| Budget Amount *help |
¥3,930,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥630,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Research Abstract |
It is not yet clear that how mechanical stimuli during muscle contraction, such as stretch and pressure stresses, affect skeletal myocyte differentiation. The purpose of this study was to examine the effects of pure pressure stimulus on muscle differentiation in differentiating myoblasts. Atmospheric pressure at 160 mmHg, which is equivalent to calf muscle intramuscular pressure during locomotion, was applied to early phase of L6 myoblast differentiation. Compared to controls kept under normal pressure, the number of fused myoblasts reduced after pressurization. Myogenin and myosin heavy chain proteins in the pressurized cells were lowered. These results suggest that elevated intramuscular pressure may suppress myogenic differentiation from myoblasts to myotubes in contracted skeletal muscles.
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