Analysis of three-dimensional molecular dynamics of PECAM-1 on monocytes and vascular endothelial cells in the early atherogenesis.

• Project/Area Number: 19700391
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Kawasaki Medical School
• Principal Investigator: HASHIMOTO Ken Kawasaki Medical School, 医学部, 助教 (80341080)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,490,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: 動脈硬化 / 血管内皮 / 単球 / PECAM-1 / 分子イメージング / 血管内皮細胞 / 分子ダイナミクス / 浸潤

Research Abstract

動脈硬化初期における単球の血管内皮細胞への接着・浸潤機構には不明点が多い.本研究では,単球及び内皮細胞に発現する蛋白PECAM-1に着目し,その分子動態を直接観察した.単球が内皮細胞間隙を通って浸潤した後,20分程度にわたって,内皮細胞PECAM-1の浸潤スポットへの持続的な集積が認められた.この集積は,単球由来の液性因子や,単球の内皮への接着ではなく,細胞間隙を浸潤することで初めて起こった.このような集積は同一部位での慢性的な単球浸潤を促す可能性があると考えられる.

Research Products

• [Journal Article] Evaluation of Coronary Endothelial Function by Catheter-Type NO Sensor in High-Fat-Diet-Induced Obese Dogs (2009)
  • Author(s): Kume T, Kawamoto T, Okura H, Neishi Y, Hashimoto K, Hayashida A, Watanabe N, Kanda Y, Mochizuki S, Goto M, Yoshida K
  • Journal Title: Circ J. 73(3) Pages: 562-567
  • NAID: 110007126718
  • Peer Reviewed
• [Journal Article] Measurement and finite element modeling of the force balance in the vertical section of adhering vascular endothelial cells (2009)
  • Author(s): Shinji Deguchi, Hiroyuki Fukamachi, Ken Hashimoto, Kazushi Iio, Katsuhiko Tsujioka.
  • Journal Title: Journal of the Mechanical Behavior of Biomedical Materials 2 Pages: 173-185
  • Peer Reviewed
• [Journal Article] Evaluation of Coronary Endothelial Function by Catheter-Type NO Sensor in High-Fat-Diet-Induced Obese Dogs (2009)
  • Author(s): Kume T.
  • Journal Title: Circ J. 73(3) Pages: 562-567
  • Peer Reviewed
• [Journal Article] Measurement and finite element modeling of the force balance in the vertical section of adhering vascular endothelial cells (2009)
  • Author(s): Shinji Deguchi
  • Journal Title: Journal of the Mechanical Behavior of Biomedical Materials 2 Pages: 173-185
  • Peer Reviewed
• [Journal Article] The alteration of a mechanical property of bone cells during the process of changing from osteoblasts to osteocytes (2008)
  • Author(s): Sugawara Y, Ando R, Kamioka H, Ishihara Y, Murshid SA, Hashimoto K, Kataoka N, Tsujioka K, Kajiya F, Yamashiro T, Takano-Yamamoto T
  • Journal Title: Bone 43(1) Pages: 19-24
  • Peer Reviewed
• [Journal Article] Intracellular Ca2+Responses in Cultured Endothelial Cells to Mechanical Stimulation by Laser Tweezers (2008)
  • Author(s): N. Kataoka, K. Hashimoto, S. Kudo, R. Yamaguchi, K. Tsujioka and F. Kajiya
  • Journal Title: Journal of Biomechanical Science and Engineering 3(2) Pages: 116-123
  • NAID: 130000079478
  • Peer Reviewed
• [Journal Article] The alteration of a mechanical property of bone cells during the process of changing from osteoblasts to osteocytes (2008)
  • Author(s): Sugawara Y.
  • Journal Title: Bone 43(1) Pages: 19-24
  • Peer Reviewed
• [Journal Article] Intracellular Ca^<2+> Responses in Cultured Endothelial Cells to Mechanical Stimulation by Laser Tweezers (2008)
  • Author(s): N. Kataoka
  • Journal Title: Journal of Biomechanical Science and Engineering 3(2) Pages: 116-123
  • Peer Reviewed
• [Journal Article] Oxidized LDL specifically promotes the initiation of monocyte invasion during transendothelial migration with upregulated PECAM-1 and downregulated VE-cadherin on endothelial junctions (2007)
