| Budget Amount *help |
¥3,490,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2007: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Research Abstract |
Immobilized-protein samples such as cytochrome b5 and lysozyme were investigated using TOF-SIMS with different primary ion sources for evaluating their orientations. Since the suggested surface side of each protein was consistent with the immobilization conditions, it is suggested that TOF-SIMS has the ability to evaluate orientation of immobilized protein molecules. Moreover, it is also indicated that the larger cluster ion source C60+ provides superior information to determine the surface part of the immobilized protein.
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