MT, Ishikawa T.
  • Journal Title: Xenobiotica (in press)
  • Peer Reviewed
• [Journal Article] Human ABC transporters ABCG2 (BCRP) and ABCG4. (2008)
  • Author(s): Koshiba S, An R, Saito H, Wakabayashi K, Tamura A, and Ishikawa T.
  • Journal Title: Xenobiotica (in press)
  • Peer Reviewed
• [Journal Article] Intramolecular disulfide bond is a critical checkpoint determining degradative fates of ABC transporter ABCG2 protein. (2007)
  • Author(s): Wakabayashi K, Nakagawa H, Tamura A, Koshiba S, Hoshijima K, Komada M, and Ishikawa T.
  • Journal Title: J. Biol. Chem. 282(38) Pages: 27841-27846
  • Peer Reviewed
• [Journal Article] In vitro evaluation of photosensitivity risk related to genetic polymorphisms of human ABC transporter ABCG2 and inhition by drugs. (2007)
  • Author(s): Tamura A, Onishi Y, An R, Koshiba S, Wakabayashi K, Hoshijima K, Priebe W, Yoshida T, Kometani, S, Matsubara T, Mikuriya K, and Ishikawa, T.
  • Journal Title: Drug Metab. Pharmacokin. 22(6) Pages: 428-440
  • Peer Reviewed
• [Journal Article] Nrf2-dependent and -independent induction of ABC transporters ABCC1, ABCC2, and ABCG2 in HepG2 cells under oxidative strees. (2007)
  • Author(s): Adachi T, Nakagawa H, Chung I, Hagiya Y, Hoshijima K, Noguchi N, Kuo MT and Ishikawa, T.
  • Journal Title: J. Exp. Ther. Oncol. 6 Pages: 335-348
  • Peer Reviewed
• [Journal Article] Development of polyclonal antibodies specific to ATP-binding casstte transporters human ABCG4 and mouse Abcg4: site-specific expression of mouse Abcg4 in brain. (2007)
  • Author(s): Koshiba S, Ito T, Shiota A, Wakabayashi K, Ueda M, Ichinose H, and Ishikawa T.
  • Journal Title: J. Exp. Ther. Oncol. 6 Pages: 321-333
  • Peer Reviewed
• [Journal Article] High-speed screening and quantitative SAR analysis of human ABC transporter ABCG2 for molecular modeling of anticancer drugs to circumvent multidrug resistance. (2007)
  • Author(s): Saito H, Hirano H and Ishikawa T.
  • Journal Title: Mini Rev. Medcin. Chem. 7 Pages: 1009-1018
  • Peer Reviewed
• [Journal Article] Multidrug resistance-associated protein 9 (ABCC12) is present in sperm. (2007)
  • Author(s): Ono N, van der Heiden I, Schffer GL, van de Wetering, van Deenter E, de Haas M, Boerke A, Gadella BM, de Rooij DG, Neefjes JJ, Groothuis TAM, Oomen L, Brocks L, Ishikawa T, and Borst P.
  • Journal Title: Biochem. J. 406(1) Pages: 31-40
  • Peer Reviewed
• [Journal Article] Quantitative SAR analysis and molecular dynamic simulation to functionally validate nonsynonymous polymorphisms of human ABC transporter ABCB1. (2007)
  • Author(s): Sakurai A, Onishi Y, Hirano H, Seigneuret M, Obanayama K, Kim G, Liew EL, Sakaeda T, Yoshiura K, Niikawa N, Sakurai M and Ishikawa T.
  • Journal Title: Biochemistry 46(26) Pages: 7678-7693
  • Peer Reviewed
• [Journal Article] Re-evaluation and functional classification of nonsynonymous single nucleotide polymoiphisms of human ABC transporter ABCG2. (2007)
  • Author(s): Tamura A, Wakabayashi K, Onishi Y, Takeda M, Ikegami Y, Sawada S, Tsuji M, Matsuda Y and Ishikawa T.
  • Journal Title: Cancer Sci. 98(2) Pages: 231-239
  • Peer Reviewed
• [Journal Article] Prediction of drug-induced intrahepatic cholestasis: in vitro screening and QSAR analysis of drugs inhibiting the human bile salt export pump. (2007)
  • Author(s): Sakurai A, Kurata A, Onishi Y, Hirano H and Ishikawa T.
