Multi-scale biomanipulation using magnetic nanoparticles

• Project/Area Number: 19686049
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Biofunction/Bioprocess
• Research Institution: Kyushu University
• Principal Investigator: ITO Akira Kyushu University, 大学院・工学研究院, 准教授 (60345915)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000); Fiscal Year 2008: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000); Fiscal Year 2007: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
• Keywords: ティッシュエンジニアリング / 磁性粒子 / 再生医療 / ナノバイオ / 磁性ナノ粒子 / リポソーム / 磁気分離 / 遺伝子導入 / 共培養 / マグネタイト / パターニング

Research Abstract

10nm程の直径の磁性ナノ粒子を、様々なバイオマテリアルで修飾することで、ナノバイオ磁性ナノ粒子を開発し、磁力を用いて細胞を操作する技術を開発した。具体的には、(1)レトロウイルスベクターを磁気操作する技術、(2)共培養後の標的細胞を磁気分離する技術、(3)細胞を磁力で配置するパターニング技術、(4)細胞を磁力で集積させて高度な三次元組織を構築する技術、といったナノからミリメーターに及ぶマルチスケールバイオマニピュレーションの開発を行った。

Research Products

• [Journal Article] Cell-patterning using poly (ethylene glycol)-modified magnetite nanoparticles (2009)
  • Author(s): Akiyama H, Ito A, Kawabe Y, Kamihira M
  • Journal Title: J Biomed Mater Res A (in press)
  • Peer Reviewed
• [Journal Article] Fabrication of complex three-dimensional tissue architectures using a magnetic force-based cell patterning technique (2009)
  • Author(s): Akiyama H, Ito A, Kawabe Y, Kamihira M
  • Journal Title: Biomed Microdevices (in press)
  • Peer Reviewed
• [Journal Article] Magnetic separation of cells in coculture systems using magnetite cationic liposomes (2009)
  • Author(s): Ito A, Jitsunobu H, Kawabe Y, Ijima H, Kamihira M
  • Journal Title: Tissue Eng Part C Methods (in press)
  • Peer Reviewed
• [Journal Article] Magnetic concentration of a retroviral vector using magnetite cationic liposomes (2009)
  • Author(s): Ito A, Takahashi T, Kameyama Y, Kawabe Y, Kamihira M
  • Journal Title: Tissue Eng Part C Methods 15 Pages: 57-64
  • Peer Reviewed
• [Journal Article] Fabrication of complex three-dimensional tissue architectures using a magnetic force-based cell patterning technique (2009)
  • Author(s): Akiyama H, et.al.
  • Journal Title: Biomedical Microdevices 11(in press)
  • Peer Reviewed
• [Journal Article] Magnetic separation of cells in co-culture systems using magnetite cationic liposomes (2009)
  • Author(s): Ito A, et.al.
  • Journal Title: Tissue Engineering Part C : Methods 15(in press)
  • Peer Reviewed
• [Journal Article] Magnetic concentration of a retroviral vector using magnetite cationic liposomes (2009)
  • Author(s): Ito A, et.al.
  • Journal Title: Tissue Engineering Part C : Methods 15(in press)
  • Peer Reviewed
• [Journal Article] Cell-pattering using poly (ethylene glycol) -modified magnetite nanoparticles (2009)
  • Author(s): Akiyama H, et.al.
  • Journal Title: Journal of Biomedical Materials Research : Part A (in press)
  • Peer Reviewed
• [Journal Article] Enhancement of cell function through heterotypic cell-cell interactions using E-cadherin-expressing NIH3T3 cells (2008)
  • Author(s): Ito A, Kiyohara T, Kawabe Y, Ijima H, Kamihira M
  • Journal Title: J Biosci Bioeng 105 Pages: 679-682
  • NAID: 110006792548
  • Peer Reviewed
• [Journal Article] Enhancement of cell function through heterotypic cell-cell interactions using E-cadherin-expressing NIH3T3 cells (2008)
  • Author(s): Ito A, et.al.
  • Journal Title: Journal of Bioscience and Bioengineering 105 Pages: 679-682
  • NAID: 110006792548
