Molecular sorting system using kinesin and dynein motor protein

• Project/Area Number: 19710111
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Nanomaterials/Nanobioscience
• Research Institution: Ritsumeikan University
• Principal Investigator: YOKOKAWA Ryuji Ritsumeikan University, 理工学部, 講師 (10411216)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,780,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥1,700,000 (Direct Cost: ¥1,700,000)
• Keywords: ナノソーティング / キネシン / ダイニン / MicroTAS / MEMS

Research Abstract

モータタンパク質であるキネシンとダイニンの微小管上での運動極性を利用して、微小流体デバイス内でナノスケールの対象分子などを大量・並列に操作し、分離することのできるナノアクチュエータの創出を目指した。微小管の極性配置技術を確立し、二種類のモータタンパク質が極性に従って効率よく運動する系を微小流体デバイス内で構築した。

Research Products

• [Journal Article] Versatile Microfluidic Total Internal Reflection (TIR)-based Devices: Application to Microbeads Velocity Measurement and Single Molecule Detection with Upright and Inverted Microscope (2009)
  • Author(s): N. C. H. Le, R. Yokokawa, D. V. Dao, T. D. Nguyen, J. Wells, and S. Sugiyama,
  • Journal Title: Lab Chip vol. 9 Pages: 244-250
  • Peer Reviewed
• [Journal Article] ersatile Microfluidic Total Internal Reflection (TIR)-based Devices : Application to Microbeads Velocity Measurement and Single Molecule Detection with Upright and Inverted Microscope (2009)
  • Author(s): N. C. H. Le, R. Ybkokawa, 他
  • Journal Title: Lab Chip 9 Pages: 244-250
  • Peer Reviewed
• [Journal Article] DNA molecule manipulation by motor proteins for its evaluation in single molecule level (2008)
  • Author(s): R. Yokokawa, J. Miwa, M. C. Tarhan, H. Fujita, and M. Kasahara
  • Journal Title: Anal. Bioanal. Chem vol. 391 Pages: 2735-43
  • Peer Reviewed
• [Journal Article] Simultaneous and Bidirectional Transport of Kinesin-Coated Microspheres and Dynein-Coated Microspheres on Polarity-Oriented Microtubules (2008)
  • Author(s): R. Yokokawa, M. C. Tarhan, T. Kon, and H. Fujita
  • Journal Title: Biotechnol. Bioeng vol. 101 Pages: 1-8
  • Peer Reviewed
• [Journal Article] Simultaneous and Bidirectional Transport of Kinesin-Coated Microspheres and Dynein-Coated Microspheres on Polarity-Oriented Microtubules (2008)
  • Author(s): R. Ybkokawa, M. C. Tarhan, 他
  • Journal Title: Biotechnol. Bioeng 101 Pages: 1-8
  • Peer Reviewed
• [Journal Article] DNA molecule manipulation by motor proteins for its evaluation in single molecule level (2008)
  • Author(s): R. Yokokawa, J. Miwa, 他
  • Journal Title: Anal. Bioanal. Chem. 391 Pages: 2735-2743
  • Peer Reviewed
• [Journal Article] 遺伝子組換えタンパク質評価のための細胞破砕デバイスと精製-1分子アッセイデバイス (2008)
  • Author(s): 中山鉄矢、横川隆司他
  • Journal Title: 電気学会E部門誌 128 Pages: 167-175
  • NAID: 10021135221
  • Peer Reviewed
• [Journal Article] Polarity Orientation of Microtubules Utilizing Dynein-Based Gliding Assay (2008)
  • Author(s): R. Yokokawa, T. Murakami他
  • Journal Title: Nanotechnology 19 Pages: 125505-125505
  • Peer Reviewed
• [Journal Article] Design, Simulation and Fabrication of a Total Internal Reflection (Tir)-Based Chip for Highly Sensitive Fluorescent Imaging (2007)
  • Author(s): N.C.H. Le, R. Yokokawa他
  • Journal Title: J. Micromech. Microeng. 17 Pages: 1139-1146
  • Peer Reviewed
• [Journal Article] Transcriptome analysis device based on liquid phase detection by fluor escently labeled nucleic acid probes (2007)
  • Author(s): R. Yokokawa, S. Tamaoki他
  • Journal Title: Biomed. Microdev. 9 Pages: 869-875
  • Peer Reviewed
