Physiological function of retinoic acid-induced nNOS expression in the retina: Application for CNS regeneration

• Project/Area Number: 19700338
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Kanazawa University
• Principal Investigator: KORIYAMA Yoshiki Kanazawa University, 医学系, 助教 (70397199)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,190,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥1,500,000 (Direct Cost: ¥1,500,000)
• Keywords: 神経再生 / 一酸化窒素合成酵素 / 一酸化窒素 / cGMP / レチノイン酸 / レチノイン酸合成酵素 / 網膜 / PKG / 魚類 / アポトーシス / 視神経 / 緑内障 / RALDH

Research Abstract

ほ乳類と異なり、魚類では中枢神経の再生が可能である。これまで我々は魚類視神経損傷後の網膜内で一酸化窒素合成酵素(NOS)の発現が高まることを見つけていた。本研究ではNOSと神経再生の関連について詳細なシグナル解析とともに調べた。その結果、視神経損傷後の網膜神経節細胞層で増加する神経型のNOSはレチノイン酸で誘導され細胞内で産生された一酸化窒素がグアニル産シクラーゼ活性化を介してcGMP依存的な軸索伸長を示すことがわかった。

Research Products

• [Journal Article] Involvement of retinoic acid signaling in goldfish optic nerve regeneration (2009)
  • Author(s): Nagashima M, Sakurai H, Mawatari K, Koriyama Y, Matsukawa T, Kato S.
  • Journal Title: Neurochem Int. 54 Pages: 229-236
  • Peer Reviewed
• [Journal Article] Involvement of retinoic acid signaling in goldfish optic nerve regeneration (2009)
  • Author(s): 永島幹子
  • Journal Title: Neurochem Int. 54 Pages: 229-236
  • Peer Reviewed
• [Journal Article] A novel neuroprotective role of a small peptide from flesh fly, 5-S-GAD in the rat retina in vivo (2008)
  • Author(s): Koriyama Y, Tanii H, Ohno M, Kimura T, Kato S.
  • Journal Title: Brain Res. 1240 Pages: 196-203
  • NAID: 120001130553
  • Peer Reviewed
• [Journal Article] Changes of phospho-growth associated protein 43 (phospho-GAP43) in zebrafish retina after optic nerve injury:a long term observation (2008)
  • Author(s): Kaneda M, Nagashima M, Nunome T, Muramatsu T, Yamada Y, Kubo M, Muramoto K, Matsukawa T, Koriyama Y, Sugitani K, Vachkov I, Mawatari K, Kato S.
  • Journal Title: Neurosci Res. 61 Pages: 281-288
  • Peer Reviewed
• [Journal Article] A novel neuroprotective role of a small peptide from flesh fly, 5-S-GAD in the rat retina in vivo (2008)
  • Author(s): 郡山恵樹
  • Journal Title: Brain Res. 1240 Pages: 196-203
  • NAID: 120001130553
  • Peer Reviewed
• [Journal Article] Changes of phospho-growth assotiated protein 43 (phospho-GAP43) in zebrafish retina after optit nerve injury : a long term observation (2008)
  • Author(s): 金田学
  • Journal Title: Neurosci Res. 61 Pages: 281-288
  • Peer Reviewed
• [Journal Article] Arachidonic acid promotes glutamate-induced cell death associated with necrosis by 12-lipoxygenase activation in glioma cells (2007)
  • Author(s): Higuchi Y. Tanii H. Koriyama Y. Mizukami Y. Yoshimoto T.
  • Journal Title: Life Sciences 80 Pages: 1856-1864
  • Peer Reviewed
• [Journal Article] Early downregulation of IGF-I decides the fate of rat retinal ganglion cells after optic nerve injury (2007)
  • Author(s): Koriyama Y. Homma K. Mawatari K. Higuchi Y. Matsukawa T. Kosaka J. Kato S.
  • Journal Title: Neurochem Int 50 Pages: 741-748
  • Peer Reviewed
• [Journal Article] Upregulation of IGF-I in the goldfish retinal ganglion cells during the early stage of optic nerve regeneration (2007)
  • Author(s): Koriyama Y. Homma K. Sugitani K. Higuchi Y. Matsukawa T. Murayama D. Kato S.
  • Journal Title: Neurochem Int 50 Pages: 749-756
  • NAID: 120001450995
  • Peer Reviewed
