Clarification both of function and regulation of protein kinase NLK, that fine-tunes the activity of multiple intracellular signaling pathway.

• Project/Area Number: 19687013
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: Kyushu University
• Principal Investigator: ISHITANI Tohru Kyushu University, 生体防御医学研究所, 特任准教授 (40448428)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥25,350,000 (Direct Cost: ¥19,500,000、Indirect Cost: ¥5,850,000); Fiscal Year 2009: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000); Fiscal Year 2008: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000); Fiscal Year 2007: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
• Keywords: Nemo-like kinase / ゼブラフィッシュ / Wntシグナル / Notchシグナル / NLK / NGFシグナル / Notch シグナル

Research Abstract

Nemo-like kinase is a conserved protein kinase. In this study, we found that NLK promotes neurogenesis via phosphorylating Notch1 and that NLK contribute to neurite outgrowth via phosphorylating paxillin and MAP1B downstream of NGF signaling.

Research Products

• [Journal Article] an essential effector of anterior formation, functions downstream of p38 MAP kinase. (2010)
  • Author(s): Ohnishi E, Goto T, Sato A, Kim MS, Iemura SI, Ishitani T, Natsume T, Ohnishi J, Shibuya H. NLK
  • Journal Title: Molecular and Cellular Biology 30巻 Pages: 675-683
  • Peer Reviewed
• [Journal Article] Nemo-like kinase suppresses Notch signalling by interfering with formation of the Notch active transcrioptional complex. (2010)
  • Author(s): Ishitani T, Hirao T, Suzuki M, Isoda M, Ishitani S, Harigaya K, Kitagawa M, Matsumoto K, Itoh M.
  • Journal Title: Nature Cell Biology 12巻 Pages: 278-285
  • Peer Reviewed
• [Journal Article] KDM7 is a dual demethylase for histone H3 lysines 9 and 27 and functions in brain development. (2010)
  • Author(s): Tsukada Y, Ishitani T, Nakayama KI.
  • Journal Title: Genes & Development 24巻 Pages: 432-437
  • Peer Reviewed
• [Journal Article] NLK, an essential effector of anterior formation, functions downstream of p38 MAP kinase. (2010)
  • Author(s): Ohnishi E, Goto T, Sato A, Kim MS, Iemura SI, Ishitani T, Natsume T, Ohnishi J, Shibuya H.
  • Journal Title: Molecular and Cellular Biology. 30 Pages: 675-683
  • Peer Reviewed
• [Journal Article] Nemo like kinase suppresses Notch signalling by interfering with formation of the Notch active transcrioptional complex. (2010)
  • Author(s): Ishitani T, Hirao T, Suzuki M, Isoda M, Ishitani S, Harigaya K, Kitagawa M, Matsumoto, K, Itoh M
  • Journal Title: Nature Cell Biology. 12 Pages: 278-285
  • Peer Reviewed
• [Journal Article] KDM7 is a dual demethylase for histone H3 lysines 9 and 27 and functions in brain development. (2010)
  • Author(s): Tsukada Y, Ishitani T, Nakayama KI.
  • Journal Title: Genes & Development. 24
  • Peer Reviewed
• [Journal Article] Nemo-like kinase is involved in NGF-induced neurite outgrowth via phosphorylating MAP1B and paxillin. (2009)
  • Author(s): Ishitani T, Ishitani S, Matsumoto K, Itoh M.
  • Journal Title: Journal of Neurochemistry 111巻 Pages: 1104-1118
  • Peer Reviewed
• [Journal Article] Homeodomain-interacting protein kinases (Hipks) promote Wnt/Wg signaling through stabilization of β-catenin/Arm and stimulation of target gene expression. (2009)
  • Author(s): Lee W, Swarup S, Chen J, Ishitani T, Verheyen EM
  • Journal Title: Development 136巻 Pages: 241-251
  • Peer Reviewed
• [Journal Article] Nemo-like kinase is involved in NGF-induced neurite outgrowth via phosphorylating MAP1B and paxillin. (2009)
  • Author(s): Ishitani T, Ishitani S, Matsumoto K, Itoh M.
