Newly molecular design of outermost surface functionalized thermoresponsive nano-micelles for drug carrier systems

• Project/Area Number: 19700407
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Tokyo Women's Medical University
• Principal Investigator: NAKAYAMA Masamichi Tokyo Women's Medical University, 医学部, 講師 (00338980)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,420,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2007: ¥1,600,000 (Direct Cost: ¥1,600,000)
• Keywords: 高分子ミセル / 薬物伝達システム / RAFT重合 / 薬物キャリア / 温度応答性 / 表面機能化 / ポリ(N-イソプロピルアクリルアミド) / ブロックコポリマー / pH応答性 / ドラッグデリバリーシステム / ポリ(ルイソプロピルアクリルアミド) / 蛍光標識 / 細胞内移行

Research Abstract

分子量制御した温度応答性高分子鎖と疎水性高分子鎖からなるブロックコポリマーを合成し、種々の官能基を温度応答性高分子鎖末端に導入することで、表面機能型温度応答性高分子ミセルを作製した。ミセル表面に蛍光基を導入することにより、ミセルの相転移温度以上で細胞内への移行が著しく増大することを明らかにした。また、スルホンアミド誘導体を導入することで、生体内の低pH 環境に応答して相転移を引き起こす薬物キャリアを構築した。

Research Products

• [Journal Article] Molecular Design of Outermost Surface Functionalized Thermoresponsive Polymeric Micelles with Biodegradable Cores (2008)
  • Author(s): Jun Akimoto, Masamichi Nakayama, Kiyotaka Sakai, Teruo Okano
  • Journal Title: J. Polym. Sci., Part A, Polym. Chem. 46 Pages: 7127-7137
  • Peer Reviewed
• [Journal Article] Unique Thermoresponsive Polymeric Micelle Behavior via Cooperative Polymer Corona Phase Transitions (2008)
  • Author(s): Masamichi Nakayama, Teruo Okano
  • Journal Title: Macromolecules 41 Pages: 504-507
  • Peer Reviewed
• [Journal Article] Molecular Design of Outermost Surface Functionalized Thermo-responsive Polymeric Micelles with Biodegradable Cores (2008)
  • Author(s): J. Akimoto, M. Nakayama, K. Sakai, T. Okano
  • Journal Title: J. Polvm. Polvm., Part A, Polym. Chem 46 Pages: 7127-7137
  • Peer Reviewed
• [Journal Article] Unique Thermoresponsive Polymeric Micelle Behavior via Cooperative Polymer Corona Phase Transitions (2008)
  • Author(s): M. Nakayama and T. Okano
  • Journal Title: Macromolecules 41 Pages: 504-507
  • Peer Reviewed
• [Journal Article] Thermal modulation of intracellular drug distribution using thermoresponsive polymeric micelles (2007)
  • Author(s): Masamichi Nakayama, Joo Eun Chung, Takanari Miyazaki, Masayuki Yokoyama, Teruo Okano
  • Journal Title: Reactive & Functional Polymers 67 Pages: 1398-1407
  • Peer Reviewed
• [Journal Article] Thermal modulation of intracellular drug distribution using thermoresponsive poly meric micelles (2007)
  • Author(s): M. Nakayama, J. E. Chung, T. Miyazaki, M. Yokoyama, T. Okano
  • Journal Title: Reactive & Functional Polymers 67 Pages: 1398-1407
  • Peer Reviewed
• [Journal Article] Temperature-Induced Intracellular Uptake of Thermoresponsive Polymeric
  • Author(s): Jun Akimoto, Masamichi Nakayama, Kiyotaka Sakai, Teruo Okano
  • Journal Title: Biomacromolecules (in press)
  • Peer Reviewed
• [Journal Article] Temperature-Modulated Intracellular Localization of Thermores-nonsive Polymeric Micelles
  • Author(s): J. Akimoto, M. Nakayama, K. Sakai, T. Okano
  • Journal Title: Biomacromolecules (accepted)
  • Peer Reviewed
• [Presentation] Molecular Design of Thermoresponsive Polymeric Micelles with Surface pH-Responsive Sulfonamide Moieties (2009)
  • Author(s): Masamichi Nakayama, Yayoi Kawahara, Hideko Kanazawa, Teruo Okano
  • Organizer: 14th International Symposium on Recent Advances in Drug Delivery Systems
  • Place of Presentation: ソルトレイクシティ(USA)
  • Year and Date: 2009-02-17
• [Presentation] Molecular Design of Thermoresponsive Polymeric Micelles with Surface DH-Responsive Sulfonamide Moieties (2009)
  • Author(s): M. Nakayama, Y. Kawahara, H. Kanazawa, T. Okano
  • Organizer: 14th International Symposium on Recent A dvances in Drug Delivery Systems
  • Place of Presentation: ソルトレイクシティ(アメリカ)
  • Year and Date: 2009-02-17
• [Presentation] Surface Functionalization and Characterization of Thermoresponsive Polymeric Micelles (2007)
  • Author(s): Masamichi Nakayama, Jun Akimoto, Kiyotaka Sakai, Teruo Okano
  • Organizer: 1st Asian Biomaterials Congress
  • Place of Presentation: 茨城県つくば市
  • Year and Date: 2007-12-06