Screening for targets of CDK/cyclin

• Project/Area Number: 19687012
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: Nagoya University
• Principal Investigator: MIMURA Satoru Nagoya University, 大学院・理学研究科, 助教 (60432233)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥25,350,000 (Direct Cost: ¥19,500,000、Indirect Cost: ¥5,850,000); Fiscal Year 2009: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000); Fiscal Year 2008: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000); Fiscal Year 2007: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
• Keywords: DNA複製 / ユビキチンリガーゼ / 細胞周期制御 / 細胞周期 / 出芽酵母 / CDK / リン酸化

Research Abstract

CDK/cyclin regulates the progression of the eukaryotic cell cycle. To understand the function of CDK/cyclin, I investigated the regulation of DNA replication, which is regulated by CDK/cyclin activity. I found that an ubiquitin ligase, SCFDia2 localized at replication fork and regulates its progression, and that Cul8, which is also an ubiquitin ligase, forms varieties of protein complex and participates in the regulation of genome stability.

Research Products

• [Journal Article] Non-SCF-type F-box protein Roy1/Ymr258c interacts with a Rab5-like GTPase Ypt52 and inhibits Ypt 52 function Mol. (2011)
  • Author(s): Liu, Y., Nakatsukasa, K., Kotera, M., Kanada, A., Nishimura, T., Kishi., T., Mimura, S., Kamura, T.
  • Journal Title: Biol. Cell 22 Pages: 1575-1584
  • Peer Reviewed
• [Journal Article] Cul8/Rtt101 forms a variety of protein complexes that regulate DNA damage response and transcriptional silencing. (2010)
  • Author(s): Mimura, S., Yamaguchi, T., Ishii, S., Noro E., Katsura., T., Obuse, C., Kamura, T.
  • Journal Title: J.Biol.Chem. 285 Pages: 9858-9867
  • Peer Reviewed
• [Journal Article] SCFDia2 regulates DNA replication forks during S-phase in buding yeast. (2009)
  • Author(s): Mimura, S., Komata, M., KIshi, T., Shirahige, K., Kamura, T.
  • Journal Title: EMBO J. 28 Pages: 3693-3705
  • Peer Reviewed
• [Journal Article] A longevity protein Lag2, interacts with SCF complex and regulates SCF function. (2009)
  • Author(s): Liu, Y., Mimura, S., Kishi, T., Kamura, T.
  • Journal Title: EMBO J. 28 Pages: 3366-3377
  • Peer Reviewed
• [Presentation] UBIQUITIN LIGASES INVOLVED IN THE REGULATION OF DNA REPLICATION IN BUDDING YEAST (2010)
  • Author(s): Satoru Mimura, Takumi Kamura
  • Organizer: The 7^<th> 3R meeting
  • Place of Presentation: アナクラウンホテル富山
  • Year and Date: 2010-10-29
• [Presentation] 出芽酵母SCFDia2によるDNA 複製フォークの制御 (2009)
  • Author(s): 三村覚、古俣麻希子、岸努、白髭克彦、嘉村巧
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 神奈川県パシフィコ横浜
  • Year and Date: 2009-12-12
• [Presentation] Regulation of DNA replication by E3 Ubiquitin ligase, SCFDia2 (2009)
  • Author(s): Satoru Mimura, Makiko Komata, Tsutomu Kishi, Katsuhiko Shirahige, Takumi Kamura
  • Organizer: International Symposium on Chromosome cycle and genome dynamics
  • Place of Presentation: 栃木県サンバレー那須
  • Year and Date: 2009-11-11
• [Presentation] 安定なDNA 複製に関わる出芽酵母F-box蛋白質Dia2の解析 (2008)
  • Author(s): 三村覚、古俣麻希子、岸努、白髭克彦、嘉村巧
  • Organizer: 第31回日本分子生物学年会第81回日本生化学会大会合同大会,BMB2008
  • Place of Presentation: 兵庫県神戸国際会議場
  • Year and Date: 2008-12-11
• [Presentation] 安定なDNA複製に関わる出芽酵母F-box蛋白質Dia2の解析 (2008)
  • Author(s): 三村覚、古俣麻希子、岸努、白髭克彦、嘉村巧
  • Organizer: BMB2008(第31回日本分子生物学会年会第81回日本生化学会大会合同大会)
  • Place of Presentation: 兵庫県神戸神戸ポートアイランド
  • Year and Date: 2008-12-11
• [Presentation] Regulation of DNA replication by E3 Ubiquitin ligase, SCFDia2 (2008)
  • Author(s): Satoru Mimura, Makiko Komata, Tsutomu kishi, Katsuhiko Shirahige, Takumi Kamura
  • Organizer: International Symposium on Chromosome Dynamics in Ise
  • Place of Presentation: 静岡県掛川つま恋リゾート
  • Year and Date: 2008-10-28
• [Presentation] Regulation of DNA Replication by E 3 ubiquitin ligase, SCE^<Dia 2> (2008)
  • Author(s): Satoru Mimura, Makiko Komata, Tsutomu Kishi, Katsuhiko Shirahige, Takumi Kamura
  • Organizer: The 6^<th> Symposium
  • Place of Presentation: 静岡県掛川つま恋リゾート
  • Year and Date: 2008-10-28
• [Presentation] Regulation of DNA replication by E3 Ubiquitin ligase, SCFDia2 (2008)
  • Author(s): Satoru Mimura, Tsutomu kishi, Takumi Kamura
  • Organizer: International Symposium on Chromosome Dynamics in Ise
  • Place of Presentation: 三重県伊勢志摩ホテル近鉄アクアビラ
  • Year and Date: 2008-05-29
• [Presentation] Regulation of DNA Replication by E 3 ubiquitin ligase, SCF^<Dia 2> (2008)
  • Author(s): Satoru Mimura, Tsutomu Kishi, Takumi Kamura
  • Organizer: International Symposium on Chromosome Dynamics in Ise
  • Place of Presentation: 三重県伊勢志摩ホテル近鉄アクアビラ
  • Year and Date: 2008-05-29
• [Presentation] 出芽酵母F-Box蛋白質Dia2と複製フォーク蛋白質との結合 (2007)
  • Author(s): 三村覚、嘉村巧
  • Organizer: 第30回日本分子生物学年会第81回日本生化学会大会合同大会,BMB2007
  • Place of Presentation: 神奈川県パシフィコ横浜
  • Year and Date: 2007-12-11