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Detection of guanine oxidation products which cause G→C transversions.

Research Project

Project/Area Number 19710052
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Risk sciences of radiation/Chemicals
Research InstitutionTokushima Bunri University

Principal Investigator

KINO Katsuhito  Tokushima Bunri University, 香川薬学部・薬科学科, 講師 (70360534)

Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥3,780,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥480,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2007: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordsグアニン酸化損傷 / 紫外線 / G→Cトランスバージョン
Research Abstract

One of guanine oxidation products, oxazolone, causes G→C transversions. In this research, we intended to find aptamers binding oxazolone. Aptamaer binding oxazolone-resin were found but it can bind weakly with free oxazolone. Then some knowledge of finding aptamers binding oxazolone was obtained.

Report

(3 results)
  • 2009 Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (58 results)

All 2010 2009 2008 2007 Other

All Journal Article (18 results) (of which Peer Reviewed: 10 results) Presentation (30 results) Book (2 results) Remarks (8 results)

  • [Journal Article] The oxidation of 8-oxo-7, 8-dihydroguanine by iodine.2010

    • Author(s)
      Kino, K., Morikawa, M., Kobayashi, T., Kobayashi T., Komori, R., Sei, Y., Miyazawa, H.
    • Journal Title

      Bioorg.Med.Chem.Lett. 20(in press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] フラビン誘導体の光分解生物とグアニン光酸化反応2010

    • Author(s)
      喜納克仁、小林輝彦、有馬英治、小森理絵、小林隆信、宮澤宏
    • Journal Title

      ビタミン 84

      Pages: 59-60

    • Related Report
      2008 Annual Research Report
  • [Journal Article] Eukaryotic DNA polymerasesα, βandεincorporate guanine opposite 2, 2, 4-triamino-5(2H)-oxazolone.2009

    • Author(s)
      Kino, K. *, Sugasawa, K., Mizuno, T., Bando, T., Sugiyama, H., Akita, M., Miyazawa, H., Hanaoka, F.
    • Journal Title

      ChemBioChem 10

      Pages: 2613-2616

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Photoirradiation products of flavin derivatives, and the effects of photooxidation on guanine.2009

    • Author(s)
      Kino, K. *, Kobayashi, T., Arima, E., Komori, R., Kobayashi T., Miyazawa, H.
    • Journal Title

      Bioorg. Med. Chem. Lett. 19

      Pages: 2070-2074

    • NAID

      110007575037

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] A new small molecule that directly inhibits the DNA binding of NF-kB.2009

    • Author(s)
      Kobayashi, T., Yoshimori, A., Kino, K., Komori, R., Miyazawa, H., Tanuma, S.
    • Journal Title

      Bioorg. Med. Chem. 17

      Pages: 5293-5297

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] The oxidation of 2'-deoxy-8-oxoguanosine by iodine.2009

    • Author(s)
      Morikawa, M., Kobayashi, T., Kobayashi, T., Komori, R., Sei, Y., Miyazawa, H., Kino, K.
    • Journal Title

      Nucleic Acids Sym. Ser. 53

      Pages: 219-220

    • Related Report
      2009 Final Research Report
  • [Journal Article] A new preparations of guanine photo-oxidation products.2009

    • Author(s)
      Kino, K. *, Morikawa, M., Kobayashi, T., Kobayashi T., Komori, R., Sei, Y., Miyazawa, H.
    • Journal Title

      Photomed. Photobiol. 31

      Pages: 5-6

    • Related Report
      2009 Final Research Report
  • [Journal Article] Eukaryotic DNA polymerases alpha, beta and epsilon incorporate guanine opposite 2 ,2, 4-triamino-5(2H)-oxazolone.2009

    • Author(s)
      Kino, K., Sugasawa, K., Mizuno, T., Bando, T., Sugiyama, H., Akita, M., Miyazawa, H., Hanaoka, F.
    • Journal Title