• [Presentation] Monolithic Multicolor Total Internal Reflection (Tir)-Based Chip for Highly-Sensitive Multifluorescence Detection and Imaging (2009)
  • Author(s): N. C. H. Le, D. V. Dao, R. Yokokawa, J. Wells, and S. Sugiyama
  • Organizer: The 22nd IEEE International Conference on Micro Electro Mechanical Systems (MEMS2009)
  • Place of Presentation: Sorrento, Italy
• [Presentation] Kinesin-based transportation of hydrophobic and hydrophilic micro-containers (2008)
  • Author(s): C. Bottier, M.C. Tarhan, J. Fattaccioli, R. Yokokawa, D. Collard, H. Fujita
  • Organizer: International Symposium on Microchemistry and Microsystems (ISMM2008)
  • Place of Presentation: Kyoto, Japan
• [Presentation] Development and Application of Tir-Based Chip Based on Mems Thechnology (2008)
  • Author(s): R. Yokokawa, N. C. H. Le, D. V. Dao, J. C. Wells, and S. Sugiyama
  • Organizer: The first International Symposium on Micro/Nano Systems Technology
  • Place of Presentation: Hanoi, VietNam
• [Presentation] Kinesin-Based Transportation and Electrofusion of Lipid Vesicles (2008)
  • Author(s): C. Bottier, M. C. Tarhan, D. Collard, R. Yokokawa, and H. Fujita
  • Organizer: 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences (・TAS2008)
  • Place of Presentation: San Diego, USA
• [Presentation] Isolation and Manipulation of Single Microtubule by Silicon Microtweezers (2008)
  • Author(s): M. C. Tarhan, D. Collard, C. Bottier, R. Yokokawa, M. Hosogi, G. Hashiguchi, and H. Fujita,
  • Organizer: 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences
  • Place of Presentation: San Diego, USA
• [Presentation] Effective Dilution of Protein for Single Molecule Assay in an Integrated Assay Device (2008)
  • Author(s): T. Nakayama, K. Tabata, H. Noji, and R. Yokokawa
  • Organizer: 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences (・TAS2008)
  • Place of Presentation: San Diego, USA
• [Presentation] Dual-fluorescence Detection and Imaging with Dual-color Evanescent Excitation (EE)-based Chip (2008)
  • Author(s): N. C. H. Le, R. Yokokawa, D. V. Dao, J. Wells, and S. Sugiyama
  • Organizer: International Symposium on Microchemistry and Microsystems (ISMM2008)
  • Place of Presentation: Kyoto, Japan
• [Presentation] Development and Application of Tir-Based Chip Based on Mems Thechnology (2008)
  • Author(s): R. Yokokawa, N. C. H. Le, D. V. Dao, J. C. Wells, and S. Sugiyama
  • Organizer: The first International Symposium on Micro/Nano Systems Technology
  • Place of Presentation: Hanoi, Viet Nam
• [Presentation] Highly-Sensitive Dual-Fluorescence Detection and Imaging with Integrated Dual-Color Total Internal Reflection (Tir)-Based Chip (2008)
  • Author(s): N. C. H. Le, D. V. Dao, R. Yoko kawa, J. Wells, and S. Sugiyama
  • Organizer: IEEE Int'l Symposium on Micro-Nano Mechatronics and Human Science (IEEE MHS 2008)
  • Place of Presentation: Nagoya, Japan
• [Presentation] A Dual-Color Total Internal Reflection (Tir)-Based Chip for Simultaneous Detection of Two Fluorophores (2008)
  • Author(s): N. C. H. Le, D. V. Dao, R. Yoko kawa, J. C. Wells, and S. Sugiyama
  • Organizer: The 7th Annual IEEE Conference on Sensors (SE NSORS2008)
  • Place of Presentation: Lecce, Italy
• [Presentation] Kinesin-Based Transportation and Electrofusion of Lipid Vesicles (2008)
  • Author(s): C. Bottier, M. C. Tarhan, D. Collard, R. Yokokawa, and H. Fujita
  • Organizer: 12th International Confbrence on Miniaturized Systems for Chemistry and Lifb Sciences(μTAS2008)