  • Journal Title: Expert Opin. Drug Saf. 6(1) Pages: 71-86
  • Peer Reviewed
• [Journal Article] Identification of two biologically crucial hydroxyl groups of(-)-epigallocatechin gallate in osteoclast culture. (2007)
  • Author(s): Nakagawa H, Hasumi K, Takami M, Aida-Hyugaji S, Woo JT, Nagai K, Ishikawa T and Wachi M.
  • Journal Title: Biochem. Pharmacol. 73(1) Pages: 34-43
  • Peer Reviewed
• [Journal Article] Theoretical studies for molecular modeling of new camptothecin analogues. (2007)
  • Author(s): Aida-Hyugaji S, Nakagawa H, Nomura J, Sakurai M, Nagashima U and Ishikawa T.
  • Journal Title: Croatica Chemica Acta 80(2) Pages: 277-281
  • Peer Reviewed
• [Journal Article] Association of cumulative cyclosporine dose with its irreversible nephrotoxicity in Japanese patients with pediatric-onset autoimmune diseases. (2007)
  • Author(s): Nakamura T, Nozu K, Iijima K, Yoshikawa N, Moriya Y, Yamamori M, Kako A, Matsuo M, Sakurai A, Okamura N, Ishikawa T, Okumura K, Sakaeda T.
  • Journal Title: Biol. Pharm. Bull. 30(12) Pages: 2371-2375
  • NAID: 110006546441
  • Peer Reviewed
• [Presentation] Genetic Polymorphisms of ABC Transporters in Cancer Chemotherapy : Development of SmartAmp2 Method for Clinical Genetic Testing (2010)
  • Author(s): Ishikawa, T., Aw, W., Ledhava, A., Hayashizaki, Y.
  • Organizer: AACR Conference on ranslational Cancer Medicine 2010(Europe)
  • Place of Presentation: Amsterdam、オランダ
  • Year and Date: 2010-03-23
• [Presentation] Pharmacogenomics of human ABC transporters : from functional validation to clinical application (2010)
  • Author(s): Ishikawa, T., Aw, W., Saito, H., Toyoda, Y., Nakagawa, H., Lezhava, A., Hayashizaki, Y.
  • Organizer: 3^<rd> FEBS Special Meeting on ABC Proteins
  • Place of Presentation: Innsbruck, Austria
  • Year and Date: 2010-03-04
• [Presentation] Earwax, osmidrosis, and breast cancer : why does one SNP(538G>A)in the human ABC transporter ABCC11 gene determine earwax type? (2010)
  • Author(s): Toyoda, Y., Nakagawa, H., Aw, W., Lezhava, A., Hayashizaki, Y., Niikawa, N., Ishikawa, T.
  • Organizer: 3^<rd> FEBS Special Meeting on ABC Proteins
  • Place of Presentation: Innsbruck, Austria
  • Year and Date: 2010-03-04
• [Presentation] Genetic polymorphism in human ABC transporter ABCC11 gene : Potential association between earwax and breast cancer risk (2009)
  • Author(s): Toyoda, Y., Ota, I., Ishikawa, T., Lezhava, A., Hayashizaki, Y., Ishikawa, T.
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] Monomeric non-glycosylated form of human ATP-binding cassette transporter ABCG2 is the most fragile in culture cells (2009)
  • Author(s): Nakagawa, H., Wakabayashi-Nakao, K., Tamura, A., Koshiba, S., Toyoda, Y., Ishikawa, T.
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] Pharmacogenomics of human ABC transporters : From functional SNP analysis to application of SmartAmp2 method in clinical research (2009)
  • Author(s): Ishikawa, T., Lezhava, A., Hayashizaki, Y.
  • Organizer: 24^<th> JSSX Annual Meeting
  • Place of Presentation: 京都
  • Year and Date: 2009-11-25
• [Presentation] Association between the wild type allele in human ABC transporter ABCC11 gene and breast cancer risk : Evaluation by the SmartAmp2 method (2009)
  • Author(s): Ishikawa, T., Toyoda Y, Ota, I., Lezhava, A., Ishikawa, T., Hayashizaki, Y.
  • Organizer: AACR Special Conference in Breast Cancer Research
  • Place of Presentation: San Diego, USA
  • Year and Date: 2009-10-15
• [Presentation] N-linked glycosylation stabilizes the de novo synthesized human ABC transporter ABCG2 protein (2009)
  • Author(s): Nakagawa, H., Wakabayashi-Nakao, K., Tamura, A., Ishikawa, T.
  • Organizer: 日本癌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2009-10-03
• [Presentation] SNP(G538A)of the human ABC transporter ABCC11 correlates with earwax type and breast cancer risk (2009)
  • Author(s): Ishikawa, T., Nakagawa, H., Toyoda, Y., Saito, H., Hayashizaki, Y.
  • Organizer: 日本癌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2009-10-01
• [Presentation] 薬物トランスポーターの遺伝子多型の機能解析:国際標準化からFDA白書まで (2009)
  • Author(s): 石川智久
  • Organizer: イノベーションジャパン
  • Place of Presentation: 東京
  • Year and Date: 2009-09-16
• [Presentation] Human ABC transporter ABCC11 : genetic polymorphisms and molecular mechanisms determining the type of earwax (2009)
  • Author(s): Ishikawa, T., Toyoda, Y., Yoshiura, K., Niikawa, N.
  • Organizer: The 5^<th> Takeda Science Foundation Symposium on PharmaSciences
  • Place of Presentation: 東京
  • Year and Date: 2009-05-26
• [Presentation] Biochemical characterization of human ABC transporter ABCC11 : Its genetic polymorphism determining earwax type (2009)
  • Author(s): Toyoda, Y., Nakagawa, H., Nakashima, M., Yoshiura, K., Niikawa, N., Ishikawa, T.
  • Organizer: The 5^<th> Takeda Science Foundation Symposium on PharmaSciences
  • Place of Presentation: 東京
  • Year and Date: 2009-05-25
• [Presentation] Impact of Major Non-synonymous SNP(Q141K)on Proteolysis of Human ABC Transporter ABCG2 via Lysosomal and Ubiquitin-mediated Proteasomal Degradation Pathways (2009)
  • Author(s): Furukawa, T., Wakabayashi-Nakao, K., Tamura, A., Nakagawa, H., Ishikawa, T.
  • Organizer: The 50^<th> Takeda Science Foundation Symposium on PharmaSciences
  • Place of Presentation: 東京
  • Year and Date: 2009-05-25
• [Presentation] FDA Critical Path Transporter Workshop の報告 (2009)
  • Author(s): 石川智久
  • Organizer: 第23回日本薬物動態学会ワークショップ
  • Place of Presentation: 東京
  • Year and Date: 2009-04-24
• [Presentation] Disruption of N-linked glycosylation enhances ubiquitin-mediated proteasomal degradation of human ATP-binding cassette transporter ABCG2. (2008)
  • Author(s): Nakagawa H, Wakabayashi K, Tamura A, Koshiba S, Toyoda Y, Ishikawa T.
  • Organizer: 日本分子生物学会・日本生化学会合同大会(BMB2008)
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] Biochemical characterization of a genetic polymorphism of human ABCC11 as a determinant of earwax type (2008)
  • Author(s): Toyoda Y, Sakurai A, Nakashima M, Nakagawa H, Yoshiura K, Niikawa N, Ishikawa T.
  • Organizer: 日本分子生物学会・日本生化学会合同大会(BMB2008)
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] Impact of Major Non-synonymous SNP (Q141K)on Proteolysis of Human ABC Transporter ABCG2 via Lysosomal and Ubiquitin-midiated Proteasomal Degradation Pathways (2008)
  • Author(s): Furukawa T, Wakabayashi K, . Tamura A, Nakagawa H, Ishikawa T.
  • Organizer: 日本分子生物学会・日本生化学会合同大会(BMB2008)
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] ヒトABCトランスポーターABCG2の遺伝子多型とERAD : ユビキチン化とプロテアゾーム分解 (2008)
  • Author(s): 若林香菜子, 田村藍, 古川朋佳, 中川大, 石川智久
  • Organizer: トランスポーター研究会第2回関東支部会
  • Place of Presentation: 東京
  • Year and Date: 2008-12-06
• [Presentation] Why does one SNP in the human ABCC11 gene determine earwax type in Homo Sapiens? (2008)
  • Author(s): Toyoda Y, Sakurai A, Nakashima M, Yoshiura K, Nakagawa H, Niikawa N, Ishikawa T.
  • Organizer: トランスポーター研究会第2回関東支部会
  • Place of Presentation: 東京
  • Year and Date: 2008-12-06
• [Presentation] ヒトの耳垢タイプはどのようにして決まるのか? ABCC11の一塩基多型とプロテアソーム分解 (2008)
  • Author(s): 豊田優, 櫻井亜季, 中川大, 吉浦孝一郎, 新川詔夫, 石川智久
  • Organizer: 2008「タンパク質の社会」班会議
  • Place of Presentation: 沖縄
  • Year and Date: 2008-11-25
• [Presentation] Biochemical characterization of a genetic polymorphism of human ABCC11 as a determinant of earwax type. (2008)
  • Author(s): Toyoda Y, Sakurai A, Nakashima M, Nakagawa H, Yoshiura K, Niikawa N, Ishikawa T.
