Quantitative analysis on mutation induced by DNA adducts derived from diesel emission using human cells

• Project/Area Number: 19710058
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Osaka Prefecture University
• Principal Investigator: KAWANISHI Masanobu Osaka Prefecture University, 産学官連携機構, 助教 (70332963)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,630,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥2,200,000 (Direct Cost: ¥2,200,000)
• Keywords: 環境質定量化 / 核酸 / 遺伝子 / TLS / DNA複製 / 大気汚染物質 / DNA付加体 / DNA損傷 / 突然変異

Research Abstract

アミノビフェニル(ABP)に由来するDNA付加体を部位特異的に1箇所もつプラスミドを作製し、ヒト細胞内で複製させた。複製プラスミドを解析し、損傷乗り越えDNA合成(TLS)の頻度と突然変異パターンを明らかにした。その結果、付加体周辺の塩基配列がTLS頻度と変異誘率に影響を与えることがわかった。またABP付加体をTLSする際に働くポリメラーゼの種類も配列によって異なることが分かった。

Research Products

• [Journal Article] Genotoxicity of 3,6-dinitrobenzo[e]pyrene, a novel mutagen in ambient air and surface soil, in mammalian cells in vitro and in vivo (2009)
  • Author(s): Tetsushi Watanabe, Soichiro Hagio, Sayaka Ogo, Chiaki Shimohara, Rika Jouchi, Saori Takayama, Tomohiro Hasei, Teruhisa Hirayama, Yoshimitsu Oda, Takashi Yagi
  • Journal Title: Mutagenesis 24(3) Pages: 279-284
  • Peer Reviewed
• [Journal Article] Mutagenic specificity of N-nitrosotaurocholic acid in supF shuttle vector plasmids. (2009)
  • Author(s): Masanobu Kawanishi, Hiroshi Nishida, Yukari Totsuka, Koichi Nishimura, Keiji Wakabayashi, Takashi Yagi
  • Journal Title: Genes and Environment 31(1) Pages: 9-14
  • NAID: 110007138018
  • Peer Reviewed
• [Journal Article] Establishment of a method for analyzing translesion DNA synthesis across a single bulky adduct in human cells. (2009)
  • Author(s): Tomoko Sawai, Masanobu Kawanishi, Takeji Takamura-Enya, Takashi Yagi
  • Journal Title: Genes and Environment 31(1) Pages: 24-30
  • NAID: 110007138016
  • Peer Reviewed
• [Journal Article] Validation of a new yeast-based reporter assay consisting of human estrogen receptors alpha/beta and coactivator SRC-1: Application for detection of estrogenic activity in environmental samples. (2009)
  • Author(s): Wai-Ling Chu, Kazuhiro Shiizaki, Masanobu Kawanishi, Takashi Yagi
  • Journal Title: Environmental Toxicology (in press)
  • Peer Reviewed
• [Journal Article] Establishment of a Method for Analyzing Translesion DNA Synthesis across a Single Bulky Adduct in Human Cells (2009)
  • Author(s): T. Sawai, M. Kawanishi, et.al.
  • Journal Title: Genes and Environment 31 Pages: 24-30
  • NAID: 110007138016
  • Peer Reviewed
• [Journal Article] Omeprazole alleviates Benzo[a]pyrene cytotoxicity by inhibition of CYP1A1 activity in human and mouse hepatoma cells (2008)
  • Author(s): Kazuhiro Shiizaki, Seiichiroh Ohsako. Masanobu Kawanishi, Takashi Yagi
  • Journal Title: Basic and Clinical Pharmacology and Toxicology 103(5) Pages: 468-475
  • Peer Reviewed
• [Journal Article] Construction of a reporter yeast strain to detect estrogen receptor signaling through aryl hydrocarbon receptor activation. (2008)
  • Author(s): Masanobu Kawanishi, Mami Kondo, Kazuhiro Shiizaki, Wai Ling Chu, Yuichi Terasoma, Takashi Yagi
  • Journal Title: Environmental Science and Technology 42(18) Pages: 468-475
  • Peer Reviewed
• [Journal Article] Mutagenic specificity of N-acetoxy-3-aminobenzanthrone, a major metabolically activated form of 3-nitrobenzanthrone, in shuttle vector plasmids propagated in human cells (2008)
  • Author(s): Hiroshi Nishida, Masanobu Kawanishi, Takeji Takamura, Takashi Yagi
  • Journal Title: Mutation Research 654(1) Pages: 82-87
  • Peer Reviewed