  • Peer Reviewed
• [Journal Article] Construction of 3D tissue-like structure using functional magnetite nanoparticles (2008)
  • Author(s): Ito, A, et. al.
  • Journal Title: Yakugaku Zasshi 128 Pages: 21-28
  • NAID: 110006532999
  • Peer Reviewed
• [Journal Article] Construction of heterotypic cell sheets by magnetic force-based 3-D coculture of HepG2 and NIH3T3 cells (2007)
  • Author(s): Ito A, Jitsunobu H, Kawabe Y, Kamihira M
  • Journal Title: J Biosci Bioeng 104 Pages: 371-378
  • NAID: 110006402606
  • Peer Reviewed
• [Journal Article] Magnetic force-based cell patterning using Arg-Gly-Asp (RGD) peptide-conjugated magnetite cationic liposomes (2007)
  • Author(s): Ito A, Akiyama H, Kawabe Y, Kamihira M
  • Journal Title: J Biosci Bioeng 104 Pages: 288-293
  • NAID: 110006437009
  • Peer Reviewed
• [Journal Article] Construction of heterotypic cell sheets by magnetic force-based 3-D coculture of HepG2 and NIH 3T3 cells. (2007)
  • Author(s): Ito, A, et. al.
  • Journal Title: J. Biosci. Bioeng. 104 Pages: 371-378
  • NAID: 110006402606
  • Peer Reviewed
• [Journal Article] Mangnetic force-based cell patterning using Arg-Gly-Asp(RGD) peptide-conjugated magnetite cationic liposomes. (2007)
  • Author(s): Ito, A, et. al.
  • Journal Title: J. Biosci. Bioeng. 104 Pages: 288-293
  • Peer Reviewed
• [Presentation] マグネタイトカチオニックリポソームを用いた共培養系からの細胞の磁気分離法の開発 (2009)
  • Author(s): 井藤彰 他
  • Organizer: 化学工学会第74年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-03-20
• [Presentation] Ehancement of hepatocyte function through heterotypic cell-cell interactions using E-cadherin-expressing NIH3T3 cells (2008)
  • Author(s): Ito A, et al
  • Organizer: the 20th JAACT meeting
  • Place of Presentation: Fukuoka
  • Year and Date: 2008-11-26
• [Presentation] Ehancement of hepatocyte function through heterotypic cell-cell interactions using E-cadherin-expressing NIH3T3 cells (2008)
  • Author(s): Ito A, et.al.
  • Organizer: the 20^<th> JAACT meeting
  • Place of Presentation: Fukuoka
  • Year and Date: 2008-11-26
• [Presentation] Magnetic manipulation of a retroviral vector using magnetite cationic liposomes (2008)
  • Author(s): Ito A, et al
  • Organizer: MHS2008
  • Place of Presentation: MHS2008
  • Year and Date: 2008-11-08
• [Presentation] Magnetic manipulation of a retroviral vector using magnetite cationic liposomes (2008)
  • Author(s): Ito A, et.al.
  • Organizer: MHS2008
  • Place of Presentation: Nagoya
  • Year and Date: 2008-11-08
• [Presentation] 機能性磁性ナノ粒子を用いたティッシュエンジニアリング技術の開発 (2008)
  • Author(s): 井藤彰
  • Organizer: 第73回化学工学会年会
  • Place of Presentation: 浜松
  • Year and Date: 2008-03-19
• [Presentation] 機能性磁性ナノ粒子を用いたティッシュエンジニアリング技術の開発 (2008)
  • Author(s): 井藤 彰
  • Organizer: 第73回化学工学会年会
  • Place of Presentation: 浜松市
  • Year and Date: 2008-03-19
• [Presentation] 磁力を用いた三次元組織構築法の開発 (2008)
  • Author(s): 井藤彰 他
  • Organizer: 第7回日本再生医療学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-03-13
• [Presentation] 磁力を用いた三次元組織構築法の開発 (2008)
  • Author(s): 井藤 彰、本多裕之、上平正道
  • Organizer: 第7回日本再生医療学会総会
  • Place of Presentation: 名古屋市
  • Year and Date: 2008-03-13
• [Remarks]
  • URL: http://hyoka.ofc.kyushu-u.ac.jp/search/details/K002906/index.html
• [Remarks]
  • URL: http://hyoka.ofc.kyushu-u.ac.jp/search/details/K002906/index.html
• [Remarks]
  • URL: http://hyoka.ofc.kyushu-u.ac.jp/search/details/k002906/index.html