Functional Tooth Root Regeneration with Dental Pulp Stem Cells and Periodontal Ligament Stem Cells

• Project/Area Number: 19689038
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: 補綴理工系歯学
• Research Institution: Okayama University
• Principal Investigator: SONOYAMA Wataru Okayama University, 岡山大学病院, 助教 (40325121)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥26,650,000 (Direct Cost: ¥20,500,000、Indirect Cost: ¥6,150,000); Fiscal Year 2009: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000); Fiscal Year 2008: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000); Fiscal Year 2007: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
• Keywords: 組織再生 / 組織幹細胞 / 歯根 / 歯

Research Abstract

Dental pulp stem cells and periodontal ligament stem cells were successfully isolated from extracted teeth of experimental beagle dogs. These cells were confirmed to regenerate mineralized tissue when transplanted into immunocompromised mice. It was shown that surrounding mesenchymal cells regulates maturation of human ameloblastic cells. It was proved that simvastatin is possibly used as pulp capping agent that enhances reparative dentinogenesis.

Research Products

• [Journal Article] Simvastatin induces the odontogenic differentiation of human dental pulp stem cells (DPSCs) in vitro and in vivo. (2009)
  • Author(s): Y. Okamoto, W. Sonoyama, M. Ono, K. Akiyama, T. Fujisawa, M. Oshima, Y. Tsuchimoto, Y. Matsuka, T. Yasuda, S. Shi, T. Kuboki.
  • Journal Title: Journal of Endodontics 35(3) Pages: 367-372
  • Peer Reviewed
• [Journal Article] Simvastatin induces the odontogenic differentiation of human dental pulpstem cells (DPSCs) in vitro and in vivo (2009)
  • Author(s): Y. Okamoto, W. Sonoyama, 他9名
  • Journal Title: Journal of Endodontics 35 Pages: 367-372
  • Peer Reviewed
• [Journal Article] bFGF局所投与による下顎骨骨質改善効果の検討-神経提由来骨髄細胞と中胚葉由来骨髄細胞の骨芽細胞分化ならびに破骨細胞分化の相違とbFGFへの反応性- (2009)
  • Author(s): 大島正充
  • Journal Title: 岡山歯学会雑誌 28(印刷中)
  • Peer Reviewed
• [Journal Article] The Hidden Treasure in Apical Papilla: Potential Role in Pulp/Dentin Regeneration and BioRoot Engineering. (2008)
  • Author(s): G.T.J. Huang, W. Sonoyama, Y. Liu, L. He, S. Wang, S. Shi.
  • Journal Title: Journal of Endodontics 34(6) Pages: 645-651
  • Peer Reviewed
• [Journal Article] Characterization of Apical Papilla and its Residing Stem Cells from Human Immature Permanent Teeth - A Pilot Study. (2008)
  • Author(s): W. Sonoyama, Y. Liu, T. Yamaza, R.S. Tuan, S. Wang, S. Shi, G.T.J. Huang
  • Journal Title: Journal of Endodontics 34(2) Pages: 166-171
  • Peer Reviewed
• [Presentation] 組織再生による口腔機能の再生. (2009)
  • Author(s): 園山亘
  • Organizer: 岡山大学大学院医歯薬学総合研究科機能再生・再建科学専攻主催ミニ・シンポジウム
  • Place of Presentation: 岡山
  • Year and Date: 2009-08-11
• [Presentation] 組織幹細胞の制御による組織再生 -歯の機能の回復と維持に向けて (2009)
  • Author(s): 園山亘
  • Organizer: 岡山大学大学院医歯薬学総合研究科機能再生・再建科学専攻主催ミニ・シンポジウム
  • Place of Presentation: 岡山
  • Year and Date: 2009-08-11
• [Presentation] Simvastatin enhances differentiation of human dental pulp stem cells (2009)
  • Author(s): Y. Okamoto, W. Sonoyama, 他7名
  • Organizer: 6th ISSCR Annual Meeting
  • Place of Presentation: Philadelphia, PA, USA
