Identification of mechanical stimulation to promote osteoanagenesis by a unique three-dimensional scaffold

• Project/Area Number: 19700399
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Tohoku University
• Principal Investigator: MASUDA Taisuke Tohoku University, 大学院歯学研究科, 助教 (30431513)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥570,000); Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,300,000)
• Keywords: 再生医工学材料 / メカニカルストレス / 3次元培養 / 骨芽細胞 / 軟骨細胞 / エラストマー担体 / 細胞間相互作用 / 有限要素解析 / 硬組織再生担体 / リン酸オクタカルシウム / 細胞・組織工学

Research Abstract

本研究は,生体内のストレス環境を模倣した培養システム(メカニカルスティミュレータ)の開発と規則的構造体を有する3次元エラストマー成担体の開発を通して,力学刺激量とそれに対する細胞の増殖・分化誘導条件の最適化を目指した.未分化骨芽細胞を用いた培養の結果,圧縮ひずみ下においてCol1およびOPNの遺伝子発現が抑制されることがわかった.このことからメカニカルストレスによる分化調節・分化維持の可能性が示唆される.

Research Products

• [Journal Article] Molecular Mechanisms of mechanical Stress Response during Chondrogenesis (2009)
  • Author(s): I. Takahashi, T. Masuda, et al.
  • Journal Title: Journal of Boimechanical Science and Engineerng 4(印刷中)
  • NAID: 130000129002
  • Peer Reviewed
• [Journal Article] Molecular Mechanisms of mechanical Stress Response during Chondrogenesis (2009)
  • Author(s): Ichiro Takahashi, Taisuke Masuda, et.al.
  • Journal Title: Journal of Biomechanical Science and Engineering Vol.4, No.3(印刷中)
  • NAID: 130000129002
  • Peer Reviewed
• [Journal Article] 局所環境が硬組織形成細胞の分化に及ぼす影響について (2008)
  • Author(s): 鈴木治, 益田泰輔, 高橋一郎
  • Journal Title: 生体医工学 46 Pages: 414-418
  • NAID: 110006989895
  • Peer Reviewed
• [Journal Article] Development of cell culture system loading cyclic mechanical strain to chondrogenic cells (2008)
  • Author(s): T. Masuda, I. Takahashi, et al.
  • Journal Title: J. Biotechnol 133 Pages: 231-238
  • Peer Reviewed
• [Journal Article] Development of cell culture system loading cyclic mechanical strain to chondrogenic cells (2008)
  • Author(s): Taisuke Masuda
  • Journal Title: Journal of Biotechnology 133 Pages: 231-238
  • Peer Reviewed
• [Journal Article] Fabrication of PDMS scaffold with controlled configuration (2007)
  • Author(s): Taisuke Masuda
  • Journal Title: Proceedings of 2007 International Symposium on Micro-Nano Mechatronics and Human Science Pages: 362-367
  • Peer Reviewed
• [Presentation] Effects of Mechanical Strain on Differentiation of Osteoblastic Cells (2008)
  • Author(s): A Mastui, et al.
  • Organizer: 2008 International Symosium on Micro-Nano Mechatronics and Human Science
  • Place of Presentation: Nagoya
  • Year and Date: 2008-11-08
• [Presentation] Effects of Mechanical Strain on Differentiation of Osteoblastic Cells (2008)
  • Author(s): Aritsune Matsui
  • Organizer: 2008 International Symposium on Micro-Nano Mechatronics and Human Science
  • Place of Presentation: Nagoya
  • Year and Date: 2008-11-08
• [Presentation] Characterization of Three-Dimensional Elastomer Scaffolds for Mechanical Stimulation Culture (2008)
  • Author(s): T. Masuda, et al.
  • Organizer: 8th World Biomaterials Congress
  • Place of Presentation: Amsterdam
  • Year and Date: 2008-05-29
• [Presentation] Characterization of Three-Dimensional Elastomer Scaffolds for Mechanical Stimulation Culture (2008)
  • Author(s): Taisuke Masuda
  • Organizer: 8th World Biomaterials Congress
  • Place of Presentation: Amsterdamオランダ
  • Year and Date: 2008-05-29
• [Presentation] Fabrication of PDMS scaffold with controlled configuration (2007)
  • Author(s): T. Masuda, et al.
  • Organizer: 2007 International Symosium on Micro-Nano Mechatronics and Human Science
  • Place of Presentation: Nagoya
  • Year and Date: 2007-11-11
• [Presentation] 勾配量メカニカルストレス培養システムの開発 (2007)
  • Author(s): 益田泰輔, ほか
  • Organizer: 第46回日本生体医工学会大会
  • Place of Presentation: 仙台
  • Year and Date: 2007-04-26
• [Presentation] 勾配量メカニカルストレス培養システムの開発 (2007)
  • Author(s): 益田泰輔
  • Organizer: 第46回日本生体医工学会大会
  • Place of Presentation: 宮城県・仙台市
  • Year and Date: 2007-04-26
• [Book] Interface Oral Health Science 2007, Eds. M. Watanabe and O. Okuno (2007)
  • Author(s): T. Masuda, et al. (分担執筆)
  • Publisher: Springer
• [Book] Interface Oral Health Science 2007, Eds. M. Watanabe and O. Okuno (2007)
  • Author(s): Taisuke Masuda(分担執筆)
  • Publisher: Springer
• [Remarks]
  • URL: http://www-kiso.dent.tohoku.ac.jp/cfe/index.html
• [Remarks]
  • URL: http://www-kiso.dent.tohoku.ac.jp/cfe/index.html
• [Remarks]
  • URL: http://www-kiso.dent.tohoku.ac.jp/cfe/index.html