Translational research of ocular circulation for new treatment of diabetic retinopathy

• Project/Area Number: 19689035
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Ophthalmology
• Research Institution: Asahikawa Medical College
• Principal Investigator: NAGAOKA Taiji Asahikawa Medical College, 医学部, 講師 (00333691)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000); Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2007: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
• Keywords: 糖尿病網膜症 / 微小循環 / 血管内皮機能 / トランスレーショナルリサーチ / 一酸化窒素

Research Abstract

眼循環のトランスレーショナルリサーチを実践すべく基礎および臨床研究を行った。まず、旭川医科大学にブタ摘出血管を用いたin vitro実験系を国内で初めて構築し、網膜血管への薬物の直接作用とメカニズムについて検討した。また、ネコを用いたin vivo生体実験系では、非侵襲的に網膜血管内皮機能を評価できる可能性を示した。臨床研究では、2型糖尿病患者の眼循環動態についての詳しい検討を行った。

Research Products

• [Journal Article] Effect of Panretinal Photocoagulation on Choroidal Circulation in the Foveal Region in Patients with Severe Diabetic Retinopathy (2008)
  • Author(s): Takahashi A, Nagaoka T, Sato E, Yoshida A
  • Journal Title: Br J Ophthalmol 92 Pages: 1369-73
  • Peer Reviewed
• [Journal Article] Role of Nitric Oxide in Regulation of Retinal Blood Flow in Response to Hyperoxia in Cats (2008)
  • Author(s): Izumi N, Nagaoka T, Sato E, Sogawa K, Kagokawa H, Takahashi A, Kawahara A, Yoshida A
  • Journal Title: Invest Ophthalmol Vis Sci. 49 Pages: 4595-603
  • Peer Reviewed
• [Journal Article] Short-term effects of topical tafluprost on retinal blood flow in cats (2008)
  • Author(s): Izumi N, Nagaoka T, Sato E, Mori F, Takahashi A, Sogawa K, Yoshida A
  • Journal Title: Journal of Ocular Pharmacology & Therapeutics 24(5) Pages: 521-526
  • Peer Reviewed
• [Journal Article] Role of prorenin in the pathogenesis of retinal neovascularization (2008)
  • Author(s): Yokota H, Takamiya A, Nagaoka T, Hikichi T, Ishida Y, Suzuki F, Yoshida A
  • Journal Title: Hokkaido Igaku Zasshi 83(3) Pages: 159-65
  • NAID: 10029922738
  • Peer Reviewed
• [Journal Article] C-Reactive Protein Inhibits Endothelium-Dependent Nitric Oxide-Mediated Dilation of Retinal Arterioles via Enhanced Superoxide Production (2008)
  • Author(s): Nagaoka T, Kuo L, Yoshida A, Hein T
  • Journal Title: Invest Ophthalmol Vis Sci 49 Pages: 2053-60
  • Peer Reviewed
• [Journal Article] Effect of Panretinal Photocoagulation on Choroidal Circulation in the Foveal Region in Patients with Severe Diabetic Retinopathy (2008)
  • Author(s): Takahashi A, Nagaoka T, Sato E, Yoshida A.
  • Journal Title: British Journal of Ophthalmology 92 Pages: 1369-1373
  • Peer Reviewed
• [Journal Article] Role of Nitric Oxide in Regulation of Retinal Blood Flow in Response to Hvneroxia in Cats (2008)
  • Author(s): Izumi N, Nagaoka T, Sato E, et.al.
  • Journal Title: Investigative Ophthalmollogy & Visual Science 49 Pages: 4595-4603
  • Peer Reviewed
• [Journal Article] Short-term effects of topical tafluprost on retinal blood flow in cats (2008)
  • Author(s): Izumi N, Nagaoka T, Sato E, et.al.
  • Journal Title: Journal of OcuIar Pharmacology & Therapeutics 24 Pages: 521-526
  • Peer Reviewed
• [Journal Article] C-Reactive Protein Inhibits Endothelium-Dependent Nitric Oxide-Mediated Dilation of Retinal Arterioles via Enhanced Suneroxide Production (2008)
  • Author(s): Nagaoka T, Kuo L, Yoshida A et.al.
  • Journal Title: Investigative Ophthalmollogy & Visual Science 49 Pages: 2053-2060
  • Peer Reviewed
• [Journal Article] Role of prorenin in the pathogenesis of retinal neovascularization (2008)
  • Author(s): Yokota H, Takamiya A, Nagaoka T
  • Journal Title: Hokkaido Igaku Zasshi 83 Pages: 159-165
  • NAID: 10029922738
  • Peer Reviewed
• [Journal Article] Resveratrol, a Component of Red Wine, Elicits Dilation of Isolated Porcine Retinal Arterioles: Role of Nitric Oxide and Potassium Channels (2007)
