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Vibrational spectroscopy of single proteins

Research Project

Project/Area Number 19685001
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Physical chemistry
Research InstitutionTokyo Institute of Technology

Principal Investigator

FUJIYOSHI Satoru  Tokyo Institute of Technology, 大学院・理工学研究科, 助教 (70371705)

Project Period (FY) 2007 – 2011
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥24,830,000 (Direct Cost: ¥19,100,000、Indirect Cost: ¥5,730,000)
Fiscal Year 2010: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2008: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2007: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Keywords分光 / 低温 / タンパク質 / 単一分子分光 / 赤外 / 中赤外 / アミドI / 単一分子 / 一分子計測(SMI) / 化学物理 / 光物性 / 物性実験 / 生物体分子 / 一分子計測(SDM) / 生体分子
Research Abstract

Vibrational infrared absorption of a single protein molecule was detected at a few kelvins as infrared-induced recovery of visible fluorescence of a dye with which the protein was labeled. This sensitive method of detecting infrared absorption was demonstrated for a single bovine serum albumin (BSA) molecule labeled with Alexa Fluor 660 by determining the vibrational infrared absorption spectrum of the backbone vibrations of the R-helical structure in the wavelength region around 6μm (1650cm-1). In addition to measuring the vibrational infrared absorption spectrum, the visible fluorescence can be simultaneously used for imaging of the same dye-labeled single protein molecules.

Report

(6 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report   Self-evaluation Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (61 results)

All 2011 2010 2009 2008 2007 Other

All Journal Article (16 results) (of which Peer Reviewed: 10 results) Presentation (45 results)

  • [Journal Article] Structural Change of a Cofactor Binding Site of Flavoprotein Detected by Single-Protein Fluorescence Spectroscopy at 1.5 K2011

    • Author(s)
      藤芳暁, その他
    • Journal Title

      Physical Review Letters

      Volume: 106

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Vibrational Microspectroscopy of Single Proteins2010

    • Author(s)
      S.Fujiyoshi, Y.Furuya, M.Iseki, M.Watanabe, M.Matsushita
    • Journal Title

      J.Phys.Chem.Lett 1号

      Pages: 2541-2545

    • Related Report
      2010 Final Research Report
  • [Journal Article] Vibrational Microscpectroscopy of Single Proteins2010

    • Author(s)
      藤芳暁, その他
    • Journal Title

      Journal of Physical Chemistry Letters

      Volume: 1 Pages: 2541-2545

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Single-Component reflecting objective for ultraviolet imaging and spectroscopy at cryogenic temperature2009

    • Author(s)
      M.Fujiwara, S.Fujiyoshi, M.Matsushita
    • Journal Title

      Journal of Optical Society of the America B 26

      Pages: 1395-1395

    • Related Report
      2010 Final Research Report
  • [Journal Article] Single-Component reflecting objective for ultraviolet imaging and spectroscopy at cryogenic temperature2009

    • Author(s)
      M. Fujiwara, S. Fujiyoshi, M. Matsushita
    • Journal Title

      Journal of Optical Society of the America B 26

      Pages: 1395-1395

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Single-component reflecting objective for ultraviolet imaging and spectroscopy at cryogenic temperature2009

    • Author(s)
      藤原・藤芳・松下
    • Journal Title

      Journal of Optical Society of Ameriaca B 26

      Pages: 1395-1399

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Visible Fluorescence Spectroscopy of Single Proteins at Helium-Liquid Temperatures2008

    • Author(s)
      S.Fujiyoshi, M.Fujiwara, M.Matsushita
    • Journal Title

      Physical Review Letters 100

    • Related Report
      2010 Final Research Report
  • [Journal Article] How deep is the potential well confining protein to a specific conformation? A single-molecule spectroscopy on temperature dependence of conformational change at 5-30 K2008

    • Author(s)
      H.Oikawa, S.Fujiyoshi, T.Dewa, M.Nango, M.Matsushita
    • Journal Title

      Journal of the American Chemical Society (Communication) 130

      Pages: 4580-4581

    • Related Report
      2010 Final Research Report
  • [Journal Article] Visible Fluorescence Spectroscopy of Single Proteins at Helium-Liquid Temperatures2008