  • Author(s): Ken Hashimoto, Noriyuki Kataoka, Emi Nakamura, Katsuhiko Tsujioka, Fumihiko Kajiya
  • Journal Title: Atherosclerosis 194(2)
  • Peer Reviewed
• [Journal Article] [Confocal microscopy], Nippon Rinsho. (2007)
  • Author(s): Hashimoto K, Kataoka N, Kajiya F
  • Journal Title: Japanese journal of clinical medicine 65(2) Pages: 279-86
• [Journal Article] Assessment of the mechanical properties of the nucleus inside a spherical endothelial cell based on microtensile testing. (2007)
  • Author(s): S Deguchi, M Yano, K Hashimoto, H Fukamachi, S Washio, K Tsujioka
  • Journal Title: Journal of Mechanics of Materials and Structures 2(6) Pages: 1087-1102
  • Peer Reviewed
• [Journal Article] Oxidized LDL specifically promotes the initiation of monocyte invasion during transendothelial migration with upregulated PECAM-1 and down regulated VE-cadherin on endothelial junctions. (2007)
  • Author(s): Ken Hashimoto
  • Journal Title: Atherosclerosis 194 Pages: 9-17
  • Peer Reviewed
• [Journal Article] 特集「分子イメージングー現状と展望-」方法論 共焦点顕微鏡法(Confocal Microscopy) (2007)
  • Author(s): 橋本 謙
  • Journal Title: Nippon Rinsho. 65 Pages: 279-286
• [Journal Article] Assessment of the mechanical properties of the nucleus inside a spheric al endothelial cell based on mocrotensile testing. (2007)
  • Author(s): S Deguchi
  • Journal Title: Journal of Mechanics of Materials and Structures 2 Pages: 1087-1102
  • Peer Reviewed
• [Journal Article] Altered Nano/Micro-Order Elasticity of Pulmonary Artery Smooth Muscle Cells of Patients with Idiopathic Pulmonary Arterial Hypertension
  • Author(s): Nakamura K, Shimizu J, Kataoka N, Hashimoto K, Ikeda T, Fujio H, Ohta-Ogo K, Ogawa A, Miura A, Mohri S, Nagase S, Morita H, Kusano KF, Date H, Matsubara H, Mochizuki S, Hashimoto K, Kajiya F, Ohe T
  • Journal Title: Int J Cardiol (in press)
  • Peer Reviewed
• [Journal Article] Altered Nano/Micro-Order Elasticity of Pulmonary Artery Smooth Muscle Cells of Patients with Idiopathic Pulmonary Arterial Hypertension
  • Author(s): Nakamura K.
  • Journal Title: Int J Cardiol (In press)
  • Peer Reviewed
• [Presentation] Increased Cardiomyocyte Elasticity in the Transverse Direction in Hypertrophied Rat Hearts Induced by Chronic β-Adrenergic Stimulation (2009)
  • Author(s): Kazufumi Nakamura, Wakako Sumita, Ken Hashimoto, Daiji Miura, Nobuhiro Nishii, Satoshi Nagase, Hiroshi Morita, Yoshiki Hata, Kengo Kusano, Tohru Ohe
  • Organizer: 第73回日本循環器学会
  • Place of Presentation: 大阪
• [Presentation] Increased Cardiomyocyte Elasticity in the Transverse Direction in Hypertrophied Rat Hearts Indueed by Chronic β-Adrenergic Stimulation (2009)
  • Author(s): Kazufumi Nakamura
  • Organizer: 第73回日本循環器学会
  • Place of Presentation: 大阪
• [Presentation] Local dynamic recruitnent of endothelial PECAM-1 to transmigrating monocytes durign paracellular diapedesis. (2008)
  • Author(s): Ken Hashimoto
  • Organizer: 第85回日本生理学会大会
  • Place of Presentation: 東京
  • Year and Date: 2008-03-26
• [Presentation] Effect of oxidized LDL on monocyte dynamics during transendothelial migration through regulations fo PECAM-1 and VE-cadherin on endothelial junctions. (2008)
  • Author(s): 橋本 謙
  • Organizer: 第33回日本微小循環学会年次総会
  • Place of Presentation: 東京
  • Year and Date: 2008-02-22
• [Presentation] Local Dynamic Recruitment of Endothelial PECAM-1 to Transmigrating Monocytes (2008)
