Inhibitory effect of food factors on cytotoxic ozone-induced inflammation

• Project/Area Number: 19700592
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Eating habits, studies on eating habits
• Research Institution: University of Shizuoka
• Principal Investigator: MIYOSHI Noriyuki University of Shizuoka, 食品栄養科学部, 助教 (70438191)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,660,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2007: ¥2,100,000 (Direct Cost: ¥2,100,000)
• Keywords: 健康と食生活 / 生活習慣病 / 酸化ストレス / 分析化学 / 細胞・組織

Research Abstract

本研究では、動脈硬化病巣やアルツハイマー脳患者から検出されたオゾン酸化コレステロール(athgeronal-AおよびB)の生物化学的解析を行い、atheronalsが有する強力な細胞毒性活性を明らかにした。また培養細胞、試験管内反応よりatheornals生成分子機構における食品成分の抑制効果について検討を行った。

Research Products

• [Journal Article] Formation of cholesterol ozonolysis products through an ozone-free mechanismmediated by themyeloperoxidase-H(2)O(2)-chloride system. (2009)
  • Author(s): Tomono S, Miyoshi N, Sato K,Ohba Y, Ohshima H.
  • Journal Title: Biochem. Biophys. Res. Commun (in press)
• [Journal Article] Formation of cholesterol ozonolysis products through an ozone-free mechanism mediated by the myeloperoxidase-H(2)O(2)-chloride system. (2009)
  • Author(s): Tomono S, Miyoshi N, Sato K, Ohba Y, Ohshima H.
  • Journal Title: Biochem. Biophys. Res. Commun. (印刷中)
  • Peer Reviewed
• [Journal Article] ATP depletion alters the mode of cell death induced by benzyl isothiocyanate. (2008)
  • Author(s): Miyoshi N, Watanabe E, Osawa T, Okuhira M, Murata Y, Ohshima H, Nakamura Y
  • Journal Title: Biochim. Biophys. Acta 1782 Pages: 566-573
• [Journal Article] ATP depletion alters the mode of cell death induced by benzyl isothio cyanate. (2008)
  • Author(s): Miyoshi N, Watanabe E, Osawa T, Okuhira M, Murata Y, Ohs hima H, Nakamura Y.
  • Journal Title: Biochim. Biophys. Acta. 1782 Pages: 566-73
  • Peer Reviewed
• [Presentation] 活性化好中球が産生するオゾン様活性酸素による酸化コレステロールの生成 (2008)
  • Author(s): 伴野勧、塩川秀美、三好規之、望月和樹、合田敏尚、大島寛史
  • Organizer: 第81回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] アセトアルデヒド曝露マーカーとしてのタンパク質中のNε-エチルリジンのNCI-GC/MSによる分析:ヒトロ腔内剥離細胞における検出 (2008)
  • Author(s): 馬渕良太、栗田亜矢、三好規之、菅敏幸、雨谷敬史、大島寛史
  • Organizer: 日本環境変異原学会第37回大会
  • Place of Presentation: 沖縄
• [Presentation] AGEs由来アルギニン誘導体によるアルギニン代謝に関与する酵素活性に及ぼす影響 (2008)
  • Author(s): 頼盈伶、青山沙絵、永井竜児、三好規之、大島寛史
  • Organizer: 第18回日本メイラード学会
  • Place of Presentation: 東京
• [Presentation] Inhibitory effects of advanced glycation end products-related arginine derivatives on enzymatic activities of arginine-metabolizing enzymes. (2008)
  • Author(s): Lai Y-L, Aoyama S, Nagai R, Miyoshi N, Ohshima H
  • Organizer: The International Symposium on Lipid Peroxidation 2008
  • Place of Presentation: Karuizawa, Nagano, Japan
• [Presentation] Cytotoxic effects of cholesterol ozonolysis products on HL-60 cells (2008)
  • Author(s): Tomono S,Miyoshi N, Ohshima H.
  • Organizer: The International Symposium on Lipid Peroxidation 2008.
  • Place of Presentation: Karuizawa, Nagano, Japan
• [Presentation] Foemation of cholesterol ozonolysis products by differentiated neutrophil-like HL-60cells activated with phorbol myristate acetate. (2008)
  • Author(s): Tomono S, Shiokawa H, Miyoshi N,Ohshima H
  • Organizer: The International Symposium on Lipid Peroxidation 2008.
  • Place of Presentation: Karuizawa, Nagano, Japan
• [Presentation] Increased expression and enzymatic activities of dimethylarginine dimethylaminohydrorase in cultured human cancerous and non-cancerous cells (2008)
  • Author(s): Aoyama S, Lai Y-L, Miyoshi N, Ohshima H
  • Organizer: International Conference Biology, Chemistry and Therapeutic Applications ofNitric Oxide
  • Place of Presentation: Bregenz, Austria
• [Presentation] Inhibitory effects of advanced glycation end products-related arginine derivatives on enzymatic activities of three isoforms of nitric oxide synthase. (2008)
