| Project/Area Number |
19700313
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neuroscience in general
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
SUGA Akiko The Institute of Physical and Chemical Research, 網膜再生医療研究チーム, 研究員 (70450400)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥3,350,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥450,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | 網膜 / 神経発生 / ニワトリ胚 / サブタイプ / 樹状突起形態 / 転写因子 / 障害 / 再生 / ミュラーグリア細胞 / 発生 / 神経 / 時期特異的遺伝子導入 / ニワトリ / 細胞形態 / LIMホメオドメイン型転写因子 |
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| Research Abstract |
In nervous system, transcription factor expression in progenitor cells regulates cell type specification. However, whether or not these transcription factors regulate later developmental events remains an open question. We found that two LIM-homeodomain transcription factors, Lim1 and Islet1, are expressed in distinct subtype of retinal horizontal cells (Lim1 : type I, Islet1 : type II/III). Overexpression of Islet1 in post-migratory horizontal cells repressed endogenous Lim1 expression and increased the cells with type II cell specific morphology. Inhibition of Lim1 function perturbed axonal morphogenesis of type I horizontal cells. Our results suggest that LIM-homeodomain transcription factors are required for subtype-specific morphogenesis of horizontal cells at later stages of retinal development.
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