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Self-renewal mechanism of spermatogonial stem cells

Research Project

Project/Area Number 19689007
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field General medical chemistry
Research InstitutionKyoto University

Principal Investigator

SHINOHARA Mito  Kyoto University, 医学研究科, 助教 (10372591)

Project Period (FY) 2007 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥25,350,000 (Direct Cost: ¥19,500,000、Indirect Cost: ¥5,850,000)
Fiscal Year 2010: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2009: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2008: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2007: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Keywords幹細胞 / 生殖 / 遺伝学 / 発生・分化 / がん / 細胞・組織 / 移植 再生医療 / バイオテクノロジー / 発生 分化
Research Abstract

Spermatogonial stem cells (SSCs) in testes are the foundation of spermatogenesis throughout adult life. In 2003, we established a long-term in vitro culture system of mouse SSCs, and designated the cultured cells as Germline stem (GS) cells. GS cells constantly proliferate for a long-term period and expand for 10^<85>-fold (139 passages)during 2 years culture, without losing stem cell activity, normal imprinting patterns, and normal karyotype. Current research was oriented to elucidate the limitation of SSC stability, and the mechanism underlying a stability self-renewal system of SSCs, and how the breakdown of safety mechanism occurs.
First, we continued to culture GS cells beyond 2 years, and examined their life-span, and observed the effects of a long-term culture. We found that GS cells continue to proliferate for >5 years, without losing normal karyotype, but their stem cell activity and differentiation potential became gradually limited by a long-term culture.
Second, we investigated the signaling pathways regulating SSC self-renewal, and found that the signal from Glial cell line derived neurotrophic factor (GDNF), the self-renewal factor of SSCs, is transduced through Akt/PI3K pathway, and that Ras/Cyclin D2 pathway is also involved in self-renewal of SSCs. In addition, we found that p21 and p27,Cyclin-dependent kinase inhibitor (CDKI)are involved in self-renewal and differentiation of SSCs.

Report

(6 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report   Self-evaluation Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (86 results)

All 2011 2010 2009 2008 2007 Other

All Journal Article (59 results) (of which Peer Reviewed: 25 results) Presentation (26 results) Book (1 results)

  • [Journal Article] Unstable side population phenotype of mouse spermatogonial stem cells in vitro.2011

    • Author(s)
      Shinohara T., Ishii K., Kanatsu-Shinohara M.
    • Journal Title

      J.Reprod.Dev. In press

    • Related Report
      2010 Final Research Report
  • [Journal Article] Homologous recombination in rat germline stem cells.2011

    • Author(s)
      Kanatsu-Shinohara M., Kato-Itoh M., Ikawa M., Takehashi M., Sanbo M., Morioka Y., Tanaka T., Morimoto H., Hirabayashi M., Shinohara T.
    • Journal Title

      Biol.Reprod.

      Volume: (In press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Unstable side population phenotype of mouse spermatogonial stem cells in vitro.2011

    • Author(s)
      Shinohara T., Ishii K., Kanatsu-Shinohara M.
    • Journal Title

      J.Reprod.Dev.

      Volume: (In press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Serum- and feeder-free culture of mouse germline stem cells.2011

    • Author(s)
      Kanatsu-Shinohara M., Inoue K., Ogonuki N., Morimoto H., Ogura A., Shinohara T.
    • Journal Title

      Biol.Reprod.

      Volume: 84 Pages: 97-105

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Serum- and feeder-free culture of mouse germline stem cells.2010

    • Author(s)
      Kanatsu-Shinohara M., Inoue K., Ogonuki N., Morimoto H., Ogura A., Shinohara T.
    • Journal Title

      Biol.Reprod. 84(1)

      Pages: 97-105

    • Related Report
      2010 Final Research Report
  • [Journal Article] Germline modification using mouse spermatogonial stem cells.2010

    • Author(s)
      Kanatsu-Shinohara M., Shinohara T.
    • Journal Title

      Methods Enzymol. 477

      Pages: 17-36

    • Related Report
      2010 Final Research Report
  • [Journal Article] Kanatsu-Shinohara M., Shinohara T., Nakahata T., Heike T.Neonatal mouse testis-derived multipotent germline stem cells improve the cardiac function of acute ischemic heart mouse model.2010

    • Author(s)
      Iwasa T., BabaS., Doi H., Kaichi S., Yokoo N., Miwa T.
    • Journal Title

