| Budget Amount *help |
¥20,930,000 (Direct Cost: ¥16,100,000、Indirect Cost: ¥4,830,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000)
Fiscal Year 2007: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
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| Research Abstract |
A plankton DNA database and monitoring method were developed in this project. Plankton cultures were isolated from Lake Biwa, and 18S or 16S rRNA genes were amplified with each universal primer set. The precise sequence of nucleotides in a sample of SSU-rRNA gene was analyzed after TA cloning treatment. Primers for real-time PCR to detect 18 target species from Lake Biwa were designed by Primer 3 software based on the alignment of the Lake Biwa Plankton DNA Database using DNASIS Pro V2.10. These primer sets were tested using a real time PCR system, and the results of 15 species' detection were synchronized using a microscope. PCR-DGGE (Denaturing Gradient Gel Electrophoresis) analysis successfully showed pico-plankton species that have not been previously detected in Lake Biwa. We believe that these molecular methods will be useful for future diversity studies as well as for long-term plankton monitoring.
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