Analyses of cardiac energy metabolism and intracellular Ca2+handling in hypertrophic hearts

• Project/Area Number: 19700385
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Nara Medical University
• Principal Investigator: MISAWA Hiromi Nara Medical University, 医学部, 教務職員 (50281275)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,270,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥2,100,000 (Direct Cost: ¥2,100,000)
• Keywords: 心筋スライス標本 / 心筋細胞内カルシウム動態解析 / X線回折実験 / NMR / 生態情報・計測 / エナジェテクス / 心筋スライス / 心筋細胞内Ca^<2+>動態 / エナジェティクス / 心筋細胞内Ca^2+動態

Research Abstract

心筋スライス標本を用いた心筋細胞内カルシウム動態の計測法を確立し、摘出拍動心標本で観察される現象のメカニズムをより詳細に解明することを目的とした。まず、心筋スライス標本を用い、中心部にあるコア心筋も含めた細胞機能を確認するため、NMRによるリン酸代謝について解析し、心筋細胞は十分な呼吸機能を持つことが確認できた。さらにFluo-3をロードした心筋スライス標本での心筋細胞内カルシウム動態解析法を確立し、病態時の心筋収縮力低下の原因を探った。

Research Products

• [Journal Article] Increased O2 consumption in excitation-contraction coupling in hypertrophied rat heart slices related to increased Na+-Ca2+exchange activity (2009)
  • Author(s): Shimizu J, Yamashita D, Hiromi M, Tohne K, Matsuoka S, Kim B, Takeuchi A, Nakajima-Takenaka C, Takaki M
  • Journal Title: The Journal of Physiological Sciences 59 Pages: 63-74
  • Peer Reviewed
• [Journal Article] Increased O_2 consumption in excitation -contraction coupling in hypertrophied rat heart slices related to increased Na ^+-Ca^<2+> exchange activity (2008)
  • Author(s): Shimizu J
  • Journal Title: The Journal of Physiological Sciences 58(3) Pages: 179-188
  • Peer Reviewed
• [Journal Article] Inhibition of gut pacemaker cell formation from mouse ES cells by the c-kit inhibitor (2007)
  • Author(s): Takaki M, Misawa H, Shimizu J, Kuniyasu H, Horiguchi K
  • Journal Title: Biochemical and Biophysical Research Communications 359 Pages: 354-359
  • Peer Reviewed
• [Journal Article] Heart Slice NMR (2007)
  • Author(s): Uetani T, Yamashita D, Shimizu J, Misawa H, Tatematsu Y, Hamaguchi Y, Kato T, Katanosaka Y, Miyasaka T, Matsubara T, Furukawa K, Murohara T, Takaki M, Nakayama S
  • Journal Title: American Journal of Physiology : Heart and Circulatory Physiology 292
  • Peer Reviewed
• [Journal Article] Heart Slice NMR (2007)
  • Author(s): Uetani T,
  • Journal Title: American Journal of Physiology: Heart and Circulatory Physiology 292 Pages: 1181-1186
  • Peer Reviewed
• [Journal Article] Inhibition of gut pacemaker cell formation from mouse ES cellls by the c-kit inhibitor (2007)
  • Author(s): Takaki M
  • Journal Title: Biochemical and Biophysical Research Communications 359 Pages: 354-359
  • Peer Reviewed
• [Presentation] Increasing heart rate increased residual actin-myosin interaction without raising end-diastolic left ventricular pressure in beating rat heart (2009)
  • Author(s): Tamura Y
  • Organizer: 第73回日本循環器学会総会・学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2009-03-21
• [Presentation] Increasing Heart Rate Incr eased Residual Actin -Myosin Interaction without Raising End-diastolic Left Ventricular Pressure in Beating Rat Heart (2009)
  • Author(s): Yamato Tamura
  • Organizer: 第73回日本循環器学会総会・学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2009-03-21
• [Presentation] Increasing preroad reduced actin -myosin interaction in isolated beating rat whole heart under hypoxia (2009)
  • Author(s): Shimizu J
  • Organizer: Biophysical Society 53^<rd> Annual Meeting
  • Place of Presentation: Boston
  • Year and Date: 2009-03-03
• [Presentation] Increasing preload attenuates crossbridge formation under hypoxia (2009)
  • Author(s): Tamura Y
  • Organizer: Biophysical Society 53^<rd> Annual Meeting
