Cbln1, novel synaptogenic factor secreted from presynaptic site〜The mechanism of its acceptance.

• Project/Area Number: 19700349
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Keio University
• Principal Investigator: MATSUDA Keiko Keio University, 医学部, 助教 (40383765)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,600,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥2,300,000 (Direct Cost: ¥2,300,000)
• Keywords: 細胞内情報伝達 / シナプス / 小脳 / プルキンエ細胞 / 可塑性 / 神経栄養因子

Research Abstract

Cbln1分子は小脳顆粒細胞軸索-プルキンエ細胞間でのシナプスの形成・維持と機能的可塑性に必須である分泌性因子である。我々はCbln1全長がリガンドとして働き、その受容体がプルキンエ細胞神経棘上に特異的に局在することを示した。申請者はCbln1と共役して働く受容体候補の膜タンパク質を同定し、この受容体がCbln1によるシナプス形成において必須な受容体複合体の一部であることを見出した。

Research Products

• [Journal Article] The N-terminal domain of GluD2 (GluRδ2) recruits presynaptic terminals and regulates synaptogenesis in the cerebellum in vivo. (2009)
  • Author(s): Kakegawa, W., Miyazaki, T., Kohda, K., Matsuda, K., Emi, K., Motohashi, J., Watanabe, M., Yuzaki, M.
  • Journal Title: Journal of Neuroscience 29 Pages: 5738-5748
  • Peer Reviewed
• [Journal Article] Cbln1 binds to specific postsynaptic sites at parallel fiber-Purkinje cell synapses in the cerebellum. (2009)
  • Author(s): Matsuda K., Kondo, T., Iijima, T., Matsuda, S., Watanabe, M., Yuzaki, M.
  • Journal Title: European Journal of Neuroscience 29 Pages: 707-717
  • Peer Reviewed
• [Journal Article] Cbln1 accumulates and colocalizes with Cbln3 and GluRδ2 at parallel fiber-Purkinje cell synapses in the mouse cerebellum. (2009)
  • Author(s): Miura, E., Matsuda K., Morgan, J.I., Yuzaki, M., Watanabe, M.
  • Journal Title: European Journal of Neuroscience 29 Pages: 693-706
  • Peer Reviewed
• [Journal Article] Cbln1 binds to specific postsynaptic sites at parallel fiber-Purkinje cel synapses in the cerebellum (2009)
  • Author(s): Matsuda, K., Kondo, T., Iijima, T., Matsuda, S., Watanabe, M., Yuzaki, M.
  • Journal Title: European Journal of Neuroscience 29 Pages: 707-717
  • Peer Reviewed
• [Journal Article] Cblnl accumulates and colocalizes with Cbln3 and GluR62 at parallel fiber-Purkinie cell synapses in the mouse cerebellum (2009)
  • Author(s): Miura, E., Matsuda, K., Morgan, J.I., Yuzaki, M., Watanabe, M.
  • Journal Title: European Journal of Neuroscience 29 Pages: 693-706
  • Peer Reviewed
• [Journal Article] The N-terminal domain of GluD2 (GluR82) recruits presynaptic terminals and regulates synaptogenesis in the cerebellum in vivo (2009)
  • Author(s): Kakegawa, W., Miyazaki, T., Kohda, K., Matsuda, K., Emi, K., Motohashi, J., Watanabe, M., Yuzaki
  • Journal Title: Journal of Neuroscience (In press)
  • Peer Reviewed
• [Journal Article] Cbln1 Regulates Rapid Formation and Maintenance of Excitatory Synapses in Mature Cerebellar Purkinje Cells in vitro and in vivo. (2008)
  • Author(s): Ito-Ishida, A., Miura, E., Emi, K., Matsuda K., Iijima, T., Kondo, T., Kohda, K. Watanabe, M., Yuzaki, M.
  • Journal Title: Journal of Neuroscience 28 Pages: 5920-5930
  • Peer Reviewed
• [Journal Article] Accumulation of AMPA Receptors in Autophagosomes in Neuronal Axons Lacking Adaptor Protein AP-4. (2008)
  • Author(s): Matsuda S., Miura E., Matsuda K., Kakegawa W., Kohda K., Watanabe M., Yuzaki M.
  • Journal Title: Neuron 57 Pages: 730-745
  • Peer Reviewed
• [Journal Article] Differential Regulation of Synaptic Plasticity and Cerebellar Motor Learning by the C-terminal PDZ-Binding Motif of GluRdelta2. (2008)
  • Author(s): Kakegawa W., Miyazaki T., Emi K., Matsuda K., Kohda K., Motohash, J., Mishina M., Kawahara S., Watanabe M., Yuzaki M.
  • Journal Title: Journal of Neuroscience 28 Pages: 1460-1468
  • NAID: 120006399304
  • Peer Reviewed
• [Journal Article] Cbln1 Regulates Rapid Formation and Maintenanceof Excitatory Synapses in Mature Cerebellar Purkinje Cells in vitro and in vivo (2008)
  • Author(s): Ito-Ishida, A., Miura E. Emi, K., Matsuda. K. Iijima,T kondo, T., Kohda, E.K. Yuzaki, M.
  • Journal Title: Journal of Neuroscience 28 Pages: 5920-5930
  • Peer Reviewed
• [Journal Article] Characterization of a transneuronal cytokine family Cbln-Regulation of secretion by heteromeric assembly. (2007)
  • Author(s): Iijima I, Miura E, Matsuda K ., Kamekawa Y, Watanabe M, Yuzaki M
  • Journal Title: European Journal of Neuroscience 25 Pages: 1049-1057
  • Peer Reviewed
• [Journal Article] Differential Regulation of Synaptic Plasticity and Cerebellar MotorLeaming by the C-terminal PDZ-Binding Motif of GluRdelta2. (2007)
  • Author(s): Kakegawa W., Miyazaki T., Emi K., Matsuda K, Kohda K., Motohash, J., Mishina M., Kawahara S., Watanabe M., Yuzaki M.
  • Journal Title: Journal of Neuroscience 28 Pages: 1460-1468
  • Peer Reviewed
• [Journal Article] Characterization of a transneuronal cytokine family Cbln-Regulatio of secretion by heteromeric assembly (2007)
  • Author(s): Iijima l, Miura E, Matsuda K ., Kamekawa Y, Watanabe M, Yuzaki M
  • Journal Title: European Journal of Neuroscience 25 Pages: 1049-1057
  • Peer Reviewed
• [Presentation] Mechanism of hexametric Cblnl ligand binding to PSD fraction prepared from cerebellum: implication of Cblnl receptor proteinin Purkinie cells. (2007)
  • Author(s): Matsuda. K., Kondo, .T, Iijima, T., Ishida, A, Yuzaki, M
  • Organizer: 第30回日本神経科学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-09-10
• [Presentation] 神経細胞におけるイオンチャンネル足場タンパク質から核への信号伝達経路の解析 (2007)
  • Author(s): 松田恵子 柚崎通介
  • Organizer: 第30回日本分子生物学会第80回日本生化学会合同大会
  • Place of Presentation: 横浜
• [Presentation] Mechanism of hexametric Cbln1 ligand binding to PSD fraction prepared from cerebellum : implication of Cbln1 receptor protein in Purkinje cells. (2007)
  • Author(s): Matsuda, K., Kondo, T., Iijima, T., Ishida,A., Yuzaki, M.
  • Organizer: The 30th Annual Meeting of the Japan Neuroscience Society.
  • Place of Presentation: 横浜