Genetic analysis of hybrid breakdown in rice

• Project/Area Number: 19688002
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Breeding science
• Research Institution: Nagoya University
• Principal Investigator: ASHIKARI Motoyuki Nagoya University, 生物機能開発利用研究センター, 教授 (80324383)
• Project Period (FY): 2007 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥20,410,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥4,710,000); Fiscal Year 2010: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2008: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2007: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
• Keywords: イネ / 生殖的隔離 / 雑種崩壊

Research Abstract

Reproductive barriers are important for the maintenance of species identity. We discovered a reproductive barrier via hybrid breakdown among the progeny of a cross between the japonica rice cultivar Koshihikari and the indica rice cultivar Habataki. Genetic analysis indicated that the hybrid breakdown is regulated by the interaction of two recessive genes: hbd2 in Habataki and hbd3 in Koshihikari. Linkage mapping showed that hbd2 is located near the 100 cM region of chromosome 2 in Habataki, whereas hbd3 is located near the 60 cM region of chromosome 11 in Koshihikari. Fine mapping of hbd2 resulted in the identification of the causal gene as casein kinase I (CKI1). Further analysis revealed that hbd2-CKI1 allele gains its deleterious function that causes the weakness phenotype by a change of one amino acid. As for the other gene, hbd3 was mapped to the NBS-LRR gene cluster region. It is the most common class of R-gene that triggers the immune signal in response to pathogen attack. Expression analysis of pathogen response marker genes suggested that weakness phenotype in this hybrid breakdown can be attributed to an autoimmune response. This finding provides a new insight in understanding the molecular and evolutionary mechanisms establishing reproductive barriers in plants.

Research Products

• [Journal Article] Gain of deleterious function causes an autoimmune response and Bateson-Dobzhansky-Muller incompatibility in rice. (2010)
  • Author(s): Yamamoto E, Takashi T, Morinaka Y, Lin SY, Wu JZ, Matsumoto T, Kitano H, Matsuoka M, Ashikari M.
  • Journal Title: Molecular Genetics and Genomics. 283 Pages: 305-315
• [Journal Article] Gain of deleterious function causes an autoimmune response and Bateson-Dobzhansky-Muller incompatibility in rice. (2010)
  • Author(s): Yamamoto E, Takashi T, Morinaka Y, Lin S, Wu J, Matsumoto T, Kitano H, Matsuoka M, Ashikari M.
  • Journal Title: Mol. Genet. Genomics. 283(4) Pages: 305-315
  • Peer Reviewed
• [Journal Article] Gain of deleterious function causes an autoimmune response and Bateson-Dobzhansky-Muller incompatibility in rice (2010)
  • Author(s): Yamqamoto E, Takashi T, Morinaka Y, Lin SY, Wu JZ, Matsumoto T, Kitano H, Matsuoka M, Ashikari M
  • Journal Title: Molecular Genetics and Genomics 283 Pages: 305-315
  • Peer Reviewed
• [Journal Article] Interaction of two recessive genes, hbd2 and hbd3, induces hybrid breakdown in rice. (2007)
  • Author(s): Yamamoto, E., Takashi, T., Morinaka, Y., Lin, S., Kitano, H., Matsuoka, M., Ashikari, M.
  • Journal Title: Theoretical and applied genetics. 115 Pages: 187-194
• [Journal Article] Interaction of two recessive genes. hbd2 and hbd3, induces hybrid breakdown in rice. (2007)
  • Author(s): Yamamoto, E., Takashi, T., Morinaka, Y., Lin, S., Kitano, H., Matsuoka, M. and Ashikari, M.
  • Journal Title: Theoretical and applied genetics 115 Pages: 187-194
  • Peer Reviewed
• [Presentation] イネにおける自己免疫応答と生殖的隔離 (2010)
  • Author(s): 山本英司, 高師知紀, 森中洋一, 林少揚, 呉健忠, 松本隆, 北野英己, 松岡信, 芦苅基行
  • Organizer: イネ遺伝学・分子生物学ワークショップ2010
  • Place of Presentation: 名古屋大学野依記念学術交流館
  • Year and Date: 2010-07-12
• [Presentation] イネにおける接合後隔離機構 (2010)
  • Author(s): 山本英司
  • Organizer: 特定領域研究「植物ゲノム障壁」若手ワークショップ
  • Place of Presentation: 名古屋大学野依記念学術交流館
  • Year and Date: 2010-07-12
• [Presentation] イネにおける自己免疫応答と生殖的隔離 (2010)
  • Author(s): 山本英司, 高師知紀, 森中洋一, 林少揚, 呉健忠, 松本隆, 北野英己, 松岡信, 芦苅基行
  • Organizer: イネ遺伝学・分子生物学ワークショップ2010
  • Place of Presentation: 名古屋大学野依記念学術交流館(愛知県)
  • Year and Date: 2010-07-12
• [Presentation] イネにおける接合後隔離機構 (2010)
  • Author(s): 山本英司
  • Organizer: 特定領域研究「植物ゲノム障壁」若手ワークショップ
  • Place of Presentation: 名古屋大学野依記念学術交流館(愛知県)
  • Year and Date: 2010-07-12
• [Patent(Industrial Property Rights)] イネの弱性判定方法 (2010)
  • Inventor(s): 高師知紀、林少揚、森中洋一、芦苅基行、山本英司
  • Industrial Property Rights Holder: 国立大学法人名古屋大学、本田技研工業株式会社
  • Filing Date: 2010-06-02
• [Patent(Industrial Property Rights)] イネの弱性判定方法 (2010)
  • Inventor(s): 高師知紀、林少揚、森中洋一、芦苅基行、山本英司
  • Industrial Property Rights Holder: 国立大学法人名古屋大学、本田技研工業株式会社
  • Industrial Property Number: 2010-127255
  • Filing Date: 2010-06-02
• [Patent(Industrial Property Rights)] 雑種弱勢遺伝子とその利用 (2010)
  • Inventor(s): 高師知紀、林少揚、森中洋一、芦苅基行、山本英司
  • Industrial Property Rights Holder: 国立大学法人名古屋大学、本田技術工業株式会社
  • Filing Date: 2010-01-29