Elucidating the molecular mechanisms of neuronal polarity with genetic mosaic methods

• Project/Area Number: 19687014
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: The University of Tokyo
• Principal Investigator: CHIHARA Takahiro The University of Tokyo, 大学院・薬学系研究科, 講師 (00431891)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥25,350,000 (Direct Cost: ¥19,500,000、Indirect Cost: ¥5,850,000); Fiscal Year 2009: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000); Fiscal Year 2008: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000); Fiscal Year 2007: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
• Keywords: 形態形成 / 神経極性 / 神経発生生物学 / モザイク解析 / ショウジョウバエ / 樹状突起 / 軸索

Research Abstract

To form functional neural circuits like brain, each neuron must possess extremely polarized cellular structures, dendrites and an axon. In this study, we utilized various Drosophila molecular genetic methods to elucidate the molecular mechanisms for neuronal polarization in vivo. Molecular genetic analyses of several mutants defective in neuronal polarization and circuit formation revealed the importance of sugar modification on cell surface proteins.

Research Products

• [Journal Article] Histon deacetylase Rpd3 regulates olfactory projection neuron dendrite targeting via the transcription factor Prospero. (2010)
  • Author(s): Joy S.Tea, Takahiro Chihara, Liqun Luo.
  • Journal Title: The Journal of Neuroscience. 30 Pages: 9939-9946
  • Peer Reviewed
• [Journal Article] Lineage-specific effects of Notch/Numb signaling in post-embryonic development of the Drosophila brain. (2010)
  • Author(s): Suewei Lin, Sen-Lin Lai, Huang-Hsiang Yu, Takahiro Chihara, Liqun Luo, Tzumin Lee.
  • Journal Title: Development. 137 Pages: 43-51
  • Peer Reviewed
• [Journal Article] Lineage-specific effects of Notch/Numb signaling in post-embryonic development of the Drosophila brain (2010)
  • Author(s): Suewei Lin, Sen-Lin Lai, Huang-Hsiang Yu, Takahiro Chihara, Liqun Luo, Tzumin Lee
  • Journal Title: Development Volume: 137 Pages: 43-51
  • Peer Reviewed
• [Journal Article] Histon deacetylase Rpd3 regulates olfactory projection neuron dendrite targeting via the transcription factor Prospero (2010)
  • Author(s): Joy S.Tea, Takahiro Chihara, Liqun Luo
  • Journal Title: The Journal of Neuroscience Volume: 30 Pages: 9939-46
  • Peer Reviewed
• [Journal Article] Organelles in developing neurons : Essential regulators of neuronal morphogenesis and function. (2009)
  • Author(s): Sayaka Sekine, Masayuki Miura, Takahiro Chihara.
  • Journal Title: The International Journal of Developmental Biology. 53 Pages: 19-27
  • Peer Reviewed
• [Journal Article] Organelles in developing neurons : Essential regulators of neuronal morphogenesis and function (2009)
  • Author(s): Sayaka Sekine, Masayuki Miu ra and Takahiro Chihara
  • Journal Title: The International Journal of Developmental Biology 53 Pages: 19-27
  • Peer Reviewed
• [Journal Article] Cytoplasmic and mitochondrial protein translation in axonal and dendritic terminal arborization. (2007)
  • Author(s): Takahiro Chihara, David Luginbuhl, Liqun Luo.
  • Journal Title: Nature Neuroscience. 10 Pages: 828-837
  • Peer Reviewed
• [Journal Article] Cytoplasmic and mitochondrial protein translation in axonal and dendritic terminal arborization (2007)
  • Author(s): Takahiro Chihara, David Luginbuhl and Liqun Luo
  • Journal Title: Nature Neuroscience 10 Pages: 828-837
  • Peer Reviewed
• [Presentation] メンター:研究人生における最強の味方 (2010)
  • Author(s): Takahiro Chihara
  • Organizer: The 43^<rd> Annual meeting for the Japanese society of developmental biologists
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2010-06-21
• [Presentation] Neuronal wiring in the olfactory system requires a novel targeting molecule, Dogi. (2010)
  • Author(s): Chisako Ando, Liqun Luo, Masayuki Miura, Takahiro Chihara.
  • Organizer: The 13^<th> European Drosophila neurobiology conference.
  • Place of Presentation: Manchester, UK
• [Presentation] Dendritic and axonal targeting along medial to lateral axis in the antennal lobe are regulated by Meigo, a putative UDP-sugar transporter. (2010)