      Biochem.Biophys.Res.Commun. 400(1)

      Pages: 27-33

    • Related Report
      2010 Final Research Report
  • [Journal Article] Transmission distortion by loss of p21 or p27 cyclin-dependent kinase inhibitors following competitive spermatogonial transplantation.2010

    • Author(s)
      Kanatsu-Shinohara M., Takashima S., Shinohara T.
    • Journal Title

      Proc.Natl.Acad.Sci.USA 107(14)

      Pages: 6210-6215

    • Related Report
      2010 Final Research Report
  • [Journal Article] Genetic influences in mouse spermatogonial stem cell self-renewal.2010

    • Author(s)
      Kanatsu-Shinohara M., Ogonuki N., Miki H., Inoue K., Morimoto H., Takashima S., Ogura A., Shinohara T.
    • Journal Title

      J.Reprod.Dev. 56(1)

      Pages: 145-53

    • Related Report
      2010 Final Research Report
  • [Journal Article] Neonatal mouse testis-derived multipotent germline stem cells improve the cardiac function of acute ischemic heart mouse model2010

    • Author(s)
      Iwasa T., Baba S., Doi H., Kaichi S., Yokoo N., Miwa T., Kanatsu-Shinohara M., Shinohara T., Nakahata T., Heike T
    • Journal Title

      Biochem.Biophys.Res.Commun.

      Volume: 400 Pages: 27-33

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Germline modification using mouse spermatogonial stem cells.2010

    • Author(s)
      Kanatsu-Shinohara M., Shinohara T.
    • Journal Title

      Methods Enzymol.

      Volume: 477 Pages: 17-36

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transmission distortion by loss of p21 or p27 cyclin-dependent kinase inhibitors following competitive spermatogonial transplantation.2010

    • Author(s)
      Kanatsu-Shinohara M., Takashima S., Shinohara T.
    • Journal Title

      Proc. Natl. Acad. Sci. USA 107(14)

      Pages: 6210-6215

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Genetic influences in mouse spermatogonial stem cell self-renewal.2010

    • Author(s)
      Kanatsu-Shinohara M., Ogonuki N., Miki H., Inoue K., Morimoto H., Takashima S., Ogura A., Shinohara T.
    • Journal Title

      J. Reprod. Dev. 56(1)

      Pages: 145-153

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Transmission distortion by loss of p21 or p27 cyclin-dependent kinase inhibitors following competitive spermatogonial transplantation.2010

    • Author(s)
      Kanatsu-Shinohara M., Takashima S., Shinohara T.
    • Journal Title

      Proc.Natl.Acad.Sci.USA (In press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetic influences in mouse spermatogonial stem cell self-renewal.2010

    • Author(s)
      Kanatsu-Shinohara M., Ogonuki N., Miki H., Inoue K., Morimoto H., Takashima S.Ogura A., Shinohara T.
    • Journal Title

      J.Reprod.Dev. 56(1)

      Pages: 145-153

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phenotypic plasticity of mouse spermatogonial stem cells.2009

    • Author(s)
      Morimoto H., Kanatsu-Shionohara M., Takashima S., Chuma S., Nakatsuji N., Shinohara T.
    • Journal Title

      PLoS One. 4(11)

    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Journal Article] Genetic reconstitution of mouse spermatogonial stem cell self-renewal in vitro by Ras/cyclin D2 activation.2009

    • Author(s)
      Lee J., Kanatsu-Shinohara M., Morimoto H., Kazuki Y., Takashima S., Oshimura M., Toyokuni S., Shinohara T.
    • Journal Title

      Cell Stem Cell 5(1)

      Pages: 76-86

    • Related Report
      2010 Final Research Report 2009 Self-evaluation Report
  • [Journal Article] Abnormal DNA methyltransferase expression in mouse germline stem cells results in spermatogenic defects.2009

    • Author(s)
      Takashima S., Takehashi M., Lee J., Chuma S., Okano M., Hata K., Suetake I., Nakatsuji N., Miyoshi H., Tajima S., Tanaka Y., Toyokuni S., Sasaki H., Kanatsu-Shinohara M., Shinohara T.
    • Journal Title

      Biol.Reprod. 81(1)

      Pages: 64-155

    • Related Report
      2010 Final Research Report
  • [Journal Article] Heritable imprinting defect caused by epigenetic abnormalities in mouse spermatogonial stem cells.2009