• [Journal Article] Mutagenic specificity of N-acetoxy-3-aminobenzanthrone, a major metabolically activated form of 3-nitrobenzanthrone, in shuttle vector plasmids propagated in human cells (2008)
  • Author(s): H. Nishida, M. Kawanishi, et.al.
  • Journal Title: Mutation Research 654 Pages: 82-87
  • Peer Reviewed
• [Journal Article] Structural identification of DNA adducts derived from 3-nitrobenzanthrone, a potent carcinogen present in the atmosphere. (2007)
  • Author(s): Takeji Takamura-Enya, Masanobu Kawanishi, Takashi Yagi, Yoshiharu Hisamatsu
  • Journal Title: Chemistry An Asian Journal 2(9) Pages: 1174-1185
  • Peer Reviewed
• [Journal Article] DNA adduct formation in human hepatoma cells treated with 3-nitrobenzanthrone: analysis by the 32P-postlabeling method (2007)
  • Author(s): Takaharu Kanno, Masanobu Kawanishi, Takeji Takamura-Enya, Volker M. Arlt, David H. Phillips, Takashi Yagi
  • Journal Title: Mutation Research 634(1-2) Pages: 184-191
  • Peer Reviewed
• [Journal Article] Molecular Evidence of Involvement of Nucleotide Excision Repair(NER)System in Repair of the Mono ADP-ribosylated DNA Adduct Produced by Pierisin-1,an Apoptosis-inducing Protein from Cabbage Butterfly (2007)
  • Author(s): Masanobu Kawanishi, et. al.
  • Journal Title: Chemical Research in Toxicology 20 Pages: 694-700
  • Peer Reviewed
• [Journal Article] DNA adduct formation in human hepatoma cells treated with 3-nitrobenzanthrone:analysis by the 32P-postlabeling method (2007)
  • Author(s): Takaharu Kanno, Masanobu Kawanishi, et. al.
  • Journal Title: Mutation Research 634 Pages: 184-191
  • Peer Reviewed
• [Journal Article] Structural Identification of DNA Adducts Derived from 3-Nitrobeiizaiithrone,a Potent Carcinogen Present in the Atmosphere (2007)
  • Author(s): Takeji Takamura-Enya, Masanobu Kawanishi, et. al.
  • Journal Title: Chemistry An Asian Journal 2 Pages: 1174-1185
  • Peer Reviewed
• [Presentation] 大気汚染物質3-ニトロベンズアントロンによるDNA付加体の生成とTLS・突然変異 (2008)
  • Author(s): 川西優喜, 西田裕, 石井宏, 菅野毅治, Robert Fuchs, 松田知成, 高村岳樹, 八木孝司
  • Organizer: 日本分子生物学会第31回大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] Agonistic and Antagonistic Activities of various Chemicals in Reporter Yeasts Expressing Estrogen Receptors α and β (2008)
  • Author(s): Wai-Ling Chu, Kazuhiro Shiizaki, Masanobu Kawanishi, Takashi Yagi
  • Organizer: 日本環境変異原学会第37回大会
  • Place of Presentation: 沖縄
  • Year and Date: 2008-12-04
• [Presentation] Significance of DNA Adductome Analysis in In Vitro Microcucleus Test (2008)
  • Author(s): Kyoko Kato, Eiji Yamamura, Masanobu Kawanishi,Takashi Yagi, Tomonari Matsuda, Akio Sugiyama, Yoshifumi Uno
  • Organizer: 本環境変異原学会第37回大会
  • Place of Presentation: 沖縄
  • Year and Date: 2008-12-04
• [Presentation] Efficiency and Fidelity of Translesion DNA Synthesis across 3-nitrobenzanthrone-DNA-adducts (2008)
  • Author(s): Hiroshi Nishida, Masanobu Kawanishi, Takeji Takamura, Takashi Yagi
  • Organizer: 日本環境変異原学会第37回大会
  • Place of Presentation: 沖縄
  • Year and Date: 2008-12-04
• [Presentation] Quantitative Analysis of Translesion DNA Synthesis across Site-specific 4-Aminobiphenyl Adducts in Human Cells (2008)
  • Author(s): Tomoko Sawai, Mawanobu Kawanishi, Takaharu Kanno, Takeji Takamura-Enta, Takashi Yagi
  • Organizer: 日本環境変異原学会第37回大会
  • Place of Presentation: 沖縄
  • Year and Date: 2008-12-04
• [Presentation] Evaluation of Endocrine Disruptors Using Reporter Yeasts Expressing Human Androgen Receptor and Thyroid Hormone Receptor (2008)
  • Author(s): Shingo Ebata, Shota ASAI, Kazuhiro Shiizaki, Masanobu Kawanishi, Takashi Yagi
  • Organizer: 日本環境変異原学会第37回大会
