• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of the protein-turnover mechanisms in the chloroplast

Research Project

Project/Area Number 19687003
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field 植物生理・分子
Research InstitutionHokkaido University

Principal Investigator

TANAKA Ryouichi  Hokkaido University, 低温科学研究所, 准教授 (20311516)

Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥12,090,000 (Direct Cost: ¥9,300,000、Indirect Cost: ¥2,790,000)
Fiscal Year 2009: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2007: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Keywords葉緑体 / プロテアーゼ / シロイヌナズナ
Research Abstract

We found that a specific sequence in a chloroplast protein, chlorophyllide a oxygenase, is essential in the turnover of this protein. This sequence is most likely a degron for the chloroplast Clp protease. We obtained evidence for the presence of similar degrons in the other chloroplast proteins, indicating that the protein turnover mechanism is rather general in the chloroplast.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (51 results)

All 2010 2009 2008 2007 Other

All Journal Article (30 results) (of which Peer Reviewed: 28 results) Presentation (18 results) Remarks (3 results)

  • [Journal Article] LIL3, a light-harvesting-like protein, plays an essential role in chlorophyll and tocopherol biosynthesis.2010

    • Author(s)
      Tanaka R, Rothbart M, Oka S, Takabayashi A, Takahashi K, Shibata M, Myouga F, Motohashi R, Shinozaki K, Grimm B, Tanaka A
    • Journal Title

      Proc Natl Acad Sci USA 107

      Pages: 16721-16725

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a gene essential for protoporphyrinogen IX oxidase activity in the cyanobacterium Synechocystis sp. PCC6803.2010

    • Author(s)
      Kato K, Tanaka R, Sano S, Tanaka A, Hosaka H
    • Journal Title

      Proc Natl Acad Sci USA 107

      Pages: 16649-16654

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Deregulated chlorophyll b synthesis reduces the energy transfer rate between photosynthetic pigments and induces photodamage in Arabidopsis thaliana.2010

    • Author(s)
      Sakuraba Y, Yokono M, Akimoto S, Tanaka R, Tanaka A
    • Journal Title

      Plant Cell Physiol 51

      Pages: 1055-1065

    • NAID

      10027350305

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Microbial community structure, pigment composition, and nitrogen source of red snow in Antarctica.2010

    • Author(s)
      Fujii M, Takano Y, Kojima H, Hoshino T, Tanaka R, Fukui M
    • Journal Title

      Microb Ecol 59

      Pages: 466-475

    • NAID

      120003837195

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of AtNAP1 in the regulation of chlorophyll degradation in Arabidopsis thaliana2010

    • Author(s)
      Nagane T, Tanaka A, Tanaka R
    • Journal Title

      Planta 231

      Pages: 939-949

    • NAID

      120002834672

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] LIL3, a light-harvesting-like protein, plays an essential role in chlorophyll and tocopherol biosynthesis2010

    • Author(s)
      Ryouichi Tanaka
    • Journal Title

      Proc Natl Acad Sci U S A

      Volume: 107 Pages: 16721-16725

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a gene essential for protoporphyrinogen IX oxidase activity in the cyanobacterium Synechocystis sp.PCC68032010

    • Author(s)
      Kazushige Kato
    • Journal Title

      Proc Natl Acad Sci U S A

      Volume: 107 Pages: 16649-16654

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The cell biology of tetrapyrroles : a life and death struggle2010

    • Author(s)
      Nobuyoshi Mochizuki
    • Journal Title

      Trends Plant Sci

      Volume: 15 Pages: 488-498

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Regulation and Functions of the Chlorophyll Cycle2010

    • Author(s)
      Ryouichi Tanaka
    • Journal Title

      The Chloroplast : Basics and Applications

      Volume: 1 Pages: 55-77

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Deregulated chlorophyll b synthesis reduces the energy transfer rate between photosynthetic pigments and induces photodamage in Arabidopsis thaliana.2010

    • Author(s)
      Yasuhito Sakuraba
    • Journal Title

      Plant Cell Physiol

      Volume: 51 Pages: 1055-1065

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microbial Community Structure, Pigment Composition, and Nitrogen Source of Red Snow in Antarctica.2010

    • Author(s)
      Masanori Fujii
    • Journal Title

      Microb Ecol

      Volume: 59 Pages: 466-475

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of AtNAP1 in the regulation of chlorophyll degradation in Arabidopsis thaliana.2010

