A Device for quantitative, single cell analysis with dynamic microarray

• Project/Area Number: 19681015
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Microdevices/Nanodevices
• Research Institution: The University of Tokyo
• Principal Investigator: TAKEUCHI Shoji The University of Tokyo, 生産技術研究所, 准教授 (90343110)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥26,260,000 (Direct Cost: ¥20,200,000、Indirect Cost: ¥6,060,000); Fiscal Year 2008: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000); Fiscal Year 2007: ¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
• Keywords: マイクロ化学システム / マイクロカプセル / 細胞アレイ / MEMS / Lab on a Chip / micro TAS / Dynamic amicroarray / マイクロ流体デバイス / 細胞のカプセル化 / Cell encapsulation / 細胞ビーズ / ダイナミックマイクロアレイ / microTAS / 網羅的解析 / 3D AFFD / 3次元培養

Research Abstract

本研究の目的は、我々がこれまでに発案したダイナミックマイクロアレイを利用して、細胞の網羅的な解析が可能なマイクロ流体システムを実現することである。
一万個レベルの細胞のアレイ化を実現するために、まずは、細胞サイズのビーズのアレイ化を検討した。さらに取り出しに細胞へのダメージを軽減させるためのデザインを検討した。これまでには、レーザの照射によりバブルを発生させていたが、熱に拠るダメージが危惧されてきた。そのため、バブル発生源をサンプルから遠ざける機構を検討した。
また、ここでは、我々が考案したプロセスを用いて、アルギン酸カルシウムゲルによるマイクロビーズを作成し、サイズ調整、細胞の分散化などの条件を導き出し、効率的なカプセル化を行なった。これによって、細胞を均一直径のゲルビーズで囲むことよって、細胞自身の直径のばらつきを軽減でき、効率的にアレイ化することに成功した。