    • Author(s)
      Lee J., Kanatsu-Shinohara M., Ogonuki N., Miki H., Inoue K., Morimoto H., Ogura A., Shinohara T.
    • Journal Title

      Biol.Reprod. 80(3)

      Pages: 27-528

    • Related Report
      2010 Final Research Report
  • [Journal Article] Phenotypic plasticity of mouse spermatogonial stem cells.2009

    • Author(s)
      Morimoto H., Kanatsu-Shionohara M., Takashima S., Chuma S., Nakatsuji N., Shinohara T.
    • Journal Title

      PLoS One. 4(11),e7909

      Pages: 1-9

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Abnormal DNA methyltransferase expression in mouse germline stem cells results in spermatogenic defects.2009

    • Author(s)
      Takashima S., Takehashi M., Lee J., Chuma S., Okano M., Hata K., Suetake I., Nakatsuji N., Miyoshi H., Tajima S., Tanaka Y., Toyokuni S., Sasaki H., Kanatsu-Shinohara M., Shinohara T.
    • Journal Title

      Biol. Reprod. 81(1)

      Pages: 155-164

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Heritable imprinting defect caused by epigenetic abnormalities in mouse spermatogonial stem cells.2009

    • Author(s)
      Lee J., Kanatsu-Shinohara M., Ogonuki N., Miki H., Inoue K., Morimoto H., Ogura A., Shinohara T.
    • Journal Title

      Biol. Reprod. 80(3)

      Pages: 518-527

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Bone marrow engraftment but limited expansion of hematopoietic cells from multipotent germline stem cells derived from neonatal mouse testis.2009

    • Author(s)
      Yoshimoto M., Heike T., Chang H., Kanatsu-Shinohara M., Baba S., Varnau J.T., Shinohara T., Yoder M, C., Nakahata T.
    • Journal Title

      Exp.Hematol. 37(12)

      Pages: 1400-1410

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetic reconstitution of mouse spermatogonial stem cell self-renewal in vitro by Ras/cyclin D2 activation.2009

    • Author(s)
      Lee J., Kanatsu-Shinohara M., Morimoto H., Kazuki Y.Takashima S., Oshimura M., Toyokuni S., Shinohara T.
    • Journal Title

      Cell Stem Cell 5(1)

      Pages: 76-86

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Large-scale production of growing oocytes in vitro from neonatal mouse ovaries.2009

    • Author(s)
      Honda A., Hirose M., Inoue K., Hiura H., Miki H., Ogonuki N., Sugimoto M., Abe K., Kanatsu-Shinohara M., Kono T., Shinohara T., Ogura A.
    • Journal Title

      Int.J., Dev.Biol. 53(4)

      Pages: 605-613

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Heritable imprinting defect caused by epigenetic abnormalities in mouse spermatogonial stem cells2009

    • Author(s)
      Lee J. et.al.
    • Journal Title

      Biol. Reprod. 80

      Pages: 518-27

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Brief history, pitfalls, and prospects of mammalian spermatogonial stem cell research.Cold Spring Harb.2008

    • Author(s)
      Kanatsu-Shinohara M., Takehashi M., Shinohara T.
    • Journal Title

      Symp.Quant.Biol. 73

      Pages: 17-23

    • Related Report
      2010 Final Research Report
  • [Journal Article] Homing of mouse spermatogonial stem cells to germline niche depends on b1-integrin.2008

    • Author(s)
      Kanatsu-Shinohara M., Takehashi M., Takashima S., Lee J., Morimoto H., Chuma S.,Raducanu A., Nakatsuji N., Fassler R., Shinohara T.
    • Journal Title

      Cell Stem Cell 3(5)

      Pages: 42-533

    • Related Report
      2010 Final Research Report
  • [Journal Article] Production of transgenic rats following lentiviral transduction and xenogeneic transplantation of spermatogonial stem cells.2008

    • Author(s)
      Kanatsu-Shinohara M., Kato M., Takehashi M., Hirabayashi M., Shinohara T.
    • Journal Title

      Biol.Reprod. 79(6)

      Pages: 8-1121

    • Related Report
      2010 Final Research Report
  • [Journal Article] Long-term culture of male germlinestem cells from hamster testes.2008

