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Production of useful compounds by means of molecular breeding of oleaginous fungi and unique microbial metabolism

Research Project

Project/Area Number 19688006
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Applied microbiology
Research InstitutionKyoto University

Principal Investigator

SAKURADANI Eiji  Kyoto University, 農学研究科, 助教 (10362427)

Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥25,740,000 (Direct Cost: ¥19,800,000、Indirect Cost: ¥5,940,000)
Fiscal Year 2009: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2008: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2007: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
Keywords発酵生産 / 分子育種 / アルカン / 高度不飽和脂肪酸 / デスモステロール / アラキドン酸 / モルティエレラ / 脂肪酸鎖長延長酵素 / 脂肪酸不飽和化酵素 / プロトテカ / ステロール
Research Abstract

Oleaginous fungus Mortierella alpina 1S-4 produces various polyunsaturated fatty acids such as arachidonic acid. The researcher worked on development of homologous recombination in this strain, functional analysis of enzyme genes involved in polyunsaturated fatty acids, PUFA production through molecular breeding, elucidation of sterol biosynthetic pathways, and study on sub-terminal oxidation mechanism of n-alkanes. The results are considered not only to be directly connected with the field of microbial lipid production but also to be important to elucidate the mechanism of microbial lipid metabolism.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (30 results)

All 2010 2009 2008 2007 Other

All Journal Article (20 results) (of which Peer Reviewed: 20 results) Presentation (9 results) Remarks (1 results)

  • [Journal Article] Overproduction of geranylgeraniol by metabolically engineered Saccharomyces cerevisiae2009

    • Author(s)
      Tokuhiro K、Muramatsu M、Ohto C、Kawaguchi T、Obata S、Muramoto N、Hirai M、Takahashi H、Kondo A、櫻谷英治、Shimizu S
    • Journal Title

      Appl. Environ. Microbiol 75(17)

      Pages: 5536-5543

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Establishment of Agrobacterium tumefaciens-mediated transformation of an oleaginous fungus, Mortierella alpina 1S-4, and its application for eicosapentaenoic acid producer breeding2009

    • Author(s)
      Ando A、Sumida Y、Negoro H、Suroto DA、Ogawa J、櫻谷英治、Shimizu S
    • Journal Title

      Appl. Environ. Microbiol 75(17)

      Pages: 5529-5535

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Alkaline pH enhances farnesol production by Saccharomyces cerevisiae2009

    • Author(s)
      Muramatsu M、Ohto C、Obata S、櫻谷英治、Shimizu S
    • Journal Title

      J. Biosci. Bioeng 108(1)

      Pages: 52-55

    • NAID

      110007359697

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Improved production of various polyunsaturated fatty acids through filamentous fungus Mortierella alpina breeding2009

    • Author(s)
      櫻谷英治、Ando A、Ogawa J、Shimizu S
    • Journal Title

      Appl. Microbiol. Biotechnol 84(1)

      Pages: 1-10

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transformation of an oleaginous zygomycete Mortierella alpina 1S-4 with the carboxin resistance gene conferred by mutation of the iron-sulfur subunit of succinate dehydrogenase2009

    • Author(s)
      Ando A、櫻谷英治、Horinaka K、Ogawa J、Shimizu S
    • Journal Title

      Curr. Genet. 55(3)

      Pages: 349-356

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Production of functional lipids by microorganisms: arachidonic acid and related polyunsaturated fatty acids2009

    • Author(s)
      櫻谷英治、Ando A、Ogawa J、Shimizu S
    • Journal Title

      Tanpakushitsu Kakusan Koso 54(6)

      Pages: 725-734

    • NAID

      40016639059

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a novel fatty acid elongase with a wide substrate specificity from arachidonic acid-producing fungus Mortierella alpina 1S-42009

    • Author(s)
      櫻谷英治、Nojiri M、Suzuki H、Shimizu S
    • Journal Title

      Appl. Microbiol. Biotechnol 84(4)

      Pages: 709-716

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Single cell oil production by Mortierella alpina2009

    • Author(s)
      櫻谷英治、Shimizu S
    • Journal Title

      J. Biotechnol 144(1)

      Pages: 31-36

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Selective production of cis-9, trans-11 isomer of conjugated linoleic acid from trans-vaccenic acid methyl ester by Delacroixia coronata2009

    • Author(s)
      Ando A、Ogawa J、Sugimoto S、Kishino S、櫻谷英治、Yokozeki K、Shimizu S
    • Journal Title

      J. Appl. Microbiol 106(5)

      Pages: 1697-1704

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of mutation sites on △12 desaturase genes from Mortierella alpina 1S-4 mutants.2009

    • Author(s)
      櫻谷英治、Abe T、Matsumura K、Tomi A、Shimizu S
    • Journal Title

      J. Biosci. Bioeng 107(2)

      Pages: 99-101

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of mutation sites on ω3 desaturase genes from Mortierella alpina 1S-4 mutants2009

    • Author(s)
      櫻谷英治、Abe T、Shimizu S
    • Journal Title

      J. Biosci. Bioeng 107(1)

      Pages: 7-9

    • NAID

      110007042018

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a novel fatty acid elongase with a wide substrate specificity from arachidonic acid-producing fungus Mortierella alpina 1S-42009

    • Author(s)
      Eiji Sakuradani
    • Journal Title

      Appl.Microbiol.Biotechnol. 84

      Pages: 709-716

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Improved production of various polyunsaturated fatty acids through filamentous fungus Mortierella alpina breeding2009