      ChemBioChem 10

      Pages: 2613-2616

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Photoirradiation products of flavin derivatives, and the effects of photooxidation on guanine.2009

    • Author(s)
      Kino, K.. Kobayashi, T., Arima, E., Komori, R., Kobayashi T., Miyazawa, H.
    • Journal Title

      Bioorg.Med.Chem.Lett. 19

      Pages: 2070-2074

    • NAID

      110007575037

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A new small molecule that directly inhibits the DNA binding of NF-kB.2009

    • Author(s)
      Kobayashi, T., Yoshimori, A., Kino. K.., Komori, R., Miyazawa, H., Tanuma, S.
    • Journal Title

      Bioorg.Med.Chem. 17

      Pages: 5293-5297

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The oxidation of 2'-deoxy-8-oxoguanosine by iodine.2009

    • Author(s)
      Morikawa, M., Kobayashi, T., K obayashi, T., Komori, R., Sei, Y., Miyazawa, H., Kino, K..
    • Journal Title

      Nucleic Acids Sym.Ser. 53

      Pages: 219-220

    • Related Report
      2008 Annual Research Report
  • [Journal Article] A new preparations of guanine photo-oxidation products.2009

    • Author(s)
      Kino, K., Morikawa, M., Kobayashi, T., Kobayashi T., Komori, R ., Sei, Y., Miyazawa, H.
    • Journal Title

      Photomed. Photobiol. 31

      Pages: 5-6

    • Related Report
      2008 Annual Research Report
  • [Journal Article] Identification of RNA binding specificity for the TET-family proteins.2008

    • Author(s)
      Takahama, K., Kino, K., Arai, S., Kurokawa, R., Oyoshi, T.
    • Journal Title

      Nucleic Acids Symp. Ser. 52

      Pages: 213-214

    • Related Report
      2009 Final Research Report
  • [Journal Article] Sequence dependence of fluorescence emission and quenching of doubly thiazole orange labeled DNA: effective design of a hybridization-sensitive probe.2008

    • Author(s)
      Ikeda S., Kubota T., Kino K., Okamoto A.
    • Journal Title

      Bioconjugate Chem. 19

      Pages: 1719-1725

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Detection of base pairing of an oxidatively damaged guanine using colloidal stability change of DNA-linked polymer micelles.2007

    • Author(s)
      Takarada T, Imaizumi E, Kino K, Yoshimoto K, Ogasawara S, Kanayama N, Maeda M.
    • Journal Title

      Nucleic Acids Symp. Ser. 51

      Pages: 305-6

    • Related Report
      2009 Final Research Report
  • [Journal Article] User-freiendly synthesis and photoirradiation of a flavin-linked oligomer.2007

    • Author(s)
      Kino K., Miyazawa H., Sugiyama H.
    • Journal Title

      Genes and Environment 29

      Pages: 23-28

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Detection of base pairing of an oxidatively damaged guanine using Colloidal stability change of DNA-linked polymer micelles2007

    • Author(s)
      T. Takarada, E. Imaizumi, K. Kino.K. Yoshimoto, S. Ogasawara, N.Kanayama, M. Maeda
    • Journal Title

      NUCLEIC ACIDS SYMP SER 51

      Pages: 305-306

    • Related Report
      2007 Annual Research Report
  • [Journal Article] The Oxidation of 8-Oxo-7, 8-dihydroguanine by Iodine

    • Author(s)
      Kino, K. *, Morikawa, M., Kobayashi, T., Kobayashi T., Komori, R., Sei, Y., Miyazawa, H.
    • Journal Title

      Bioorg. Med. Chem. Lett. 2010(in press)

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Presentation] オキサゾロンの点突然変異能2010

    • Author(s)
      喜納克仁
    • Organizer
      日本化学会第90春季年会(2010)
    • Place of Presentation
      大阪
    • Year and Date
      2010-03-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] ヨウ素による80xoG酸化生成物の分子量測定2010

