Effect of PACAP on reactive astrocyte in vivo and in vitro

• Project/Area Number: 19700322
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: Showa University
• Principal Investigator: NAKAMACHI Tomoya Showa University, 医学部, ポストドクター (30433840)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,560,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2007: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: 細胞・組織 / 脳神経疾患 / 病理学 / 神経細胞病理学 / 神経組織細胞化学 / 神経病態薬理学 / 脳血管障害学 / アストロサイト

Research Abstract

虚血性神経細胞死誘導後に認められるグリオーシス(アストロサイトの活性)におけるPACAPの作用を検討した。PACAP受容体は神経損傷領域を取り囲むアストロサイトにおいて強く発現し、培養細胞を用いた実験により、PACAPはアストロサイトの増殖を促進する作用を有することが明らかになった。本研究により、PACAPがアストロサイト活性化およびグリオーシス形成機構に重要な役割を担うと考えられる。

Research Products

• [Journal Article] Endogenous pituitary adenylate cyclase activating polypeptide (PACAP) is involved in suppression of edema in the ischemic brain (2009)
  • Author(s): Nakamachi T, Ohtaki H, Yofu S, Dohi K, Watanabe J, Mori H, Sato A, Hashimoto H, Shintani N, Baba A, Shioda S.
  • Journal Title: Acta Neurochir Suppl (in press)
• [Journal Article] Isolation of peptide transport system-6 from brain endothelial cells: therapeutic effects with antisense inhibition in Alzheimer and stroke models (2009)
  • Author(s): Dogrukol-Ak D, Kumar VB, Ryerse JS, Farr SA, Verma S, Nonaka N, Nakamachi T, Ohtaki H, Niehoff ML, Edwards JC, Shioda S, Morley JE, Banks WA
  • Journal Title: J Cereb Blood Flow Metab 19 Pages: 411-422
• [Journal Article] Endogenous pituitary adenylate cyclase activating polypeptide (PACAP) is involved in suppression of edema in the ischemic brain (2009)
  • Author(s): Nakamachi T
  • Journal Title: Acta Neurochir Suppl (in press)
  • Peer Reviewed
• [Journal Article] Suppression of Ganglion Cell Death by PACAP Following Optic Nerve Transection in the Rat (2008)
  • Author(s): Seki T, Itoh H, Nakamachi T, Shioda S
  • Journal Title: J Mol Neurosci 36 Pages: 57-60
• [Journal Article] Role of PACAP in ischemic neural death. J (2008)
  • Author(s): Ohtaki H, Nakamachi T, Dohi K, Shioda S
  • Journal Title: Mol Neurosci 36 Pages: 16-25
• [Journal Article] Pituitary adenylate cyclase-activating polypeptide (PACAP) type 1 receptor (PAC1R) co-localizes with activity-dependent neuroprotective protein (ADNP) in the mouse brains (2008)
  • Author(s): Nakamachi T, Ohtaki H, Yofu S, Dohi K, Watanabe J, Hayashi D, Matsuno R, Nonaka N, Itabashi K, Shioda S
  • Journal Title: Regul Pept 145 Pages: 88-95
• [Journal Article] Distribution and localization of pituitary adenylate cyclase-activating polypeptide-specific receptor (PAC1R) in the rostral migratory stream of the infant mouse brain (2008)
  • Author(s): Matsuno R, Ohtaki H, Nakamachi T, Watanabe J, Yofu S, Hayashi D, Takeda T, Nonaka N, Seki M, Nakamura M, Itabashi K, Shioda S
  • Journal Title: Regul Pept 145 Pages: 80-87
• [Journal Article] Pituitary adenylate cyclase-activating polypeptide (PACAP) type I receptor (PACII) co-localizes with activity-dependent neuroprotective protein (ADNP) in the mouse brains (2008)
  • Author(s): Nakamachi T
  • Journal Title: Regul Pert 145(1-3) Pages: 88-95
  • Peer Reviewed
• [Journal Article] Suppression of Ganglion Cell Death by PACAP Following Optic Nerve Transection in the Rat (2008)
  • Author(s): Seki T
  • Journal Title: J Mol Neurosci 36(1-3) Pages: 57-60
  • Peer Reviewed
• [Journal Article] Pituitary adenylate cyclase-activatin polypeptide (PACAP) type 1 receptor (PAC1R) co-localizes with activity-dependent neuroprotective protein (ADNP) in the mouse brains. (2008)
  • Author(s): Nakamachi, T・Ohtaki, H・Yofu, S・Dohi, K・Watanabe, J・Hayashi, D・Matsuno, R・Nonaka, N・Itabashi, K・Shioda, S.