  • Author(s): Nagaoka T, Hein T, Yoshida A, Kuo L
  • Journal Title: Invest Ophthalmol Vis Sci 2007 48(9) Pages: 4232-4239
  • Peer Reviewed
• [Journal Article] Prorenin levels in retinopathy of prematurity (2007)
  • Author(s): Yokota H, Nagaoka T, Mori F, Hikichi T, Hosokawa H, Tanaka H, Ishida Y, Suzuki F, Yoshida A
  • Journal Title: Am J Ophthalmol 143(3) Pages: 531-3
  • Peer Reviewed
• [Journal Article] Simvastatin Elicits Dilation of Isolated Porcine Retinal Arterioles: Role of Nitric Oxide and Mevalonate-Rho Kinase Pathway (2007)
  • Author(s): Nagaoka T, Hein T, Yoshida A, Kuo L
  • Journal Title: Invest Ophthalmol Vis Sci 48(5) Pages: 825-832
  • Peer Reviewed
• [Journal Article] Retinal circulatory changes associated with interferon-induced retinopathy in patients with hepatitis C (2007)
  • Author(s): Nagaoka T, Sato E, Takahashi A, Yokohama S, Yoshida A
  • Journal Title: Invest Ophthalmol Vis Sci 48(1) Pages: 368-375
  • Peer Reviewed
• [Journal Article] Retinal circulatory changes and interferon therapy in patients with hepatitis C : role of retinal circulation in interferon-induced retinopathy. (2007)
  • Author(s): Nagaoka T, Sato E, Takahashi A, Yokohama S, Yoshida A.
  • Journal Title: Investigative Ophthalmollogy & Visual Science 48 Pages: 368-375
  • Peer Reviewed
• [Journal Article] Simvastatin elicits dilation of isolated porcine retinal arterioles : role of nitric oxide and mevalonate-rho kinase pathway (2007)
  • Author(s): Nagaoka T, Hein T, Yoshida A, Kuo L
  • Journal Title: Investigative Ophthalmollogy & Visual Science 48 Pages: 825-832
  • Peer Reviewed
• [Journal Article] Prorenin levels in retinopathy of prematurity. (2007)
  • Author(s): Yokota H, Nagaoka T, Mori F,, et al
  • Journal Title: American Journal of Ophthalmology 143 Pages: 531-533
  • Peer Reviewed
• [Journal Article] Resveratrol, a constituent of red wine, elicits dilation of isolated porcine retinal arterioles : role of nitric oxide and potassium channels. (2007)
  • Author(s): Nagaoka T, Hein T, Yoshida A, Kuo L
  • Journal Title: Investigative Ophthalmollogy & Visual Science 48 Pages: 4232-4239
  • Peer Reviewed
• [Journal Article] Functional and Molecular Characterization of the Endothelin System in Retinal Arterioles
  • Author(s): Hein TW, Ren Y, Yuan Z, Xu W, Somvanshi S, Nagaoka T, Yoshida A, Kuo L
  • Journal Title: Invest Ophthalmol Vis Sci (in press)
  • Peer Reviewed
• [Journal Article] Macular Microcirculation and Macular Oedema in Branch Retinal Vein Occlusion
  • Author(s): Noma H, Funatsu H, Sakata K, Harino S, Nagaoka T, Mimura T, Sone T, Hori S.
  • Journal Title: Br J Ophthalmol (in press)
  • Peer Reviewed
• [Journal Article] Effect of Systemic Administration of Valsaltan, an Angiotensin II Type 1 Receptor Blocker, on Retinal Circulation in healthy humans
  • Author(s): Nagaoka T, Takahashi A, Sato E, Yokota A, Izumi N, Takahashi A, Yoshida A
  • Journal Title: Eye (in press)
  • Peer Reviewed
• [Presentation] Change in Retinal Blood Flow Associated With Duration of Type 2 Diabetes Mellitus (2008)
  • Author(s): Nagaoka T
  • Organizer: ARVO meeting
  • Place of Presentation: Fort Lauderdale, FL, USA
  • Year and Date: 2008-05-02
• [Presentation] Effect of Panretinal Photocoagulation on Pulsatile Ocular Blood Flow in Patients With Proliferative Diabetic Retinopathy Invest Ophthalmol Vis Sci 2008; E-Abstract 2740 (2008)
  • Author(s): A. Takahashi, T. Nagaoka, E. Sato, A. Yoshida
  • Organizer: Annual meeting of the association of research in vision and Ophthalmology
  • Place of Presentation: 米国、フロリダ
