Roles of orphan transporters in multidrug-resistant bacteria and development of therapeutic strategies to control infectious diseases

• Project/Area Number: 19679002
• Research Category: Grant-in-Aid for Young Scientists (S)
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: Osaka University
• Principal Investigator: NISHINO Kunihiko Osaka University, 産業科学研究所, 准教授 (30432438)
• Project Period (FY): 2007 – 2010
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥64,480,000 (Direct Cost: ¥49,600,000、Indirect Cost: ¥14,880,000); Fiscal Year 2011: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000); Fiscal Year 2010: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000); Fiscal Year 2009: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000); Fiscal Year 2008: ¥12,740,000 (Direct Cost: ¥9,800,000、Indirect Cost: ¥2,940,000); Fiscal Year 2007: ¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
• Keywords: 感染症 / 薬剤耐性 / 多剤耐性 / 病原性 / 異物排出 / 薬剤排出 / トランスポーター / 細菌 / 分子生物学 / 輸送体

Research Abstract

現在、臨床現場において様々な多剤耐性菌が出現し、耐性菌感染症は医療従事者が直面する重要な問題である。トランスポーターは生体異物や複数の抗菌薬を細胞外へ排出することにより細菌に多剤耐性をもたらす。これまで、病原細菌に数十個もの薬物・毒物を排出する輸送体が存在することを明らかにした。さらには、サルモネラが宿主中で毒性を発揮するためにトランスポーターの存在が必須であることも見出した。このことは、これらトランスポーターが何らかの生理的基質の輸送体であることを物語っている。これら輸送体は生理的基質が未知という点で、「オーファン輸送体」であると考えられる。本研究では、「オーファン輸送体」が担う生理機能を明らかにし、また、輸送体を阻害することにより、細菌の薬剤耐性と病原性を同時に軽減させることのできる新薬を開発することができるか、可能性を探る。今年度はオーファン輸送体の制御機構を中心に研究を進め、以下の結果を得た。
宿主環境中での細菌オーファン輸送体制御機構を進める中で、腸内細菌が産生するインドールや、宿主が産生する胆汁酸によって、オーファン輸送体が誘導されるという新機構を明らかにした。本誘導には、サルモネラ特異的制御因子が関与していることを明らかにした、さらに、その詳細を明らかにする上では、蛋白質結晶の構造解析が必要であり、誘導シグナルと制御因子の複合体共結晶化に取り組んだ結果、基質結合型制御蛋白質の結晶構造を解くことに成功した。複数の化合物をマルチサイト結合により、制御因子が認識して、オーファン輸送体を誘導していることが明らかになった。
これらの情報をもとに、オーファン輸送体のさらなる機能解明につなげ、耐性菌感染症克服に役立てたい。

Research Products

• [Journal Article] Evaluation of multidrug efflux pump inhibitors by a new method using microfluidic channels (2011)
  • Author(s): Matsumoto, Y., et al.
  • Journal Title: PLoS One Volume: (in press)
  • Peer Reviewed
• [Journal Article] Regulation of the AcrAB multidrug efflux pump in Salmonella enterica serover Typhimurium in response to indole and paraquat (2011)
  • Author(s): Nikaido, E., et al.
  • Journal Title: Microbiology Volume: 157 Pages: 648-655
  • Peer Reviewed
• [Journal Article] Roles of Salmonella multidrug efflux pumps in tigecycline resistance (2011)
  • Author(s): Horiyama, T., et al.
  • Journal Title: J.Antimicrob.Chemother. Volume: 66 Pages: 105-110
  • Peer Reviewed
• [Journal Article] 薬剤排出ポンプによる細菌多剤耐性化・病原性発現制御 (2011)
  • Author(s): 西野邦彦
  • Journal Title: 化学療法の領域 Volume: (in press)
• [Journal Article] Indole enhances acid resistance in Escherichia coli (2010)
  • Author(s): Hirakawa, H., et al.
  • Journal Title: Mtcrob.Pathog. Volume: 49 Pages: 90-94
  • Peer Reviewed
• [Journal Article] Effect of NlpE overproduction on multidrug resistance in Escherichia coli (2010)
  • Author(s): Nishino K., Yamasaki S., Hayashi-Nishino M., Yamaguchi A.
  • Journal Title: Antimicrob. Agents Chemother (in press)
  • Peer Reviewed
• [Journal Article] Impact of RNA chaperone Hfq on multidrug resistance in Escherichia coli (2010)
  • Author(s): Yamada J., Yamasaki S., Hirakawa H., Hayashi-Nishino M., Yamaguchi A., Nishino K.
  • Journal Title: J. Antimicrob. Chemother 65 Pages: 853-858
  • Peer Reviewed
• [Journal Article] Impact of RNA chaperone Hfq on multidrug resistance in Escherichia coli. (2010)
  • Author(s): Yamada, J., et al.
  • Journal Title: J.Antimicrob. Chemother. (In press)
  • Peer Reviewed
• [Journal Article] Effect of NIpE overproduction on multidrug resistance in Escherichia coli. (2010)
  • Author(s): Nishino, K., et al.
