Brain-derived neurotrophic factor is involved in maturation of inhibitory cells in developing somatosensory cortex.

• Project/Area Number: 19700293
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neuroscience in general
• Research Institution: Saitama Medical University
• Principal Investigator: ITAMI Chiaki Saitama Medical University, 医学部, 講師 (90392430)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,780,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥1,700,000 (Direct Cost: ¥1,700,000)
• Keywords: 分子・細胞神経科学 / 神経可塑性 / 大脳皮質 / 体性感覚野 / スパイクタイミング可塑性 / GIBA / BDNF / 受容野マップ可塑性 / 臨界期 / GABA作動性ニューロン

Research Abstract

BDNF欠損マウスにおいて、spike timing-dependent potentiation(STDP)誘導に対するBDNFによるCa調節の効果を検討するため、まず、野生型マウス体性感覚野より作製した標本を用いて、バレル皮質第4層刺激により誘発される第2/3層の細胞からパッチ電極を用いて電流固定化に細胞内記録を行った。奮性特有の発火特性であることを確認し、STDPが誘導できるかどうかを確認し、生後1週間前後から3週間までの野生型マウスに対してSTDPの出やすさを評価した。

Research Products

• [Journal Article] Brain-Derived Neurotrophic Factor Regulates the Maturation of Layer 4 Fast-Spiking Cells after the Second Postnatal Week in the Developing Barrel Cortex. (2007)
  • Author(s): Chiaki Itami, Fumitaka Kimura, Shun Nakamura
  • Journal Title: J. Neurosci. 27 Pages: 2241-2252
• [Presentation] Developmental changes in the fine temporal control of thalamic activation of cortical cells and associated changes in plasticity (2008)
  • Author(s): 木村文隆
  • Organizer: Ancillary and Satellite Events : Symposium ; Barrels XXI
  • Place of Presentation: Baltimore, USA
  • Year and Date: 2008-11-13
• [Presentation] フェンサイクリジンによる培養大脳皮質ニューロンの細胞内BDNF発現上昇は、その後のシナプス機能阻害の一因となっている(P1-s15) (2008)
  • Author(s): 安達直樹
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-09
• [Presentation] Phencyclidineによる大脳皮質ニューロンのシナプスの減少はBDNF放出阻害によって引き起こされる (2008)
  • Author(s): 安達直樹、沼川忠広、熊丸絵美、伊丹千晶、仙波りつ子、功刀浩
  • Organizer: 神経組織の成長・再生・移植研究会第23回学術集会
  • Year and Date: 2008-05-17
• [Presentation] Developmental changes in the fine temporal control of thalamic activation of cortical cells and associated changes in plasticity (2008)
  • Author(s): Kimura, F., Itami, C., Ikezoe, K., Tamura, H., Fujita, I., Yanagawa, Y., Obata, K., Ohshima, M.
  • Organizer: Ancillary and Satellite Events: SymposiumBarrels XXI
  • Place of Presentation: Baltimore, MD, USA
• [Presentation] フェンサイクリジンによる培養大脳皮質ニューロンの細胞内bd nf発現上昇は、その後のシナプス機能阻害の一因となっている(P1-s15) (2008)
  • Author(s): 安達直樹、沼川忠広、熊丸絵美、伊丹千晶、仙波りつ子、功刀浩
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京国際フォーラム
• [Presentation] Gene expression analysis of inhibitory cells derived from deveoping barrel cortex in BDNF knockout mice (2007)
  • Author(s): Chiaki Itami
  • Organizer: Neuroscience 2007 SfN 37th Annual Meeting
  • Place of Presentation: San Diego, California
  • Year and Date: 2007-11-06
• [Presentation] Prior activation of GABAergic neurons to excitatory neurons by thalamic axons within a single barrel in the mouse thalamocortical system: relevance to the spike timing dependent plasticity (2007)
  • Author(s): Fumitaka Kimura
  • Organizer: Neuroscience 2007 SfN 37th Annual Meeting
  • Place of Presentation: San Diego, California
  • Year and Date: 2007-11-06
• [Presentation] バレル皮質内GABA細胞への視床投射の特異性と可塑性との関与 (2007)
  • Author(s): 木村文隆、伊丹千晶、池添貢司、田村弘、藤田一郎、小幡邦彦、柳川右千夫、大島稔
  • Organizer: Neuro 2007第30回日本神経科学会大会、第50回日本神経化学会大会、第17回日本神経回路学会大会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-09-11
• [Presentation] バレル皮質内GABA細胞への視床投射の特異性と可塑性との関与Maturation of thalamocortical transmission to GABAergic neurons and its possible relevance to plasticity in the barrel cortex (2007)
  • Author(s): Fumitaka Kimura
  • Organizer: Neuro 2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-09-11
• [Presentation] Thalamic activation of GABAergic neurons leads to that of excitatory neurons within a single barrel in the mouse thalamocotical system (2007)
  • Author(s): Chiaki Itami
  • Organizer: IBRO World congress of Neuroscience
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 2007-07-13
• [Presentation] Thalamic activation of GABAergic neurons leads to that of excitatory neurons within a single barrel in the mouse thalamocortical system (2007)
  • Author(s): Fumitaka Kimura
  • Organizer: IBRO World congress of Nueroscience
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 2007-07-13
• [Presentation] Gene expression analysis of inhibitory cells derived from developing barrel cortex in BDNF knockout mice. (2007)
  • Author(s): C. Itami, H. Kumanogoh, S. Masuda, S. Takeda, K. Obata, Y. Yanagawa, S- i. Watanabe, S. Nakamura
  • Organizer: Neuroscience 2007 SfN 37thAnnual Meeting
  • Place of Presentation: San Diego, California
• [Presentation] Prior activation of GABAergic neurons to excitatory neurons by thalamic axons within a single barrel in the mouse thalamocortical system: relevance to the spike timing dependent plasticity (2007)
  • Author(s): F. Kimura, C. Itami, K. Ikezoe, H. Tamura, I. Fujita, K. Obata, Y. Yanagawa, M. Ohshima.
  • Organizer: Neuroscience 2007 SfN 37thAnnual Meeting
  • Place of Presentation: San Diego, California
• [Presentation] Thalamic activation of GABAergic neurons leads to that of excitatory neurons within a single barrel in the mouse thalamocortical system (2007)
  • Author(s): F. Kimura, C. Itami, K. Obata, Y. Yanagawa, M. Ohshima
  • Organizer: IBRO World congress of Neuroscience
  • Place of Presentation: Melbourne, Australia
• [Presentation] BDNF regulates the maturation of layer 4 fast spiking cells after the 2nd postnatal week in the developing barrel cortex (2007)
  • Author(s): C Itami, F Kimura, S Nakamura
  • Organizer: IBRO World congress of Neuroscience
  • Place of Presentation: Melbourne, Australia