Research Products

• [Journal Article] Three-Dimensional Axisymmetric Flow-Focusing Device using Stereolithography (2009)
  • Author(s): Y. Morimoto, W. H. Tan, and S. Takeuchi
  • Journal Title: Biomedical Microdevices vol. 11, no. 2 Pages: 369-377
• [Journal Article] マイクロ流体デバイスを用いたナノバイオ研究 (2009)
  • Author(s): 竹内昌治
  • Journal Title: 電気学会誌C部門誌 vol.129 Pages: 208-212
  • NAID: 10023999537
• [Journal Article] Microfabricated Mobile Microplates for Handling Single Adherent Cells (2008)
  • Author(s): H. Once, and S. Takeuchi
  • Journal Title: Journal of Micromechanics and Microengineering vol. 18 Pages: 95003-95003
• [Journal Article] MEMS technology for nano-bio research (2008)
  • Author(s): D. Collard, S. Takeuchi H. Fujita
  • Journal Title: Drug Discovery Today vol. 13, no. 21-22 Pages: 989-996
• [Journal Article] Dynamic microarray system with gentle retrieval mechanism for cell encapsulating hydrogel beads (2008)
  • Author(s): W-H. Tan and Shoji Takeuchi
  • Journal Title: Lab on a Chip vol. 7 Pages: 259-266
• [Journal Article] Microfabricated Mobile Microplates for Handling Single Adherent Cells (2008)
  • Author(s): H. Onoe, and S. Takeuchi
  • Journal Title: Journal of Micromechanics and Microengineering 18 Pages: 95003-95003
  • Peer Reviewed
• [Journal Article] MEMS technology for nano-bio research (2008)
  • Author(s): D. Collard, S. Takeuchi, H. Fujita
  • Journal Title: Drug Discovery Today 13 Pages: 989-996
  • Peer Reviewed
• [Journal Article] Dynamic microarray system with gentle retrieval mechanism for cell encapsulating hydrogel beads (2008)
  • Author(s): W-H. Tan and Shoji Takeuchi
  • Journal Title: Lab on a Chip 7 Pages: 259-266
  • Peer Reviewed
• [Journal Article] Dynamic microarray system with gentle retrieval mechanism for cell encapsulating hydrogel beads (2008)
  • Author(s): W-H. Tan and Shoji Takeuchi
  • Journal Title: Lab on a Chip vol.7 Pages: 259-266
  • Peer Reviewed
• [Journal Article] Monodisperse Alginate Hydrogel Microbeads for Cell Encapsulation (2007)
  • Author(s): W-H. Tan and Shoji Takeuchi
  • Journal Title: Advanced Materials vol.19 Pages: 2696-2701
  • Peer Reviewed
• [Journal Article] Monodisperse semi-permeable microcapsules for continuous observation of cells
  • Author(s): Y. Morimoto, W. H. Tan, and S. Takeuchi
  • Journal Title: Lab on a Chip, online
• [Presentation] A Dynamic Microarray with Pneumatic Valves for Selective Trapping and Releasing of Microbeads (2009)
  • Author(s): K. Iwai and S. Takeuchi
  • Organizer: MEMS2009
  • Place of Presentation: Italy, Sorrento
  • Year and Date: 2009-01-26
• [Presentation] Size-controlled islet-cell spheroids for geometric analysis of insulin secretion (2009)
  • Author(s): Y. Tsuda, M. Negishi-Kato and S. Takeuchi
  • Organizer: MEMS2009
  • Place of Presentation: Italy, Sorrento
  • Year and Date: 2009-01-26
• [Presentation] Mobile micro-tissues in monodisperse 3D cell culture beads (2008)
  • Author(s): Y. Tsuda, Y. Morimoto and S. Takeuchi
  • Organizer: IUMRS-ICA 2008
• [Presentation] Size-controlled islet-cell spheroids for geometric analysis of insulin secretion (2008)
  • Author(s): Y. Tsuda, M. Negishi-Kato and S. Takeuchi
  • Organizer: MEMS2009
• [Presentation] A Dynamic Microarray with Pneumatic Valves for Selective Trapping and Releasing of Microbeads (2008)
  • Author(s): K. Iwai and S. Takeuchi
  • Organizer: MEMS2009
• [Presentation] Arraying Single Adherent Cells by Microplate Self-Assembly (2008)
  • Author(s): Hirotaka Ishihara, Kuribayashi Kaori and Shoji Takeuchi
  • Organizer: MEMS2009
• [Presentation] 3D cell culture using monodisperse peptide hydrogel beads (2008)
  • Author(s): Y. Tsuda, Y. Morimoto and S. Takeuchi
  • Organizer: microTAS2008