    • Author(s)
      Kanatsu-Shinohara M., Muneto T., Lee J., Takenaka., Chuma S, Nakatsuji N., Horiuchi T., Shinohara T.
    • Journal Title

      Biol.Reprod. 78(4)

      Pages: 7-611

    • Related Report
      2010 Final Research Report
  • [Journal Article] Pluripotency of a single spermatogonial stem cell in mice.2008

    • Author(s)
      Kanatsu-Shinohara M., Lee J., Inoue K., Ogonuki N., Miki H., Toyokuni S.,Ikawa M., Nakamura T., Ogura A., Shinohara T.
    • Journal Title

      Biol.Reprod. 78(4)

      Pages: 7-81

    • Related Report
      2010 Final Research Report
  • [Journal Article] Brief history, pitfalls, and prospects of mammalian spermatogonial stem cell research.2008

    • Author(s)
      Kanatsu-Shinohara M., Takehashi M., Shinohara T.
    • Journal Title

      Cold Spring Harb. Symp. Quant. Biol. 73

      Pages: 17-23

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Homing of mouse spermatogonial stem cells to germline niche depends on b1-integrin.2008

    • Author(s)
      Kanatsu-Shinohara M., Takehashi M., Takashima S., Lee J., Morimoto H., Chuma S., Raducanu A., Nakatsuji N., Fassler R., Shinohara T.
    • Journal Title

      Cell Stem Cell 3(5)

      Pages: 533-542

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Production of transgenic rats following lentiviral transduction and xenogeneic transplantation of spermatogonial stem cells.2008

    • Author(s)
      Kanatsu-Shinohara M., Kato M., Takehashi M., Hirabayashi M., Shinohara T.
    • Journal Title

      Biol. Reprod. 79(6)

      Pages: 1121-1128

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Long-term culture of male germlinestem cells from hamster testes.2008

    • Author(s)
      Kanatsu-Shinohara M., Muneto T., Lee J., Takenaka., Chuma S, Nakatsuji N., Horiuchi T., Shinohara T.
    • Journal Title

      Biol. Reprod. 78(4)

      Pages: 611-617

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Pluripotency of a single spermatogonial stem cell in mice.2008

    • Author(s)
      Kanatsu-Shinohara M., Lee J., Inoue K., Ogonuki N., Miki H., Toyokuni S., Ikawa M., Nakamura T., Ogura A., Shinohara T.
    • Journal Title

      Biol. Reprod. 78(4)

      Pages: 681-687

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Production of transgenic rats following lentiviral transduction and xenogeneic transplantation of spermatdgonial stem cells2008

    • Author(s)
      Kanatsu-Shinohara M. et.al.
    • Journal Title

      Biol. Reprod. 79

      Pages: 1121-8

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Homing of mouse spermatogonial stem cells to germline nichedepends on β1-integrin2008

    • Author(s)
      Kanatsu-Shinohara M. et.al.
    • Journal Title

      Cell Stem Cell 3

      Pages: 533-42

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Correction of a genetic defect in muitipotent germline stemcells using a human artificial chromosome2008

    • Author(s)
      Kazuki Y., et.al.
    • Journal Title

      Gene Ther. 15

      Pages: 617-24

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Long-term culture of male germline stem cells from hamster testes2008

    • Author(s)
      Kanatsu-Shinohara M. et.al.
    • Journal Title

      Biol. Reprod 78

      Pages: 611-7

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pluripotency of a single spermatogonial stem cell in mice2008

    • Author(s)
      Kanatsu-Shinohara M. et.al.
    • Journal Title

      Biol. Reprod. 78

      Pages: 681-7

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Correction of a genetic defect in multipotent germline stem cells using a human artificial chromosome.2008

    • Author(s)
      Kazuki Y., et. al.
    • Journal Title

      Gene Ther. (In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pluripotency of a single spermatogonial stem cell in mice.2008

    • Author(s)
      Kanatsu-Shinohara M., et. al.
    • Journal Title

      Biol. Reprod. (In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Long-term culture of male germline stem cells from hamster testes.2008

    • Author(s)
      Kanatsu-Shinohara M., et. al.
    • Journal Title

      Biol. Reprod. (In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Production of knockout mice by gene targeting in multipotent germline stem cells.2007

    • Author(s)
      Takehashi M, Kanatsu-Shinohara M, Miki H, Lee J, Kazuki Y, Inoue K, Ogonuki N, Toyokuni S, Oshimura M, Ogura A, Shinohara, T.
    • Journal Title

      Dev.Biol. 312

      Pages: 52-344

    • Related Report
      2010 Final Research Report
  • [Journal Article] Culture and genetic modification of mouse germline stem cells.2007

    • Author(s)
      Kanatu-Shinohara M., Shinohara T.
    • Journal Title

      Ann.N.Y.Acad.Sci. 312(1)

      Pages: 344-52

    • Related Report
      2010 Final Research Report
  • [Journal Article] Akt mediates self-renewal division of mouse spermatogonial stem cells.2007

    • Author(s)
      Lee J., Kanatsu-Shinohara M., Inoue K., Ogonuki N., Miki H., Toyokuni S., Kimura T., Nakano T., Ogura A., Shinohara T.
    • Journal Title

      Development 134(10)

      Pages: 9-1853

    • Related Report
      2010 Final Research Report 2009 Self-evaluation Report
  • [Journal Article] Adenovirus-mediated gene delivery into mouse spermatogonial stem cells.2007

    • Author(s)
      Takehashi M., Kanatsu-Shinohara M., Inoue K., Ogonuki N., Miki H., Toyokuni S., Ogura A., Shinohara T.
    • Journal Title

      Proc.Natl.Acad.Sci.USA 104(8)

      Pages: 2596-601

    • Related Report
      2010 Final Research Report
  • [Journal Article] Production of knockout mice by gene targeting in multipotent germline stem cells.2007

    • Author(s)
      Takehashi M, Kanatsu-Shinohara M, Miki H, Lee J, Kazuki Y, Inoue K, Ogonuki N, Toyokuni S, Oshimura M, Ogura A, Shinohara, T.
    • Journal Title

      Dev. Biol. 312

      Pages: 344-352

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Culture and genetic modification of mouse germline stem cells.2007

    • Author(s)
      Kanatu-Shinohara M., Shinohara T.
    • Journal Title

      Ann. N.Y. Acad. Sci. 312(1)

      Pages: 344-352

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Adenovirus-mediated gene delivery into mouse spermatogonial stem cells.2007

    • Author(s)
      Takehashi M., Kanatsu-Shinohara M., Inoue K., Ogonuki N., Miki H., Toyokuni S., Ogura A., Shinohara T.
    • Journal Title

      Proc. Natl. Acad. Sci. USA 104(8)

      Pages: 2596-2601

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Production of knockout mice by gene targeting in multipotent sermline stem cells.2007

    • Author(s)
      Takehashi M., et. al.
    • Journal Title

      Dev. Biol. 312

      Pages: 344-52

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Culture and genetic modification of mouse germline stem cells.2007

    • Author(s)
      Kanatsu-Shinohara M., Shinohara T.
    • Journal Title

      Ann. N. Y. Acad. Sci. 1120

      Pages: 59-71

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Isolation, characterization, and in vitro differentiation of putative thecal stem cells.2007

    • Author(s)
      Honda A., et. al.
    • Journal Title

      Proc. Natl. Acad. Sci. USA 104

      Pages: 12389-94

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Akt mediates self-renewal division of mouse spermatogonial stem cells.2007

    • Author(s)
      Lee J., et. al.
    • Journal Title

      Development 134

      Pages: 1853-9

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Homologous recombination in rat germline stem cells.

    • Author(s)
      Kanatsu-Shinohara M., Kato-Itoh M., Ikawa M., Takehashi M., Sanbo M., Morioka Y., Tanaka T., Morimoto H., Hirabayashi M., Shinohara T.
    • Journal Title

      Biol.Reprod. In press

    • Related Report
      2010 Final Research Report
  • [Journal Article] Large-scale production of growing oocytes in vitro from neonatal mouse ovaries

    • Author(s)
      Honda A., et.al.
    • Journal Title

      Int. J., Dev. Biol. (In press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Abnormal DNA methyltransferase expression in mouse germline stem cells results in spermatogenic defects

    • Author(s)
      Takashima S. et.al.
    • Journal Title

      Biol. Reprod. (In press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Brief history, pitfalls, and prospects of mammalian spermatogonial stem cell research

    • Author(s)
      Kanatsu-Shinohara M. et.al.
    • Journal Title

      Cold Spring Harb. Symp. Quant. Biol. (In press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] 生殖細胞特異的な遺伝情報場の継承のメカニズムの解明2010