  • Journal Title: Antimicrob. Agents Chemother. (In press)
  • Peer Reviewed
• [Journal Article] H-NS modulates multidrug resistance of Salmonella enterica serovar Typhimurium by repressing acrEF multidrug efflux genes (2009)
  • Author(s): Nishino K., Hayashi-Nishino M., Yamaguchi A.
  • Journal Title: Antimicrob. Agents Chemother 53 Pages: 3541-3543
  • Peer Reviewed
• [Journal Article] Regulation and physiological function of multidrug efflux pumps in Escherichia coli and Salmonella (2009)
  • Author(s): Nishino K., Nikaido E., Yamaguchi A.
  • Journal Title: Biochim. Biophys. Acta 1794 Pages: 834-843
  • Peer Reviewed
• [Journal Article] H-NS modulates multidrug resistance of Salmonella enterica serovar Typhimurium by repressing acrEF multidrug efflux genes. (2009)
  • Author(s): Nishino, K., et al.
  • Journal Title: Antimicrob. Agents Chemother. 53 Pages: 3541-3543
  • Peer Reviewed
• [Journal Article] Role of the AraC/XylS family regulator YdeO in multidrug resistance of Escherichia coli. (2009)
  • Author(s): Nishino, K., et al.
  • Journal Title: J.Antibiot. 62 Pages: 251-257
  • NAID: 10030635180
  • Peer Reviewed
• [Journal Article] Regulation and physiological function of multidrug efflux pumps in Escherichia coli and Salmonella. (2009)
  • Author(s): Nishino, K., et al.
  • Journal Title: Biochim. Biophys. Acta. 1794 Pages: 834-843
  • Peer Reviewed
• [Journal Article] Regulation of the AcrAB multidrug efflux pump in Salmonella enterica serovar Typhimurium. (2009)
  • Author(s): Nikaido, E., et al.
  • Journal Title: Recent Advances in Clinical Pharmacology 30 Pages: 77-84
• [Journal Article] Roles of drug efflux pumps in bacterial multidrug resistance and virulence. (2009)
  • Author(s): Nishino, K.
  • Journal Title: Reviews & Topics on Surface Science and Technology Avant-Gardes 47 Pages: 235-245
  • NAID: 40016813649
• [Journal Article] 新たな創薬ターゲットとしての薬剤排出ポンプ (2009)
  • Author(s): 西野邦彦
  • Journal Title: Jpn. J.Chemother. 57(A) Pages: 81-81
• [Journal Article] 排出ポンプ阻害活性と膜傷害作用の分離評価 (2009)
  • Author(s): 松本佳巳, 他
  • Journal Title: Jpn. J.Chemother. 57(A) Pages: 225-225
• [Journal Article] Regulation of the AcrAB multidrug efflux pump in Salmonella enterica serovar Typhimurium. (2009)
  • Author(s): Nikaido E., et al.
  • Journal Title: Recent Advances in Clinical Pharmacology (in press)
• [Journal Article] Regulation and physiological function of multidrug effluxpumps in Escherichia coli and Salmonella. (2009)
  • Author(s): Nishino, K., et al.
  • Journal Title: BBA-Proteins and Proteomics (in press)
  • Peer Reviewed
• [Journal Article] AcrAB multidrug efflux pump regulation in Salmonella enterica serovar Typhimurium by RamA in response to environmental signals (2008)
  • Author(s): Nikaido E., Yamaguchi A., Nishino K.
  • Journal Title: J. Biol. Chem 283 Pages: 24245-24253
  • Peer Reviewed
• [Journal Article] AcrAB multidrug efflux pump regulation in Salmonella enterica serovar Typhimurium by RamA in response to environmental signals. (2008)
  • Author(s): Nikaido, E., et al.
  • Journal Title: J. Biol. Chem. 283 Pages: 24245-24253
  • Peer Reviewed
• [Journal Article] AcrS/EnvR represses the expression of the acrAB multidrug efflux genes in Escherichia coli. (2008)
  • Author(s): Hirakawa, H., et al.
  • Journal Title: J. Bacteriol. 190 Pages: 6276-6279
  • Peer Reviewed
• [Journal Article] CRP regulator modulates multidrug resistance of Escherichia coli by repressing expression of the mdtEF multidrug efflux genes. (2008)
  • Author(s): Nishino, K., et al.
  • Journal Title: J. Antibiot. 61 Pages: 120-127
  • Peer Reviewed
• [Journal Article] The AraC-family regulator GadX enhances multidrug resistance of Escherichia coli by activating expression of the mdtEF multidrug efflux genes. (2008)
  • Author(s): Nishino, K., et al.
  • Journal Title: J. Infect. Chemother. 14 Pages: 23-29
  • NAID: 10021193134
  • Peer Reviewed
• [Journal Article] Identification of a spermidine excretion protein complex (MdtJI) in Escherichia coli. (2008)
  • Author(s): Higashi, K., et al.
  • Journal Title: J. Bacteriol. 190 Pages: 872-878
  • Peer Reviewed
• [Journal Article] Virulence and drug resistance roles of multidrug efflux pumps in Escherichia coli and Salmonella. (2008)
  • Author(s): Nishino, K., et al.
