Crystallographic analyses of functional mechanisms of sensory receptors

Research Project

Project/Area Number	19687006
Research Category	Grant-in-Aid for Young Scientists (A)
Allocation Type	Single-year Grants
Research Field	Structural biochemistry
Research Institution	The Institute of Physical and Chemical Research
Principal Investigator	YAMASHITA Atsuko The Institute of Physical and Chemical Research, 分子シグナリング研究チーム, チームリーダー (10321738)
Project Period (FY)	2007 – 2009
Project Status	Completed (Fiscal Year 2009)
Budget Amount *help	¥25,480,000 (Direct Cost: ¥19,600,000、Indirect Cost: ¥5,880,000) Fiscal Year 2009: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000) Fiscal Year 2008: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000) Fiscal Year 2007: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Keywords	TRPチャネル / 機械刺激受容チャネル / 結晶構造 / タンパク質 / シグナル伝達 / 神経科学 / 構造生物学 / 感覚受容
Research Abstract	We identified a structurally-stable cytosolic domain of a mechanosensitive TRP channel, and found that the domain is a crucial region for its mechanosensitive channel activity. Structural analysis of the domain was performed by X-ray crystallography. A couple of regions in the domain were successfully crystallized and their X-ray diffraction data were collected. Among them, crystallographic structure of one region was determined.