    • Author(s)
      篠原美都
    • Organizer
      アステラス病態代謝研究会 第41回研究報告会
    • Place of Presentation
      東京・経団連会館
    • Year and Date
      2010-10-16
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] Positive and negative regulators ofmouse spermatogonial stem cellself-renewal2010

    • Author(s)
      Takashi Shnohara,Seiji Takashima,MitoKanatsu-Shinohara
    • Organizer
      11^th International Symposium on Spematology
    • Place of Presentation
      沖縄コンベンションセンター
    • Year and Date
      2010-06-26
    • Related Report
      2010 Final Research Report
  • [Presentation] Positive and negative regulators of mouse spermatogonial stem cell self-renewal2010

    • Author(s)
      Takashi Shinohara, Seiji Takashima, Mito, Kanatsu-Shinohara
    • Organizer
      11^<th> International Symposium on Spematology
    • Place of Presentation
      沖縄コンベンションセンター
    • Year and Date
      2010-06-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] CDKインヒビターp21とp27 による精子幹細胞の制御2010

    • Author(s)
      篠原美都
    • Organizer
      新学術領域第三回領域会議
    • Place of Presentation
      筑波大学附属下田臨海実験センター
    • Related Report
      2010 Final Research Report
  • [Presentation] 精子幹細胞の制御におけるCDKインヒビターp21とp27の関与2010

    • Author(s)
      篠原美都
    • Organizer
      特定領域研究班会議
    • Place of Presentation
      門司港ホテル
    • Related Report
      2010 Final Research Report
  • [Presentation] マウス精子幹細胞ニッシェ制御におけるG蛋白質分子の役割の解明2010

    • Author(s)
      篠原美都
    • Organizer
      新学術領域研究「配偶子制御」第4回領域会議
    • Place of Presentation
      葉山・IPC生産性国際交流センター
    • Related Report
      2010 Final Research Report
  • [Presentation] CDKインヒビターp21とp27による精子幹細胞の制御2010

    • Author(s)
      篠原美都
    • Organizer
      新学術領域「配偶子制御」第三回領域会議
    • Place of Presentation
      筑波大学附属下田臨海実験センター
    • Related Report
      2010 Annual Research Report
  • [Presentation] 精子幹細胞の制御におけるCDKインヒビターp21とp27の関与2010

    • Author(s)
      篠原美都
    • Organizer
      特定領域研究「細胞増殖制御」班会議
    • Place of Presentation
      門司港ホテル
    • Related Report
      2010 Annual Research Report
  • [Presentation] マウス精子幹細胞ニッシェ制御におけるG蛋白質分子の役割の解明2010

    • Author(s)
      篠原美都
    • Organizer
      新学術領域研究「配偶子制御」第4回領域会議
    • Place of Presentation
      葉山・IPC生産性国際交流センタ
    • Related Report
      2010 Annual Research Report
  • [Presentation] 試験管内で発生した精子幹細胞がもたらす世代を超えて伝達するエピジェネティック異常2009

    • Author(s)
      篠原美都
    • Organizer
      特定領域「細胞外領域」第二回班会議京都大学
    • Place of Presentation
      芝蘭会館
    • Related Report
      2010 Final Research Report
  • [Presentation] 精子幹細胞の細胞周期の調節機構の解明2009

    • Author(s)
      篠原美都
    • Organizer
      特定領域「細胞増殖制御」班会議
    • Place of Presentation
      ホテルアンビエント安曇野
    • Related Report
      2010 Final Research Report
  • [Presentation] 精子幹細胞の癌化における細胞外環境の関わり2009

    • Author(s)
      篠原美都
    • Organizer
      特定領域「細胞外環境」班会議
    • Place of Presentation
      京都国際会議場
    • Related Report
      2010 Final Research Report
  • [Presentation] 精子幹細胞の自己複製制御機構2009

    • Author(s)
      篠原美都
    • Organizer
      新学術領域研究「配偶子制御」第2回領域会議
    • Place of Presentation
      総合研究大学
    • Related Report
      2010 Final Research Report
  • [Presentation] 精子幹細胞を用いた神経疾患モデルラット作成技術の開発2009