  • Journal Title: Bioscience and Microflora 27 Pages: 75-85
  • NAID: 10021956022
  • Peer Reviewed
• [Journal Article] Physiological functions of drug efflux systems in Gram-negative bacteria : Their roles in bacterial drug resistance and virulence. (2008)
  • Author(s): Nishino, K., et al.
  • Journal Title: Japanese Journal of Chemotherapy 56 Pages: 443-452
  • NAID: 10022594782
  • Peer Reviewed
• [Journal Article] Role of xenobiotic transporters in bacterial drug resistance and virulence. (2008)
  • Author(s): Nishino, K., et al.
  • Journal Title: IUBMB Life 60 Pages: 569-574
  • Peer Reviewed
• [Journal Article] Roles of drug efflux pumps in bacterial multidrug resistance and virulence-Analysis to identify novel drug targets and counteract multidrug resistance and virulence -. (2008)
  • Author(s): Nishino, K.
  • Journal Title: Japanese Journal of Antibiotics 61 Pages: 105-113
  • NAID: 10021928431
• [Journal Article] Roles of efflux pumps in bacterial virulence and multidrug resistance. (2008)
  • Author(s): Nishino, K.
  • Journal Title: Antibiotics & Chemotherapy 24 Pages: 354-362
• [Journal Article] Resistome analysis of bacterial transportsome. (2008)
  • Author(s): Nishino, K., et al.
  • Journal Title: Journal of the Pharmaceutical Society of Japan 128 (2) Pages: 73-74
  • Peer Reviewed
• [Journal Article] Role of multidrug efflux pumps in protection of cell membrane. (2008)
  • Author(s): Sakata, H., et al.
  • Journal Title: Journal of the Pharmaceutical Society of Japan 127(2) Pages: 123-124
  • Peer Reviewed
• [Journal Article] Electron tomographic reconstruction of clathrin coated endocytic intermediates in nerve terminals that lack dynamin1. (2008)
  • Author(s): Nishino-Hayashi, M., et al.
  • Journal Title: Journal of the Pharmaceutical Society of Japan 127(2) Pages: 143-144
  • Peer Reviewed
• [Journal Article] Roles of multidrug efflux pumps in Escherichia coli and Salmonella. (2008)
  • Author(s): Nishino, K.
  • Journal Title: Journal of Intestinal Microbiology 22 Pages: 63-64
  • NAID: 10024187243
• [Journal Article] Identification of a spermidine excretion protein complex (MdtJI) in Escherichia coli (2008)
  • Author(s): Higashi K, Ishigure H, Demizu R, Uemura. T, Nishno K, Yamaguchi A, Kashiwagi K, Igarashi K
  • Journal Title: J. Bacteriol. 190 Pages: 872-878
  • Peer Reviewed
• [Journal Article] The AraC-family regulator GadX enhances multidrug resistance of Escherichia coli by activating expression of the mdtEF multidrug efflux genes (2008)
  • Author(s): Nishino K, Senda Y, Yamaguchi A
  • Journal Title: J. Infect. Chemother. 14 Pages: 23-29
  • NAID: 10021193134
  • Peer Reviewed
• [Journal Article] Roles of drug efflux pumps in bacterial multidrug resistance and virulence-Analysis to identify novel drug targets and counteract multidrug resistance and virulence- (2008)
  • Author(s): Nishino K
  • Journal Title: Jpn. J. Antibiot (In press)
  • NAID: 10021928431
• [Journal Article] Roles of efflux pumps in bacterial virulence and multidrug resistance (2008)
  • Author(s): Nishino K
  • Journal Title: Antibiotics & Chemotherapy 24 Pages: 354-362
• [Journal Article] CRP regulator modulates multidrug resistance of Escherichia coli by repressing expression of the mdtEF multidrug efflux genes (2008)
  • Author(s): Nishino K, Senda Y, Yamaguchi A
  • Journal Title: J. Antibiot (In press)
  • Peer Reviewed
• [Journal Article] Role of xenobiotic transporters in bacterial drug resistance and virulence (2008)
  • Author(s): Nishino K, Yamaguchi A
  • Journal Title: IUBMB Life (In press)
  • Peer Reviewed
• [Journal Article] Regulation of multidrug efflux systems involved in multidrug and metal resistance of Salmonella enterica (2007)
  • Author(s): Nishino K., Nikaido E., Yamaguchi A.
  • Journal Title: J. Bacteriol 189 Pages: 9066-9075
  • Peer Reviewed
• [Journal Article] Regulation of multidrug efflux systems involved in multidrug and metal resistance of Salmonella enterica (2007)
  • Author(s): Nishino K, Nikaido E, Yamaguchi A
  • Journal Title: J. Bacteriol. 189 Pages: 9066-9075
  • Peer Reviewed
• [Journal Article] Rapid detection of drug efflux from single bacterial cell enclosed in femtoliter chamber array (2007)
  • Author(s): Iino R, Nishino K, Nakata M, Nikaido E, Matsumoto Y, Sakakihara S, Takeuchi S, Yamaguchi A, Noji H
  • Journal Title: Micro Total Analysis Systems 1 Pages: 757-759
  • Peer Reviewed
• [Presentation] 多剤排出ポンプによる細菌機能制御 (2011)
  • Author(s): 西野邦彦
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: 静岡
  • Year and Date: 2011-03-29
• [Presentation] Development of novel therapeutic strategies to tackle multidrug-resistant pathogens (2011)
  • Author(s): Nishino, K., et al.