  • Journal Title: Regul Pept 145(1-3) Pages: 88-95
  • Peer Reviewed
• [Journal Article] Distribution and localization of pituitary adenylate cyclase-activating polypeptide-specific receptor (PACIR) in the rostral migratory stream of the infant mouse brain. (2008)
  • Author(s): Matsuno, R・Ohtaki, H・Nakamachi, T・Watanabe, J・Yofu, S・Hayashi, D・Takeda, T・Nonaka, N・Seki, M・Nakamura, M・Itabashi, K・Shioda, S.
  • Journal Title: Regul Pept 145(1-3) Pages: 80-80
  • Peer Reviewed
• [Journal Article] Localization, characterization and function of pituitary adenylate cyclase-activating polypeptide during brain development.Peptides (2007)
  • Author(s): Watanabe J, Nakamachi T, Matsuno R, Hayashi D, Nakamura M, Kikuyama S, Nakajo S, Shioda S.
  • Journal Title: Review 28(9) Pages: 1713-9
• [Journal Article] Does Edaravone (MCI-186) Act as an Antioxidant and a Neuroprotector in Experimental Traumatic Brain Injury? (2007)
  • Author(s): Dohi K, Satoh K, Nakamachi T, Yofu S, Hiratsuka K, Nakamura S, Ohtaki H, Yoshikawa T, Shioda S, Aruga T
  • Journal Title: Antioxid Redox Signal 9 Pages: 281-287
• [Journal Article] Localization, characterization and function of pituitary adenylate cyclase-activating polypeptide during brain development. (2007)
  • Author(s): Watanabe, J・Nakamachi, T・Matsuno, R・Hayashi, D・Nakamura, M・Kikuyama, S・Nakajo, S・Shioda, S.
  • Journal Title: Peptides 28(9) Pages: 1713-1719
  • Peer Reviewed
• [Presentation] 反応性アストロサイトにおけるPACAP受容体発現とその機能的意義の解析 (2009)
  • Author(s): 中町智哉、押田佳菜子、養父佐知子、大滝博和、森敬善、佐藤敦、塩田清二
  • Organizer: 第114回日本解剖学会総会・全国学術集
  • Place of Presentation: 岡山
• [Presentation] 内因性PACAPによる虚血性神経細胞死抑制作用の解析 (2009)
  • Author(s): 中町智哉、大滝博和、養父佐知子、野中直子、William A. Banks、塩田清二
  • Organizer: 第114回日本解剖学会総会・全国学術集会
  • Place of Presentation: 岡山
• [Presentation] Banks and Seiji Shioda. Endogenous PACAP protects the brain against focal cerebral ischemia in mice. (2009)
  • Author(s): Tomoya Nakamachi, Hirokazu Ohtaki, Sachiko Yofu, Naoko Nonaka, William A.