  • Journal Title: Journal of Neurochemistry 111 Pages: 1104-1118
  • Peer Reviewed
• [Presentation] Nemo-like kinase suppresses Notch signalling by interfering with formation of the Notch active transcriptional complex. (2010)
  • Author(s): Tohru Ishitani, Tomoko Hirao, Maho Suzuki, Miho Isoda, Shizuka Ishitani, Kenichi Harigaya, Motoo Kitagawa, Kunihiro Matsumoto, Motoyuki Itoh
  • Organizer: Keystone Symposia, Integration of Developmental Signaling Pathways
  • Place of Presentation: Victoria, Canada
  • Year and Date: 2010-03-24
• [Presentation] Nemo-like kinase suppresses Notch signalling by interfering with formation of the Notch active transcriptional complex. (2010)
  • Author(s): Tohru Ishitani, Tomoko Hirao, Maho Suzuki, Miho Isoda, Shizuka Ishitani, Kenichi Harigaya, Motoo Kitagawa, Kunihiro Matsumoto, Motoyuki Itoh
  • Organizer: Keystone Symposia, Integration of Developmental Signaling Pathways
  • Place of Presentation: Fairmont Empress Hotel, Victoria, Canada
  • Year and Date: 2010-03-24
• [Presentation] Nemo-like kinase suppresses Notch signalling by interfering with formation of the Notch active transcriptional complex. (2010)
  • Author(s): Tohru Ishitani, Tomoko Hirao, Maho Suzuki, Miho Isoda, Shizuka Ishitani, Kenichi Harigaya, Motoo Kitagawa, Kunihiro Matsumoto, Motoyuki Itoh
  • Organizer: 5th Global-COE International Symposium Cell Cycle and Differentiation
  • Place of Presentation: Temasek Life Sciences Laboratory, Singapore
  • Year and Date: 2010-02-24
• [Presentation] Nemo-like kinase suppresses Notch signalling by interfering with formation of the Notch active transcriptional complex. (2010)
  • Author(s): Tohru Ishitani, Tomoko Hirao, Maho Suzuki, Miho Isoda, Shizuka Ishitani, Kenichi Harigaya, Motoo Kitagawa, Kunihiro Matsumoto, Motoyuki Itoh
  • Organizer: 5^<th> Global-COE International Symposium Cell Cycle and Differentiation
  • Place of Presentation: Temasek Life Sciences Laboratory, Singapore
  • Year and Date: 2010-02-24
• [Presentation] Nemo-like kinase in a context-dependent regulator of Wnt signaling that either promotes or inhibits Lefl-mediated transactivation. (2009)
  • Author(s): 太田聡、石谷閑、清水誠之、松本邦弘、伊藤素行、石谷太
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: バシフィコ横浜
  • Year and Date: 2009-12-19
• [Presentation] Homeodomain interacting protein kinase 2 promotes Wnt signaling via Lefl transcription factor. (2009)
  • Author(s): 清水誠之、東谷光洋、石谷太
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: バシフィコ横浜
  • Year and Date: 2009-12-19
• [Presentation] からだの形作りを担うシグナルネットワーク (2009)
  • Author(s): 石谷太
  • Organizer: 第五回北海道大学・九州大学合同報告会
  • Place of Presentation: 都市センターホテル
  • Year and Date: 2009-12-10
• [Presentation] Homeodomain interacting protein kinase 2 promotes Wnt signaling via Lef1 transcription factor. (2009)
  • Author(s): 清水誠之、東谷光洋、石谷太
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-09
• [Presentation] Nemo-like kinase is a context-dependent regulator of Wnt signaling that either promotes or inhibits Lef1-mediated transactivation. (2009)
  • Author(s): 太田聡、石谷閑、清水誠之、松本邦弘、伊藤素行、石谷太
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-09
• [Presentation] Nemo-like kinaseによるシグナル制御～分子/細胞/組織レベルにおけるシグナル伝逹の統合的理解を目指して～ (2009)
  • Author(s): 石谷太
  • Organizer: 日本発生生物学会秋季シンポジウム