  • Organizer: The American Association for the Advancement of Science (AAAS) 2011 Annual Meeting
  • Place of Presentation: Washington, DC, USA
  • Year and Date: 2011-02-20
• [Presentation] 排出ポンプ阻害剤Phe-Arg-β-naphthylamide (PAN)の作用メカニズム (2011)
  • Author(s): 松本佳巳, 他
  • Organizer: 第45回緑膿菌感染症研究会
  • Place of Presentation: 仙台
• [Presentation] Analysis of Salmonella AcrAB multidrug efflux pump regulator : RamR (2010)
  • Author(s): 二階堂英司, 他
  • Organizer: 第33回日本分子生物学会・第83回日本生化学会大会 合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] Role of the RNA chaperon Hfq in multidrug resistance of Escherichia coil (2010)
  • Author(s): Nishino, K., et al.
  • Organizer: New Zealand Microbiological Society and New Zealand Society for Biocheistry & Molecular Biology Joint Meeting 2010
  • Place of Presentation: Auckland, New Zealand
  • Year and Date: 2010-12-01
• [Presentation] 細菌多剤耐性化・病原性発現制御機構の解明と新規治療法開発 (2010)
  • Author(s): 西野邦彦
  • Organizer: 大阪大学産業科学研究所第66回学術講演会
  • Place of Presentation: 大阪
  • Year and Date: 2010-11-22
• [Presentation] サルモネラ異物排出トランスポーターAcrAB発現制御因子RamRのX線結晶構造解析 (2010)
  • Author(s): 二階堂英司, 他
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2010-09-21
• [Presentation] 多剤排出ポンプの多彩な機能 (2010)
  • Author(s): 西野邦彦
  • Organizer: 第58回日本化学療法学会総会
  • Place of Presentation: 長崎
  • Year and Date: 2010-06-04
• [Presentation] Virulence and drug resistance roles of multidrug efflux systems in Salmonella enterica (2010)
  • Author(s): Nishino, K.
  • Organizer: ASM American Society for Microbiology 110th General Meeting
  • Place of Presentation: San Diego, USA
  • Year and Date: 2010-05-25
• [Presentation] Electron tomography revealed the endoplasmic reticulum as a source of autophagosomal membranes (2010)
  • Author(s): Nishino-Hayashi, M., et al.
  • Organizer: ASM American Society for Microbiology 110th General Meeting
  • Place of Presentation: San Diego, USA
  • Year and Date: 2010-05-24
• [Presentation] 大腸菌マクロライド排出蛋白質の膜局在. (2010)
  • Author(s): 平田隆弘, 他
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-30
• [Presentation] 大腸菌薬剤排出ポンプ制御におけるNlpEの役割. (2010)
  • Author(s): 山崎聖司, 他
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-28
• [Presentation] サルモネラ異物排出トランスポーターAcrABの発現制御ネットワーク解析. (2010)
  • Author(s): 二階堂英司, 他
  • Organizer: 第83回細菌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2010-03-27
• [Presentation] Regulation of multidrug efflux pumps in Escherichia coli. (2010)
  • Author(s): Yamasaki, S., et al.
  • Organizer: The 10th Japan-Korea International Symposium on Microbiology
  • Place of Presentation: Yokohama
  • Year and Date: 2010-03-26
• [Presentation] Physiological functions of multidrug efflux pumps in Salmonella. (2010)
  • Author(s): Nishino, K.
  • Organizer: The 10th Japan-Korea International Symposium on Microbiology
  • Place of Presentation: Yokohama
  • Year and Date: 2010-03-26
• [Presentation] Regulatory network and physiological function of bacterial multidrug transporters (2010)
  • Author(s): Yamasaki, S., et al.
  • Organizer: 物質・デバイス領域共同研究拠点発足記念シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2010-03-24
• [Presentation] Electron tomography revealed a subdomain of the endoplasmic reticulum as a cradle for autophagosome formation. (2010)
  • Author(s): Hayashi-Nishino, M., et al.
  • Organizer: 物質・デバイス領域共同研究拠点発足記念シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2010-03-24
• [Presentation] Green Microbiology : Eggects of N1pE overproduction on the induction of xenobiotic transporters involved in multidrug resistance in Escherichia coli. (2010)
  • Author(s): Yamasaki, S., et al.
  • Organizer: The 13th SANKEN International Symposium
  • Place of Presentation: Osaka
  • Year and Date: 2010-01-18
• [Presentation] Green Microbiology : Development of novel therapeutic strategies to tackle multidrug-resistant pathogens. (2010)
  • Author(s): Nishino, K.