• [Presentation] Formation of Monodisperse Microsized-Emulsions using an Axisymmetric Flow-Focuisng Device Fabricated by Photolithography and Stereolithography (2008)
  • Author(s): Y. Morimoto, K. Kaori and S. Takeuchi
  • Organizer: microTAS2008
• [Presentation] Dynamic Microfluidic Devices with Nanochannels for the Array of 1Micron-Sized Objects (2008)
  • Author(s): K. Iwai, N. Misawa, and S. Takeuchi
  • Organizer: microTAS2008
• [Presentation] "Housing" for Cells in Monodisperse Microcages (2008)
  • Author(s): Yuya Morimoto, Wei-Heong Tan, Shoii Takeuchi
  • Organizer: MEMS2008
• [Presentation] A Resettable Dynamic Microfluidic Device (2008)
  • Author(s): Kosuke Iwai, Wei-Heong Tan, and Shoji Takeuchi
  • Organizer: MEMS2008
• [Presentation] Assembly of Single Adhesion Cells on Mobile Microplates (2008)
  • Author(s): Hiroaki Onoe, and Shoji Takeuchi
  • Organizer: MEMS2008
• [Presentation] Encapsulation of biomaterials in semi-permeable membrane (2008)
  • Author(s): A. Adachi, Y. Morimoto, Y. Tsuda and S. Takeuchi
  • Organizer: The 25th SENSOR SYMPOSIUM
• [Presentation] Formation of Monodisperse Microsized-Emulsions using an Axisymmetric Flow-Focuisng Device Fabricated by Photolithography and Stereolithography (2008)
  • Author(s): Y. Morimoto, K. Kaori and S. Takeuchi
  • Organizer: The 25th SENSOR SYMPOSIUM
• [Presentation] A Resettable Dynamic Micro Array (2008)
  • Author(s): Hirotaka Ishihara, Kosuke Iwai and Shoji Takeuchi
  • Organizer: Proceedings of The 25th Sensor Symposium
• [Presentation] Formation of Monodisperse Hydrogel Memrane Capsules for Cell Encapsulation (2008)
  • Author(s): Y. Morimoto and S. Takeuchi
  • Organizer: 第46回生物物理
• [Presentation] 均一直径半透膜マイクロカプセルの生化学実験応用 (2008)
  • Author(s): 安達亜希,森本雄矢,竹内昌治
  • Organizer: 生物物理第46回年会
• [Presentation] 大腸菌アレイ:ナノ流路を有するマイクロ流体デバイス (2008)
  • Author(s): 岩井孝介,三澤宣雄,竹内昌治
  • Organizer: 第46回日本生物物理学会年会
• [Presentation] マイクロプレートを用いた接着細胞のハンドリング (2008)
  • Author(s): 石原宏尚,栗林香織,竹内昌治
  • Organizer: 第46回日本生物物理学会
• [Presentation] 半透膜カプセルで生体材料を包む (2008)
  • Author(s): 安達亜希,森本雄矢,竹内昌治
  • Organizer: 「細胞を創る」研究会1.0
• [Presentation] 三次元ヘテロ組織化へむけた均一直径細胞ビーズの調製 (2008)
  • Author(s): 津田行子,森本雄矢,竹内昌治
  • Organizer: 日本バイオマテリアル学会シンポジウム
• [Presentation] Microfluidic Technology for Tissue Engineering (2008)
  • Author(s): Y. Tsuda, Y. Morimoto and S. Takeuchi
  • Organizer: The 15th international display workshops
• [Presentation] 溶液交換可能な半透膜マイクロカプセル (2008)
  • Author(s): 森本雄矢,竹内昌治
  • Organizer: 膜超分子モーターの革新的ナノサイエンス
• [Presentation] "Housing" for Cells in Monodisperse Microcages (2008)
  • Author(s): Yuya Morimoto, Wei-Heong Tan, Shoji Takeuchi
  • Organizer: MEMS2008
  • Place of Presentation: 米国アリゾナ
• [Presentation] A Resettable Dynamic Microfluidic Device (2008)
  • Author(s): osuke Iwai, Wei-Heong Tan, and Shoji Takeuchi
  • Organizer: MEMS2008
  • Place of Presentation: 米国アリゾナ
• [Presentation] Eukaryotic Flagella as Motile Tools for Microfluidic Devices (2007)
  • Author(s): Hajime Nakamura, Kaori Kuribayashi, Hiroaki Onoe and Shoji Takeuchi
  • Organizer: microTAS2007
• [Presentation] 細胞の網羅的解析のためのダイナミックマイクロアレイ (2007)
  • Author(s): Wei-Heong Tan, Yuya Morimoto and Shoji Takeuchi
  • Organizer: 「細胞を創る」研究会
• [Presentation] 3次元マイクロ流路を用いた微生物の均一径ハイドロゲルカプセル化 (2007)
  • Author(s): 森本雄矢,陳偉雄,竹内昌治
  • Organizer: 第45回日本生物物理学会年会
• [Presentation] 細胞解析の為のトラップ及びリリース機構を有するマイクロ流路デバイス(Trap-and-release Microfluidic Device For The Analysis of Cells) (2007)
  • Author(s): 岩井 孝介,Wei-Heong Tan,竹内 昌治
  • Organizer: 第45回日本生物物理学会年会
• [Presentation] Formation of Monodisperse Hydrogel Capsules using Three-Dimensional Microfluidic Channel (2007)
  • Author(s): Yuya Morimoto, Wei-Heiong Tan and Shoji Takeuchi
  • Organizer: 第24回「センサ・マイクロマシンと応用システム」シンポジウム
• [Book] 生体材料を利用したバイオハイブリッドプロセス, MEMS/NEMS工学全集 (2009)
  • Author(s): 竹内昌治
  • Publisher: テクノシステム
• [Remarks]
  • URL: http://www.hybrid.iis.u-tokyo.ac.jp/