  • Author(s): Sayaka Sekine, Liqun Luo, Masayuki Miura, Takahiro Chihara.
  • Organizer: The 51^<st> Annual Drosophila Research Conference
  • Place of Presentation: Washington DC, USA
• [Presentation] Dendritic and axonal targeting along medial to lateral axis in the antennal lobe are regulated by Meigo, a putative UDP-sugar transporter (2010)
  • Author(s): Sayaka Sekine, Liqun Luo, Masayuki Miura, Takahiro Chihara
  • Organizer: The 51^<st> Annual Drosophila Research Conference
  • Place of Presentation: Washington DC, USA
• [Presentation] Neuronal wiring in the olfactory system requires a novel targeting molecule, Dogi (2010)
  • Author(s): Chisako Ando, Liqun Luo, Masayuki Miura, Takahiro Chihara
  • Organizer: The 13^<th> European Drosophila neurobiology conference
  • Place of Presentation: Manchester, UK
• [Presentation] 脳内単一脳細胞モザイク解析法を用いて神経回路形成を理解する (2009)
  • Author(s): Takahiro Chihara.
  • Organizer: Symposium on "Biological Sciences" at Kumamoto University.
  • Place of Presentation: Kumamoto, Japan
  • Year and Date: 2009-10-16
• [Presentation] 脳内単一脳細胞モザイク解析法を用いて神経回路形成を理解する (2009)
  • Author(s): Takahiro Chihara
  • Organizer: Symposium on "Biological Sciences" at Kumamoto University
  • Place of Presentation: Kumamoto, Japan
  • Year and Date: 2009-10-16
• [Presentation] Meigo, a putative sugar transporter, regulates dendritic but not axonal targeting in Drosophila olfactory projection neurons (2009)
  • Author(s): Sayaka Sekine, Liqun Luo, Masayuki Miura, Takahiro Chihara
  • Organizer: The 42^<nd> Annual meeting for the Japanese society of Developmental biologists
  • Place of Presentation: Niigata, Japan
  • Year and Date: 2009-05-30
• [Presentation] ショウジョウバエ脳内の樹状突起ターゲティングを支える分子基盤国立遺伝学研究所研究会 (2009)
  • Author(s): 千原崇裕
  • Organizer: モデル生物における神経系の遺伝学 -発生から行動まで-
  • Place of Presentation: 三島・静岡県
  • Year and Date: 2009-03-27
• [Presentation] ショウジョウバエ脳内の樹状突起ターゲティングを支える分子基盤 (2009)
  • Author(s): 千原崇裕
  • Organizer: 国立遺伝学研究所研究会「モデル生物における神経系の遺伝学-発生から行動まで-」
  • Place of Presentation: 三島、静岡県
  • Year and Date: 2009-03-27
• [Presentation] Evolutionarily conserved protein, Dogi regulates dendritic targeting and axonal guidance of Drosophila olfactory projection neurons. (2009)
  • Author(s): Chisako Ando, Liqun Luo, Masayuki Miura, Takahiro Chihara.
  • Organizer: 50^<th> Annual Drosophila Research Conference.
  • Place of Presentation: Chicago, USA
• [Presentation] Dogi : an evolutionarily conserved protein is cell-autonomously required for dendritic targeting and axonal guidance of Drosophila olfactory projection neurons (2009)
  • Author(s): Chisako Ando, Liqun Luo, Masayuki Miura, Takahiro Chihara
  • Organizer: The 42^<nd> Annual meeting for the Japanese society of Developmental biologists
  • Place of Presentation: Niigata, Japan
• [Presentation] Meigo, a putative UDP-sugar transporter, regulaters dendritic but not axonal targeting of the olfactgory projection neuron (2009)
  • Author(s): Sayaka Sekine, Liqun Luo, Masayuki Miura, Takahiro Chihara
  • Organizer: The 9^<th> Japanese Drosophila research conference
  • Place of Presentation: Kakegawa, Japan
• [Presentation] Evolutionarily conserved protein, Dogi regulates dendritic targeting and axonal guidance of Drosophila olfactory projection neurons (2009)
  • Author(s): Chisako Ando, Liqun Luo, Masayuki Miura, Takahiro Chihara
  • Organizer: The 9^<th> Japanese Drosophila research conference
  • Place of Presentation: Kakegawa, Japan
• [Presentation] Identifying neurons with caspase activity in Drosophila adult brain (2009)
  • Author(s): Aki Kitabayashi, Takahiro Chihara, Masayuki Miura
  • Organizer: The 9^<th> Japanese Drosophila research conference
  • Place of Presentation: Kakegawa, Japan
• [Presentation] Caspase activation in developing neurons : evaluation of PARP-Venus, a genetically encoded probe for caspase activation (2009)
  • Author(s): Michie Morimoto, Takahiro Chihara, Masayuki Miura
  • Organizer: The 9^<th> Japanese Drosophila research conference
  • Place of Presentation: Kakegawa, Japan