    • Author(s)
      Tomohiro Nagane
    • Journal Title

      Planta

      Volume: 231 Pages: 939-949

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Determination of a chloroplast degron in the regulatory domain of chlorophyllide a oxygenase.2009

    • Author(s)
      Sakuraba Y, Tanaka R, Yamasato A, Tanaka A.
    • Journal Title

      J Biol Chem 284

      Pages: 36689-36699

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Participation of chlorophyll b reductase in the initial step of the degradation of light-harvesting chlorophyll a/b-protein complexes in Arabidopsis.2009

    • Author(s)
      Horie Y, Ito H, Kusaba M, Tanaka R, Tanaka A
    • Journal Title

      J.Biol.Chem. 284

      Pages: 17449-17456

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Fractionation of hydrogen isotopes during phytol biosynthesis2009

    • Author(s)
      Chikaraishi Y, Tanaka R, Tanaka A, Ohkouchi N
    • Journal Title

      Organic Geochemistry 40

      Pages: 569-573

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Light-independent cell death induced by accumulation of pheophorbide a in Arabidopsis thaliana.2009

    • Author(s)
      Hirashima M, Tanaka R, Tanaka A
    • Journal Title

      Plant Cell Physiol 50

      Pages: 719-729

    • NAID

      10027339208

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] The steady-state level of Mg-protoporphyrin IX is not a determinant of plastid-to-nucleus signaling in Arabidopsis.2008

    • Author(s)
      Mochizuki N, Tanaka R, Tanaka A, Masuda T, Nagatani A
    • Journal Title

      Proc Natl Acad Sci USA 105

      Pages: 15184-15189

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Loss of the N-terminal domain of chlorophyllide a oxygenase induces photodamage during greening of Arabidopsis seedlings2008

    • Author(s)
      Yamasato A, Tanaka R, Tanaka A
    • Journal Title

      BMC Plant Biology 8

      Pages: 64-64

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of Arabidopsis mutants defective in the regulation of chlorophyllide a oxygenase2008

    • Author(s)
      Kanematsu S, Sakuraba Y, Tanaka A, Tanaka R
    • Journal Title

      Photochem Photobiol Sci 7

      Pages: 1196-1205

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a novel vinyl reductase gene essential for the biosynthesis of monovinyl chlorophyll in Synechocystis sp.2008

    • Author(s)
      Ito H, Yokono M, Tanaka R Tanaka A
    • Journal Title

      PCC6803 J Biol Chem 283

      Pages: 9002-9011

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of Arabidopsis Mutants Defective in the Regulation of Chlorophyllide a Oxygenase2008

    • Author(s)
      Kanematsu S., Sakuraba Y., Tanaka A., Tanaka R.
    • Journal Title

      Photochem Photobiol Sci 7

      Pages: 1196-1205

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Loss of the N-terminal domain of chlorophyllide a oxygenase induces photodamage during greening of Arabidopsis seedlings2008

    • Author(s)
      Yamasato A, Tanaka R, Tanaka A
    • Journal Title

      BMC Plant Biology 8(Article No.64)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The steady-state level of Mg-protoporphyrin IX is not a determinant of plastid-to-nucleus signaling in Arabidopsis2008

    • Author(s)
      Mochizuki N, Tanaka R, Tanaka A, Masuda T and Nagatani A
    • Journal Title

      Proc Nail Acad Sci USA 105

      Pages: 15184-15189

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] クロロフィル代謝2008

    • Author(s)
      田中歩, 田中亮一
    • Journal Title

      植物の生長調節 43

      Pages: 95-105

    • NAID

      110007007933

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a novel vinyl reductase gene essential for the biosynthesis of monovinyl chlorophyll in synechocystis sp. PCC6803.2008

    • Author(s)
      Ito H., Yokono M., Tanaka R. and Tanaka A.
    • Journal Title

      J. Biol. Chem. 283

      Pages: 9002-9011

    • NAID

      120000952725

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The major route for chlorophyll synthesis includes [3,8-divinyl]-chlorophyllide a reduction in Arabidopsis thaliana.2007

    • Author(s)
      Nagata N, Tanaka R, Tanaka A
    • Journal Title

      Plant Cell Physiol 48

      Pages: 1803-1808

    • NAID

      10027006454

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional analysis of N-terminal domains of Arabidopsis chlorophyllide a oxygenase2007

