Development of structural analysis method for protein immobilized on nano-biodevice
| Project/Area Number |
19710096
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Nanomaterials/Nanobioscience
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| Research Institution | Shimane University |
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Principal Investigator |
AOYAGOI Satoka Shimane University, 生物資源科学部, 准教授 (20339683)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥3,490,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2007: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | タンパク質 / 分子認識 / 表面・界面物性 / ナノバイオ / 表面・界面物件 / タンバク質 |
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| Research Abstract |
Immobilized-protein samples such as cytochrome b5 and lysozyme were investigated using TOF-SIMS with different primary ion sources for evaluating their orientations. Since the suggested surface side of each protein was consistent with the immobilization conditions, it is suggested that TOF-SIMS has the ability to evaluate orientation of immobilized protein molecules. Moreover, it is also indicated that the larger cluster ion source C60+ provides superior information to determine the surface part of the immobilized protein.
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Report
(4 results)
Research Products
(22 results)
