Ca2+ measurement and computational analysis of population of cerebellar neurons with a two-photon microscopy
| Project/Area Number |
19700298
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neuroscience in general
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| Research Institution | Osaka Bioscience Institute |
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Principal Investigator |
DOI Tomokazu Osaka Bioscience Institute, システムズ生物学部門, 研究員 (20435564)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥3,340,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | 神経情報処理 / 二光子顕微鏡 / 数理的解析 / カルシウム / イメージング / 小脳 |
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| Research Abstract |
I performed Ca2+ fluorescence imaging in the cerebellar cortex with a two-photon and confocal microscopes. Ca2+ fluorescence intensity transiently increased by the electrical stimulation of the ipsilateral hindlimb. Spontaneous activity synchronized in neighbor Purkinje neurons. The synchronization also occurred in stellate cells, interneurons in the cerebellar cortex and stronger correlation was observed to the direction of the anterior-posterior axis. A boundary of the synchronization was not found in the field-of-view.
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Report
(4 results)
Research Products
(9 results)