    • Author(s)
      S. Fujiyoshi, M. Fujiwara, M. Matsushita
    • Journal Title

      Physical Review Letters 100

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] How deep is the potential well confining protein to a specific conformation? A single-molecule spectroscopy on temperature dependence of conformational change at 5 - 30 K2008

    • Author(s)
      H. Oikawa, S. Fujiyoshi, T. Dewa, M. Nango, M. Matsushita
    • Journal Title

      Journal of the American Chemical Society (Communication) 130

      Pages: 4580-4581

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Visible Fluorescence Spectroscopy of Single Proteins at Liquid-Helium Temperature2008

    • Author(s)
      Satoru Fujiyoshi,* Masanori Fujiwara, and Michio Matsushita
    • Journal Title

      PHYSICAL REVIEW LETTERS 100

      Pages: 168101-168101

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 四次および三次のラマン過程による界面振動分光2008

    • Author(s)
      藤芳 暁
    • Journal Title

      Molecular Science 2

      Pages: 184-184

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Single-Component Reflecting Objective for Low-Temperature Spectroscopy in the Entire Visible Region2007

    • Author(s)
      S.Fujiyoshi, T.Fujiwara, C.Kim, M.Matsushita, A.M.van Oijen, J.Schmidt
    • Journal Title

      Applied Physics Letters 91

    • Related Report
      2010 Final Research Report
  • [Journal Article] Single-Component Reflecting Objective for Low-Temperature Spectroscopy in the Entire Visible Region2007

    • Author(s)
      S. Fujiyoshi, T. Fujiwara, C. Kim, M. Matsushita, A.M. van Oijen, J. Schmidt
    • Journal Title

      Applied Physics Letters 91

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Single-component reflecting objective for low-tenperature spectroscopy in the entire visible region2007

    • Author(s)
      藤芳暁, 他五名
    • Journal Title

      Applied Physics Ltters 91

      Pages: 511253-511253

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural Change of a Cofactor Binding Site of Flavoprotein Detected by Single-Protein Fluorescence Spectroscopy at 1.5 K

    • Author(s)
      S.Fujiyoshi, M.Hirano, M.Matsushita, M.Iseki, M.Watanabe
    • Journal Title

      Physical Review Letters 106号

    • Related Report
      2010 Final Research Report
  • [Presentation] 光合成アンテナ複合体の中赤外スペクトルの一分子観測2011

    • Author(s)
      大友康平・出羽毅久・南保守・渡辺正勝・松下道雄・藤芳暁
    • Organizer
      日本化学会2011・4B4-11
    • Place of Presentation
      神奈川(講演ハイライトに選出)
    • Year and Date
      2011-03-29
    • Related Report
      2010 Final Research Report
  • [Presentation] 光合成アンテナ複合体の中赤外スペクトルの一分子観測2011

    • Author(s)
      大友康平, その他
    • Organizer
      日本化学会
    • Place of Presentation
      神奈川大学
    • Year and Date
      2011-03-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] タンパク質の赤外吸収の単一分子観測:色素の光熱サイクルに対する考察2010

    • Author(s)
      藤芳暁・古屋陽・伊関峰生・渡辺正勝・松下道雄
    • Organizer
      分子科学会2010・1B20
    • Place of Presentation
      大阪
    • Year and Date
      2010-09-14
    • Related Report
      2010 Final Research Report
  • [Presentation] タンパク質の赤外吸収の単一分子観測:色素の光熱サイクルに対する考察2010

    • Author(s)
      藤芳暁, その他
    • Organizer
      分子科学会
    • Place of Presentation
      大阪大学
    • Year and Date
      2010-09-14
    • Related Report
      2010 Annual Research Report
  • [Presentation] Single-component reflecting objective for single-protein spectroscopy at a few kelvins2010

    • Author(s)
      Satoru Fujiyoshi
    • Organizer
      GSC七夕ミーティング
    • Place of Presentation
      横浜
    • Year and Date
      2010-07-15
    • Related Report
      2010 Final Research Report
  • [Presentation] Single-componet reflecting objective for single-protein spectroscopy at a few kelvine2010

    • Author(s)
      Satoru Fujiyoshi
    • Organizer
      GSC七夕ミーティング
    • Place of Presentation
      横浜理研(招待講演)
    • Year and Date
      2010-07-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] 単一タンパク質の振動分光法の開発2010