• [Presentation] 1.Reduced Compliance of the Retinal Arteriolar Circulation With Increased Serum Prorenin in Patients With Type 2 Diabetes Invest Ophthalmol Vis Sci 2008; E-Abstract 2741 (2008)
  • Author(s): H. Yokota, T. Nagaoka, A. Takahashi1, E. Sato, A. Yoshida
  • Organizer: Annual meeting of the association of research in vision and Ophthalmology
  • Place of Presentation: 米国、フロリダ
• [Presentation] Change in Retinal Blood Flow Associated With Duration of Type 2 Diabetes Mellitus Invest Ophthalmol Vis Sci 2008; E-Abstract 3509 (2008)
  • Author(s): T. Nagaoka, A. Takahashi, H. Yokota, E. Sato, K. Sogawa, A. Yoshida
  • Organizer: Annual meeting of the association of research in vision and Ophthalmology
  • Place of Presentation: 米国、フロリダ
• [Presentation] Acute Hyperglycemia Induces the Endothelial Dysfunction of Retinal Arteries Invest Ophthalmol Vis Sci 2008; E-Abstract 5364 (2008)
  • Author(s): K. Sogawa, T. Nagaoka, E. Sato, A. Takahashi, N. Izumi, A. Yoshida
  • Organizer: Annual meeting of the association of research in vision and Ophthalmology
  • Place of Presentation: 米国、フロリダ
• [Presentation] C-reactive Protein Inhibits Endothelium-Dependent Nitric Oxide-Mediated Dilation of Isolated Porcine Retinal Arterioles. (2007)
  • Author(s): Nagaoka T
  • Organizer: ARVO meeting
  • Place of Presentation: Fort Lauderdale, FL, USA
  • Year and Date: 2007-04-24
• [Presentation] Role of Nitric Oxide in Regulation of Retinal Blood Flow in Response to Hyperoxia in Cats Invest Ophthalmol Vis Sci 2007; E-Abstract 871 (2007)
  • Author(s): N. Izumi, T. Nagaoka, E. Sato, K. Sogawa, H. Kagokawa, A. Takahashi, A. Kawahara, A. Yoshida
  • Organizer: Annual meeting of the association of research in vision and Ophthalmology
  • Place of Presentation: 米国、フロリダ
• [Presentation] Relation Between Serum Levels of Prorenin and Retinal Circulation in Patients With Type 2 Diabetes Mellitus Without Retinopathy Invest Ophthalmol Vis Sci 2007; E-Abstract 3765 (2007)
  • Author(s): H.Yokota, T.Nagaoka, A.Takahashi, E.Sato, A.Yoshida
  • Organizer: Annual meeting of the association of research in vision and Ophthalmology
  • Place of Presentation: 米国、フロリダ
• [Presentation] Effect of Topical Tafluprost on Retinal and Ocular Nerve Head Circulation in Normal Human Eyes. Invest Ophthalmol Vis Sci 2007; E-Abstract 2280 (2007)
  • Author(s): A.Takahashi, E.Sato, T.Nagaoka, A.Yoshida
  • Organizer: Annual meeting of the association of research in vision and Ophthalmology
  • Place of Presentation: 米国、フロリダ
• [Presentation] C-reactive Protein Inhibits Endothelium-Dependent Nitric Oxide-Mediated Dilation of Isolated Porcine Retinal Arterioles. Invest Ophthalmol Vis Sci 2007; E-Abstract 2271 (2007)
  • Author(s): T.Nagaoka1,2, L.Kuo1, A.Yoshida2, T.W. Hein1
  • Organizer: Annual meeting of the association of research in vision and Ophthalmology
  • Place of Presentation: 米国、フロリダ
• [Presentation] Retinal Hemodynamics in Patients With Normal Tension Glaucoma Invest Ophthalmol Vis Sci 2007; E-Abstract 4396 (2007)
  • Author(s): E.Sato, A.Takahashi, M.Kawai, T.Nagaoka, A.Yoshida
  • Organizer: Annual meeting of the association of research in vision and Ophthalmology
  • Place of Presentation: 米国、フロリダ
• [Presentation] Role of VEGFR2 in the Dilation of Retinal Arterioles to Increased Lumenal Flow. Invest Ophthalmol Vis Sci 2007; E-Abstract 6041 (2007)
  • Author(s): R.H. Rosa, Jr., T.W. Hein, T.Nagaoka, W.Xu, Z.Yuan, L.Kuo
  • Organizer: nnual meeting of the association of research in vision and Ophthalmology
  • Place of Presentation: 米国、フロリダ
• [Book] 眼科臨床に必要な解剖生理, VI. 網膜・脈絡膜 4.網膜の血流. 眼科プラクティス6 (2007)
  • Author(s): 長岡泰司
  • Publisher: 文光堂
• [Remarks]
  • URL: http://www.asahikawamed.ac.jp/dept/mc/ophtha/
• [Remarks]
  • URL: http://www.asahikawa-med.ac.jp/dept/mc/ophtha/