  • Author(s): N. Kataoka, K. Hashimoto, E. Nakamura, K. Hagihara, K. Tsujioka, F Kajiya
  • Organizer: The 13th International Conference on Biomedical Engineering
  • Place of Presentation: Singapore
• [Presentation] 単球(THP-1)の接着による内皮細胞の電気生理学的変化 (2008)
  • Author(s): 氷見直之,濱口愛里,橋本謙,古我知成,辻岡克彦
  • Organizer: 第47回日本生体医工学会大会
  • Place of Presentation: 神戸
• [Presentation] 細胞間隙経由の単球の内皮下浸潤における内皮細胞PECAM-1の単球近傍への局所集積 (2008)
  • Author(s): 橋本謙,片岡則之,中村恵美,萩原喜美子,辻岡克彦,梶谷文彦
  • Organizer: 第47回日本生体医工学会大会
  • Place of Presentation: 神戸
• [Presentation] Increased Cardiomyocyte Stiffness of the Transverse Direction in the Rat Heart with Hypertrophy Induced by Chronic β-Adrenergic Stimulation (2008)
  • Author(s): Wakako Sumita, Kazufumi Nakamura, Daiji Miura, Ken Hashimoto, Satoshi Nagase, Takefumi Oka, Juichiro Shimizu, Kengo Kusano, Miyako Takaki, Tohru Ohe
  • Organizer: 第72回日本循環器学会
  • Place of Presentation: 福岡
• [Presentation] Local dynamic recruitment of endothelial PECAM-1 to transmigrating monocytes during paracellular diapedesis (2008)
  • Author(s): Ken Hashimoto, Noriyuki Kataoka, Emi Nakamura, Kimiko Hagihara, Katsuhiko Tsujioka, and Fumihiko Kajiya
  • Organizer: 第85回日本生理学会大会
  • Place of Presentation: 東京
• [Presentation] Effect of oxidized LDL on monocyte dynamics during transendothelial migration through regulations of PECAM-1 and VE-cadherin on endothelial junctions (2008)
  • Author(s): 橋本謙,片岡則之,辻岡克彦,梶谷文彦
  • Organizer: 第33回日本微小循環学会年次総会
  • Place of Presentation: 東京
• [Presentation] 細胞間隙を通って内皮下へ浸潤する単球近傍への内皮細胞PECAM-1の局所集積 (2008)
  • Author(s): 橋本謙,片岡則之,中村恵美,辻岡克彦,梶谷文彦
  • Organizer: 第20回バイオエンジニアリング講演会
  • Place of Presentation: 東京
• [Presentation] 単球の血管内皮下浸潤におけるPECAM-1動態の生細胞観察系の構築. (2007)
  • Author(s): 橋本 謙
  • Organizer: 第46回日本生体医工学会大会
  • Place of Presentation: 仙台
  • Year and Date: 2007-04-25
• [Presentation] IL-1βと酸化LDLが血管内皮細胞に及ぼす効果 -ナノ・マイクロメカニクスの観点から- (2007)
  • Author(s): 片岡則之
  • Organizer: 第46回日本生体医工学会大会
  • Place of Presentation: 仙台
  • Year and Date: 2007-04-25
• [Presentation] Effects of IL-1β and oxLDL on micromechanics of endothelium related with monocyte transmigration (2007)
  • Author(s): N Kataoka, K Hashimoto, M. R. Williams,S. G. Eskin,, K. Tsujioka, L.V. McIntire, and F. Kajiya
  • Organizer: Biomedical Engineering Society 2007 Annual Fall Meeting
  • Place of Presentation: Los Angels, USA.
• [Presentation] The elastic modulus of osteoblasts and osteocytes (2007)
  • Author(s): Yasuyo Sugawara, Hiroshi Kamioka,Ryoko Ando, Yoshihito Ishihara, Sakhr A Murshid, Ken Hashimoto, Noriyuki Kataoka, Katsuhiko Tsujioka, Fumihiko Kajiya, Takashi Yamashiro, Teruko Takano-Yamamoto
  • Organizer: The American Society for Bone and Mineral Research (ASBMR) 29th Annual Meeting
  • Place of Presentation: Honolulu, Hawaii, USA
• [Presentation] 単球の血管内皮下浸潤におけるPECAM-1動態の生細胞観察系の構築 (2007)
  • Author(s): 橋本謙,片岡則之,中村恵美,辻岡克彦,梶谷文彦
  • Organizer: 第46回日本生体医工学会大会
  • Place of Presentation: 仙台
• [Presentation] IL-1βと酸化LDLが血管内皮細胞に及ぼす効果-ナノ・マイクロメカニクスの観点から- (2007)
  • Author(s): 片岡則之,橋本謙,辻岡克彦,梶谷文彦
  • Organizer: 第46回日本生体医工学会大会
  • Place of Presentation: 仙台
• [Presentation] Effects of IL-1B and oxLDL on micromechanics of endothelium relatedwith monocyte transmigration. (2007)
  • Author(s): N Kataoka
  • Organizer: Biomedical Engineering Society 2007 Annual Fall Meeting
  • Place of Presentation: ロサンゼルス(アメリカ)