    • Author(s)
      Sakuraba Y, Yamasato A., Tanaka R, Tanaka A
    • Journal Title

      Plant Physiol Biochem 45

      Pages: 740-749

    • NAID

      120000952726

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional analysis of N-terminal domains of Arabidopsis chlorophyllide a oxygenase.2007

    • Author(s)
      櫻庭康仁、山里明弘、田中亮一、田中歩
    • Journal Title

      Plant Physiol Biochem 45

      Pages: 740-749

    • NAID

      120000952726

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tetrapyrrole Biosynthesis in Higher Plants.2007

    • Author(s)
      Tanaka, R. and Tanaka, A.
    • Journal Title

      Annu. Rev. Plant Biol. 58

      Pages: 321-346

    • Related Report
      2007 Annual Research Report
  • [Journal Article] The Major Route for Chlorophyll Synthesis Includes [3,8-divinyl]-chlorophyllide a Reduction in Arabidopsis thaliana.2007

    • Author(s)
      Nagata N., Tanaka R. and Tanaka A.
    • Journal Title

      Plant Cell Physiol 48

      Pages: 1803-1808

    • NAID

      10027006454

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Light-harvesting-like proteins : Functions and evolution2010

    • Author(s)
      Ryouichi Tanaka
    • Organizer
      XIII Seoul National University-Hokkaido University Joint Symposium
    • Place of Presentation
      札幌市、北海道大学
    • Year and Date
      2010-11-26
    • Related Report
      2009 Final Research Report
  • [Presentation] Light-harvesting-like proteins : Functions and evolution2010

    • Author(s)
      Ryouichi Tanaka
    • Organizer
      XIII Seoul National University-Hokkaido University Joint Symposium
    • Place of Presentation
      札幌市、北海道大学(招待講演)
    • Year and Date
      2010-11-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] クロロフィル合成およびトコフェロール合成に関与する,LHCモチーフを持つタンパク質の同定2010

    • Author(s)
      田中亮一
    • Organizer
      第51回日本植物生理学会
    • Place of Presentation
      熊本市、熊本大学
    • Year and Date
      2010-03-18
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] D1タンパク質の改変によるジビニルクロロフィルへの適合性の獲得2010

    • Author(s)
      伊藤寿,田中亮一,田中歩
    • Organizer
      第51回日本植物生理学会
    • Place of Presentation
      熊本市、熊本大学
    • Year and Date
      2010-03-18
    • Related Report
      2009 Final Research Report
  • [Presentation] シロイヌナズナの種子形成におけるクロロフィルb還元酵素の機能解析2010

    • Author(s)
      中島沙織,伊藤寿,田中亮一,田中歩
    • Organizer
      第51回日本植物生理学会
    • Place of Presentation
      熊本市、熊本大学
    • Year and Date
      2010-03-18
    • Related Report
      2009 Final Research Report
  • [Presentation] クロロフィル分解に関与するフラビンタンパク質2010

    • Author(s)
      目黒美生, 伊藤寿, 高林厚史, 田中亮一, 田中歩
    • Organizer
      第51回日本植物生理学会
    • Place of Presentation
      熊本市、熊本大学
    • Year and Date
      2010-03-18
    • Related Report
      2009 Final Research Report
  • [Presentation] シロイヌナズナのクロロフィルb還元酵素の生理機能解析2009

    • Author(s)
      中島沙織、伊藤寿、草場信、田中亮一、田中歩
    • Organizer
      第50回日本植物生理学会年会
    • Place of Presentation
      名古屋大学東山キャンパス、名古屋市
    • Year and Date
      2009-03-23
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] クロロフィル分解の中間体を蓄積する変異株の解析2009

    • Author(s)
      長谷川博之, 伊藤寿, 田中亮一, 田中歩
    • Organizer
      第50回日本植物生理学会年会
    • Place of Presentation
      名古屋大学東山キャンパス、名古屋市
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] ラン藻Synechocystis sp.PCC 6803由来プロトポルフィリノーゲンIXオキシダーゼ遺伝子の探索2008

    • Author(s)
      加登一成, 田中亮一, 田中歩, 保坂秀夫
    • Organizer
      第49回日本植物生理学会年会
    • Place of Presentation
      札幌コンベンションセンター、札幌市
    • Year and Date
      2008-03-22
    • Related Report
      2009 Final Research Report
  • [Presentation] クロロフィリドaオキシゲナーゼ(CAO)の蓄積制御機構に異常のあるシロイヌナズナ変異体の解析2008