  • Organizer: The 13th SANKEN International Symposium
  • Place of Presentation: Osaka
  • Year and Date: 2010-01-18
• [Presentation] 排出ポンプ阻害剤Phe-Arg-β-naphthylamide (PAN)の外膜障害作用 (2010)
  • Author(s): 松本佳巳, 他
  • Organizer: 第39回薬剤耐性菌研究会
  • Place of Presentation: 群馬
• [Presentation] 大腸菌を用いた緑膿菌のRND型排出ポンプMexABおよびMexXYの活性測定. (2010)
  • Author(s): 松本佳巳, 他
  • Organizer: 第44回緑膿菌感染症研究会
  • Place of Presentation: 東京
• [Presentation] 病原菌に潜む薬剤耐性・病原性制御ネットワークと新規治療法開発. (2009)
  • Author(s): 西野邦彦.
  • Organizer: 大阪大学産業科学研究所創立70周年記念講演会・第65回学術講演会
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-03
• [Presentation] Electron tomography revealed a subdomain of the endoplasmic reticulum as a eradle for autophagosome formation. (2009)
  • Author(s): Hayashi-Nishino, M., et al.
  • Organizer: 第31回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-01
• [Presentation] Virulence and drug resistance roles of bacterial multidrug efflux systems. (2009)
  • Author(s): Nishino, K.
  • Organizer: 第31回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2009-11-30
• [Presentation] Regulation mechanism of Salmonella AcrAB multidrug efflux pump in response to extracellular signals. (2009)
  • Author(s): Nikaido, E., et al.
  • Organizer: 第31回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2009-11-30
• [Presentation] The localization study of a bacterial efflux transporter. (2009)
  • Author(s): Hirata, T., et al.
  • Organizer: 第31回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2009-11-30
• [Presentation] サルモネラ多剤排出トランスポーターの生理機能解明. (2009)
  • Author(s): 西野邦彦, 他
  • Organizer: 第2回日本化学療法学会西日本支部長賞受賞講演・第57回日本化学療法学会西日本支部総会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-11-28
• [Presentation] Fluorescein di-ss-D-galactopyranoside (FDG)を用いた排出ポンプ阻害剤の評価. (2009)
  • Author(s): 松本佳巳, 他
  • Organizer: 第38回薬剤耐性菌研究会
  • Place of Presentation: 群馬
  • Year and Date: 2009-11-20
• [Presentation] 外部シグナルによるRamAを介したサルモネラ遺伝子発現制御機構の解析. (2009)
  • Author(s): 二階堂英司, 他
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-24
• [Presentation] 異物排出トランスポーターによる宿主免疫回避機構の解明. (2009)
  • Author(s): 上田珠美, 他
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-22
• [Presentation] Effects of lipoprotein overproduction on the induction of xenobiotic transporters involved in multidrug resistance in Salmonella enterica. (2009)
  • Author(s): Shirosaka, I., et al.
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-22
• [Presentation] Roles of xenobiotic transporters in bacterial drug resistance and virulence. (2009)
  • Author(s): Nishino, K., A.Yamaguchi.
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-22
• [Presentation] 大腸菌RND型薬剤排出ポンプの進化. (2009)
  • Author(s): 田中真奈美, 他
  • Organizer: 第11回日本進化学会大会
  • Place of Presentation: 札幌
  • Year and Date: 2009-09-03
• [Presentation] Function and regulation of multidrug efflux pumps in E. coli and Salmonella. (2009)
  • Author(s): Nishino, K.
  • Organizer: School of Biological Sciences Seminar at the University of Hong Kong
  • Place of Presentation: 香港
  • Year and Date: 2009-08-28
• [Presentation] 排出ポンプ阻害活性と膜傷害作用の分離評価. (2009)
  • Author(s): 松本佳巳, 他
  • Organizer: 第57回日本化学療法学会総会
  • Place of Presentation: 東京
  • Year and Date: 2009-06-05
• [Presentation] 新たな創薬ターゲットとしての薬剤排出ポンプ. (2009)
  • Author(s): 西野邦彦
  • Organizer: 第57回日本化学療法学会総会
  • Place of Presentation: 東京
  • Year and Date: 2009-06-04
• [Presentation] Unexpected role of multidrug efflux pumps in Salmonella virulence. (2009)
  • Author(s): Nishino, K
  • Organizer: 3rd Symposium on Antimicrobial Resistance in Animals and the Environment
  • Place of Presentation: Tours, France
  • Year and Date: 2009-06-02
• [Presentation] Microarray analysis of Salmonella enterica during exposure to environmental signals reveals new targets of the RamA regulatory protein. (2009)
  • Author(s): Nikaido, E., et al.
  • Organizer: American Society for Microbiology 109th General Meeting
  • Place of Presentation: Philadelphia, U.S.A.
  • Year and Date: 2009-05-19
• [Presentation] Physiological functions of multi-drug efflux systems in S. enterica. (2009)
  • Author(s): Nishino, K.
