Quantitative analysis on mutation induced by DNA adducts derived from diesel emission using human cells
| Project/Area Number |
19710058
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
KAWANISHI Masanobu Osaka Prefecture University, 産学官連携機構, 助教 (70332963)
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| Project Period (FY) |
2007 – 2008
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| Project Status |
Completed (Fiscal Year 2008)
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| Budget Amount *help |
¥3,630,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | 環境質定量化 / 核酸 / 遺伝子 / TLS / DNA複製 / 大気汚染物質 / DNA付加体 / DNA損傷 / 突然変異 |
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| Research Abstract |
アミノビフェニル(ABP)に由来するDNA付加体を部位特異的に1箇所もつプラスミドを作製し、ヒト細胞内で複製させた。複製プラスミドを解析し、損傷乗り越えDNA合成(TLS)の頻度と突然変異パターンを明らかにした。その結果、付加体周辺の塩基配列がTLS頻度と変異誘率に影響を与えることがわかった。またABP付加体をTLSする際に働くポリメラーゼの種類も配列によって異なることが分かった。
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Report
(3 results)
Research Products
(32 results)
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[Journal Article] Genotoxicity of 3,6-dinitrobenzo[e]pyrene, a novel mutagen in ambient air and surface soil, in mammalian cells in vitro and in vivo2009
Author(s)
Tetsushi Watanabe, Soichiro Hagio, Sayaka Ogo, Chiaki Shimohara, Rika Jouchi, Saori Takayama, Tomohiro Hasei, Teruhisa Hirayama, Yoshimitsu Oda, Takashi Yagi
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Journal Title
Mutagenesis 24(3)
Pages: 279-284
Related Report
Peer Reviewed
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