  • Place of Presentation: 沖縄
  • Year and Date: 2008-12-04
• [Presentation] Evaluation of Genotoxicity of 3,6-Dinitrobenzo[e]pyrene in Human Cell Line (2008)
  • Author(s): Soichiro Hagio, Sayaka Ogo, Masanobu Kawanishi, Tetsushi Watanabe, Yoshimitsu Oda, Takashi Yagi
  • Organizer: 日本環境変異原学会第37回大会
  • Place of Presentation: 沖縄
  • Year and Date: 2008-12-04
• [Presentation] TCDD Represses Benzo[a]pyrene-DNA Adduct Formation in HepG2 cell (2008)
  • Author(s): cell, Kazuhiro Shiizaki, Masanobu Kawanishi, Takashi Yagi
  • Organizer: 日本環境変異原学会第37回大会
  • Place of Presentation: 沖縄
  • Year and Date: 2008-12-04
• [Presentation] Genotoxicity of Nanomaterials (2008)
  • Author(s): Sayaka Ogo, Masanobu Kawanishi, Yukari Totsuka, Keiji Wakabayashi, Takashi Yagi
  • Organizer: 日本環境変異原学会第37回大会
  • Place of Presentation: 沖縄
  • Year and Date: 2008-12-04
• [Presentation] Evaluation of Genotoxicity of 3, 6-Dinitrobenzo[e]pyrene in Human Cell Lines (2008)
  • Author(s): S. Hagio, S. Ogo, M. Kawanishi, et.al.
  • Organizer: 日本環境変異原学会第37回大会
  • Place of Presentation: 沖縄
  • Year and Date: 2008-12-04
• [Presentation] Mutaions produced by N-nitrosotaurocholic acid in the supF target gene replicated in human cells (2007)
  • Author(s): Hiroshi NISHIDA, Masanobu KAWANISHI, Yukari TOTSUKA, Keiji WAKABAYASHI, Takashi YAGI
  • Organizer: 1st Asian/36th Japanese conference on environmental mutagens
  • Place of Presentation: Kokura, Japan
  • Year and Date: 2007-11-29
• [Presentation] Consequence of Translesion DNA Synthesis across Site-specific 4-Aminobiphenyl Adducts in Human Cells (2007)
  • Author(s): Tomoko SAWAI, Masanobu KAWANISHI, Takaharu KANNO, Takeji TAKAMURA-ENYA, Takashi YAGI
  • Organizer: 1st Asian/36th Japanese conference on environmental mutagens
  • Place of Presentation: Kokura, Japan
  • Year and Date: 2007-11-29
• [Presentation] Establishment of reporter assay yeast expressing intact human nuclear receptor (2007)
  • Author(s): Kazuhiro SHIIZAKI, Shouta ASAI, Shingo EBATA, Mabel CHU, Takashi YAGI, Masanobu KAWANISHI
  • Organizer: 1st Asian/36th Japanese conference on environmental mutagens
  • Place of Presentation: Kokura, Japan
  • Year and Date: 2007-11-29
• [Presentation] Construction of Reporter Yeast for Estrogen Receptor Signalling Activity through Aryl hydrocarbon Receptor Activation (2007)
  • Author(s): Masanobu Kawanishi, Mami Kondo, Kazuhiro Shiizaki Yuichi Terasoma, Mabel Chu and Takashi Yagi
  • Organizer: 1st Asian/36th Japanese conference on environmental mutagens
  • Place of Presentation: Kokura, Japan
  • Year and Date: 2007-11-29
• [Presentation] Mutaions produced by N-nitrosotaurocholic acid in the supF target genereplicated in human cells (2007)
  • Author(s): Hiroshi NISHIDA, Masanobu KAWANISHI, et. al.
  • Organizer: 1st Asian/36 th Japanese conference on environmental mutagens
  • Place of Presentation: 小倉市
  • Year and Date: 2007-11-29
• [Presentation] Consequence of Translesion DNA Synthesis across Site-specific 4-Aminobiphenyl Adducts in Human Cells (2007)
  • Author(s): Tomoko SAWAI, Masanobu KAWANISHI, et. al.
  • Organizer: 1st Asian/36 th Japanese conference on environmental mutagens
  • Place of Presentation: 小倉市
  • Year and Date: 2007-11-29
• [Presentation] バイリング(Pleurotus nebrodensis)由来のlaccaseによるエストリオールの除去 (2007)
  • Author(s): 新谷香代, 上田光宏, 仲西亜希子, 中澤昌美, 辻山彰一, 川西優喜, 八木孝司, 宮武和孝
  • Organizer: 日本きのこ学会第11回大会
  • Place of Presentation: 旭川市
  • Year and Date: 2007-09-18
• [Book] みんなのくらしと放射線 (2008)
  • Author(s): 分担執筆74-77、「みんなのくらしと放射線」知識普及実行委員会編
  • Total Pages: 202
  • Publisher: 大阪公立大学共同出版会