  • Year and Date: 2009-06-12
• [Presentation] シンバスタチンによるヒト歯髄幹細胞の増殖制御と硬組織形成促進. (2009)
  • Author(s): 岡本洋介, 園山亘, 大野充昭, 秋山謙太郎, 藤澤拓生, 大島正充, 土本洋平, 松香芳三, 窪木拓男
  • Organizer: 社団法人日本補綴歯科学会第118回学術大会
  • Place of Presentation: 京都
  • Year and Date: 2009-06-06
• [Presentation] シンバスタチンによるヒト歯髄幹細胞の増殖制御と硬組織形成促進 (2009)
  • Author(s): 岡本洋介, 園山亘, ら
  • Organizer: 社団法人日本補綴歯科学会 第118回学術大会
  • Place of Presentation: 京都
  • Year and Date: 2009-06-06
• [Presentation] Characterization of putative amelogenic cells isolated from human dental follicle. (2009)
  • Author(s): Y. Tsuchimoto, W. Sonoyama, S. Shinkawa, Y. Okamoto, M. Oshima, M. Ueda, Y. Oida, Y. Matsuka, T. Kuboki
  • Organizer: 6th Biennial Congress of Asian Academy of Prosthodontics.
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2009-04-25
• [Presentation] Characterization of putative amelogenic cells isolated from human dental follicle (2009)
  • Author(s): Y.Tsuchimoto, W.Sonoyama, et al
  • Organizer: 6th Biennial Congress of Asian Academy of Prosthodontic
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2009-04-25
• [Presentation] 組織幹細胞の制御による組織再生-歯の機能の回復と維持に向けて- (2009)
  • Author(s): 園山亘, 窪木拓男
  • Organizer: 歯と顔の発生・再生シンポジウム
  • Place of Presentation: 岡山
  • Year and Date: 2009-03-27
• [Presentation] Simvastatin Enhances Differentiation of Human Dental Pulp Stem Cells. (2008)
  • Author(s): Y. Okamoto, W. Sonoyama, M. Ono, K. Akiyama, T. Fujisawa, M. Oshima, Y. Tsuchimoto, Y. Matsuka, T. Yasuda, S. Shi, T. Kuboki.
  • Organizer: 第56回JADR総会・学術大会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-11-29
• [Presentation] Simvastatin Enhances Differentiation of Human Dental Pulp Stem Cells (2008)
  • Author(s): Y. Okamoto, W. Sonoyama, 他9名
  • Organizer: 第56回JADR総会・学術大会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-11-29
• [Presentation] Mesenchymal Stem Cell- Mediated Functional Tooth Regeneration in Swine. (2008)
  • Author(s): 園山亘
  • Organizer: 第29回岡山歯学会総会・学術大会
  • Place of Presentation: 岡山
  • Year and Date: 2008-11-24
• [Presentation] bFGF局所投与における顎骨骨質改善-ウサギモデルにおける組織学的・細胞生物学的検討- (2008)
  • Author(s): 大島正充, 園山亘, 他6名
  • Organizer: 第26回日本骨代謝学会
  • Place of Presentation: 大阪
  • Year and Date: 2008-10-29
• [Presentation] Improvement of Alveolar Bone Quality by Local bFGF Injection-Histological and Cellular Biological Analysis in a Rabbit Model- (2008)
  • Author(s): M. Oshima, W. Sonoyama, 他7名
  • Organizer: ASBMR 30th Annual Meeting
  • Place of Presentation: Montreal, Quebec, Canada
  • Year and Date: 2008-09-13
• [Presentation] 組織幹細胞による組織再生再生歯科医療はQOL向上に貢献できるか?口腔からQOL向上を目指す連携研究 (2008)
  • Author(s): 園山亘, 窪木拓男
  • Organizer: 再生工学カテゴリー第一回研究集会
  • Place of Presentation: 広島
  • Year and Date: 2008-09-10
• [Presentation] Improvement of Alveolar Bone Quality by Local bFGF Injection-Histological and Cellular Biological Analysis in a Rabbit Model- (2008)
  • Author(s): M. Oshima, W. Sonoyama, 他7名
  • Organizer: 4th Scientific Meeting of the Asian Academy of Osseointegration
  • Place of Presentation: Hiroshima, JAPAN
  • Year and Date: 2008-07-19
• [Presentation] bFGF局所投与における顎骨骨質改善-ウサギモデルにおける組織学的・細胞生物学的検討- (2008)
  • Author(s): 大島正充,園山亘, 他7名
