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Establishment of inbred strains in zebrafish

Research Project

Project/Area Number 19700372
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Laboratory animal science
Research InstitutionNational Institute of Genetics

Principal Investigator

SHINYA Minori  National Institute of Genetics, 系統生物研究センター, 助教 (00372946)

Project Period (FY) 2007 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥3,840,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥540,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywords育種 / 遺伝学 / ゼブラフィッシュ / 近交系 / 育種遺伝
Research Abstract

Zebrafish is a small fresh water fish which is one of the excellent model animals, especially for the developmental biology. Yet, there is no inbred strain in zebrafish. Since the genetic homogeneity of inbred strain improves the precision of analyses, the lack of inbred strains is a big disadvantage for zebrafish. Here, I have tried to inbreed two zebrafish wild-type strains through full sib-pair mating. Though one strain was failed to be succeeded after 13^<th> generation, the other strain has reached to breeding larvae of 20^<th> generation, which fulfills the definition of inbred strains.

Report

(6 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report   Self-evaluation Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (6 results)

All 2010

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (4 results)

  • [Journal Article] Regeneration of spermatogenesis and production of functional sperm by grafting of testicular cell aggregates in zebrafish.2010

    • Author(s)
      Kawasaki, T., Saito, K., Shinya, M., Olsen, L.C., Sakai, N.
    • Journal Title

      Biology of Reproduction 83

      Pages: 533-539

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regeneration of spermatogenesis and production of functional sperm by grafting of testicular cell aggregates in zebrafish.2010

    • Author(s)
      Kawasaki, et.al.
    • Journal Title

      Biology of Reproduction

      Volume: 83 Pages: 533-539

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Trials for establishment of zebrafish inbred strains.2010

    • Author(s)
      Shinya, M., Sakai, N.
    • Organizer
      第16回小型魚類研究会
    • Place of Presentation
      埼玉
    • Related Report
      2010 Final Research Report
  • [Presentation] Trials for establishment of zebrafish inbred strains.2010

    • Author(s)
      Shinya, M., Sakai, N.
    • Organizer
      9th International Zebrafish Genetics and Development Meeting.
    • Place of Presentation
      Madison, Wisconsin, USA
    • Related Report
      2010 Final Research Report
  • [Presentation] Trials for establishment of zebrafish inbred strains2010

    • Author(s)
      M.Shinya, N.Sakai
    • Organizer
      第16回 小型魚類研究会
    • Place of Presentation
      埼玉
    • Related Report
      2010 Annual Research Report
  • [Presentation] Trials for establishment of zebrafish inbred strains2010

    • Author(s)
      M.Shinya, N.Sakai
    • Organizer
      9th International Zebrafish Genetics and Development Meeting
    • Place of Presentation
      Madison Wisconsin
    • Related Report
      2010 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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