Study on bio-polyester synthesis from recycled alkenoic acids

• Project/Area Number: 19681008
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Environmental technology/Environmental materials
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: TSUGE Takeharu Tokyo Institute of Technology, 大学院・総合理工学研究科, 准教授 (70332260)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2007: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
• Keywords: ポリヒドロキシアルカン酸 / 酵素合成 / 連鎖移動 / ケミカルリサイクル / 不飽和脂肪酸 / 熱分解

Research Abstract

The aim of this study is to develop the chemical recycling system of polyhydroxyalkanoate (PHA) by integrating pyrolysis and enzymatic or microbial synthesis of PHA. The major product obtained from pyrolysis of PHA, alkenoic acids, is the key material of this recycling system. We demonstrated that alkenoic acids are able to be re-synthesized to original PHA.

Research Products

• [Journal Article] In vitro synthesis of polyhydroxyalkanoate catalyzed by class II and III PHA synthases: a useful technique for surface coatings of a hydrophobic support with PHA (2010)
  • Author(s): Sato, S., Minato, M., Kikkawa, Y., Abe, H., Tsuge, T.
  • Journal Title: J. Chem. Technol. Biotechnol. 85 Pages: 779-782
  • Peer Reviewed
• [Journal Article] Polyhydroxyalkanoate film formation and synthase activity during in vitro and in situ polymerization on hydrophobic surfaces (2008)
  • Author(s): Sato S, Ono Y, Mochiyama Y, Sivaniah E, Kikkawa Y, Sudesh K, Hiraishi T, Doi Y, Abe H, Tsuge T
  • Journal Title: Biomacromolecules 9 Pages: 2811-2818
  • Peer Reviewed
• [Journal Article] Chain transfer reaction catalyzed by various polyhydroxyalkanoate synthases with poly(ethylene glycol) as an exogenous chain transfer agent Appl.
  • Author(s): Tomizawa, S., Saito, Y., Hyakutake, M., Nakamura, Y., Abe, H., Tsuge, T
  • Journal Title: Microbiol. Biotechenol. (in press)
  • Peer Reviewed
• [Journal Article] In vitro synthesis of polyhydroxyalkanoate catalyzed by class II and III PHA synthases : a useful technique for surface coatings of a hydrophobic support with PHA
  • Author(s): Sato, S., Minato, M., Kikkawa, Y., Abe, H., Tsuge, T.
  • Journal Title: J.Chem.Technol.Biotechnol. (印刷中)
  • Peer Reviewed
• [Journal Article] Chain transfer reaction catalyzed by various polyhydroxyalkanoate synthases with poly(ethylene glycol)as an exogenous chain transfer agent
  • Author(s): Tomizawa, S., Saito, Y., Hyakutake, M., Nakamura, Y., Abe, H., Tsuge, T.
  • Journal Title: Appl.Microbiol.Biotechenol. (印刷中)
  • Peer Reviewed
• [Presentation] 組換え大腸菌によるアルケン酸からの中鎖ポリヒドロキシアルカン酸生合成 (2010)
  • Author(s): 佐藤俊、石井直樹、阿部英喜、柘植丈治
  • Organizer: 高分子学会、
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2010-05-28
• [Presentation] QCMを用いたPHAナノ薄膜形成過程の解析 (2009)
  • Author(s): 持山由規子、佐藤俊、阿部英喜、柘植丈治
  • Organizer: 日本生物工学会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-09-23
• [Presentation] 熱分解クロトン酸からのポリヒドロキシブタン酸酵素再合成の検討 (2009)
  • Author(s): 吉岡真佐子、佐藤俊、阿部英喜、柘植丈治
  • Organizer: 高分子討論会
  • Place of Presentation: 熊本大学
  • Year and Date: 2009-09-17
• [Presentation] 熱分解クロトン酸からのポリヒドロキシブタン酸酵索再合成の検討 (2009)
  • Author(s): 吉岡真佐子、佐藤俊、阿部英喜、柘植丈治
  • Organizer: 高分子討論会
  • Place of Presentation: 熊本大学
  • Year and Date: 2009-09-17
• [Presentation] 連鎖移動反応を利用した大腸菌産生ポリエステルのBio-PEGylation (2009)
  • Author(s): 富澤哲、阿部英喜、柘植丈治
  • Organizer: 高分子討論会
  • Place of Presentation: 熊本大学
  • Year and Date: 2009-09-16
• [Presentation] 連鎖移動反応を利用した大腸菌産生ポリエステルの Bio-PEGylation (2009)
  • Author(s): 富澤哲、阿部英喜、柘植丈治
  • Organizer: 高分子討論会
  • Place of Presentation: 熊本大学
  • Year and Date: 2009-09-16
• [Presentation] J・アグス:連鎖移動反応を誘発させた大腸菌が合成するポリエステルの分子量 (2008)
  • Author(s): 柘植丈治、富澤哲
  • Organizer: 高分子学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2008-05-29
• [Presentation] ポリエステル重合酵素による疎水表面での重合反応解析 (2008)
  • Author(s): 佐藤俊、持山由規子、吉川佳広、阿部英喜、柘植丈治
  • Organizer: 高分子学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2008-05-29
• [Presentation] 連鎖移動反応を誘発させた大腸菌が合成するポリエステルの分子量 (2008)
  • Author(s): 柘植丈治、富澤哲、J. アグス
  • Organizer: 高分子学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2008-05-29
• [Presentation] QCMを用いたポリヒドロキシアルカン酸in vitro重合反応の解析 (2008)
  • Author(s): 持山由規子、佐藤俊、阿部英喜、柘植丈治
  • Organizer: 高分子学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2008-05-28
• [Presentation] R-ヒドラターゼを利用したポリヒドロキシアルカン酸のin vitro重合とAFM観察 (2007)
  • Author(s): 佐藤俊、小野勇将、吉川佳広、阿部英喜、柘植丈治
  • Organizer: 高分子討論会
  • Place of Presentation: 名古屋工業大学
  • Year and Date: 2007-09-20
• [Presentation] 微生物合成ポリエステルの分子量特性 (2007)
  • Author(s): 柘植丈治、富澤哲、J・アグス、P・カハル、阿部英喜、田口精一
  • Organizer: 高分子討論会
  • Place of Presentation: 名古屋工業大学
  • Year and Date: 2007-09-20
• [Remarks]
  • URL: http://www.iem.titech.ac.jp/tsuge/