  • Organizer: 第29回日本炎症再生医学会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-09
• [Presentation] Characterization of putative amelogenic cells isolated from human dental follicle. (2008)
  • Author(s): Y. Tsuchimoto, W. Sonoyama, Y. Okamoto, M. Oshima, Y. Matsuka, T. Kuboki.
  • Organizer: 86th General Session & Exhibition of the IADR.
  • Place of Presentation: Toronto, Ontario, Canada
  • Year and Date: 2008-07-03
• [Presentation] Characterization of putative amelogenic cells isolated from human dental follicl (2008)
  • Author(s): Y. Tsuchimoto, W, Sonoyama, 他4名
  • Organizer: 86th General Session & Exhibition of the IADR
  • Place of Presentation: Toronto, Ontario, Canada
  • Year and Date: 2008-07-03
• [Presentation] Simvastatin enhances differentiation of human dental pulp stem cells. (2008)
  • Author(s): Y. Okamoto, M. Ono, W. Sonoyama, T. Fujisawa, M. Oshima, Y. Tsuchimoto, T. Yasuda, Y. Matsuka, T. Kuboki
  • Organizer: 6th ISSCR Annual Meeting.
  • Place of Presentation: Philadelphia, PA, USA
  • Year and Date: 2008-06-12
• [Presentation] HMG-CoA還元酵素阻害薬スタチンによるヒト歯髄幹細胞の増殖と分化の制御 (2008)
  • Author(s): 岡本洋介, 大野充昭, 藤澤拓生, 大島正充, 土本洋平, 松香芳三, 保田立二, 園山亘, 窪木拓男
  • Organizer: 第7回日本再生医療学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-03-14
• [Presentation] 口腔組織の再生医療へ向けた複合的アプローチ. (2008)
  • Author(s): 園山亘
  • Organizer: 第5回産学連携フォーラム(歯科再生医療産学連携会議)
  • Place of Presentation: 名古屋
  • Year and Date: 2008-03-14
• [Presentation] ヒト抜去歯からの新規の組織幹細胞の同定とその応用による機能的歯根再生. (2008)
  • Author(s): 園山亘
  • Organizer: 第7回日本再生医療学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-03-14
• [Presentation] HMG-CoA 還元酵素阻害薬スタチンによるヒト歯髄幹細胞の増殖と分化の制御 (2008)
  • Author(s): 岡本洋介, 園山 亘, 他7名
  • Organizer: 第7回日本再生医療学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-03-14
• [Presentation] ヒト抜去歯からの新規の組織幹細胞の同定とその応用による機能的歯根再生 (2008)
  • Author(s): 園山 亘
  • Organizer: 第7回日本再生医療学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-03-14
• [Presentation] 口腔組織の再生医療へ向けた複合的アプローチ (2008)
  • Author(s): 園山 亘
  • Organizer: 第5回産学連携フォーラム(歯科再生医療産学連携会議)
  • Place of Presentation: 名古屋
  • Year and Date: 2008-03-14
• [Presentation] 根未完成歯における新規間葉系幹細胞の同定とその応用による歯根再生. (2007)
  • Author(s): 園山亘, 窪木拓男, Songtao Shi
  • Organizer: ニューフロンティア補綴学リサーチカンファレンス2007.
  • Place of Presentation: 小倉
  • Year and Date: 2007-12-16
• [Presentation] 根未完成歯における新規間葉系幹細胞の同定とその応用による歯根再生 (2007)
  • Author(s): 園山 亘, (筆頭演者), 他2名
  • Organizer: ニューフロンティア補綴学リサーチカンファレンス2007
  • Place of Presentation: 小倉
  • Year and Date: 2007-12-16
• [Presentation] Effects of IL-1b and LPS on CCN2/CTGF expression in mouse odontoblastic cells, MDPC-23. (2007)
  • Author(s): W. Sonoyama, T. Fujisawa, Y. Okamoto, J. Uehara, Y. Matsuka, M. Takigawa, T. Kuboki
  • Organizer: 第1回日本CCNファミリー研究会
  • Place of Presentation: 岡山
  • Year and Date: 2007-10-27
• [Presentation] HMG-CoA還元酵素阻害薬スタチンが歯髄幹細胞に与える影響. (2007)
  • Author(s): 大野充昭, 岡本洋介, 藤澤拓生, 園山亘, 窪木拓男
  • Organizer: 第116回日本補綴歯科学会学術大会
  • Place of Presentation: 神戸
  • Year and Date: 2007-05-18
• [Patent(Industrial Property Rights)] 象牙質形成促進剤および象牙質形成覆髄材. (2008)
  • Inventor(s): 窪木拓男, 大野充昭, 園山亘, 藤澤拓生, 下野賢吾, 大島正充, 岡本洋介
  • Filing Date: 2008-03-28