    • Author(s)
      藤芳暁・古屋陽・伊関峰生・渡辺正勝・松下道雄(東工大物理・総研大)
    • Organizer
      日本化学会2010・2E1-39
    • Place of Presentation
      大阪
    • Year and Date
      2010-03-27
    • Related Report
      2010 Final Research Report
  • [Presentation] 単一タンパク質の振動分光法の開発2010

    • Author(s)
      藤芳暁・古屋陽・伊関峰生・渡辺正勝・松下道雄
    • Organizer
      日本化学会2010
    • Place of Presentation
      東工大物理・総研大
    • Year and Date
      2010-03-27
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] 単一タンパク質の振動分光法の開発2010

    • Author(s)
      藤芳・古屋・伊関・渡辺・松下
    • Organizer
      日本化学会
    • Place of Presentation
      近畿大学
    • Year and Date
      2010-03-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] 1.5K における単一色素分子の蛍光スペクトルの時間変化の起源探究2010

    • Author(s)
      島内明理・櫻井敦教・平野充遙・藤芳暁・松下道雄(東工大物理)
    • Organizer
      日本物理学会2010年春季大会・23aHL-3
    • Place of Presentation
      岡山
    • Year and Date
      2010-03-23
    • Related Report
      2010 Final Research Report
  • [Presentation] 1. 5Kにおける単一色素分子の蛍光スペクトルの時間変化の起源探究2010

    • Author(s)
      島内明理・櫻井敦教・平野充遙・藤芳暁・松下道雄
    • Organizer
      日本物理学会2010年春季大会
    • Place of Presentation
      東工大物理
    • Year and Date
      2010-03-23
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] 1.5Kにおける単一色素分子の蛍光スペクトルの時間変化の起源探究2010

    • Author(s)
      島内・櫻井・平野・藤芳・松下
    • Organizer
      日本物理学会
    • Place of Presentation
      岡山大学
    • Year and Date
      2010-03-23
    • Related Report
      2009 Annual Research Report
  • [Presentation] 低温固体中の単一Pr3+核スピン量子状態分光の試み2010

    • Author(s)
      中村一平・吉弘達矢・藤芳暁・松下道雄(東工大物理)
    • Organizer
      物理学会2010年春季大会・21pTD-5
    • Place of Presentation
      岡山
    • Year and Date
      2010-03-21
    • Related Report
      2010 Final Research Report
  • [Presentation] 低温固体中の単一Pr3+核スピン量子状態分光の試み2010

    • Author(s)
      中村一平・吉弘達矢・藤芳暁・松下道雄
    • Organizer
      物理学会2010年春季大会
    • Place of Presentation
      東工大物理
    • Year and Date
      2010-03-21
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] 光活性化アデニル酸シクラーゼの単一タンパク質分光2009

    • Author(s)
      藤芳暁・平野充遙・松下道雄・伊関峰生・渡辺正勝(東工大物理・総研大)
    • Organizer
      分子科学討論会2009・4B07
    • Place of Presentation
      名古屋
    • Year and Date
      2009-09-24
    • Related Report
      2010 Final Research Report
  • [Presentation] 低温下における単一タンパク質分光のための反射対物レンズの開発2009

    • Author(s)
      藤原正規・藤芳暁・松下道雄(東工大物理)
    • Organizer
      分子科学討論会2009・4B06
    • Place of Presentation
      名古屋
    • Year and Date
      2009-09-24
    • Related Report
      2010 Final Research Report
  • [Presentation] 光活性化アデニル酸シクラーゼの単一タンパク質分光2009

    • Author(s)
      藤芳暁・平野充遙・松下道雄・伊関峰生・渡辺正勝
    • Organizer
      分子科学討論会2009
    • Place of Presentation
      東工大物理・総研大
    • Year and Date
      2009-09-24
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] 低温下における単一タンパク質分光のための反射対物レンズの開発2009

    • Author(s)
      藤原正規・藤芳暁・松下道雄
    • Organizer
      分子科学討論会2009
    • Place of Presentation
      東工大物理
    • Year and Date
      2009-09-24
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] 低温下における単一タンパク質分光のための反射対物レンズの開発2009