  • Organizer: 17th International Symposium on Regulatory Peptides
• [Presentation] 反応性アストロサイトにおけるPACAP受容体発現とその機能的意義の解析 (2009)
  • Author(s): 中町智哉
  • Organizer: 第114回日本解剖学会総会
  • Place of Presentation: 岡山理科大学、岡山
• [Presentation] 多機能性ペプチド「PACAP」の新規生理機能の探索 (2008)
  • Author(s): 中町智哉
  • Organizer: 生体制御学特別セミナー
  • Place of Presentation: 富山大学、富山
  • Year and Date: 2008-07-19
• [Presentation] マウス涙腺組織におけるPACAP/PAC1受容体の分布局在と機能解析. (2008)
  • Author(s): 中町智哉、大滝博和、養父佐知子、会沢洋一、渡邊潤、関保、塩田清二
  • Organizer: 第33回日本比較内分泌大会
  • Place of Presentation: 広島
• [Presentation] Pituitaly adenylate cyclase-activating polypeptide (PACAP) stimulates proliferation of reactive astrocyte invitro (2008)
  • Author(s): Nakamachi T, Oshida K, Yofu S, Mori H, Watanabe J,Ohtaki H, Dohi K, Shioda S
  • Organizer: The 38th Annual Meeting of the Society for Neuroscience
  • Place of Presentation: Washington, DC
• [Presentation] Banks and Seiji Shioda. Neuroprotective effect of PACAP and the mechanisms: therapeutic effects in Stroke models (2008)
  • Author(s): Tomoya Nakamachi, Hirokazu Ohtaki, Naoko Nonaka, William A.
  • Organizer: Showa University International Symposium for Life Sciences 5th Annual Meeting
• [Presentation] Neuroprotective effect of PACAP and the mechanisms: therapeuticeffects in Stroke models (2008)
  • Author(s): Nakamachi T, Ohtaki H, Nonaka N, William A. Banks, Shioda S
  • Organizer: Showa University International Symposium for Life Science 5th Annual Meeting New Frontiers in Neuroscience : Transmitters/modulators in Health and Disease
  • Place of Presentation: 東京(シンポジウム)
• [Presentation] 下垂体アデニル酸シクラーゼ活性化ポリペプチド(PACAP)の神経幹細胞に対する作用 (2008)
  • Author(s): 中町智哉、渡邊潤、大滝博和、養父左知子、塩田清二
  • Organizer: 第35回日本神経内分泌学会・第23回日本下垂体研究会合同学術集会
  • Place of Presentation: 東京
• [Presentation] PACAPによる成体神経幹細胞の増殖促進作用 (2008)
  • Author(s): 中町智哉、渡邊潤、養父佐知子、大滝博和、野中直子、土肥謙二、塩田清二
  • Organizer: 第5回GPCR研究会
  • Place of Presentation: 東京
• [Presentation] 涙腺におけるPACAPおよびPAC1受容体の組織分布と涙液分泌促進機構 (2008)
  • Author(s): 中町智哉、大滝博和、養父佐知子、会沢洋一、渡邊潤、関保、塩田清二
  • Organizer: 第113回日本解剖学会総会・全国学術集会
  • Place of Presentation: 大分
• [Presentation] マウス成体神経幹細胞におけるPACAPおよびPAC1R受容体免疫陽性反応の分布局在 (2008)
  • Author(s): 中町智哉、養父佐知子、大滝博和、渡邊潤、森敬善、塩田清二
  • Organizer: 第113回日本解剖学会総会・全国学術集会
  • Place of Presentation: 大分
• [Presentation] PACAPはin vivoで神経幹細胞のラジアルグリアへの分化誘導に関与する (2008)
  • Author(s): 渡邊潤、中町智哉、中村正久、中条茂夫、塩田清二
  • Organizer: 第113回日本解剖学会総会・全国学術集会
  • Place of Presentation: 大分
• [Presentation] 下垂体アデニル酸シクラーゼ活性化ポリペプチド(PACAP)の神経幹細胞に対する作用 (2008)
  • Author(s): 中町智哉
  • Organizer: 第35回日本神経内分泌学会
  • Place of Presentation: 政策研究大学院大学、東京
• [Presentation] Pituitary adenylate cyclase-activating polypeptide (PACAP) stimulates proliferation of reactive astrocyte in vitro (2008)
