| Budget Amount *help |
¥3,690,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Research Abstract |
We have demonstrated a methodology that realizes the site-selective anatomy of molecular interactions at the single-molecule level. With the combination of cross-linkers and the atomic force microscope that we developed to enable a precise analysis by dynamic force spectroscopy, direct and bridged interactions at each reaction site in a typical ligand-receptor system, sreptavidin-biotin complex, were clearly distinguished and individually analyzed for the first time, providing a greater understanding of step-by-step progress of the bonding process.
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