    • Author(s)
      藤原・藤芳・松下
    • Organizer
      分子科学討論会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2009-09-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] 光活性化アデニル酸シクラーゼの単一タンパク質分光2009

    • Author(s)
      藤芳・平野・松下・伊関・渡辺
    • Organizer
      分子科学討論会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2009-09-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] 液体ヘリウム温度における単一タンパク質の可視蛍光分光2009

    • Author(s)
      藤芳暁(東工大物理)
    • Organizer
      日本物理学会2009年春季大会(若手奨励賞受賞講演)28pVB-2
    • Place of Presentation
      東京
    • Year and Date
      2009-03-28
    • Related Report
      2010 Final Research Report
  • [Presentation] 液体ヘリウム温度における単一タンパク質の可視蛍光分光2009

    • Author(s)
      藤芳暁
    • Organizer
      日本物理学会2009年春季大会
    • Place of Presentation
      東工大物理
    • Year and Date
      2009-03-28
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] 低温固体中の単一Pr3+の発光検出の試み2009

    • Author(s)
      吉弘達矢・中村一平・藤芳暁・松下道雄(東工大物理・さきがけ)
    • Organizer
      日本物理学会2009年春季大会・27pSK-9
    • Place of Presentation
      東京
    • Year and Date
      2009-03-27
    • Related Report
      2010 Final Research Report
  • [Presentation] 低温固体中の単一Pr3+の発光検出の試み2009

    • Author(s)
      吉弘達矢・中村一平・藤芳暁・松下道雄
    • Organizer
      日本物理学会2009年春季大会
    • Place of Presentation
      東工大物理・さきがけ
    • Year and Date
      2009-03-27
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] 低温の単一タンパク質分光の励起過程の検討2008

    • Author(s)
      藤芳暁・平野充遥・古屋陽・藤原正規・松下道雄・伊関峰生・渡辺正勝(東工大物理・総研大)
    • Organizer
      第2回分子科学討論会・3C18
    • Place of Presentation
      福岡
    • Year and Date
      2008-09-26
    • Related Report
      2010 Final Research Report
  • [Presentation] 低温の単一タンパク質分光の励起過程の検討2008

    • Author(s)
      藤芳暁・平野充遥・古屋陽・藤原正規・松下道雄・伊関峰生・渡辺正勝
    • Organizer
      第2回分子科学討論会
    • Place of Presentation
      東工大物理・総研大
    • Year and Date
      2008-09-26
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] 低温の単一タンパク質分光の励起過程の検討2008

    • Author(s)
      藤芳暁、平野充遥、古屋陽、藤原正規、松下道雄、伊関峰生、渡辺正勝
    • Organizer
      分子科学討論会
    • Place of Presentation
      九州
    • Year and Date
      2008-09-26
    • Related Report
      2008 Annual Research Report
  • [Presentation] タンパク質の生体機能の単一分子観測に向けて2008

    • Author(s)
      藤芳暁(東工大物理)
    • Organizer
      日本物理学会2008年秋季大会(シンポジウム講演)・22pWB-8
    • Place of Presentation
      岩手
    • Year and Date
      2008-09-22
    • Related Report
      2010 Final Research Report
  • [Presentation] タンパク質の生体機能の単一分子観測に向けて2008

    • Author(s)
      藤芳暁
    • Organizer
      日本物理学会2008年秋季大会
    • Place of Presentation
      東工大物理
    • Year and Date
      2008-09-22
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] 低温固体中の単一イオンの発光検出に向けた可視顕微分光システムの開発2008

    • Author(s)
      吉弘達矢・平野充遙・藤芳暁・松下道雄
    • Organizer
      日本物理学会2008年秋季大会・20pZA-5
    • Place of Presentation
      岩手
    • Year and Date
      2008-09-20
    • Related Report
      2010 Final Research Report
  • [Presentation] 低温固体中の単一イオンの発光検出に向けた可視顕微分光システムの開発2008

    • Author(s)
      吉弘達矢・平野充遙・藤芳暁・松下道雄
    • Organizer
      日本物理学会2008年秋季大会
    • Year and Date
      2008-09-20
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] 二光子蛍光による低温の単一タンパク質分光2008