  • Author(s): Tomoya Nakamachi
  • Organizer: The 38th Annual Meeting of the Society for Neuroscience
  • Place of Presentation: Wahington DC, USA
• [Presentation] マウス涙腺組織におけるPACAP/PAC1受容体の分布局在と機能解析 (2008)
  • Author(s): 中町智哉
  • Organizer: 第33回日本比較内分泌学会大会
  • Place of Presentation: 広島大学、広島
• [Presentation] マウス成体神経幹細胞におけるPACAPおよびPACIR受容体免疫陽性反応の分布局在 (2008)
  • Author(s): 中町 智哉、養父 佐知子、大滝 博和、渡邊 潤、森 敬善, 塩田 清二
  • Organizer: 第113回日本解剖学会総会
  • Place of Presentation: 大分 大分大学医学部
• [Presentation] Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) Attenuates Focal Cerebral Ischemic Injury via Bcl-2 and IL-6 Signaling (2007)
  • Author(s): Tomoya Nakamachi, Hirokazu Ohtaki, Kenji Dohi, Sachiko Yofu, Jun Watanabe, Daisuke Hayashi, Hitoshi Hashimoto, Norihito Shintani, Akemichi Baba, Akira Arimura and Seiji Shioda
  • Organizer: The 37th Annual Meeting of theSociety for Neuroscience
  • Place of Presentation: San Diego CA
• [Presentation] Involvement of beta isoform of protein kinase C in the PACAP-induced differentiation of neural stem cells into astrocytes (2007)
  • Author(s): Jun Watanabe, Tomoya Nakamachi, Daisuke Hayashi, Masahisa Nakamura, Shigeo Nakajo, Seiji Shioda
  • Organizer: The 37th Annual Meeting of theSociety for Neuroscience
  • Place of Presentation: San Diego CA
• [Presentation] 成体神経幹細胞に対する下垂体アデニル酸シクラーゼ活性化ポリペプチド(PACAP)の作用 (2007)
  • Author(s): 中町智哉、松野良介、林大祐、養父佐知子、大滝博和、渡邊潤、塩田清二
  • Organizer: 第32回日本比較内分泌学会大会
  • Place of Presentation: 東京
• [Presentation] in vitro及びin vivo におけるPACAPの神経幹細胞に対するアストロサイト分化誘導作用 (2007)
  • Author(s): 渡邊潤 中町智哉 中村正久 中条茂男 塩田清二
  • Organizer: 第32回日本比較内分泌学会大会
  • Place of Presentation: 東京
• [Presentation] OF PACAP ASSOCIATED WITH IL-6 SIGNALING (2007)
  • Author(s): Tomoya Nakamachi, Hirokazu Ohtaki, Kenji Dohi, Sachiko Yofu, Hitoshi Hashimoto, Norihito Shintani, Akemichi Baba, Akira Arimura, Seiji Shioda
  • Organizer: 8th International Symposium for VIP, PACAP and Related Peptides
  • Place of Presentation: Manchester, VT.
• [Presentation] Distribution of activity-dependent neuroprotective protein (ADNP) and (PACAP) type 1 receptor (PAC1R) in the mouse brain (2007)
  • Author(s): Tomoya Nakamachi, Hirokazu Ohtaki, Sachiko Yofu, Jun Watanabe, Daisuke Hayashi and Seiji Shioda
  • Organizer: 8th International Symposium for VIP, PACAP and Related Peptides
  • Place of Presentation: Manchester, VT.
• [Presentation] THE ROLE OF PACAP IN ASTROGLISL DIFFERENTIATION IN VIVO AND IN VITRO (2007)
  • Author(s): Jun Watanabe, Tomoya Nakamachi, Daisuke Hayashi, Masahisa Nakamura, Shigeo Nakajo and Seiji Shioda
  • Organizer: 8th International Symposium for VIP, PACAP and Related Peptides
  • Place of Presentation: Manchester, VT.
