Vibrational spectroscopy of single proteins
| Project/Area Number |
19685001
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Physical chemistry
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
FUJIYOSHI Satoru Tokyo Institute of Technology, 大学院・理工学研究科, 助教 (70371705)
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| Project Period (FY) |
2007 – 2011
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥24,830,000 (Direct Cost: ¥19,100,000、Indirect Cost: ¥5,730,000)
Fiscal Year 2010: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2008: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2007: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
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| Keywords | 分光 / 低温 / タンパク質 / 単一分子分光 / 赤外 / 中赤外 / アミドI / 単一分子 / 一分子計測(SMI) / 化学物理 / 光物性 / 物性実験 / 生物体分子 / 一分子計測(SDM) / 生体分子 |
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| Research Abstract |
Vibrational infrared absorption of a single protein molecule was detected at a few kelvins as infrared-induced recovery of visible fluorescence of a dye with which the protein was labeled. This sensitive method of detecting infrared absorption was demonstrated for a single bovine serum albumin (BSA) molecule labeled with Alexa Fluor 660 by determining the vibrational infrared absorption spectrum of the backbone vibrations of the R-helical structure in the wavelength region around 6μm (1650cm-1). In addition to measuring the vibrational infrared absorption spectrum, the visible fluorescence can be simultaneously used for imaging of the same dye-labeled single protein molecules.
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Report
(6 results)
Research Products
(61 results)