    • Author(s)
      森川雅行、小林輝彦、小林隆信、小森理絵、清悦久、宮澤宏、喜納克仁
    • Organizer
      日本化学会第90春季年会(2010)
    • Place of Presentation
      大阪
    • Year and Date
      2010-03-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] オキサゾロンの点突然変異能2010

    • Author(s)
      喜納克仁
    • Organizer
      日本化学会第90春季年会
    • Place of Presentation
      近畿大学
    • Related Report
      2009 Final Research Report
  • [Presentation] ヨウ素による8oxoG酸化生成物の分子量測定2010

    • Author(s)
      森川雅行、小林輝彦、小林隆信、小森理絵、清悦久、宮澤宏、喜納克仁
    • Organizer
      日本化学会第90春季年会
    • Place of Presentation
      近畿大学
    • Related Report
      2009 Final Research Report
  • [Presentation] ヨウ素による8オキソグアニンの酸化反応2009

    • Author(s)
      喜納克仁、森川雅行、小林輝彦、小森理絵、小林隆信、清悦久、宮澤宏
    • Organizer
      第12回ヨウ素学会シンポジウム
    • Place of Presentation
      千葉
    • Year and Date
      2009-10-29
    • Related Report
      2008 Annual Research Report
  • [Presentation] The oxidation of 2'-deoxy-8-oxoguanosine by iodine.2009

    • Author(s)
      Morikawa, M., Kobayashi, T.Kobayashi, T., Komori, R., Sei, Y., Miyazawa, H., Kino, K
    • Organizer
      The 6th International Symposium on Nucleic Acids Chemistry, 2009 (36th Symposium on Nucleic Acids Chemistry)
    • Place of Presentation
      Takayama, Japan
    • Year and Date
      2009-09-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] The oxidation of 2 ' -deoxy-8-oxoguanosine by iodine.2009

    • Author(s)
      Morikawa M, Kobayashi T, Kobayashi T, Komori R, Sei Y, Miyazawa H, Kino K.
    • Organizer
      6th International Symposium on Nucleic Acids Chemistry
    • Place of Presentation
      高山
    • Related Report
      2009 Final Research Report
  • [Presentation] Ab initio calculation of base pairs containing oxazolone.2009

    • Author(s)
      Kino K, Kobayashi T., Komori R., Miyazawa H.
    • Organizer
      日本環境変異原学会第38回大会
    • Place of Presentation
      静岡
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] ヨウ素による8オキソグアニンの酸化反応2009

    • Author(s)
      喜納克仁、森川雅行、小林輝彦、小森理絵、小林隆信、清悦久、宮澤宏
    • Organizer
      第12回ヨウ素学会シンポジウム
    • Place of Presentation
      千葉大学
    • Related Report
      2009 Final Research Report
  • [Presentation] グアニン光酸化生成物の新規生成反応とその解析2009

    • Author(s)
      喜納克仁、森川雅行、小林輝彦、小森理絵、小林隆信、清悦久、宮澤宏
    • Organizer
      第31回日本光医学・光生物学会
    • Place of Presentation
      大阪
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] ルミフラビンの新しい作成法2009

    • Author(s)
      小林輝彦、喜納克仁、小林隆信、小森理絵、宮澤宏
    • Organizer
      日本化学会第89春季年会
    • Place of Presentation
      千葉
    • Related Report
      2009 Final Research Report
  • [Presentation] 核酸結合タンパク質EWSのDNA結合性の解析2009

    • Author(s)
      高濱謙太朗・喜納克仁・内山裕美子・長橋真弓・大吉崇文
    • Organizer
      日本化学会第89春季年会
    • Place of Presentation
      千葉
    • Related Report
      2009 Final Research Report
  • [Presentation] ルミクロームの新しい作成法2009

    • Author(s)
      森川雅行、小林輝彦、喜納克仁、小林隆信、小森理絵、宮澤宏
    • Organizer
      日本薬学会第129年会
    • Place of Presentation
      京都
    • Related Report
      2009 Final Research Report
  • [Presentation] NF-kBのDNA結合を阻害する低分子化合物の同定2009