• [Presentation] Evolutionarily conserved protein, Dogi regulates dendritic targeting and axonal guidance of Drosophila olfactory projection neurons (2009)
  • Author(s): Chisako Ando, Liqun Luo, Masayuki Miura and Takahiro Chihara
  • Organizer: 50^<th> Annual Drosophila Research Conference
  • Place of Presentation: Chicago, USA
• [Presentation] 嗅覚系二次神経の軸索・樹状突起ガイダンスを制御する分子機構 金沢大学革新脳科学COEシンポジウム (2008)
  • Author(s): 千原崇裕
  • Organizer: Neurobiology of Drosophila
  • Place of Presentation: 金沢・石川県
  • Year and Date: 2008-08-21
• [Presentation] 嗅覚系二次神経の軸索・樹状突起ガイダンスを制御する分子機構 (2008)
  • Author(s): 千原崇裕層
  • Organizer: 金沢大学革新脳科学COEシンポジウム Neurobiology of Drosophila
  • Place of Presentation: 金沢、石川県
  • Year and Date: 2008-08-21
• [Presentation] Histone deacetylase 1 (Rpd3) in dendritic targeting of Drosophila olfactory projection neurons. (2008)
  • Author(s): Takahiro Chihara, Joy S.Wu, Liqun Luo.
  • Organizer: 49^<th> Annual Drosophila Research Conference.
  • Place of Presentation: San Diego, USA
• [Presentation] Histone deacetylase 1 (Rpd3)in dendritic targeting of Drosophila olfactory projection neurons (2008)
  • Author(s): Takahiro Chihara, Joy S. Wu and Liqun Luo
  • Organizer: 49^<th> Annual Drosophila Research Conference
  • Place of Presentation: San Diego, USA
• [Presentation] Secretory pathway contributes to dendrite-specific targeting in Drosophila olfactory projection neurons (2008)
  • Author(s): Sayaka Sekine, Liqun Luo, Masayuki Miura and Takahiro Chihara
  • Organizer: Neuroscience 2008
  • Place of Presentation: Tokyo, Japan
• [Presentation] Genetic mosaic analysis of dendritic guidance in Drosophilaolfactory projection neurons (2008)
  • Author(s): Takahiro Chihara, Chisako. Ando, Liqun Luo and Masayuki Miura
  • Organizer: Neuroscience 2008
  • Place of Presentation: Tokyo, Japan
• [Presentation] Identifying neurons with caspase activation in Drosophila central nervous system (2008)
  • Author(s): Aki Kitabayashi, Michie Morimoto, Takahiro Chihara and Masayuki Miura
  • Organizer: The 15^<th> Takeda Science Foundation Symposium on Bioscience : Cell Death
  • Place of Presentation: Tokyo, Japan
• [Presentation] ショウジョウバエ嗅覚系投射神経における樹状突起ガイダンスの遺伝学的モザイク解析 (2008)
  • Author(s): 安東知佐子、千原崇裕、Liqu nLuo、三浦正幸
  • Organizer: BMB2008(第31回・日本分子生物学会年会・第81回日本生化学会大会・合同大会)
  • Place of Presentation: 神戸、兵庫県
• [Presentation] Genetic mosaic analysis in Drosophila brain reveals specialized functions of house-keeping genes in neuronal morphogenesis. (2007)
  • Author(s): Takahiro Chihara, Joy S.Wu, David Luginbuhl, Masayuki Miura, Liqun Luo.
  • Organizer: BMB2007(第30回日本分子生物学会年会・第80回日本生化学会大会・合同大会)
  • Place of Presentation: パシフィコ横浜・神奈川県
• [Presentation] Genetic analysis of dendritic morphogenesis : Neuronal polarity, targeting and maintenance. (2007)
  • Author(s): Takahiro Chihara, David Luginbuhl, Liqun Luo.
  • Organizer: The 8^<th> Japanese Drosophila Research Conference.
  • Place of Presentation: Awaji Yumebutai International Conference Center, Hyogo, Japan
• [Presentation] Genetic analysis of dendritic morphogenesis: Neuronal polarity, targeting and maintenance (2007)
  • Author(s): Takahiro Chihara, David Luginbuhl and Liqun Luo
  • Organizer: The 8th Japanese Drosophila Research Conference
  • Place of Presentation: Awaji Yumebutai International Conference Center, Hyogo, Japan
• [Presentation] Phenotypic analysis of a mutant defective in dendritic but not axonal targeting of Drosophila olfactory projection neuron (2007)
  • Author(s): Sayaka Sekine, Liqun Luo, Masayuki Miura and Takahiro Chihara
  • Organizer: The 8th Japanese Drosophila Research Conference
  • Place of Presentation: Awaji Yumebutai International Conference Center, Hyogo, Japan
• [Presentation] Genetic mosaic analysis in Drosophila brain reveals specialized functions of house-keeping genes in neuronal morphogenesis (2007)
  • Author(s): Takahiro Chihara, Joy S. Wu, David Luginbuhl, Masayuki Miura and Liqun Luo
  • Organizer: BMB2007(第30回日本分子生物学会年会・第80回日本生化学会大会・合同大会)
  • Place of Presentation: パシフィコ横浜、神奈川県
• [Remarks] ホームページ等
  • URL: http://www.f.u-tokyo.ac.jp/~genetics/index.html