Processing of cell adhesion molecule L1 via neuropsin

• Project/Area Number: 19700342
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: TAMURA Hideki Nara Institute of Science and Technology, バイオサイエンス研究科, 助教授 (80437516)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥600,000); Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,300,000)
• Keywords: プロテアーゼ / プロテオリシス / プロテオーム / カリクレイン / 細胞接着分子 / 海馬 / シナプス / 神経可塑性 / セリンプロテアーゼ / 長期増強 / Gタンパク / 可塑性

Research Abstract

マウス脳において神経可塑性関連タンパク質である細胞外セリンプロテアーゼニューロプシンが直接切断するタンパク質を探索した。その結果、ニューロプシンは、神経細胞の形態変化および神経突起の伸展に関与するG protein-Regulated Inducer of Neurite outgrowth 1(GRIN1)に直接結合し、速やかに切断することを明らかとした。

Research Products

• [Journal Article] 細胞外プロテアーゼと神経可塑性 (2009)
  • Author(s): 田村英紀, 塩坂貞夫
  • Journal Title: 分子精神医学誌 第35号 Pages: 68-71
• [Journal Article] 細胞外プロテアーゼと神経可塑性 (2009)
  • Author(s): 田村英紀
  • Journal Title: 分子精神医学誌 35 Pages: 68-71
• [Journal Article] One-chip sensing device (biomedical photonic LSI) enabled to assess hippocampal steep and gradual up-regulated proteolytic activities. (2008)
  • Author(s): Tamura H, Ng DC, Tokuda T, et al. (他7名, 1番目)
  • Journal Title: J Neurosci Meth. 173 Pages: 114-20
  • Peer Reviewed
• [Journal Article] An implantable and fully integrated complementary metal-oxide semiconductor device for in vivo neural imaging and electrical interfacing with mouse hippocampus. (2008)
  • Author(s): Ng DC, Nakagawa T, Mizuno T, et al. (他6名, 6番目)
  • Journal Title: Sens Actuators A 145-6 Pages: 176-86
  • Peer Reviewed
• [Journal Article] Neuropsin (KLK8)-dependent and -independent synaptic tagging in the Schaffer-collateral pathway of mouse hippocampus. (2008)
  • Author(s): Ishikawa Y, Horii Y, Tamura H and Shiosaka S
  • Journal Title: J Neurosci. 28 Pages: 843-9
  • Peer Reviewed
• [Journal Article] Integrated in vivo neural imaging and interface CMOS devices : design, packaging, and implementation. (2008)
  • Author(s): Ng DC, Nakagawa T, Mizuno T, et al. (他6名, 6番目)
  • Journal Title: IEEE Sensors J. 8 Pages: 121-30
  • Peer Reviewed
• [Journal Article] シナプス可塑性におけるプロテオリシス機構の働き (2008)
  • Author(s): 田村英紀
  • Journal Title: 神経化学誌 47 Pages: 21-9
  • NAID: 10021189433
• [Journal Article] One-chip sensing device (biomedical photonic LSI) enable to assess hippocampal steep and gradual up-regulated proteolytic activities (2008)
  • Author(s): H. Tamura
  • Journal Title: J Neurosci. Meth 173 Pages: 114-120
  • Peer Reviewed
• [Journal Article] シナプス可塑性におけるプロテオリシスの働き (2008)
  • Author(s): 田村英紀
  • Journal Title: 神経化学誌 47 Pages: 21-29
• [Journal Article] Neuropsin (KLK8)-dependent and -independent Synaptic tagging in the Schaffer-collateral pathway of mouse hippocampus (2008)
  • Author(s): Y. Ishikawa
  • Journal Title: J Neuroscience 28 Pages: 843-849
  • Peer Reviewed
• [Journal Article] Integrated In Vivo Neural Imaging and Interface CMOS Devices: Design, Packaging, and Implementation (2008)
  • Author(s): DC Ng
  • Journal Title: IEEE Sensors J 8 Pages: 121-130
  • Peer Reviewed
• [Journal Article] Towards a fully-integrated CMOS-based in vivo neural imaging and interface device. (2007)
  • Author(s): Ng DC, Nakagawa T, Mizuno T, et al. (他6名, 6番目)
  • Journal Title: Solid-State Sensors, Actuators and Microsystems Conf. 423-6
• [Journal Article] Development of a fully integrated complementary metal-oxide-semiconductor image sensor-based device for real-time in vivo fluorescence imaging inside the mouse hippocampus. (2007)
  • Author(s): Ng DC, Nakagawa T, Tokuda T, et al. (他5名, 5番目)
  • Journal Title: Jpn J Appl Phys. 46 Pages: 2811-9
  • Peer Reviewed
• [Journal Article] Development of a Fully Integrated Complementary Metal-Oxide-Semiconductor Image Sensor-Based Device for Real-Time In vivo Fluorescence Imaging inside the Mouse Hippocampus (2007)
  • Author(s): DC Ng
  • Journal Title: Jpn. J. Appl. Phys. 46 Pages: 2811-2819
  • Peer Reviewed
• [Journal Article] An implantable and fully-integrated complementary metal-oxide semicon ductor device for in vivo neural imaging and electrical interfacing with the mouse hippocampus