    • Author(s)
      Eiji Sakuradani
    • Journal Title

      Appl.Microbiol.Biotechnol. 84

      Pages: 1-10

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Single cell oil production by Mortierella alpina2009

    • Author(s)
      Eiji Sakuradani
    • Journal Title

      J Biotechnol 144

      Pages: 31-36

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of mutation sites on Δ12 desaturase genes from Mortierella aipina IS-4 mutants2009

    • Author(s)
      Eiii Sakuradani
    • Journal Title

      J. Biosci. Bioeng. 107

      Pages: 99-101

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of mutation sites on ω3 desaturase genes from Mortierella alpina IS-4 mutants2009

    • Author(s)
      Eiii Saktiradani
    • Journal Title

      J. Biosci. Bioeng. 107

      Pages: 7-9

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional analysis of a fatty acid elongase from arachidonic acid-producing Mortierella alpina 1S-42008

    • Author(s)
      櫻谷英治、Murata S、Kanamaru H、Shimizu S
    • Journal Title

      Appl. Microbiol. Biotechnol 81(3)

      Pages: 497-503

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional analysis of a fatty acid elongase from arachidonic acid-producing Mortierella alnina IS-42008

    • Author(s)
      Eiii Sakuradani
    • Journal Title

      Appl. Microbiol. Biotechnol. 81

      Pages: 497-503

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a sterol Δ7 reductase gene involved in desmosterol biosynthesis in Mortierella alpina 1S-42007

    • Author(s)
      Shuo Zhang
    • Journal Title

      Appl. Environ. Microbiol. 73

      Pages: 1736-1741

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a novel bifunctional Δ12/Δ15 fatty acid desaturase from a basidiomycete, Coprinus cinereus TD#822-22007

    • Author(s)
      Shuo Zhang
    • Journal Title

      FEBS Lett. 581

      Pages: 315-319

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] アラキドン酸生産性糸状菌Mortierella alpina 1S-4の脂肪酸鎖長延長酵素遺伝子の構造と機能の解明2010

    • Author(s)
      櫻谷英治、○西馬場由美、落合美佐、小川順、清水昌
    • Organizer
      2010年度日本農芸化学大会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-28
    • Related Report
      2009 Final Research Report
  • [Presentation] アラキドン酸生産性糸状菌Mortierella alpina 1S-4の脂肪酸鎖長延長酵素遺伝子のクローニングと諸性質解明2009

    • Author(s)
      櫻谷英治、野尻増俊、鈴木春奈、清水昌
    • Organizer
      2009年度合同沖縄大会(日本農芸化学会)
    • Place of Presentation
      沖縄
    • Year and Date
      2009-10-31
    • Related Report
      2009 Final Research Report
  • [Presentation] 油糧微生物Mortierella alpinaより取得したケトコナゾール耐性変異株の諸性質解明2009

    • Author(s)
      福岡義宏、櫻谷英治、清水昌
    • Organizer
      2009年度合同沖縄大会(日本農芸化学会)
    • Place of Presentation
      沖縄
    • Year and Date
      2009-10-31
    • Related Report
      2009 Final Research Report
  • [Presentation] 油糧微生物による有用脂質生産と特異な脂質変換反応の探索2009

    • Author(s)
      櫻谷英治、安藤晃規、小川順、清水昌
    • Organizer
      第61回日本生物工学会大会
    • Place of Presentation
      愛知県(名古屋)
    • Year and Date
      2009-09-25
    • Related Report
      2009 Final Research Report
  • [Presentation] 油糧微生物による有用脂質生産と特異な脂質変換反応の探索2009

    • Author(s)
      櫻谷英治
    • Organizer
      第61回 日本生物工学会大会
    • Place of Presentation
      名古屋大学東山キャンパス(愛知県)
    • Year and Date
      2009-09-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] アラキドン酸生産性糸状菌Mortierella alpina 1S-4の分子育種による有用脂質生産2009

    • Author(s)
      櫻谷英治、安藤晃規、小川順、清水昌
    • Organizer
      2009年度日本農芸化学大会
    • Place of Presentation
      福岡県(博多)
    • Year and Date
      2009-03-28
    • Related Report
      2009 Final Research Report
  • [Presentation] アラキドン酸生産性糸状菌物Mortierella alpinaの分子育種による有用脂質生産2009

    • Author(s)
      櫻谷英治
    • Organizer
      日本農芸化学会
    • Place of Presentation
      福岡県(博多)
    • Year and Date
      2009-03-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] 油糧微生物の代謝工学と機能性脂質生産への利用2008

    • Author(s)
      櫻谷英治、岸野重信、小川順、横関健三、○清水昌
    • Organizer
      第60回日本生物工学会大会
    • Place of Presentation
      宮城(仙台)
    • Year and Date
      2008-08-29
    • Related Report
      2009 Final Research Report
  • [Presentation] 微細藻Pavlova sp.の脂肪酸鎖長延長酵素遺伝子の油糧微生物Mortierella alpina 1S-4での発現2007

    • Author(s)
      伊東 久仁
    • Organizer
      日本生物工学会
    • Place of Presentation
      広島大学東広島キャンパス総合科学部
    • Year and Date
      2007-09-27
    • Related Report
      2007 Annual Research Report
  • [Remarks]

    • URL

      http://www.hakko.kais.kyoto-u.ac.jp/lab-e/index-e.html

    • Related Report
      2009 Final Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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