• [Journal Article] Upregulation of IGF-I in the goldfish retinal ganglion cells during the early stage of optic nerve regeneration (2007)
  • Author(s): Koriyama Y
  • Journal Title: Neurochem Int 50 Pages: 749-756
  • NAID: 120001450995
  • Peer Reviewed
• [Journal Article] Early downregulation of IGF-I decides the fate of rat retinal ganglion cells after optic nerve injury (2007)
  • Author(s): Koriyama Y (Homma K.と共に第一著者)
  • Journal Title: Neurochem Int 50 Pages: 741-748
  • Peer Reviewed
• [Journal Article] Arachidonic acid promotes glutamate induced cell death associated with necrosis by 12-lipoxygenase activation in glioma cells (2007)
  • Author(s): Higuchi Y
  • Journal Title: Life Sciences 80 Pages: 1856-1864
  • Peer Reviewed
• [Presentation] 中枢神経損傷後におけるレチノール結合タンパク質プルプリンの神経保護、軸索伸長効果について (2009)
  • Author(s): 郡山恵樹、齊藤淳一、松川 通、加藤 聖
  • Organizer: 第129回日本薬学会年会
  • Place of Presentation: 京都
  • Year and Date: 2009-03-28
• [Presentation] A retinol binding protein, purpurin protects injury-induced apoptosis and regenerate axons in rat CNS neurons (2008)
  • Author(s): 郡山恵樹
  • Organizer: Society for Neurosciende 38th Annual Meeting
  • Place of Presentation: ワシントンDC
  • Year and Date: 2008-11-16
• [Presentation] A retinol binding protein, purpurin protects injury-induced apoptosis and regenerate axons in rat CNS neurons (2008)
  • Author(s): Y.Koriyama, J. Saito, T. Matsukawa, S.Kato.
  • Organizer: Society for Neuroscience 38th Annual Meeting
  • Place of Presentation: ワシントンDC
  • Year and Date: 2008-11-15
• [Presentation] 視神経再生分子プルプリンによるRALDH2 の発現促進作用について (2008)
  • Author(s): 齊藤淳一、郡山恵樹、野中真澄、松川通、加藤聖
  • Organizer: 第55回中部日本生理学会
  • Place of Presentation: 愛知
  • Year and Date: 2008-10-17
• [Presentation] 視神経再生分子プルプリンによるRALDH2の発現促進作用について (2008)
  • Author(s): 齊藤淳一
  • Organizer: 第55回中部日本生理学会
  • Place of Presentation: 愛知
  • Year and Date: 2008-10-17
• [Presentation] プルプリンはラット損傷中枢神経のアポトーシスを保護する (2008)
  • Author(s): 郡山恵樹、松川通、齊藤淳一、野中真澄、加藤聖
  • Organizer: 第51回日本神経化学大会
  • Place of Presentation: 富山
  • Year and Date: 2008-09-13
• [Presentation] Cell survival and axonal regeneration by purpurin in retinal ganglion cells during optic nerve injury (2007)
  • Author(s): Y. Koriyama, T. Matsukawa, H. Tanii, S. Kato
  • Organizer: Society for Neuroscience 37th Annual Meeting
  • Place of Presentation: カリフォルニア
  • Year and Date: 2007-11-06
• [Presentation] 魚類の視神経再生の評価法について (2007)
  • Author(s): 加藤 聖、永島幹子、郡山恵樹、杉谷加代、松川 通
  • Organizer: 第54回中部日本生理学会
  • Place of Presentation: 三重
  • Year and Date: 2007-10-19
• [Presentation] プルプリンによる損傷後網膜神経節細胞の生存および軸索再生 (2007)
  • Author(s): 郡山恵樹、桜井裕之、松川通、馬渡一浩、加藤聖
  • Organizer: 第50回日本神経化学大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-09-10
• [Presentation] プルプリンによる損傷後網膜神経節細胞の生存および軸索再生 (2007)
  • Author(s): 郡山 恵樹
  • Organizer: 第50回日本神経化学大会
  • Place of Presentation: 神奈川
  • Year and Date: 2007-09-10
• [Book] Optic nerve regeneration in goldfish. In: Model Organisms in Spinal Cord Regeneration.(Becker CG, Becker T, eds) (2007)
  • Author(s): Kato S. Koriyama Y. Matsukawa T. Sugitani K.
  • Publisher: Wiley-VCH, Germany
• [Book] Model Organisms in Spinal Cord Regeneration (2006)
  • Author(s): Kato S
  • Total Pages: 17
  • Publisher: Wiley-VCH, Germany
• [Remarks]
  • URL: http://web.kanazawa-u.ac.jp/%7Emed36/report.html