  • Organizer: 2008 桐蔭国際シンポジウム
  • Place of Presentation: 横浜
  • Year and Date: 2008-10-31
• [Presentation] Biochemical characterization of a genetic polymorphism of human ABCC11 as a determinant of earwax type. (2008)
  • Author(s): Toyoda Y, Sakurai A, Nakashima M, Nakagawa H, Yoshiura K, Niikawa N, Ishikawa T.
  • Organizer: 人類遺伝学会
  • Place of Presentation: 横浜
  • Year and Date: 2008-09-29
• [Presentation] Impact of major non-synonymous SNP(Q141K)on proteolysis of ABCG2 via lysosomal and proteasomal degradation pathways (2008)
  • Author(s): Tamura A, Wakabayashi K, An R, Furukawa T, Ishikawa T.
  • Organizer: 人類遺伝学会
  • Place of Presentation: 横浜
  • Year and Date: 2008-09-29
• [Presentation] Genetic Polymorphisms of ABCG2 and ER-associated Degradation (ERAD) Pathway (2008)
  • Author(s): Wakabayashi K, Tamura A, An R, Hoshijima K, Nakagawa H, Ishikawa T.
  • Organizer: 2nd FEBS Special Meeting ABC2008
  • Place of Presentation: Innsbruck, Austria
• [Presentation] Quantitative structure-activity relationship analysis and molecular dynamics simulation to functionally validate nonsynonymous polymorphisms of human ABCG1 (P-glycoprotein/MDR1) (2008)
  • Author(s): Sakurai A, Onishi Y, Liew Ei Leen, Hirano H, Sakurai M, Seigneuret M, Ishikawa T.
  • Organizer: 2nd FEBS Special Meeting ABC2008
  • Place of Presentation: Innsbruck, Austria
• [Presentation] Development of Polyclonal antibodies specific to human ABCG4 and mouse Abcg4: site-specific expression of mouse Abcg4 in brain (2008)
  • Author(s): Koshiba S, Ito T, Wakabayashi K, Ichinose H, Ishikawa T.
  • Organizer: 2nd FEBS Special Meeting ABC2008
  • Place of Presentation: Innsbruck, Austria
• [Presentation] Identification and Inhibitory Characterization of Protein Kinase Inhibitors Based on QSAR Analysis of Human ABC Transporter ABCG2 (2008)
  • Author(s): Saito H, Hirano H, Hirono S, Tokushima D, Ishikawa T.z
  • Organizer: 2nd FEBS Special Meeting ABC2008
  • Place of Presentation: Innsbruck, Austria
• [Presentation] Functional Validate Nonsynonymous Polymorphisms of Human ABCB1 Transporter ABCB1 (P-glycoprotein/MDR1) (2007)
  • Author(s): Sakurai A, Onishi Y, Hirano H, Liew Ei Leen, Obanayama K, Kim G, Sakurai M Ishikawa T
  • Organizer: BMB2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14
• [Presentation] Disulfide Bond Formation Plays a Crucial Role in Protein Stability of Human ABCG2 (2007)
  • Author(s): Wakabayashi K, Nakagawa H, Tamura A, Hoshijima K, Komada M, Ishikawa T
  • Organizer: BMB2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14
• [Presentation] Evidence for ubiquitinqtion and degradation of non-synonymous SNP proteins of human ATP-binding cassette transporter ABCG2 (2007)
  • Author(s): Nakagawa H, Tamura A, Wakabayashi K, Ishikawa T
  • Organizer: BMB2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14
• [Presentation] Transport Mechanism-Based Drug Molecular Design: High-Speed Screening and Quantitative SAR Analysis of ABC Transport ABCG2 to design Novel Anticancer Drugs (2007)
  • Author(s): Saito H, Hirano H, Ikegami Y, Ishikawa T.