    • Author(s)
      小林隆信、吉森篤史、角田綾子、喜納克仁、小森理絵、田沼靖一、宮澤宏
    • Organizer
      日本薬学会第129年会
    • Place of Presentation
      京都
    • Related Report
      2009 Final Research Report
  • [Presentation] マウス胚性腫瘍細胞P19の神経細胞への分化過程で発言変動する遺伝子の解析2009

    • Author(s)
      鈴木雅代、和田彩、実井綾、矢田知見、山内沙也香、小森理絵、小林隆信、喜納克仁、宮澤宏
    • Organizer
      日本薬学会第129年会
    • Place of Presentation
      京都
    • Related Report
      2009 Final Research Report
  • [Presentation] DNA損傷を認識するDNAアプタマーの探索2008

    • Author(s)
      森川雅行、喜納克仁、小森理絵、小林隆信、宮澤宏
    • Organizer
      日本薬学会第128年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2008-03-26
    • Related Report
      2007 Annual Research Report
  • [Presentation] Synthesis and reactivity of vitamin B2 derivatives photooxidizing guanine2008

    • Author(s)
      Kino K., Kobayashi T., Morikawa M., Komori R., Kobayashi T., Miyazawa H.
    • Organizer
      日本環境変異原学会第38回大会
    • Place of Presentation
      那覇
    • Related Report
      2009 Final Research Report
  • [Presentation] マウス胚性腫瘍細胞P19の神経分化過程における遺伝子発現解析2008

    • Author(s)
      小森理絵, 鈴木雅代, 和田彩, 徳田佳子, 中山智子, 小林隆信, 喜納克仁, 宮澤宏
    • Organizer
      日本分子生物学会第31回年会・日本生化学会第81回大会
    • Place of Presentation
      神戸
    • Related Report
      2009 Final Research Report
  • [Presentation] NF-κBとDNAの結合を阻害する新規低分子阻害剤の同定2008

    • Author(s)
      小林隆信、吉森篤史、喜納克仁、小森理絵、田沼靖一、宮澤宏
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Related Report
      2009 Final Research Report
  • [Presentation] グアニン酸化損傷の効果の予測2008

    • Author(s)
      喜納克仁
    • Organizer
      第11回生命化学研究会
    • Place of Presentation
      水上(群馬)
    • Related Report
      2009 Final Research Report
  • [Presentation] ビタミンB2からのフラビン誘導体の合成2008

    • Author(s)
      小林輝彦, 喜納克仁, 小林隆信, 小森理絵, 宮澤宏
    • Organizer
      第47回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会
    • Place of Presentation
      岡山
    • Related Report
      2009 Final Research Report
  • [Presentation] DNA酸化損傷に対して特異的に認識するDNAアプタマーの探索2008

    • Author(s)
      森川雅行, 喜納克仁, 千田岳史, 小林輝彦, 小森理絵, 小林隆信, 宮澤宏
    • Organizer
      第47回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会
    • Place of Presentation
      岡山
    • Related Report
      2009 Final Research Report
  • [Presentation] チアゾールオレンジのエキシトン相互作用を利用したDNAプローブの配列依存的な蛍光特性2008

    • Author(s)
      池田修司、久保田健、喜納克仁、岡本晃充
    • Organizer
      第3回バイオ関連化学合同シンポジウム
    • Related Report
      2009 Final Research Report
  • [Presentation] グアニン酸化損傷を認識するDNAアプタマーの探索2008

    • Author(s)
      喜納克仁、森川雅行、小林輝彦、小森理絵、小林隆信、宮澤宏
    • Organizer
      第3回バイオ関連化学合同シンポジウム
    • Related Report
      2009 Final Research Report
  • [Presentation] 核酸結合タンパク質EWSのDNA結合性の解析2008

