Ca2+ measurement and computational analysis of population of cerebellar neurons with a two-photon microscopy

• Project/Area Number: 19700298
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neuroscience in general
• Research Institution: Osaka Bioscience Institute
• Principal Investigator: DOI Tomokazu Osaka Bioscience Institute, システムズ生物学部門, 研究員 (20435564)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,340,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥540,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥1,000,000 (Direct Cost: ¥1,000,000)
• Keywords: 神経情報処理 / 二光子顕微鏡 / 数理的解析 / カルシウム / イメージング / 小脳

Research Abstract

I performed Ca2+ fluorescence imaging in the cerebellar cortex with a two-photon and confocal microscopes. Ca2+ fluorescence intensity transiently increased by the electrical stimulation of the ipsilateral hindlimb. Spontaneous activity synchronized in neighbor Purkinje neurons. The synchronization also occurred in stellate cells, interneurons in the cerebellar cortex and stronger correlation was observed to the direction of the anterior-posterior axis. A boundary of the synchronization was not found in the field-of-view.

Research Products

• [Journal Article] A kinetic model of dopamine- and calcium-dependent striatal synaptic plasticity (2010)
  • Author(s): Nakano T, Doi T, Yoshimoto J, Doya K.
  • Journal Title: PLoS Comput Biol vol.6(2)
  • Peer Reviewed
• [Journal Article] Systems biology perspectives on cerebellar long-term depressio (2008)
  • Author(s): Ogasawara H, Doi T, Kawato M
  • Journal Title: NeuroSignals vol.16 Pages: 300-317
• [Journal Article] Ca2+ requirements for cerebellar long-term synaptic depression: role for a postsynaptic leaky integrator (2007)
  • Author(s): Tanaka K, Khiroug L, Santamaria F, Doi T, Ogasawara H, Ellis-Davies GC, Kawato M
  • Journal Title: Augustine GJ. Neuron vol.54(5) Pages: 787-800
  • Peer Reviewed
• [Journal Article] Nitric oxide regulates input specificity of long-term depression and context dependence of cerebellar learning (2007)
  • Author(s): Ogasawara H, Doi T, Doya K, Kawato M
  • Journal Title: PLoS Computational Biology vol.3
  • Peer Reviewed
• [Presentation] ImageJとMATLABを使った蛍光変化率の計算 (2009)
  • Author(s): 土居智和
  • Organizer: 定量生物学の会 第1回年会
  • Place of Presentation: 東京大学 生産技術研究所
  • Year and Date: 2009-01-10
• [Presentation] ImageJとMATLABを使った蛍光変化率の計算 (2009)
  • Author(s): 土居智和
  • Organizer: 定量生物学の会第1回年会
  • Place of Presentation: 東京大学生産技術研究所
  • Year and Date: 2009-01-10
• [Presentation] チュートリアル画像解析入門「ImageJとMATLABを使った蛍光変化率の計算」 (2009)
  • Author(s): 土居智和
  • Organizer: 定量生物学の会第一回年会東京大学生産技術研究所(駒場)An棟コンベンションホール・会議
  • Place of Presentation: 大阪バイオサイエンス研究所・所長研究部
• [Presentation] 小脳シナプス可塑性におけるシグナル伝達の計算機シミュレーション (2007)
  • Author(s): 土居 智和
  • Organizer: シナプス研究会
  • Place of Presentation: 生理学研究所
  • Year and Date: 2007-12-06
• [Presentation] 小脳シナプス可塑性におけるシグナル伝達の計算機シミュレーション (2007)
  • Author(s): 土居智和
  • Organizer: 2007年度シナプス研究会『シナプスの形成と成熟の分子機序』自然科学研究機構
  • Place of Presentation: 生理学研究所(愛知県岡崎市)1階会議室