    • Author(s)
      藤芳暁・松下道雄
    • Organizer
      日本物理学会2008年春季大会・23pYD-6
    • Place of Presentation
      大阪
    • Year and Date
      2008-03-23
    • Related Report
      2010 Final Research Report
  • [Presentation] 1.5Kで使える反射対物レンズの球面収差及び色収差の改善2008

    • Author(s)
      藤原正規・藤芳暁・松下道雄
    • Organizer
      日本物理学会2008年春季大会・23pYD-5
    • Place of Presentation
      大阪
    • Year and Date
      2008-03-23
    • Related Report
      2010 Final Research Report
  • [Presentation] 顕微分光による光合成アンテナ複合体のエネルギー移動の研究2008

    • Author(s)
      古屋陽・内山大輔・藤芳暁・松下道雄・末守良春・出羽毅久・南後守
    • Organizer
      日本物理学会2008年春季大会・23pYD-4
    • Place of Presentation
      大阪
    • Year and Date
      2008-03-23
    • Related Report
      2010 Final Research Report
  • [Presentation] 二光子蛍光による低温の単一タンパク質分光2008

    • Author(s)
      藤芳暁・松下道雄
    • Organizer
      日本物理学会2008年春季大会
    • Year and Date
      2008-03-23
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] 1. 5 Kで使える反射対物レンズの球面収差及び色収差の改善2008

    • Author(s)
      藤原正規・藤芳暁・松下道雄
    • Organizer
      日本物理学会2008年春季大会
    • Year and Date
      2008-03-23
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] 顕微分光による光合成アンテナ複合体のエネルギー移動の研究2008

    • Author(s)
      古屋陽・内山大輔・藤芳暁・松下道雄・末守良春・出羽毅久・南後守
    • Organizer
      日本物理学会2008年春季大会
    • Year and Date
      2008-03-23
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] 二光子蛍光による低温の単一タンパク質分光2008

    • Author(s)
      藤芳暁・松下道雄
    • Organizer
      物理学会
    • Place of Presentation
      近畿大学
    • Year and Date
      2008-03-23
    • Related Report
      2007 Annual Research Report
  • [Presentation] 1.5Kで使える反射対物レンズの球面収差及び色収差の改善2008

    • Author(s)
      藤原正規, 藤芳暁, 松下道雄
    • Organizer
      物理学会
    • Place of Presentation
      近畿大学
    • Year and Date
      2008-03-23
    • Related Report
      2007 Annual Research Report
  • [Presentation] 顕微分光による光合成アンテナ複合体間のエネルギー移動の研究2008

    • Author(s)
      古屋陽, 内山大輔, 藤芳暁, 松下道雄, 末守好春, 出羽毅久, 南後守
    • Organizer
      物理学会
    • Place of Presentation
      近畿大学
    • Year and Date
      2008-03-23
    • Related Report
      2007 Annual Research Report
  • [Presentation] 可視蛍光をプローブとした低温の単一タンパク質分光2007

    • Author(s)
      藤芳暁・藤原正規・松下道雄
    • Organizer
      第1回分子科学討論会・2C11
    • Place of Presentation
      宮城
    • Year and Date
      2007-09-21
    • Related Report
      2010 Final Research Report
  • [Presentation] 人工脂質膜中における光合成アンテナ複合体の会合体の分光2007

    • Author(s)
      内山大輔・古屋陽・藤芳暁・松下道雄・出羽毅久・南後守
    • Organizer
      日本物理学会2007年秋季大会・21aRK-3
    • Place of Presentation
      北海道
    • Year and Date
      2007-09-21
    • Related Report
      2010 Final Research Report
  • [Presentation] 可視蛍光をプローブとした低温の単一タンパク質分光2007

    • Author(s)
      藤芳暁・藤原正規・松下道雄
    • Organizer
      第1回分子科学討論会
    • Year and Date
      2007-09-21
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] 人工脂質膜中における光合成アンテナ複合体の会合体の分光2007

    • Author(s)
      内山大輔・古屋陽・藤芳暁・松下道雄・出羽毅久・南後守
    • Organizer
      日本物理学会2007年秋季大会
    • Year and Date
      2007-09-21
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] 可視蛍光をプローブとした低温の単一タンパク質分光2007

    • Author(s)
      藤芳暁・藤原正規・松下道雄
    • Organizer
      分子科学討論会
    • Place of Presentation
      東北大学
    • Year and Date
      2007-09-18
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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