    • Author(s)
      高濱謙太郎、喜納克仁、内山裕美子、大吉崇文
    • Organizer
      第3回バイオ関連化学合同シンポジウム
    • Related Report
      2009 Final Research Report
  • [Presentation] フラビン誘導体の合成2008

    • Author(s)
      喜納克仁
    • Organizer
      新世代の生物有機化学研究会
    • Related Report
      2009 Final Research Report
  • [Presentation] DNA損傷を認識するDNAアプタマーの探索2008

    • Author(s)
      森川雅行、喜納克仁、小林輝彦、小森理絵、小林隆信、宮澤宏
    • Organizer
      日本薬学会第128年会
    • Related Report
      2009 Final Research Report
  • [Presentation] Detection of base pairing of an oxidatively damaged guanine using colloidal stability change of DNA-linked polymer micelles.2007

    • Author(s)
      Takarada T, Imaizumi E, Kino K, Yoshimoto K, Ogasawara S, Kanayama N, Maeda M
    • Organizer
      5th International Symposium on Nucleic Acids Chemistry
    • Place of Presentation
      Tokyo, Japan
    • Related Report
      2009 Final Research Report
  • [Presentation] マウス胚性腫瘍細胞P19の神経分化にかかわる遺伝子・タンパク質の網羅的探索2007

    • Author(s)
      小森理絵、木内郁代、松田島英里、小林隆信、喜納克仁、宮澤宏
    • Organizer
      第30回日本分子生物学会年会第80回日本生化学会大会合同大会
    • Related Report
      2009 Final Research Report
  • [Presentation] グアニン酸化の新規生成経路2007

    • Author(s)
      喜納克仁
    • Organizer
      新世代の生物有機化学研究会2007
    • Related Report
      2009 Final Research Report
  • [Book] Prog. DNA Damage Res.(Molecular basis of guanine oxidation under UV-A/VIS radiation and its biological effects., Chapter X)2008

    • Author(s)
      Kino K. *, Sugiyama H, Miyazawa H.
    • Related Report
      2009 Final Research Report
  • [Book] 13章 遺伝子損傷の分子機構(化学フロンティア ゲノム化学の最前線-医学、分子生物学への応用と展開-)2007

    • Author(s)
      喜納克仁
    • Related Report
      2009 Final Research Report
  • [Remarks] Kino, K., Ikeda, S., Okamoto, A. NABiT SF 2. 0

    • URL

      http://kp.bunri-u.ac.jp/kph04/index.htm1

    • Related Report
      2009 Final Research Report
  • [Remarks] 喜納克仁、小林輝彦、有馬英治、小森理絵、小林隆信、宮澤宏「フラビン誘導体の光分解生物とグアニン光酸化反応」ビタミン,2010, 84, 59-60.

    • Related Report
      2009 Final Research Report
  • [Remarks] 喜納克仁、宮澤宏、杉山弘 「フラビン結合オリゴマーの簡易合成法と光反応解析」ビタミン, 2008, 82, 35-37.

    • Related Report
      2009 Final Research Report
  • [Remarks] 喜納克仁 (2009) 「グアニン酸化損傷の発生、変異、修復」12 月、京都大学

    • Related Report
      2009 Final Research Report
  • [Remarks] 喜納克仁 (2008)「グアニン酸化損傷の生体内への影響」北陸先端科学技術大学院大学セミナー 10 月、能美(石川)

    • Related Report
      2009 Final Research Report
  • [Remarks] 喜納克仁「DNA 化学反応 ~がん・老化のきっかけ~」第185 回 やさしい科学技術セミナー (主催:JapanPrize) 2008 年9 月13 日実施※youtube での公開http://www.youtube.com/watch?v=gI6CZ33oIkE

    • Related Report
      2009 Final Research Report
  • [Remarks] 喜納克仁「最新のDNA 研究理解」四国新聞, 2008, 9 月14 日, 22.

    • Related Report
      2009 Final Research Report
  • [Remarks]

    • URL

      http://kp.bunri-u.ac.jp/kph04/index.html

    • Related Report
      2008 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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