  • Place of Presentation: 東レ総合研修センター
  • Year and Date: 2009-11-27
• [Presentation] Nemo-like kinaseはNotch転写複合体の形成を阻害することによりNotchシグナルを抑制する (2009)
  • Author(s): Tohru Ishitani, Tomoko Hirao, Maho Suzuki, Miho Isoda, Shizuka Ishitani, Kenichi Harigaya, Motoo Kitagawa, Kunihiro Matsumoto, Motoyuki Itoh
  • Organizer: 第82回日本生化学会大会、口頭発表
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2009-10-24
• [Presentation] Nemo-like kinase はNotch転写複合体の形成を阻害することによりNotchシグナルを抑制する (2009)
  • Author(s): Tohru Ishitani, Tomoko Hirao, Maho Suzuki, Miho Isoda, Shizuka Ishitani, Kenichi Harigaya, Motoo Kitagawa, Kunihiro Matsumoto, Motoyuki Itoh
  • Organizer: 第82回日本生化学会大学
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2009-10-24
• [Presentation] ゼブラフィッシュにおけるシグナル伝達可視化の試み (2009)
  • Author(s): 太田聡、清水誠之、石谷太
  • Organizer: 第15回小型魚類研究会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-09-12
• [Presentation] Nemo-like kinaseはLef1のリン酸化を介してWntシグナルを正に制御し、中脳視蓋の形成に貢献する (2009)
  • Author(s): 太田聡、石谷閑、清水誠之、松本邦弘、伊藤素行、石谷太
  • Organizer: 第15回小型魚類研究会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-09-12
• [Presentation] Roles of Hipk2 in zebrtafish development (2009)
  • Author(s): Nobuyuki Shimizu, Mitsuhiro Tohtani, Tohru Ishitani
  • Organizer: 第42回日本発生生物学会大会
  • Place of Presentation: 朱鷺メッセ
  • Year and Date: 2009-05-29
• [Presentation] Hipks promote Wnt/Wg signaling through stabilization of beta-catenin/Arm. (2009)
  • Author(s): Wendy Lee, Tohru Ishitani, Esther Verheyen
  • Organizer: 第42回日本発生生物学会大会
  • Place of Presentation: 朱鷺メッセ
  • Year and Date: 2009-05-29
• [Presentation] Roles of Hipk2 in zebrafish development (2009)
  • Author(s): Nobuyuki Shimizu, Mitsuhiro Tohtani, Tohru Ishitani
  • Organizer: 第42回日本発生生物学会大会
  • Place of Presentation: 新潟 朱鷺メッセ
  • Year and Date: 2009-05-29
• [Presentation] Hipks promote Wnt/Wg signaling through stabilization of beta-catenin/Arm. (2009)
  • Author(s): Wendy Lee, Tohru Ishitani, Esther Verheyen
  • Organizer: 第42回日本発生生物学会大会
  • Place of Presentation: 新潟 朱鷺メッセ
  • Year and Date: 2009-05-29
• [Presentation] Nemo-like kinase is a critical mediator of NGF-regulated axonal development. (2009)
  • Author(s): Tohru Ishitani, Shizuka Ishitani, Kunihiro Matsumoto
  • Organizer: 第41回日本発生生物学会大会
  • Place of Presentation: 徳島
  • Year and Date: 2009-05-28
• [Presentation] 転写因子Tcf/Lefの修飾による制御と生理学的意義の解析 (2009)
  • Author(s): 石谷太
  • Organizer: 九州大学グローバルCOE/SSP共催シンポジウム「Wntシグナルの機能と制御:基礎研究から再生医療、がん治療へ」
  • Place of Presentation: 九州大学
  • Year and Date: 2009-03-24
• [Presentation] Nemo-like kinase promotes neurogenesis by interfering with the Notch signaling pathway. (2009)
  • Author(s): Tohru Ishitani
  • Organizer: Global-COE Singapore Symposium
  • Place of Presentation: Rendezvous Hotel, Singapore
  • Year and Date: 2009-02-16
• [Presentation] Nemo-like kinase suppresses Notch signalling by interfering with formation of the Notch active transcriptional complex. (2009)
  • Author(s): Tohru Ishitani
  • Organizer: The Joint Symposium of the 4th International Symposium of Institutes Network and Osaka University Global COE (Frontier Biomedical Science Underlying Organelle Network Biology) Symposium
  • Place of Presentation: ホテル阪急エキスポパーク
  • Year and Date: 2009-01-31