  • Organizer: BMB2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] Evaluation and Functional Classification of Nonsynonymous Single Nucleotide Polymorphisms of Human ABC Transporter ABCG2 (2007)
  • Author(s): Tamura A, Wakabayashi K, Ikegami Y, Ishikawa T
  • Organizer: BMB2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] Critical Role of Human ABC Transporter ABCG2 in Cancer Photodynamic Therapy and Porphyria Risk (2007)
  • Author(s): An R, Tamura A, Onishi Y, Ishiikawa T
  • Organizer: BMB2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] Development of polyclonal antibodies specific to ATP-binding cassette transporters human ABCG4 and mouse Abcg4: Site-specific expression of mouse Abcg4 in brain (2007)
  • Author(s): Koshiba S, Ito T, Wakabayashi K, Ichinose H, Ishikawa T.
  • Organizer: BMB2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] Liver X receptor ligands include the expression of ATP-binding cassette transporter ABCG2 in human Hepatoma HepG2 cells (2007)
  • Author(s): Adachi T, Nakagawa H, Hoshijima K, Ishikawa T.
  • Organizer: BMB2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] Development of Expression system of A Novel Human ATP-Binding Cassette treansporter ABCC11 (2007)
  • Author(s): Toyoda Y, Sakurai A, Yoshiura K, Miwa N, Niikawa N, Ishikawa T.
  • Organizer: BMB2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] Nrf2-dependent Induction of ABCC2 and ABCG2 in HepG2 cells under Oxidative Stress (2007)
  • Author(s): Hagiya Y, Adachi T, Nakagawa H, Hoshijima K, Noguchi N, Ishikawa T.
  • Organizer: BMB2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] Characterization and Classification of CDK Inhibitors by QSAR Analysis of Human ABC Transporter ABCG2 (2007)
  • Author(s): Saito H, Hirano H, Hirono S, Tokushima D, Ishikawa T
  • Organizer: 8th International ISSX Meeting
  • Place of Presentation: 仙台
  • Year and Date: 2007-10-10
• [Presentation] QSAR Analysis of Human ABC Transporter ABCB1 (P-GP/MDR1): Effect of SNPs on the Substrate Specificity toward CDK Inhibitors (2007)
  • Author(s): Liew Ei Leen, Sakurai A, Hirano H, Seigneuret M, Ishikawa T
  • Organizer: 8th International ISSX Meeting
  • Place of Presentation: 仙台
  • Year and Date: 2007-10-10
• [Presentation] International Standardization of the Methods for In-vitro Funtional Analysis of Drug Transporters (2007)
  • Author(s): Ishikawa T
  • Organizer: 8th International ISSX Meeting (Short Course2)
  • Place of Presentation: 仙台
  • Year and Date: 2007-10-09
• [Presentation] Critical role of human ABC transporter ABCG2 in cancer photodynamic therapy and porphyria risk (2007)
  • Author(s): Ishikawa T, Wakabayashi K, Saito H, Sakurai A, Tamara A
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2007-10-04
• [Presentation] Disulfide Bond Formation Plays a Crucial Role in Protein Stability of Human ABCG2 (2007)
  • Author(s): Ishikawa T, Wakabayashi K, Saito H, Sakurai A, Tamara A
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2007-10-04
• [Presentation] Evaluation and Functional Classification of Nonsynonymous Single Nucleotide Polymorphisms of Human ABC Transporter ABCG2 (2007)
  • Author(s): Tamura A, Wakabayashi K, Ikegami Y, Ishikawa T
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2007-10-04
• [Presentation] Functionally Validate Nonsynonymous Polymorphisms of Human ABC Transporter ABCB1 (P-glycoprotein/MDR1) (2007)
  • Author(s): Sakurai A, Tamura A, Saito H, Wakabayashi K, Sakaeda T, Ishikawa T
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2007-10-04
• [Presentation] Expression of ABC transporter mRNA in Cololectal cancer Specimen (2007)
  • Author(s): Satake K, Ikegami Y, Sano K, Yamane Y, Ishikawa T
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2007-10-04
• [Presentation] High-Speed Screening and QSAR Analysis of Human ABC Transporter ABCG2 for Molecular Modeling of Anticancer Drugs (2007)
  • Author(s): Saito H, Tamura A, Wakabayashi K, Sakurai A, Ishikawa T
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2007-10-04
• [Presentation] A New Strategy of High-Speed Screening and Quantitative SAR Analysis to Evaluate Human ABC Transporter ABCG2-Drug Interactions (2007)
  • Author(s): Saito H, Hirano H, Osumi M, , Ishikawa T
  • Organizer: Gordon Research Conference on Drug Metabolism
  • Place of Presentation: NH U.S.A.
  • Year and Date: 2007-07-11
• [Presentation] Functional validation of genetic plymorphisms of ABCB1(P-glycoprotein/MDR1): Quantative SAR analysis and moleculae dynamic simulation (2007)
  • Author(s): Sakurai A, Onishi Y, Liew Ei Leen, Hirano H, Sakurai M, Seigneuret M, Ishikawa T,
  • Organizer: Gordon Research Conference on Drug Metabolism
  • Place of Presentation: NH U.S.A.
  • Year and Date: 2007-07-11
• [Presentation] Re-evalution and functional classification of nonsynonymous single nucleotide polymorphisms of human ABC transporter ABCG2 (2007)
  • Author(s): Tamura A, Wakabayashi K, Takeda M, Ikegami Y, Ishikawa T
  • Organizer: 4th World Conference on Drug Absorption, Transport and Delivery
  • Place of Presentation: 金沢
  • Year and Date: 2007-06-21
• [Presentation] Functional validation of genetic polymorphisms of ABCB1 (P-glycoprotein/MDR1): The funtional impact of tetra-allelic SNPs 2677G>T, A, or C (2007)
  • Author(s): Sakurai A, Onishi Y, Liew Ei Leen, Hirano H, Sakurai M, Seigneuret M, Ishikawa T,
  • Organizer: 4th World Conference on Drug Absorption, Transport and Delivery
  • Place of Presentation: 金沢
  • Year and Date: 2007-06-21
• [Presentation] Nrf2/Keap1系によって発現制御を受けるABCトランスポーターの探索 (2007)
  • Author(s): Hagiya H, et. al.
  • Organizer: 第2回トランスポーター研究会
  • Place of Presentation: 東京
  • Year and Date: 2007-06-09
• [Presentation] ヒトABCトランスポーターABCG2のポルフィリン輸送における薬理学的役割:薬物によるABCG2の阻害と光毒性との関係 (2007)
  • Author(s): Adachi T, Nakagawa H, Chung I, Kuo M.T, Ishikawa T.
  • Organizer: 第34回ポルフィリン研究会
  • Place of Presentation: 東京
  • Year and Date: 2007-05-12
• [Presentation] Development of specific antibodies against mouse ATP-binding cassette transporter Abcg4 and expression of Abcg4 in Sf9 insectcells. (2007)
  • Author(s): Koshiba S, Ito T, Ichinose H., Ishikawa, T.
  • Organizer: AACR Annual Meeting 2007
  • Place of Presentation: Los Angeles, U.S.A
  • Year and Date: 2007-04-16
• [Presentation] Nrf2-dependent and indepenndent transcriptional upregulation of ABCC1, ABCC2, ABCC3, and ABCG2 genes in HepG2 cells. (2007)
  • Author(s): Adachi T, Nakagawa H, Chung I, Kuo M. T, Ishikawa T.
  • Organizer: AACR Annual Meeting 2007
  • Place of Presentation: Los Angeles, U.S.A
  • Year and Date: 2007-04-16
• [Presentation] ヒトABCトランスポーターABCG2のポルフィリン輸送における薬理学的役割:薬物によるABCG2の阻害と光毒性との関係 (2007)
  • Author(s): Adachi T, Nakagawa H, Chung I, Kuo M.T, Ishikawa T.
  • Organizer: 第34回ポルフィリン研究会
  • Place of Presentation: Los Angeles, U.S.A.
  • Year and Date: 2007-04-16
• [Book] 小児科診療 (2009)
  • Author(s): 石川智久, 中村亮太, 櫻井亜季
  • Publisher: 株式会社診断と治療社
• [Book] バイオ医薬の開発技術とシーズ (2009)
  • Author(s): 斉藤光, 石川智久
  • Publisher: シーエムシー出版
• [Book] トランスポーター研究最前線 (2008)
  • Author(s): 田村藍, 若林香菜子, 齊藤光, 石川智久
  • Publisher: 廣川書店
• [Book] トランスポーター科学最前線 (2008)
  • Author(s): 辻 彰, 田村 藍, 若林 香奈子, 齊藤 光, 石川 智久, 他28名
  • Total Pages: 374
  • Publisher: 京都 廣川書店
• [Remarks]
  • URL: http://www.ishikawa-lab.bil.titech.ac.jp
• [Remarks]
  • URL: http://www.humanABC.bio.titech.ac.jp
• [Remarks]
  • URL: http://www.ishikawa-lab.bio.titech.ac.jp
• [Remarks]
  • URL: http://www.humanABC.bio.titech.ac.jp