• [Presentation] Effect of PACAP on neuronal progenitor cells in vivo; 8th International Symposium for VIP (2007)
  • Author(s): Daisuke Hayashi, Tomoya. Nakamachi, Ryosuke Matsuno, Jun Watanabe, Seiji Shioda
  • Organizer: 8th International Symposium for VIP, PACAP and Related Peptides
  • Place of Presentation: Manchester, VT
• [Presentation] マウス脳におけるADNPとPACAP特異的受容体の分布・局在 (2007)
  • Author(s): 中町智哉、養父佐知子、渡邊潤、林大祐、大滝博和、塩田清二
  • Organizer: 第4回GPCR研究会
  • Place of Presentation: 東京
• [Presentation] NEUROPROTECTIVE MECHANISM OF PACAP ASSOCIATED WITH IL-6 SIGNAJING (2007)
  • Author(s): Tomoya, Nakamachi・Hirokazu, Ohtaki・Kenji, Dohi・Sachiko, Yofu・Hitoshi, Hashimoto・Norihito, Shintani・Akemichi, Baba・Akira, Arimura・Seiji, Shioda
  • Organizer: 8th International Symposium for VIP, PACAP and Related Peptides
  • Place of Presentation: Manchester, Vermont USA
• [Presentation] Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) Attenuates Focal Cerebral Ischemic Injury via Bcl-2 and IL-6 Signaling (2007)
  • Author(s): Tomoya, Nakamachi・Hirokazu, Ohtaki・Kenji, Dohi・Sachiko, Yofu・Jun Watanabe・Daisuke, Hayashi・Hitoshi, Hashimoto・Norihito, Shintani・Akemichi, Baba・Akira, Arimura・Seiji, Shioda
  • Organizer: The 37th Annual Meeting of the Society for Neuroscience
  • Place of Presentation: San Diego, California USA
• [Presentation] 成体神経幹細胞に対する下垂体アデニル酸シクラーゼ活性化ポリペプチド(PACM)の作用 (2007)
  • Author(s): 中町 智哉、松野 良介、林 大祐、養父 佐知子、大滝 博和、渡邊 潤、塩田 清二
  • Organizer: 第32回日本比較内分泌学会大会
  • Place of Presentation: 栃木 日光プリンスホテル
• [Book] Increased behavioral activity with regular circadian rhythm in PACAP specific receptor (PAC1) transgenic mice (2009)
  • Author(s): Arata T, Hosono T, Taketomi Y, Kageyama H, Nakamachi T, Shioda S
  • Publisher: Transmitters and Modulators in Health and Disease -New Frontiers in Neuroscience-, Springer-Verlag, (Tokyo)(in press)
• [Book] Expression and localization of pituitary adenylate cyclase-activating polypeptide (PACAP) specific receptor (PAC1R) after traumatic brain injury in mice (2009)
  • Author(s): Morikawa K, Dohi K, Yofu S, Mihara Y, Nakamachi T, Ohtaki H, Shioda S, Aruga T
  • Publisher: Transmitters and Modulators in Health and Disease -New Frontiers in Neuroscience-, Springer-Verlag, (Tokyo),(in press)
• [Book] Increased behavioral activity with regular circadian thythm in PACAP specific receptor (PACIR) transgenic mouse, Transmitters and Modulators in Health and Disease, New Frontiers in Neuroscience (2009)
  • Author(s): Arata S
  • Publisher: Springer
• [Book] Expression and localization of pituitary adenylate cyclase-activating polypeptide (PACAP) specific receptor (PAC1R) after traumatic brain injury in mice, Transmitters and Modulators in Health and Disease, New Frontiers in Neuroscience (2009)
  • Author(s): Morikawa K
  • Publisher: Springer
• [Book] Does PACAP have therapeutic potential in the field of neuroregenerative medicine? (2007)
  • Author(s): Ohtaki H, Nakamachi T, Watanabe J, Yofu S, Matsunaga M, Matsuno R, Dohi K, Shioda S.
  • Publisher: Kusano M, Shioda S eds. New Frontiers in regenerative Medicine, Springer-Verlag
• [Remarks]
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• [Remarks]
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