Isolation and analysis of UV-resistant mutants using FOX hunting syste

• Project/Area Number: 19710055
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: KONDOU Youichi The Institute of Physical and Chemical Research, 植物ゲノム機能研究チーム, 特別研究員 (00391954)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,740,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥540,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,400,000 (Direct Cost: ¥1,400,000)
• Keywords: 紫外線耐性 / 紫外線応答 / 二次代謝産物 / 紫外線 / 環境応答 / 植物 / 過剰発現 / 有用遺伝子

Research Abstract

We isolated a mutant showing UV-resistance from Arabidopsis FOX lines by using novel method for screening such mutants. UV resistance of the mutant was caused by more accumulation of UV-absorbing compounds. Interestingly, levels of transcription of genes coding synthesis enzymes for secondary metabolite were increased in the mutant. These results suggest that the gene, which causes UV resistance to Arabidopsis, regulate defense response against UV irradiation.

Research Products

• [Journal Article] High-throughput characterization of plant gene functions by using gain-of-function technology (2010)
  • Author(s): Kondou Youichi
  • Journal Title: Annual review of Plant Biology (in press)
• [Journal Article] Application of full-length cDNA resources to gain-of-function technology for characterization of plant gene function (2010)
  • Author(s): Kondou Youichi
  • Journal Title: Methods in Molecular Biology (in press)
• [Journal Article] Full-length cDNA overexpressor gene hunting system(FOX hunting system) (2010)
  • Author(s): Higuchi Mieko
  • Journal Title: Plant Reverse Genetics (in press)
• [Journal Article] Phenome Analysis in Plant Species Using Loss-of-Function and Gain-of-Function Mutants. (2009)
  • Author(s): Kuromori T, Takahashi S, Kondou Y, Shinozaki K, Matsui M.
  • Journal Title: Plant Cell Physiology 50(7) Pages: 1215-1231
  • NAID: 10027341294
  • Peer Reviewed
• [Journal Article] Phenome Analysis in Plant Species Using Loss-of-Function and Gain-of-Function (2009)
  • Author(s): Kuromori Takashi
  • Journal Title: Plant Cell Physiology 50 Pages: 1215-1231
  • Peer Reviewed
• [Journal Article] RETARDED GROWTH OF EMBRYO1, a new basic helix-loop-helix protein, expresses in endosperm to control embryo growth. (2008)
  • Author(s): Kondou Y, Nakazawa M, Kawashima M, Ichikawa T, Yoshizumi T, Suzuki K, Ishikawa A, Koshi T, Matsui R, Muto S, Matsui M.
  • Journal Title: Plant Physiology 147(4) Pages: 1924-35
  • Peer Reviewed
• [Journal Article] RETRADED GROWTH OF EMBRYOl, a new basic helix-loop-helix protein, expresses in endosperm to control embryo growth (2008)
  • Author(s): Kondou Youichi
  • Journal Title: Plant Physiology 147(4) Pages: 1924-1935
  • Peer Reviewed
• [Journal Article] High-throughput characterization of plant gene functions by using gain-of-function technology.
  • Author(s): Kondou Y, Higuchi M, Matsui M.
  • Journal Title: Annual review of Plant Biology (in press)
• [Journal Article] Application of full-length cDNA resources to gain-of-function technology for characterization of plant gene function.
  • Author(s): Kondou Y, Higuchi M, Matsui M.
  • Journal Title: Methods in Molecular Biology (in press)
• [Journal Article] Full-length cDNA overexpressor gene hunting system (FOX hunting system).
  • Author(s): Higuchi M, Kondou Y, Matsui M.
  • Journal Title: Plant Reverse Genetics (in press)
• [Presentation] 機能誘導系を利用した新規転写因子過剰発現系統より単離した光応答に異常を示す変異体の解析 (2010)
  • Author(s): 近藤陽一
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 鹿児島
  • Year and Date: 2010-03-20
• [Presentation] Establishments of novel gain-of-function resource for functional analysis of transcription factors and analysis of novel transcription factor related with hypocotyl growth under light condition. (2009)
  • Author(s): 近藤陽一
  • Organizer: The 20th International Conference on Arabidopsis Research
  • Place of Presentation: エジンバラ(イギリス)
  • Year and Date: 2009-07-02
• [Presentation] Establishments of novel gain-of-function resource for functional analysis of transcription factors and analysis of novel transcription factor related with hypocotyl growth under light condition (2009)
  • Author(s): 近藤陽一
  • Organizer: The 20th International Conference on Arabidopsis Research
  • Place of Presentation: エジンバラ(UK)
  • Year and Date: 2009-07-02
• [Presentation] 機能誘導系を利用した新規転写因子過剰発現系統の作出と光低感受性変異体の単離と解析 (2009)
  • Author(s): 近藤陽一
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-03-21
• [Presentation] イネFOXラインからの色素高蓄積変異体の単離と解析 (2007)
  • Author(s): 近藤陽一
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
  • Place of Presentation: 横浜. 近藤
  • Year and Date: 2007-12-12
• [Presentation] イネFOXラインからの色素高蓄積変異体の単離と解析 (2007)
  • Author(s): 近藤陽一、川島美香、市川尚斉、長谷川由香子、石川明苗、蒔田由布子、廣近洋彦、松井南
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-12
• [Presentation] Isolation of rice cDNAs for higher accumulation of pigments from rice FOX lines (2007)
  • Author(s): 近藤陽一
  • Organizer: The 18th International Conference on Arabidopsis Research
  • Place of Presentation: 北京(中国)
  • Year and Date: 2007-06-21
• [Presentation] Isolation of rice cDNAs for higher accumulation of pigments from rice FOX lines (2007)
  • Author(s): Youichi Kondou, Mika Kawashima, Takanari Ichikawa, Akie Ishikawa, Yukako Hasegawa, Yuko Makita Hirohiko Hirochika and Minami Matsui
  • Organizer: The 18th lnternational Conference on Arabidop sis Research
  • Place of Presentation: 北京
  • Year and Date: 2007-06-21