  • Organizer: The Gordon Research Conference on Multi-Drug Efflux Systems
  • Place of Presentation: Texas, USA
  • Year and Date: 2009-03-23
• [Presentation] 宿主免疫回避におけるサルモネラ薬剤排出ポンプの生理的役割 (2009)
  • Author(s): 西野邦彦, 他
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-12
• [Presentation] サルモネラ異物排出トランスポーターAcrAB発現制御機構の解析 (2009)
  • Author(s): 二階堂英司, 他
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-12
• [Presentation] インドールによるサルモネラ異物排出トランスポーターAcrAB発現制御機構の解析 (2009)
  • Author(s): 城阪郁江, 他
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-12
• [Presentation] 細胞侵入と細胞内増殖におけるサルモネラ異物排出トランスポーターの役割 (2009)
  • Author(s): 坂田博樹, 他
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-12
• [Presentation] AcrAB-TolC exported energy-dependently permeated Fluorescein-di-β-D-galactopyranoside. (2009)
  • Author(s): 松本佳巳, 他
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-12
• [Presentation] AcrAB multidrug efflux pump regulation in Salmonella enterica serovar Typhimurium in response to indole. (2009)
  • Author(s): Sirosaka, I., et al.
  • Organizer: The 12^<th> Sanken International Symposium
  • Place of Presentation: Osaka
  • Year and Date: 2009-01-22
• [Presentation] Physiological functions of multi-drug efflux systems in S. enterica (2009)
  • Author(s): Nishino, K.
  • Organizer: The Gordon Research Conference on Multi-Drug Efflux Systems
  • Place of Presentation: Texas, USA
• [Presentation] サルモネラ異物排出トランスポーターAcrAB誘導に関与するRamAおよびRamRの解析. (2009)
  • Author(s): 城阪郁江, 他
  • Organizer: 第9回日本蛋白質科学会年会
  • Place of Presentation: 熊本
• [Presentation] Roles of xenobiotic transporters in bacterial drug resistance and virulence. (2009)
  • Author(s): Nishino, K., A.Yamaguchi.
  • Organizer: The Awaji International Forum on Infection and Immunity
  • Place of Presentation: Awaji Island
• [Presentation] Membrane-damaging activity of Phe-Arg-ss-Naphthylamide in Escherichia coli. (2009)
  • Author(s): Matsumoto, Y., et al.
  • Organizer: American Society for Microbiology 49th ICAAC Meeting
  • Place of Presentation: San Francisco, CA, U.S.A.
• [Presentation] 外部シグナルによるサルモネラ異物排出トランスポーターAcrAB発現制御メカニズム解析. (2009)
  • Author(s): 二階堂英司, 他
  • Organizer: 第3回細菌学・若手コロッセウム
  • Place of Presentation: 宮崎
• [Presentation] Roles of drug efflux pumps in bacterial multidrug resistance and virulence. (2009)
  • Author(s): Nishino, K.
  • Organizer: 3^<rd> Annual Japanese-French Frontiers of Science Symposium
  • Place of Presentation: Kanagawa, Japan
• [Presentation] 緑膿菌におけるmexB, mexYおよびoprDの発現量と薬剤感受性. (2009)
  • Author(s): 松本佳巳, 他
  • Organizer: 第43会緑膿菌感染症研究会
  • Place of Presentation: 京都
• [Presentation] Regulatory network of multidrug transporters reveals their physiological role in Salmonella virulence. (2008)
  • Author(s): Nishino, K., et al.
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] 抗菌ペプチド耐性における多剤排出ポンプの役割 (2008)
  • Author(s): 中濃知志, 他
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] RamAによるサルモネラ異物排出トランスポーターAcrAB発現制御機構の解析 (2008)
  • Author(s): 二階堂英司, 他
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] 多剤耐性緑膿菌(MDRP)におけるmexB, mexYおよびoprDの発現量 (2008)
  • Author(s): 松本佳巳, 他
  • Organizer: 第56回日本化学療法学会西日本支部総会
  • Place of Presentation: 広島
  • Year and Date: 2008-12-07
• [Presentation] サルモネラ薬剤感受性に対する多剤排出ポンプインヒビターPhe-Argβ-naphthylamide (PAN)の影響 (2008)
  • Author(s): 小川美保, 他
  • Organizer: 第56回日本化学療法学会西日本支部総会
  • Place of Presentation: 広島
  • Year and Date: 2008-12-07
• [Presentation] 抗菌ペプチド耐性における多剤排出ポンプの役割 (2008)
  • Author(s): 中濃知志, 他
  • Organizer: 第61回日本細菌学会関西支部総会
  • Place of Presentation: 京都
  • Year and Date: 2008-11-08
• [Presentation] サルモネラの薬剤感受性における多剤排出ポンプの関与 (2008)
  • Author(s): 小川美保, 他
  • Organizer: 第37回薬剤耐性菌研究会
  • Place of Presentation: 群馬
  • Year and Date: 2008-09-12
• [Presentation] 能動的に取り込まれたFluoresein-β-D-galactopyranoside(FDG)を排出する大腸菌のRND排出ポンプ (2008)
  • Author(s): 葉山浩平, 他
  • Organizer: 第37回薬剤耐性菌研究会
  • Place of Presentation: 群馬
  • Year and Date: 2008-09-12
• [Presentation] AcrAB multidrug efflux pump regulation in Salmonella enterica serovar Typhimurium by RamA in response to environmental signals. (2008)
  • Author(s): Nishino, K., et al.
  • Organizer: The Awaji International Forum on Infection and Immunity
  • Place of Presentation: Awaji Island
  • Year and Date: 2008-09-10
• [Presentation] 細菌トランスポートソームのレジストーム解析 (2008)
  • Author(s): 西野邦彦, 他
  • Organizer: 第30回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 札幌
  • Year and Date: 2008-08-08
• [Presentation] 膜傷害保護における異物排出トランスポーターの役割 (2008)
  • Author(s): 坂田博樹, 他
  • Organizer: 第30回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 札幌
  • Year and Date: 2008-08-07
• [Presentation] 電子線トモグラフィーを用いたダイナミン1KOシナプスにおけるエンドサイトーシス中間体の解析 (2008)
  • Author(s): 西野-林美都子, 他
  • Organizer: 第30回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 札幌
  • Year and Date: 2008-08-07
• [Presentation] サルモネラ異物排出トランスポーターAcrABの発現制御と環境応答におけるRamAの役割 (2008)
  • Author(s): 二階堂英司, 他
  • Organizer: 第2回細菌学・若手コロッセウム
  • Place of Presentation: 湘南
  • Year and Date: 2008-08-03
• [Presentation] Regulatory network of multidrug efflux pumps reveals their physiological role in Salmonella virulence. (2008)
  • Author(s): Nishino, K., et al.
  • Organizer: The Gordon Research Conference
  • Place of Presentation: South Hadley, MA, U.S.A.
  • Year and Date: 2008-07-09
• [Presentation] Roles of multidrug efflux pumps in Escherichia coli and Salmonella. (2008)
  • Author(s): 西野邦彦
  • Organizer: The 12^<th> Annual Meeting of Intestinal Microbiology(受賞講演)
  • Place of Presentation: 東京
  • Year and Date: 2008-06-12
• [Presentation] フェムトリッター(fL)チャンバーを用いた薬剤排出活性測定法を用いたインヒビターの評価 (2008)
  • Author(s): 松本佳巳, 他
  • Organizer: 第56回日本化学療法学会総会
  • Place of Presentation: 岡山
  • Year and Date: 2008-06-06
• [Presentation] Regulatory network of multidrug transporters reveals their physiological role in Salmonella virulence. (2008)
  • Author(s): Nishino, K., et al.
  • Organizer: American Society for Microbiology 108th General Meeting
  • Place of Presentation: Boston, USA
  • Year and Date: 2008-06-03
• [Presentation] Regulation of the AcrAB multidrug efflux pump in Salmonella enterica by RamA in response to environmental signals. (2008)
  • Author(s): Nikaido, E., et al.
  • Organizer: American Society for Microbiology 108th General Meeting
  • Place of Presentation: Boston, USA
  • Year and Date: 2008-06-02
• [Presentation] 排出機構-多剤耐性化と病原因子のキー・プレイヤー- (2008)
  • Author(s): 西野邦彦
  • Organizer: 第82回日本感染症学会総会
  • Place of Presentation: 島根
  • Year and Date: 2008-04-17
• [Presentation] 金属耐性におけるサルモネラ菌多剤排出トランスポーターの役割 (2008)
  • Author(s): 西野邦彦、二階堂 英司、山口 明人
  • Organizer: 日本薬学会 第128年会
  • Place of Presentation: 横浜
  • Year and Date: 2008-03-28
• [Presentation] サルモネラ異物排出トランスポーターAcrAB制御因子の同定 (2008)
  • Author(s): 二階堂英司、西野邦彦、山口明人
  • Organizer: 日本薬学会 第128年会
  • Place of Presentation: 横浜
  • Year and Date: 2008-03-28
• [Presentation] サルモネラ異物排出トランスポーターEmrABの機能解析 (2008)
  • Author(s): 坂田博樹、西野邦彦、山口明人
  • Organizer: 日本薬学会 第128年会
  • Place of Presentation: 横浜
  • Year and Date: 2008-03-28
• [Presentation] 大腸菌薬剤排出タンパク質の細胞内局在 (2008)
  • Author(s): 平田隆弘、北村昭夫、西野邦彦、山口明人
  • Organizer: 日本薬学会 第128年会
  • Place of Presentation: 横浜
  • Year and Date: 2008-03-28
• [Presentation] サルモネラ異物排出トランスポーターAcrAB生理的役割と発現誘導機構の解析 (2008)
  • Author(s): 二階堂英司、西野邦彦、山口明人
  • Organizer: 第81回日本細菌学会総会
  • Place of Presentation: 京都
  • Year and Date: 2008-03-26
• [Presentation] サルモネラ異物排出トランスポーターEmrABの機能解析 (2008)
  • Author(s): 坂田博樹、西野邦彦、山口明人
  • Organizer: 第81回日本細菌学会総会
  • Place of Presentation: 京都
  • Year and Date: 2008-03-26
• [Presentation] 異物排出蛋白質発現抑制因子AcrSの機能解明とAcrAB,AcrEFの発現制御 (2008)
  • Author(s): 平川秀忠、西野邦彦、平田隆弘、山口明人
  • Organizer: 第81回日本細菌学会総会
  • Place of Presentation: 京都
  • Year and Date: 2008-03-24
• [Presentation] サルモネラ菌薬剤排出ポンプの生理機能-多剤耐性および病原性発現における役割について- (2008)
  • Author(s): 西野邦彦
  • Organizer: 第81回日本細菌学会総会
  • Place of Presentation: 京都
  • Year and Date: 2008-03-24
• [Presentation] Roles of multidrug efflux pumps in antimicrobial peptide resistance of Salmonella enterica (2008)
  • Author(s): Nakano, T., K. Nishino and A. Yamaguchi
  • Organizer: Florence Conference on Phenotype MicroArray Analysis of Microorganisms
  • Place of Presentation: Florence, Italy
  • Year and Date: 2008-03-19
• [Presentation] Regulatory network of AcrAB multidrug efflux pump Salmonl1a and its role in response to metabolites (2008)
  • Author(s): Nikaido E., K. Nishino and A.Yamaguchi
  • Organizer: Florence Conference on Phenotype MicroArray Analysis of Microoreanisms
  • Place of Presentation: Florence, Italy
  • Year and Date: 2008-03-19
• [Presentation] Physiological functions of bacterial multidrug efflux pumps (2008)
  • Author(s): Kunihiko Nishino
  • Organizer: The 3rd CNU-SANKEN Joint Symposium on Advanced Materials Science
  • Place of Presentation: Chungnam National University, Korea
  • Year and Date: 2008-02-27
• [Presentation] Regulation mechanism of the AcrAB multidrug efflux pump in Salmonella enterica in response to bacterial metabolites and bile (2008)
  • Author(s): Nikaido, E., K. Nishino, and A. Yamaguchi
  • Organizer: 11th Sanken, 6th Nanotechnology Center and 1st MSTEC International Symposium
  • Place of Presentation: Awaji Island
  • Year and Date: 2008-02-04
• [Presentation] 膜傷害保護における異物排出トランスポーターの役割 (2008)
  • Author(s): 坂田博樹, 他
  • Organizer: BMB2008
  • Place of Presentation: 神戸([ポスター発表・口頭発表採択])
• [Presentation] Phenotypic analysis of multidrug efflux pumps-not just for multidrug resistance (2008)
  • Author(s): Kunihiko Nishino
  • Organizer: Florence Conference on Phenotype MicroArray Analysis of Microorganisms
  • Place of Presentation: Florence, Italy
• [Presentation] サルモネラ菌異物排出トランスポーターAcrAB発現誘導機構の解析 (2007)
  • Author(s): 二階堂英司、西野邦彦、山口明人
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14
• [Presentation] サルモネラ菌異物排出トランスポーターEmrABの機能解析 (2007)
  • Author(s): 坂田博樹、西野邦彦、山口明人
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14
• [Presentation] 大腸菌バイオフィルム形成機構に関与する新規遺伝子産物に関する研究 (2007)
  • Author(s): 王麗媛、平田隆弘、平川秀忠、西野邦彦、山口明人
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14
• [Presentation] The regulatory mechanism of the AcrAB and AcrEF multidrug exporter system by AcrS, in Escherichia coli (2007)
  • Author(s): 平川秀忠、Ulrike Theisen, 小林あすか、平田隆弘、西野邦彦、山口明人
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14
• [Presentation] 細菌薬剤耐性化と病原性発現における薬剤排出トランスポーターの役割 (2007)
  • Author(s): 西野邦彦
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
• [Presentation] 大腸菌における新規ポリアミン排出蛋白質の同定 (2007)
  • Author(s): 東恭平、石槫博之、出水梨沙、植村武史、戸井田敏彦、柏木敬子、西野邦彦、山口明人、五十嵐一衛
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] 細菌代謝物によるサルモネラ菌異物排出トランスポーターAcrAB-To1C シスラム発現制御機構の解析 (2007)
  • Author(s): 二階堂英司、西野邦彦、山口明人
  • Organizer: 第29回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 仙台
  • Year and Date: 2007-11-26
• [Presentation] 細菌多剤耐性化と病原性発現における薬剤排出ポンプの役割に関する研究-多剤耐性化と病原性を軽減させる新薬ターゲットの解析- (2007)
  • Author(s): 西野邦彦
  • Organizer: 日本抗生物質学術協議会奨励賞受賞講演
  • Place of Presentation: 東京
  • Year and Date: 2007-11-08
• [Presentation] Single cell measurement of efflux activity of multidrug transporters enclosed in femtoliter chamber array (2007)
  • Author(s): 仲田昌義、飯野亮太、西野邦彦、二階堂英司、松本佳巳、榊原昇一、竹内昌治、山口明人、野地博行
  • Organizer: 第45回生物物理学会年会
  • Place of Presentation: 横浜
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