  • Author(s): Lai Y-L, Aoyama S, Nagai R, Miyoshi N, Ohshima, H
  • Organizer: Fifth International Conference Biology, Chemistry and Therapeutic Applications ofNitric Oxide
  • Place of Presentation: Bregenz, Austria
• [Presentation] 好中球様分化HL-60細胞におけるオゾン酸化コレステロールの生成 (2008)
  • Author(s): 伴野勧、三好規之、石井剛志、大島寛史
  • Organizer: 第61回日本酸化ストレス学会学術集会
  • Place of Presentation: 京都
• [Presentation] Alpha-tocopherol induces procaspase-3 expression mediated by the transcriptional factor Spl. (2008)
  • Author(s): Miyoshi N, Nakamura Y, Ohshima H
  • Organizer: Experimental Biology
  • Place of Presentation: San Diego, CA, USA.
• [Presentation] Alpha-tocopherol induces procaspase-3 expression mediated by the tran scriptional factor Spl (2008)
  • Author(s): Miyoshi N, Nakamura Y, Ohshima H.
  • Organizer: Experimental Biology
  • Place of Presentation: San Diego, CA, USA.
• [Presentation] Inhibitory effects of advanced glycation end products-related arginine derivatives on enzymatic activities of three isoforms of nitric oxide sy nthase (2008)
  • Author(s): Lai Y-L, Aoyama S, Nagai R, Miyoshi N, Ohshima, H.
  • Organizer: Fifth International Conference Biology, Chemistry and Theraneutic Applications of Nitric Oxide
  • Place of Presentation: Bregenz, Austria.
• [Presentation] Increased expression and enzymatic activities of dimethylarginine dimet hylaminohydrorase in cultured human cancerous and non-cancerous cell (2008)
  • Author(s): Aoyama S, Lai Y-L, Miyoshi N, Ohshima H.
  • Organizer: Fifth International Conference Biology, Chemistry and Theraneutic Annlications of Nitric Oxide
  • Place of Presentation: Bregenz, Austria.
• [Presentation] Foemation of cholesterol ozonolysis products by differentiated neutroph it-like HL-60 cells activated with nhorbol mvristate acetate (2008)
  • Author(s): Tomono S, Shiokawa H, Miyoshi N, Ohshima H.
  • Organizer: The International Symposium on Lipid Peroxidati on 2008
  • Place of Presentation: Karuizawa, Nagano, Japan
• [Presentation] Cytotoxic effects of cholesterol ozonolysis products on HL-60 cells (2008)
  • Author(s): Tomono S, Miyoshi N, Ohshima H.
  • Organizer: The International Symposium on Lipid Peroxidati on 2008
  • Place of Presentation: Karuizawa, Nagano, Japan
• [Presentation] Inhibitory effects of advanced glycation end products-related arginine derivatives on enzymatic activities of arginine-metabolizing enzymes (2008)
  • Author(s): Lai Y-L, Aoyama S, Nagai R Mivoshi N. Ohshima H.
  • Organizer: The International Symposium on Lipid Peroxidati on 2008
  • Place of Presentation: Karuizawa, Nagano, Japan.
• [Presentation] アセトアルデヒド曝露マーカーとしてのタンパク質中のN^5-エチルリジンのNCI-GC/MSによる分析 : ヒトロ腔内剥離細胞における検出 (2008)
  • Author(s): 馬渕良太、栗田亜矢、三好規之、菅敏幸、雨谷敬史、大島寛史
  • Organizer: 日本環境変異原学会第37回大会
  • Place of Presentation: 沖縄
• [Presentation] Cholesterol ozonolysis products as a possible biomarker for metabolic syndrome (2008)
  • Author(s): Tomono S., Iwabuchi T., Yasue Y., Niizuma S., Miyoshi M., Ohshima H.
  • Organizer: International symposium on Biomarkers of Oxidative Stress in Health and Diseases
  • Place of Presentation: Osaka,Japan
• [Presentation] オゾン酸化コレステロールによるHL-60細胞の細胞傷害分子機構 (2007)
  • Author(s): 伴野 勧、新妻 聖子、岩渕 智恵、安江 祐佳墨、三好 規之、大島 寛史
  • Organizer: 第80回日本生化学会大会(Biochemistry and Molecular Biology 2007)
  • Place of Presentation: 横浜市(パシフィコ横浜)
• [Book] Chemoprevention of Cancer and DNA Damage by Dietary Factors (2009)
  • Author(s): Ohshima H, Tomono S, Lai Y-L,Miyoshi N
  • Publisher: WILEY-BLACKWELL
• [Book] Chemoprevention of Cancer and DNA Damage by Dietary Factors (2009)
  • Author(s): Ohshima H, Tomono S, Lai Y-L, Miyoshin(分担)
  • Total Pages: 787
  • Publisher: WILEY-BLACKWELL
• [Book] 酸化ストレスの医学 (2008)
  • Author(s): 大島寛史、三好規之
  • Publisher: 診断と治療社
• [Book] がん予防『酸化ストレスの医学』 (2008)
  • Author(s): 大島寛史、三好規之(分担)
  • Total Pages: 384
  • Publisher: 診断と治療社
• [Remarks]
  • URL: http://sfns.u-shizuoka-ken.ac.jp/cellbioc/