    • Author(s)
      兼松慧, 櫻庭康仁, 田中亮一, 田中歩
    • Organizer
      第49回日本植物生理学会年会
    • Place of Presentation
      札幌コンベンションセンター、札幌市
    • Year and Date
      2008-03-22
    • Related Report
      2009 Final Research Report 2007 Annual Research Report
  • [Presentation] テトラピロール代謝に関わる新しい因子の探索2008

    • Author(s)
      田中亮一
    • Organizer
      第10回オルガネラワークショップ
    • Place of Presentation
      札幌コンベンションセンター、札幌
    • Year and Date
      2008-03-19
    • Related Report
      2009 Final Research Report
  • [Presentation] テトラピロール代謝に関わる新しい因子の探索2008

    • Author(s)
      田中亮一
    • Organizer
      第10回オルガネラワークショップ
    • Place of Presentation
      札幌コンペンションセンター、札幌
    • Year and Date
      2008-03-19
    • Related Report
      2007 Annual Research Report
  • [Presentation] クロロフィリドaオキシゲナーゼの蓄積量を調節する内部配列の解析2008

    • Author(s)
      櫻庭康仁, 田中亮一, 田中歩
    • Organizer
      第49回日本植物生理学会年会
    • Place of Presentation
      札幌コンベンションセンター、札幌市
    • Related Report
      2009 Final Research Report 2007 Annual Research Report
  • [Presentation] ラン藻Synechocystis sp. PCC 6803由来プロトポルフィリノーゲンIXオキシダーゼ遺伝子の探索2008

    • Author(s)
      加登一成, 田中亮一, 田中歩, 保坂秀夫
    • Organizer
      第49回日本植物生理学会年会
    • Place of Presentation
      札幌コンベンションセンター、札幌市
    • Related Report
      2007 Annual Research Report
  • [Presentation] HPLC-based screening for pigment metabolism mutants in Arabidopsis thaliana.2007

    • Author(s)
      R.Tanaka, T.Nagane, F.Myouga, R.Motohashi, K.Shinozaki, A.Tanaka
    • Organizer
      7^<th> International Conference on Tetrapyrrole Photoreceptor in Photosynthetic Organisms, 7^<th> International Conference on Tetrapyrrole Photoreceptor in Photosynthetic Organisms
    • Place of Presentation
      Kyoto TERRSA, Kyoto
    • Related Report
      2009 Final Research Report
  • [Presentation] (北大低温研、JAMSTEC、産総研、極地研)南極ラングホブデの氷雪藻ブルームにおける微生物群集構造2007

    • Author(s)
      藤井正典、小島久弥、田中亮一、高野淑識、星野保、的場澄人、伊村智、福井学
    • Organizer
      第30回極域生物シンポジウム
    • Place of Presentation
      東京
    • Related Report
      2009 Final Research Report
  • [Presentation] A short sequence in the N-terminal domain of chlorophyllide a oxigenase regulates its accumulation.2007

    • Author(s)
      Yasuhito Sakuraba, Ryouichi Tanaka and Ayumi Tanaka
    • Organizer
      7^<th> International Conference on Tetrapyrrole Photoreceptor in Photosynthetic Organisms
    • Place of Presentation
      Kyoto TERRSA, Kyoto
    • Related Report
      2007 Annual Research Report
  • [Presentation] HPLC-based screening for pigment metabolism mutants in Arabidopsis thaliana., 7^<th> International Conference on Tetrapyrrole Photoreceptor in Photosynthetic Organisms2007

    • Author(s)
      R. Tanaka, T. Nagane, F. Myouga, R. Motohashi, K. Shinozaki and A. Tanaka
    • Organizer
      7^<th> International Conference on Tetrapyrrole Photoreceptor in Photosynthetic Organisms
    • Place of Presentation
      Kyoto TERRSA, Kyoto
    • Related Report
      2007 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.lowtem.hokudai.ac.jp/plantadapt/ayumi/ryouichi/

    • Related Report
      2009 Final Research Report
  • [Remarks]

    • URL

      http://www.lowtem.hokudai.ac.jp/

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.lowtem.hokudai.ac.jp/plantadapt/ayumi/

    • Related Report
      2008 Annual Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi