Development of new-generation gene vectors by a control of intracellular trafficking and dispersion based in a real-time imaging of DNA
Project/Area Number |
19680025
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Research Category |
Grant-in-Aid for Young Scientists (A)
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Allocation Type | Single-year Grants |
Research Field |
Biomedical engineering/Biological material science
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Research Institution | Hokkaido University |
Principal Investigator |
AKITA Hidetaka Hokkaido University, 大学院・薬学研究院, 助教 (80344472)
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Project Period (FY) |
2007 – 2009
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Project Status |
Completed (Fiscal Year 2009)
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Budget Amount *help |
¥25,610,000 (Direct Cost: ¥19,700,000、Indirect Cost: ¥5,910,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2008: ¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000)
Fiscal Year 2007: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
|
Keywords | リアルタイムイメージング / 細胞内動態制御 / 遺伝子デリバリー |
Research Abstract |
In this project, intracellular trafficking of nano-carrier was controlled from the point of view of intracellular dynamics (i.e. vesicular transport and/or microtubule-dependent transport), dispersity and homogeneity to improve the transfection activity. These technologies will be valuable as used in DNA vaccine and liver-targeting gene carriers. Furthermore, novel peptide was discovered, which confer the activity to pass through the cell monolayer via transcytosis pathway. This device will be useful to overcome the vesicular barriers which limits the delivery to tissue parenchyma.
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Report
(4 results)
Research Products
(36 results)
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[Journal Article] Nanoparticles for ex vivo siRNA delivery to dendritic cells for cancer vaccines : Programmed endosomal escape and dissociation2010
Author(s)
Akita H, Kogure K, Moriguchi R, Nakamura Y, Higashi T, Nakamura T, Serada S, Fujimoto M, Naka T, Futaki S, Harashima H.
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Journal Title
J Control Release. (in press)
Related Report
Peer Reviewed
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[Journal Article] Multi-layered nano particles for penetrating the endosome and nuclear membrane via a step-wise membrane fusion process.2009
Author(s)
H.Akita, A.Kudo, A.Minoura, M.Yamaguchi, I.A Khalil, R.Moriguchi, T.Masuda, R.Danev, K.Nagayama, K.Kogure, H.Harashima
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Journal Title
Biomaterials 30
Pages: 2940-2949
Related Report
Peer Reviewed
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[Journal Article] Envelope-type lipid nanoparticles incorporating a short PEG-lipid conjugate for improved control of intracellular trafficking and transgene transcription2009
Author(s)
Masuda, T., Akita, H., Niikura, K., Nishio, T., Ukawa, M., Enoto, K., Danev, R., Nagayama, K., Ijiro, K., Harashima, H
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Journal Title
Biomaterials 30
Pages: 4806-4814
Related Report
Peer Reviewed
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[Journal Article] Nanoparticles for ex vivo siRNA delivery to dendritic cells for cancer vaccines
Author(s)
H.Akita, K.Kogure, R.Moriguchi, Y.Nakamura, T.Higashi, T.Nakamura, S.Serada, M.Fujimoto, T.Naka, S.Futaki, H.Harashima
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Journal Title
Programmed endosomal escape and dissociation (in press)
Related Report
Peer Reviewed
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