    • Author(s)
      篠原美都
    • Organizer
      特定領域「統合脳」班会議
    • Place of Presentation
      東京・学士会館
    • Related Report
      2010 Final Research Report
  • [Presentation] 試験管内で発生した精子幹細胞がもたらす世代を超えて伝達するエピジェネティック異常2009

    • Author(s)
      篠原美都
    • Organizer
      特定領域「細胞外領域」第二回班会議
    • Place of Presentation
      京都大学芝蘭会館
    • Related Report
      2008 Annual Research Report
  • [Presentation] 精子幹細胞を用いた神経疾患モデルラット作成技術の開発2008

    • Author(s)
      篠原美都
    • Organizer
      特定領域研究「統合脳」5領域合同班会議北海道厚生年金会館
    • Place of Presentation
      ウェルシティ札幌
    • Related Report
      2010 Final Research Report
  • [Presentation] 精子幹細胞のニッシェの構成分子の解明2008

    • Author(s)
      篠原美都
    • Organizer
      特定領域研究「細胞の運命と挙動を支配する細胞外環境のダイナミズム」班会議
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2010 Final Research Report
  • [Presentation] 精子幹細胞の細胞周期の調節機構の解明2008

    • Author(s)
      篠原美都
    • Organizer
      特定領域研究「細胞増殖制御」班会議
    • Place of Presentation
      御殿場高原ホテル
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] 精子幹細胞を用いた神経疾患モデルラット作成技術の開発2008

    • Author(s)
      篠原美都
    • Organizer
      特定領域研究「統合脳」5領域合同班会議
    • Place of Presentation
      北海道厚生年金会館(ウェルシティ札幌)
    • Related Report
      2008 Annual Research Report
  • [Presentation] 精子幹細胞のニッシェの構成分子の解明2008

    • Author(s)
      篠原美都
    • Organizer
      特定領域研究「細胞の運命と挙動を支配する細胞外環境のダナミズム」班会議
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2008 Annual Research Report
  • [Presentation] 精子幹細胞による遺伝子改変動物の作成」生殖科学奨励賞受賞2007

    • Author(s)
      篠原美都
    • Organizer
      日本繁殖生物学会第100回記念シンポジウム
    • Place of Presentation
      東京大学弥生講堂
    • Year and Date
      2007-10-22
    • Related Report
      2010 Final Research Report
  • [Presentation] 精子幹細胞による遺伝子改変動物の作成2007

    • Author(s)
      篠原美都
    • Organizer
      日本繁殖生物学会 第100回記念大会
    • Place of Presentation
      東京大学弥生講堂
    • Year and Date
      2007-10-22
    • Related Report
      2007 Annual Research Report
  • [Presentation] 精子幹細胞のニッシェへのホーミングに関与する分子機構の解明2007

    • Author(s)
      篠原美都
    • Organizer
      特定領域研究「細胞の運命と挙動を支配する細胞外環境のダイナミズム」班会議
    • Place of Presentation
      京都国際会議場
    • Year and Date
      2007-07-26
    • Related Report
      2010 Final Research Report 2007 Annual Research Report
  • [Presentation] 精子幹細胞の機能とその技術開発2007

    • Author(s)
      篠原美都
    • Organizer
      特定領域研究「幹細胞の可塑性と未分化性維持機構」公開班会議
    • Place of Presentation
      東京大学医科学研究所講堂
    • Related Report
      2010 Final Research Report
  • [Presentation] 精子幹細胞の多分化能制御に関わる遺伝子デコードプログラムの解明2007

    • Author(s)
      篠原美都
    • Organizer
      特定領域研究「遺伝情報発現におけるDECODEシステムの解明」合同班会議
    • Place of Presentation
      箱根湯本温泉ホテルおかだ
    • Related Report
      2010 Final Research Report
  • [Presentation] 精子幹細胞の多分化能制御に関わる遺伝子デコードプログラムの解明2007

    • Author(s)
      篠原美都
    • Organizer
      特定領域研究「遺伝情報発現におけるDECODEシステムの解明」合同班会議
    • Place of Presentation
      箱根湯本温泉 ホテルおかだ
    • Related Report
      2007 Annual Research Report
  • [Book] seriesモデル動物利用マニュアル「生物機能モデルと新しいリソース・リサーチツール」2011

    • Author(s)
      篠原隆司・篠原美都
    • Total Pages
      7
    • Publisher
      株式会社エル・アイ・シー
    • Related Report
      2010 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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