Rational Design of Unnatural Substrates for Expanded Ribosomal Synthesis of Sequence-Regulated Compounds

• Project/Area Number: 19685016
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Chemistry related to living body
• Research Institution: Kyoto University
• Principal Investigator: SANDO Shinsuke Kyoto University, 稲盛フロンティア研究センター, 教授 (20346084)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥25,090,000 (Direct Cost: ¥19,300,000、Indirect Cost: ¥5,790,000); Fiscal Year 2009: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000); Fiscal Year 2008: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2007: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
• Keywords: 蛋白質 / リボソーム / 配列制御型合成 / 配列制御合成 / 核酸 / たんぱく質 / アミノ酸

Research Abstract

The cellular translation system is a sophisticated molecular system that can polymerize substrates (amino acids) in a highly sequence-programmed manner to produce biomolecules (proteins). In this research, we challenged expansion of substrates that can be used for ribosomal-synthesis of a variety of artificial compounds, especially by chemical approach, and developed a new method for efficient use of such unnatural substrates.

Research Products

• [Journal Article] Synthesis of puromycin derivatives with backbone-elongated substrates and associated translation inhibitory activities. (2009)
  • Author(s): Mizusawa Keigo, Abe Kenji, Shinsuke Sando, Yasuhiro Aoyama
  • Journal Title: Bioorg. Med. Chem. 17 Pages: 2381-2387
• [Journal Article] Synthesis of puromycin derivatives with backbone-elongated substrates and associated translation (2009)
  • Author(s): Keigo Mizusawa, Kenji Abe, Shinsuke Sando, and Yasuhiro
  • Journal Title: Bloom. Med. Chem. 17 Pages: 2381-2387
  • Peer Reviewed
• [Journal Article] Enzymatic N-methylaminoacylation of tRNA using chemically misacylated AMP as a substrate. (2008)
  • Author(s): Sando Shinsuke, Masu Hiroki, Furutani Chika, Aoyama Yasuhiro
  • Journal Title: Org. Biomol. Chem. 6 Pages: 2666-2668
• [Journal Article] Enzymatic N-methylaminoacylation of tRNA using chemically misacylated AMP as a substrate (2008)
  • Author(s): Shinsuke Sando, Hiroki Masu, Chika Furutani and Yasuhiro Aovama
  • Journal Title: Org. Biomol. Chem. 6 Pages: 2666-2668
  • Peer Reviewed
• [Presentation] 日本化学会進歩賞受賞講演:"生体分析を目指した機能性核酸システムの創製" (2009)
  • Author(s): 東信介
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 日本大学
• [Presentation] リボソームを用いた非天然型高分子合成に向けたアプローチ:化学的ミスアシル化AMP法に基づく非天然基質の触媒的tRNA付加反応と翻訳系への展開 (2008)
  • Author(s): 山東信介、益啓貴、青山安宏
  • Organizer: 日本化学会第88春季年会(2008)
  • Place of Presentation: 立教大学
  • Year and Date: 2008-03-29
• [Presentation] リボソームを用いた非天然型高分子合成に向けたアプローチ:化学的ミスアシル化AMP法に基づく非天然基質の触媒的tRNA付加反応と翻訳系への展開 (2008)
  • Author(s): 山東信介、益啓貴、青山安宏
  • Organizer: 日本化学会第88春季年会
  • Place of Presentation: 立教大学
• [Presentation] 翻訳システムを用いた非天然型高分子合成に向けた化学的アプローチ:Chemicaly Misacylated'AMP法による非天然型基質の触媒的tRNAミスアシル化 (2007)
  • Author(s): 山東信介、益啓貴、青山安宏
  • Organizer: 第2回 無細胞生命科学研究会
  • Place of Presentation: 東京大学
  • Year and Date: 2007-10-19
• [Presentation] 翻訳システムを用いた非天然型高分子合成に向けた化学的アプローチ: Chemicaly Misacylated AMP法による非天然型基質の触媒的tRNAミスアシル化 (2007)
  • Author(s): 山東信介、益啓貴、青山安宏
  • Organizer: 第2回無細胞生命科学研究会
  • Place of Presentation: 東京大学
• [Remarks]
  • URL: http://www.inamori-frontier.kyushu-u.ac.jp/soft_material/