• [Presentation] Nemo-like kinase suppresses Notch signalling by interfering with forma tion of the Notch active transcriptional complex (2009)
  • Author(s): Tohru Ishitani
  • Organizer: The Joint Symposium of the 4th International Symposium of Institutes Network and Osaka University Global COE (Frontier Biomedical Science Underlying Organelle Network Biology) Symposium
  • Place of Presentation: ホテル阪急エキスポパーク
  • Year and Date: 2009-01-31
• [Presentation] Nemo-like kinaseはNotchシグナルを阻害し、神経分化を制御する (2008)
  • Author(s): 石谷太、平尾智子、鈴木真帆、磯田美 帆、北川元生、松本邦弘、伊藤素行
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] Nemo-likekinaseはNotchシグナルを阻害し、神経分化を制御する (2008)
  • Author(s): 石谷太、平尾智子、鈴木真帆、磯田美帆、北川元生、松本邦弘、伊藤素行
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会ワークショップ
  • Place of Presentation: 神戸ポートピアホテル
  • Year and Date: 2008-12-11
• [Presentation] Nemo-like kinaseはNotchシグナルを阻害し、神経分化を制御する (2008)
  • Author(s): 石谷太、平尾智子、鈴木真帆、磯田 美帆、北川元生、松本邦弘、伊藤素行
  • Organizer: 第3回Notch研究会
  • Place of Presentation: 国立遺伝学研究所
  • Year and Date: 2008-07-18
• [Presentation] Nemo-like kinase is a critical mediator of NGF-regulated axonal develo pment. Tohru Ishitani, Shizuka Ishitani, Kunihiro Matsumoto (2008)
  • Author(s): Tohru Ishitani, Shizuka Ishitani, Kunihiro Matsumoto
  • Organizer: 第41回日本発生生物学会大会ワークショップ
  • Place of Presentation: 徳島県郷土文化会館
  • Year and Date: 2008-05-28
• [Presentation] Nemo-like kinaseはNotchシグナルを阻害し、神経分化を制御する (2008)
  • Author(s): 石谷太、平尾智子、鈴木真帆、磯田美 帆、松本邦弘、伊藤素行
  • Organizer: 第41回日本発生生物学会大会
  • Year and Date: 2008-05-27
• [Presentation] Nemo-iikekinaseはNotchシグナルを阻害し、神経分化を制御する (2008)
  • Author(s): 石谷太、平尾智子、鈴木真帆、磯田美帆、松本邦弘、伊藤素行
  • Organizer: 第41回日本発生生物学会大会若手ワークショップ
  • Place of Presentation: 徳島県郷土文化会館
  • Year and Date: 2008-05-27
• [Presentation] NGFの制御する神経軸索形成におけるNemo-1ike kinaseの機能 (2007)
  • Author(s): 石谷 太、松本邦弘、伊藤素行
  • Organizer: 分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-11
• [Presentation] Nemo-like kinase はNotch シグナル伝達系を負に制御することにより神経発生を促進する (2007)
  • Author(s): 石谷 太、松本邦弘、伊藤素行
  • Organizer: 第40回日本発生生物学会・第59回日本細胞生物学会合同大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2007-05-29
• [Presentation] NGFの制御する神経軸索形成におけるNemo-like kinaseの機能 (2007)
  • Author(s): 石谷太、石谷閑、松本邦弘、伊藤素行
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Nemo-like kinase promotes neurogenesis by blocking Notch-signaling (2007)
  • Author(s): Tohru Ishitani, Tomoko Hirao, Maho Suzuki, Miho Isoda, Kunihiro Matsumoto, Motoyuki Itoh
  • Organizer: 第40回日本発生生物学会・第59回日本細胞生物学会合同大会
  • Place of Presentation: 福岡国際会議場
• [Book] 実験医学hot topics(Nemo-like kinaseはNotch転写複合体の形成を阻害し、神経細胞形成を促進する) (2010)
  • Author(s): 石谷太、松本邦弘、伊藤素行
  • Publisher: 羊土社(in press)
• [Book] 医学のあゆみ(転写因子TCF/LEFの修飾によるWntシグナル制御) (2010)
  • Author(s): 石谷太、石谷閑
  • Publisher: 医歯薬出版(in press)
• [Book] 実験医学「hot topics"Nemo-like kinaseはNotch転写複合体の形成を阻害し、神経細胞形成を促進する"」 (2010)
  • Author(s): 石谷太、松本邦弘、伊藤素行
  • Total Pages: 4
  • Publisher: 羊士社(in press)(予定)
• [Book] 医学のあゆみ「Wntシグナル・ネットワーク研究"転写因子TCF/LEFの修飾によるWntシグナル制御"」 (2010)
  • Author(s): 石谷太、石谷閑
  • Publisher: 医歯薬出版(in press)(予定)
• [Remarks]
  • URL: http://www.bioreg.kyushu-u.ac.jp/labo/crs/top.html
• [Remarks]
  • URL: http://www.bioreg.kyushu-u.ac.jp/labo/crs/top.html
• [Remarks]
  • URL: http://www.bioreg.kyushu-u.ac.jp/labo/crs/top.html