  • Author(s): DC Ng
  • Journal Title: Sensors Actuators A (In press)
  • Peer Reviewed
• [Presentation] ニューロプシンはサイトメガロウイルスプロモーターの活性を増大させる (2008)
  • Author(s): 田村英紀
  • Organizer: 神経化学会
  • Place of Presentation: 富山
  • Year and Date: 2008-09-11
• [Presentation] Neuropsin依存的・非依存的シナプス・タギング (2008)
  • Author(s): 石川保幸
  • Organizer: 神経化学会
  • Place of Presentation: 富山
  • Year and Date: 2008-09-11
• [Presentation] セリンプロテアーゼニューロプシンはGRIN1を特異的に切断する (2008)
  • Author(s): 田村英紀
  • Organizer: 神経科学会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-11
• [Presentation] Neuropsin依存的・非依存的シナプス・タギング (2008)
  • Author(s): 石川保幸
  • Organizer: 神経科学会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-10
• [Presentation] ニューロプシンはサイトメガロウイルスプロモーターの活性を増大させる (2008)
  • Author(s): 田村英紀
  • Organizer: 第51回神経化学会
  • Place of Presentation: 富山
• [Presentation] Neuropsin依存的・非依存的シナプス・タギング (2008)
  • Author(s): 石川保幸
  • Organizer: 第51回神経化学会
  • Place of Presentation: 富山
• [Presentation] セリンプロテアーゼニューロプシンはGRIN1を特異的に切断する (2008)
  • Author(s): 田村英紀
  • Organizer: 第31回神経科学会
  • Place of Presentation: 東京
• [Presentation] Neuropsin依存的・非依存的シナプス・タギング (2008)
  • Author(s): 石川保幸
  • Organizer: 第31回神経科学会
  • Place of Presentation: 東京
• [Presentation] Design and packaging of an implantable CMOS neural imaging and interface device (2007)
  • Author(s): DC Ng
  • Organizer: International Image Sensor Workshop
  • Place of Presentation: Ogunquit
• [Presentation] Towards a fully-integrated CMOS-based in vivo neural imaging and interface device (2007)
  • Author(s): DC Ng
  • Organizer: The 14th Int. Conf. Solid-state Sensors, Actuators and Microsystems
  • Place of Presentation: Lyon
• [Presentation] マウス海馬CA1領域におけるシータバースト刺激後のセリンプロテアーゼin vivoリアルタイムイメージング (2007)
  • Author(s): 田村英紀
  • Organizer: Neuro2007
  • Place of Presentation: 神奈川
• [Presentation] シナプス可塑性関連プロテアーゼニューロプシンによる120kDaタンパク質のプロセシング (2007)
  • Author(s): 松井信樹
  • Organizer: Neuro2007
  • Place of Presentation: 神奈川
• [Presentation] Fusion of CMOS and MEMS technologies for development of a fully integrated and implantable neural imaging and interface device (2007)
  • Author(s): Ng DC
  • Organizer: The 1st Int. Symposium on Information and Computer Elements
  • Place of Presentation: 北九州
• [Presentation] Integration of CMOS and MEMS technologies in the development of a neural imaging and interface device : showcase of an emerging bioimaging technique (2007)
  • Author(s): Ng DC
  • Organizer: Proc. IEEE Custom Integrated Circuits Conference
  • Place of Presentation: San Jose
• [Presentation] Development of a CMOS-based neural imaging and interface device (2007)
  • Author(s): Ng DC
  • Organizer: 2007 Intl. Conf. on Solid State Devices and Materials
  • Place of Presentation: 筑波
• [Presentation] Real time in vivo Long-term Potentiation imaging in the CA1 area of the mouse hippocampus (2007)
  • Author(s): Tamura H
  • Organizer: Society for Neuroscience 2007
  • Place of Presentation: San Diego
• [Presentation] Neuropsin (KLK8)-dependent and -independent Synaptic Tagging in the mouse Schaffer-collateral Pathway (2007)
  • Author(s): Ishikawa Y
  • Organizer: Society for Neuroscience 2007
  • Place of Presentation: San Diego
• [Presentation] マウス海馬CA1領域におけるシータバースト剌激後のセリンプロテアーゼin vivoリアルタイムイメージング (2007)
  • Author(s): 田村 英紀
  • Organizer: Neuro2007
  • Place of Presentation: 神奈川
• [Presentation] シナプス可塑性関連プロテアーゼニューロプシンによる120 kDaタンパク質のプロセシング (2007)
  • Author(s): 松井 信樹
  • Organizer: Neuro2007
  • Place of Presentation: 神奈川
• [Presentation] Integration of CMOS and MEMS technologies in the development of aneural imaging and interface device: showcase of an emerging bioimaging technique (2007)
  • Author(s): Ng DC
  • Organizer: Proc. IEEE Custom Integrated Circuits Conference
  • Place of Presentation: San Jose
• [Remarks]
  • URL: http://bsw3.naist.jp/shiosaka/siosaka.html
• [Remarks]
  • URL: http://bsw3.naist.jp/shiosaka/shiosaka.html