  • Place of Presentation: Boston
  • Year and Date: 2009-03-03
• [Presentation] Increasing preroad reduced actin-myosin interaction in isolated beating rat whole heart under hypoxia (2009)
  • Author(s): Shimizu J
  • Organizer: Biophysical Society 53rd Annual Meeting
  • Place of Presentation: in Boston
  • Year and Date: 2009-03-02
• [Presentation] Transmurally different changes in nano-order myofilament lattice spacing and crossbridge dynamics with increasing left ventricular volume (2009)
  • Author(s): Tamura Y
  • Organizer: Biophysical Society 53rd Annual Meeting
  • Place of Presentation: in Boston
  • Year and Date: 2009-03-02
• [Presentation] Effects of BDNF on regeneration and differentiation of enteric nervous system (2008)
  • Author(s): Miyako Takaki
  • Organizer: 1st World Congress of Regenerative Medicine and Stem Cell
  • Place of Presentation: in Forshan, China
  • Year and Date: 2008-12-02
• [Presentation] 腸管吻合部における壁内神経再生に対するBDNFの効果 (2008)
  • Author(s): 國安弘基
  • Organizer: 第97回日本病理学会
  • Place of Presentation: 金沢
  • Year and Date: 2008-05-15
• [Presentation] ES細胞からの腸管様構造誘導におけるBDNFの効果 (2008)
  • Author(s): 森若優希子
  • Organizer: 第97回日本病理学会
  • Place of Presentation: 金沢
  • Year and Date: 2008-05-15
• [Presentation] ES細胞による腸管様組織構築と運動能の関係 (2008)
  • Author(s): 大森斉
  • Organizer: 第97回日本病理学会
  • Place of Presentation: 金沢
  • Year and Date: 2008-05-15
• [Presentation] Increasing preload attenuates crossbridge formation under hypoxia. (2008)
  • Author(s): himizu J
  • Organizer: 第85回日本生理学会大会
  • Place of Presentation: 京王プラザホテル
  • Year and Date: 2008-03-27
• [Presentation] Decrease in oxygen cost of left ventricular contractility by SERCA2a overexpression (2008)
  • Author(s): Sakata S
  • Organizer: 第85回日本生理学会大会
  • Place of Presentation: 京王プラザホテル
  • Year and Date: 2008-03-27
• [Presentation] Decrease in oxygen cost of left ventricular contractility by SERCA2a overexpression. (2008)
  • Author(s): Sakata S
  • Organizer: 第85回日本生理学会大会
  • Place of Presentation: 東京(京王プラザホテル)
  • Year and Date: 2008-03-27
• [Presentation] Increasing preload attenuates crossbridge formation under hypoxia. (2008)
  • Author(s): Shimizu J
  • Organizer: 第85回日本生理学会大会
  • Place of Presentation: 東京(京王プラザホテル)
  • Year and Date: 2008-03-27
• [Presentation] マウス胚性幹細胞から分化誘導された腸管におけるc-kit陽性細胞(ICC)の形成の対するGIST治療薬グリベックの効果 (2008)
  • Author(s): 高木 都
  • Organizer: 第4回日本消化管学会総会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2008-02-08
• [Presentation] マウス胚性幹細胞から分化誘導された腸管におけるc-kit陽性細胞(ICC)の形成の対するGIST治療薬グリベックの効果 (2008)
  • Author(s): 高木都
  • Organizer: 第4回日本消化管学会総会学術集会トピックフォーラム7(TF-7-7)
  • Place of Presentation: 大阪
  • Year and Date: 2008-02-07
• [Presentation] 低酸素環境下での前負荷増大による心機能低下と心筋分子モータの挙動異常 (2008)
  • Author(s): 柴田 宗孝
  • Organizer: 第18回日本病態生理学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-01-27
• [Presentation] 酸素環境下での前負荷増大による心機能低下と心筋分子モータの挙動異常 (2008)
  • Author(s): 柴田宗孝
  • Organizer: 第18回日本病態生理学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-01-26
• [Presentation] シンポジウム1「ユビキタスペースメーカ」ES腸管における組織構築と運動能 (2007)
  • Author(s): 國安弘基
  • Organizer: 第49回日本平滑筋学会総会
  • Place of Presentation: 橿原
  • Year and Date: 2007-07-05
• [Presentation] ES腸管における組織構築と運動能 (2007)
  • Author(s): 國安 弘基
  • Organizer: 第49回日本平滑筋学会総会
  • Place of Presentation: 奈良
  • Year and Date: 2007-07-05
• [Remarks]
  • URL: http://www.naramed-u.ac.jp/~2phy/Gyoseki.htm
• [Remarks]
  • URL: http://www.naramed-u.ac.jp/~2phy/Gyoseki.htm
• [Remarks]
  • URL: http://www.naramed-u.ac.jp/~2phy/Gyoseki.htm