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Quantitative analysis on mutation induced by DNA adducts derived from diesel emission using human cells

Research Project

Project/Area Number 19710058
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Risk sciences of radiation/Chemicals
Research InstitutionOsaka Prefecture University

Principal Investigator

KAWANISHI Masanobu  Osaka Prefecture University, 産学官連携機構, 助教 (70332963)

Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥3,630,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywords環境質定量化 / 核酸 / 遺伝子 / TLS / DNA複製 / 大気汚染物質 / DNA付加体 / DNA損傷 / 突然変異
Research Abstract

アミノビフェニル(ABP)に由来するDNA付加体を部位特異的に1箇所もつプラスミドを作製し、ヒト細胞内で複製させた。複製プラスミドを解析し、損傷乗り越えDNA合成(TLS)の頻度と突然変異パターンを明らかにした。その結果、付加体周辺の塩基配列がTLS頻度と変異誘率に影響を与えることがわかった。またABP付加体をTLSする際に働くポリメラーゼの種類も配列によって異なることが分かった。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (32 results)

All 2009 2008 2007

All Journal Article (14 results) (of which Peer Reviewed: 14 results) Presentation (17 results) Book (1 results)

  • [Journal Article] Genotoxicity of 3,6-dinitrobenzo[e]pyrene, a novel mutagen in ambient air and surface soil, in mammalian cells in vitro and in vivo2009

    • Author(s)
      Tetsushi Watanabe, Soichiro Hagio, Sayaka Ogo, Chiaki Shimohara, Rika Jouchi, Saori Takayama, Tomohiro Hasei, Teruhisa Hirayama, Yoshimitsu Oda, Takashi Yagi
    • Journal Title

      Mutagenesis 24(3)

      Pages: 279-284

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mutagenic specificity of N-nitrosotaurocholic acid in supF shuttle vector plasmids.2009

    • Author(s)
      Masanobu Kawanishi, Hiroshi Nishida, Yukari Totsuka, Koichi Nishimura, Keiji Wakabayashi, Takashi Yagi
    • Journal Title

      Genes and Environment 31(1)

      Pages: 9-14

    • NAID

      110007138018

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Establishment of a method for analyzing translesion DNA synthesis across a single bulky adduct in human cells.2009

    • Author(s)
      Tomoko Sawai, Masanobu Kawanishi, Takeji Takamura-Enya, Takashi Yagi
    • Journal Title

      Genes and Environment 31(1)

      Pages: 24-30

    • NAID

      110007138016

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Validation of a new yeast-based reporter assay consisting of human estrogen receptors alpha/beta and coactivator SRC-1: Application for detection of estrogenic activity in environmental samples.2009

    • Author(s)
      Wai-Ling Chu, Kazuhiro Shiizaki, Masanobu Kawanishi, Takashi Yagi
    • Journal Title

      Environmental Toxicology (in press)

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Establishment of a Method for Analyzing Translesion DNA Synthesis across a Single Bulky Adduct in Human Cells2009

    • Author(s)
      T. Sawai, M. Kawanishi, et.al.
    • Journal Title

      Genes and Environment 31

      Pages: 24-30

    • NAID

      110007138016

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Omeprazole alleviates Benzo[a]pyrene cytotoxicity by inhibition of CYP1A1 activity in human and mouse hepatoma cells2008

    • Author(s)
      Kazuhiro Shiizaki, Seiichiroh Ohsako. Masanobu Kawanishi, Takashi Yagi
    • Journal Title

      Basic and Clinical Pharmacology and Toxicology 103(5)

      Pages: 468-475

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Construction of a reporter yeast strain to detect estrogen receptor signaling through aryl hydrocarbon receptor activation.2008

    • Author(s)
      Masanobu Kawanishi, Mami Kondo, Kazuhiro Shiizaki, Wai Ling Chu, Yuichi Terasoma, Takashi Yagi
    • Journal Title

      Environmental Science and Technology 42(18)

      Pages: 468-475

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mutagenic specificity of N-acetoxy-3-aminobenzanthrone, a major metabolically activated form of 3-nitrobenzanthrone, in shuttle vector plasmids propagated in human cells2008

    • Author(s)
      Hiroshi Nishida, Masanobu Kawanishi, Takeji Takamura, Takashi Yagi
    • Journal Title

      Mutation Research 654(1)

      Pages: 82-87

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mutagenic specificity of N-acetoxy-3-aminobenzanthrone, a major metabolically activated form of 3-nitrobenzanthrone, in shuttle vector plasmids propagated in human cells2008

    • Author(s)
      H. Nishida, M. Kawanishi, et.al.
    • Journal Title

      Mutation Research 654

      Pages: 82-87

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural identification of DNA adducts derived from 3-nitrobenzanthrone, a potent carcinogen present in the atmosphere.2007

    • Author(s)
      Takeji Takamura-Enya, Masanobu Kawanishi, Takashi Yagi, Yoshiharu Hisamatsu
    • Journal Title

      Chemistry An Asian Journal 2(9)

      Pages: 1174-1185

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] DNA adduct formation in human hepatoma cells treated with 3-nitrobenzanthrone: analysis by the 32P-postlabeling method2007

    • Author(s)
      Takaharu Kanno, Masanobu Kawanishi, Takeji Takamura-Enya, Volker M. Arlt, David H. Phillips, Takashi Yagi
    • Journal Title

      Mutation Research 634(1-2)

      Pages: 184-191

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular Evidence of Involvement of Nucleotide Excision Repair(NER)System in Repair of the Mono ADP-ribosylated DNA Adduct Produced by Pierisin-1,an Apoptosis-inducing Protein from Cabbage Butterfly2007

    • Author(s)
      Masanobu Kawanishi, et. al.
    • Journal Title

      Chemical Research in Toxicology 20

      Pages: 694-700

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] DNA adduct formation in human hepatoma cells treated with 3-nitrobenzanthrone:analysis by the 32P-postlabeling method2007

    • Author(s)
      Takaharu Kanno, Masanobu Kawanishi, et. al.
    • Journal Title

      Mutation Research 634

      Pages: 184-191

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural Identification of DNA Adducts Derived from 3-Nitrobeiizaiithrone,a Potent Carcinogen Present in the Atmosphere2007

    • Author(s)
      Takeji Takamura-Enya, Masanobu Kawanishi, et. al.
    • Journal Title

      Chemistry An Asian Journal 2

      Pages: 1174-1185

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 大気汚染物質3-ニトロベンズアントロンによるDNA付加体の生成とTLS・突然変異2008

    • Author(s)
      川西優喜, 西田裕, 石井宏, 菅野毅治, Robert Fuchs, 松田知成, 高村岳樹, 八木孝司
    • Organizer
      日本分子生物学会第31回大会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-12
    • Related Report
      2008 Final Research Report
  • [Presentation] Agonistic and Antagonistic Activities of various Chemicals in Reporter Yeasts Expressing Estrogen Receptors α and β2008

    • Author(s)
      Wai-Ling Chu, Kazuhiro Shiizaki, Masanobu Kawanishi, Takashi Yagi
    • Organizer
      日本環境変異原学会第37回大会
    • Place of Presentation
      沖縄
    • Year and Date
      2008-12-04
    • Related Report
      2008 Final Research Report
  • [Presentation] Significance of DNA Adductome Analysis in In Vitro Microcucleus Test2008

    • Author(s)
      Kyoko Kato, Eiji Yamamura, Masanobu Kawanishi,Takashi Yagi, Tomonari Matsuda, Akio Sugiyama, Yoshifumi Uno
    • Organizer
      本環境変異原学会第37回大会
    • Place of Presentation
      沖縄
    • Year and Date
      2008-12-04
    • Related Report
      2008 Final Research Report
  • [Presentation] Efficiency and Fidelity of Translesion DNA Synthesis across 3-nitrobenzanthrone-DNA-adducts2008

    • Author(s)
      Hiroshi Nishida, Masanobu Kawanishi, Takeji Takamura, Takashi Yagi
    • Organizer
      日本環境変異原学会第37回大会
    • Place of Presentation
      沖縄
    • Year and Date
      2008-12-04
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] Quantitative Analysis of Translesion DNA Synthesis across Site-specific 4-Aminobiphenyl Adducts in Human Cells2008

    • Author(s)
      Tomoko Sawai, Mawanobu Kawanishi, Takaharu Kanno, Takeji Takamura-Enta, Takashi Yagi
    • Organizer
      日本環境変異原学会第37回大会
    • Place of Presentation
      沖縄
    • Year and Date
      2008-12-04
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] Evaluation of Endocrine Disruptors Using Reporter Yeasts Expressing Human Androgen Receptor and Thyroid Hormone Receptor2008

    • Author(s)
      Shingo Ebata, Shota ASAI, Kazuhiro Shiizaki, Masanobu Kawanishi, Takashi Yagi
    • Organizer
      日本環境変異原学会第37回大会
    • Place of Presentation
      沖縄
    • Year and Date
      2008-12-04
    • Related Report
      2008 Final Research Report
  • [Presentation] Evaluation of Genotoxicity of 3,6-Dinitrobenzo[e]pyrene in Human Cell Line2008

    • Author(s)
      Soichiro Hagio, Sayaka Ogo, Masanobu Kawanishi, Tetsushi Watanabe, Yoshimitsu Oda, Takashi Yagi
    • Organizer
      日本環境変異原学会第37回大会
    • Place of Presentation
      沖縄
    • Year and Date
      2008-12-04
    • Related Report
      2008 Final Research Report
  • [Presentation] TCDD Represses Benzo[a]pyrene-DNA Adduct Formation in HepG2 cell2008

    • Author(s)
      cell, Kazuhiro Shiizaki, Masanobu Kawanishi, Takashi Yagi
    • Organizer
      日本環境変異原学会第37回大会
    • Place of Presentation
      沖縄
    • Year and Date
      2008-12-04
    • Related Report
      2008 Final Research Report
  • [Presentation] Genotoxicity of Nanomaterials2008

    • Author(s)
      Sayaka Ogo, Masanobu Kawanishi, Yukari Totsuka, Keiji Wakabayashi, Takashi Yagi
    • Organizer
      日本環境変異原学会第37回大会
    • Place of Presentation
      沖縄
    • Year and Date
      2008-12-04
    • Related Report
      2008 Final Research Report
  • [Presentation] Evaluation of Genotoxicity of 3, 6-Dinitrobenzo[e]pyrene in Human Cell Lines2008

    • Author(s)
      S. Hagio, S. Ogo, M. Kawanishi, et.al.
    • Organizer
      日本環境変異原学会第37回大会
    • Place of Presentation
      沖縄
    • Year and Date
      2008-12-04
    • Related Report
      2008 Annual Research Report
  • [Presentation] Mutaions produced by N-nitrosotaurocholic acid in the supF target gene replicated in human cells2007

    • Author(s)
      Hiroshi NISHIDA, Masanobu KAWANISHI, Yukari TOTSUKA, Keiji WAKABAYASHI, Takashi YAGI
    • Organizer
      1st Asian/36th Japanese conference on environmental mutagens
    • Place of Presentation
      Kokura, Japan
    • Year and Date
      2007-11-29
    • Related Report
      2008 Final Research Report
  • [Presentation] Consequence of Translesion DNA Synthesis across Site-specific 4-Aminobiphenyl Adducts in Human Cells2007

    • Author(s)
      Tomoko SAWAI, Masanobu KAWANISHI, Takaharu KANNO, Takeji TAKAMURA-ENYA, Takashi YAGI
    • Organizer
      1st Asian/36th Japanese conference on environmental mutagens
    • Place of Presentation
      Kokura, Japan
    • Year and Date
      2007-11-29
    • Related Report
      2008 Final Research Report
  • [Presentation] Establishment of reporter assay yeast expressing intact human nuclear receptor2007

    • Author(s)
      Kazuhiro SHIIZAKI, Shouta ASAI, Shingo EBATA, Mabel CHU, Takashi YAGI, Masanobu KAWANISHI
    • Organizer
      1st Asian/36th Japanese conference on environmental mutagens
    • Place of Presentation
      Kokura, Japan
    • Year and Date
      2007-11-29
    • Related Report
      2008 Final Research Report
  • [Presentation] Construction of Reporter Yeast for Estrogen Receptor Signalling Activity through Aryl hydrocarbon Receptor Activation2007

    • Author(s)
      Masanobu Kawanishi, Mami Kondo, Kazuhiro Shiizaki Yuichi Terasoma, Mabel Chu and Takashi Yagi
    • Organizer
      1st Asian/36th Japanese conference on environmental mutagens
    • Place of Presentation
      Kokura, Japan
    • Year and Date
      2007-11-29
    • Related Report
      2008 Final Research Report
  • [Presentation] Mutaions produced by N-nitrosotaurocholic acid in the supF target genereplicated in human cells2007

    • Author(s)
      Hiroshi NISHIDA, Masanobu KAWANISHI, et. al.
    • Organizer
      1st Asian/36 th Japanese conference on environmental mutagens
    • Place of Presentation
      小倉市
    • Year and Date
      2007-11-29
    • Related Report
      2007 Annual Research Report
  • [Presentation] Consequence of Translesion DNA Synthesis across Site-specific 4-Aminobiphenyl Adducts in Human Cells2007

    • Author(s)
      Tomoko SAWAI, Masanobu KAWANISHI, et. al.
    • Organizer
      1st Asian/36 th Japanese conference on environmental mutagens
    • Place of Presentation
      小倉市
    • Year and Date
      2007-11-29
    • Related Report
      2007 Annual Research Report
  • [Presentation] バイリング(Pleurotus nebrodensis)由来のlaccaseによるエストリオールの除去2007

    • Author(s)
      新谷香代, 上田光宏, 仲西亜希子, 中澤昌美, 辻山彰一, 川西優喜, 八木孝司, 宮武和孝
    • Organizer
      日本きのこ学会第11回大会
    • Place of Presentation
      旭川市
    • Year and Date
      2007-09-18
    • Related Report
      2008 Final Research Report
  • [Book] みんなのくらしと放射線2008

    • Author(s)
      分担執筆74-77、「みんなのくらしと放射線」知識普及実行委員会編
    • Total Pages
      202
    • Publisher
      大阪公立大学共同出版会
    • Related Report
      2008 Final Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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