Development of hard X-ray-excited confocal microscopy with spatial resolution of 30nm for cellular imaging

• Project/Area Number: 19700380
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Osaka University
• Principal Investigator: MATSUYAMA Satoshi Osaka University, 大学院工学研究科, 助教 (10423196)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,470,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥2,300,000 (Direct Cost: ¥2,300,000)
• Keywords: X線顕微鏡 / 共焦点顕微鏡 / 放射線 / X線,粒子 / X線ミラー / 細胞イメージング / 放射線, X線, 粒子線 / 放射線,X線,粒子

Research Abstract

本研究の目的は,励起光に硬X線を用い検出を可視光で行うことができる硬X線照射型可視共焦点顕微鏡の開発を行い,細胞,組織を可視顕微鏡以上の空間分解能で観察することである.研究を行うに当たって,硬X線集光システムと可視光検出システムを組み合わせた顕微鏡システムを構築した.ヒト培養細胞の細胞質にCdSe-ZnSナノ粒子を染色した試料を作製し観察したところ,500nmの分解能でイメージングすることができた.

Research Products

• [Presentation] Development of Advanced Kirkpatrick-Baez System for X-ray Nano-Imaging (2009)
  • Author(s): M. Fujii, S. Matsuyama, T. Wakioka, H. Mimura, Y. Sano, K. Yamauchi
  • Organizer: First International Symposium on Atomically Controlled Fabrication Technology, p2. 6
  • Year and Date: 2009-02-17
• [Presentation] Development of Advanced Kirkpatrick-Baez System for X-ray Nano-Imaging (2009)
  • Author(s): 藤井正輝
  • Organizer: First international symposium on Atomically Controlled Fabrication Technology
  • Place of Presentation: 大阪府大阪大学
  • Year and Date: 2009-02-16
• [Presentation] Trace element mapping using hard X-ray nanobeam focused by a Kirkpatrick Baez mirror system (2008)
  • Author(s): 松山智至
  • Organizer: European Conference on X-ray Spectrometry
  • Place of Presentation: Croatia Cavtat
  • Year and Date: 2008-06-19
• [Presentation] Trace element mapping using hard X-ray nanobeam focused by a Kirkpatrick Baez mirror system (2008)
  • Author(s): S. Matsuyama, M. Shimura, H. Mimura, M. Fujii, H. Yumoto, S. Handa, T. Kimura, Y. Sano, M. Yabashi, Y. Nishino, K. Tamasaku, T. Ishikawa and K. Yamauchi
  • Organizer: European Conference on X-ray Spectrometry, Abstract p88(011-1)
  • Place of Presentation: Cavtat, Dubrovnik, CROATIA
• [Presentation] 硬X線ナノビームを用いた細胞イメージング (2008)
  • Author(s): 松山智至
  • Organizer: 第7回高度好熱菌丸ごと一匹プロジェクト連携研究会
  • Place of Presentation: 兵庫県SPring-8
• [Presentation] 走査型蛍光X線顕微鏡による細胞内元素分布の測定 (2007)
  • Author(s): 藤井正輝,松山智至, 志村まり,三村秀和,前島一博,片岸恵子,湯本博勝, 半田宗一郎,木村隆志, 佐野泰久, 西野吉則, 玉作賢治, 矢橋牧名, 石川哲也, 山内和人
  • Organizer: 第9回X線結像光学シンポジウム
  • Place of Presentation: 中部大学
  • Year and Date: 2007-11-02
• [Presentation] 走査型蛍光X線顕微鏡による細胞内元素分布の測定 (2007)
  • Author(s): 藤井 正輝
  • Organizer: 第9回X線結像光学シンポジウム
  • Place of Presentation: 中部大学名古屋キャンパス
  • Year and Date: 2007-11-02
• [Presentation] High-resolution and Highly Sensitive Scanning X-ray Fluorescence Micro scopy Using Kirkpatrick-Baez Mirror Optics (2007)
  • Author(s): 松山 智至
  • Organizer: International 21st Century COE Symposium on A tomistic Fabrication Technology 2007
  • Place of Presentation: 大阪大学吹田キャンパス
  • Year and Date: 2007-10-17
• [Presentation] Scanning Hard X-ray Microscopy with Spatial Resolution Better than 50 nm (2007)
  • Author(s): 松山 智至
  • Organizer: NSF-MEXT Exchange program for Japan-US you ng researchers
  • Place of Presentation: 大阪大学吹田キャンパス
  • Year and Date: 2007-06-01
• [Presentation] High-resolution and Highly Sensitive Scanning X-ray Fluorescence Microscopy Using Kirkpatrick-Baez Mirror Optics (2007)
  • Author(s): S. Matsuyama, H. Mimura, K. Katagishi, M. Shimura, M. Fujii, H. Yumoto, S. Handa, T. Kimura, Y. Sano, K. Tamasaku, Y. Nishino, M. Yabashi, T. Ishikawa, K. Yamauchi
  • Organizer: Extended Abstracts of International 21st Century COE Symposium on Atomistic Fabrication Technology 2007, 81-82
  • Place of Presentation: Osaka, Japan
• [Remarks]
  • URL: http://www-up.prec.eng.osaka-u.ac.jp
• [Remarks]
  • URL: http://www-up.prec.eng.osaka-u.ac.jp/