  • Place of Presentation: San Diego, USA
• [Presentation] Isolation and Manipulation of Single Microtubule by Silicon Microtweezer (2008)
  • Author(s): M. C. Tarhan, D. Collard, C. Bottier, R. Yokokawa, M. Hosogi, G. Hashiguchi, and H. Fujita
  • Organizer: 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences(μTAS2008)
  • Place of Presentation: San Diego, USA
• [Presentation] Effective Dilution of Protein for Single Molecule Assay in an Integrated Assay Device (2008)
  • Author(s): T. Nakayama, K. Tabata, H. Noji. and R. Yokokawa
  • Organizer: 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences(μTAS2008)
  • Place of Presentation: San Diego, USA
• [Presentation] Integration of Evanescent Excitation (Ee)-Based Chip with Microfluidic Channels for Upright and Inverted Microscope Observations (2008)
  • Author(s): N. C. H. Le, R. Yokokawa, D. V. Dao, T. D. Nguyen, J. Wells, and S. Sugiyama
  • Organizer: 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences(μTAS2008)
  • Place of Presentation: San Diego, USA
• [Presentation] Transport of Functionalized Microneedles by Gliding Assay-Based Molecular System (2008)
  • Author(s): M. C. Tarhan, R. Yokokawa, C. Bottier, E. Sarajlic, and H. Fujita
  • Organizer: The 4th Asia- Pacific Conference on Transducers and Micro-Nano Technology (APCOT MNT2008)
  • Place of Presentation: Tainan, Taiwan
• [Presentation] Polarity-Selected Separation of Single Microtubule by Silicon Microtweezers (2008)
  • Author(s): M. C. Tarhan, D. Collard, R. Yokokawa, C. Bottier, M. Hosogi, G. Hashiguchi, and H. Fujita
  • Organizer: 第18回化学とマイクロナノシステム研究会(18th Cheminas)
  • Place of Presentation: 京都
• [Presentation] DNA分子のモータタンパク質による個別操作と制限酵素による1分子評価 (2008)
  • Author(s): 横川隆司, Tarhan M. C., 藤田博之, 笠原賢洋
  • Organizer: 第25回「センサ・マイクロマシンと応用システム」シンポジウム
  • Place of Presentation: 那覇
• [Presentation] 1分子MicroTASにおける層流拡散を用いたタンパク質評価条件の最適化 (2008)
  • Author(s): 中山鉄矢, 田端和仁, 野地博行, 横川隆司
  • Organizer: 第25回「センサ・マイクロマシンと応用システム」シンポジウム
  • Place of Presentation: 那覇
• [Presentation] Design, Analysis and Fabrication of a Dual-color Total Internal Reflection (TIR)-based Chip for Simultaneous Detection of Two Fluorophores (2008)
  • Author(s): Le Nam Cao Hoai, Yokokawa Ryuji, Dao Dzung Viet, Wells John, Sugiyama Susumu
  • Organizer: 第25回「センサ・マイクロマシンと応用システム」シンポジウム
  • Place of Presentation: 那覇
• [Presentation] Integration of Micromachined Structures With Kinesin Based Transport System (2008)
  • Author(s): Tarhan M. C., 横川隆司, 藤田博之
  • Organizer: 第17回化学とマイクロナノシステム研究会(17th Cheminas)
  • Place of Presentation: 福岡
• [Presentation] Measurement Near-wall Velocity Fields of Fluorescent Nanoparticles Transported in Microchannel Utilizing Integrated Evanescent Excitation (EE)-based Chip (2008)
  • Author(s): Le Nam Cao Hoai, Yokokawa Ryuji, Dao Dzung Viet, Wells John, Sugiyama Susumu
  • Organizer: 第17回化学とマイクロナノシステム研究会(17th Cheminas)
  • Place of Presentation: 福岡
• [Presentation] Bidirectional Transport of kinesin or Dynein-Coated Microspheres on Polar Oriented Microtubules (2008)
  • Author(s): R. Yokokawa
  • Organizer: MEMS2008
  • Place of Presentation: Tucson, USA
• [Presentation] Single DNA Molecule Manipulation by a Self-Assembled Motor Protein System (2008)
  • Author(s): R. Yokokawa
  • Organizer: MEMS2008
  • Place of Presentation: Tucson, USA
• [Presentation] A Total Internal Reflection (Tir)-Based Chip for Ultra-Sensitive Fluor escent Sensing (2007)
  • Author(s): N.C.H. Le
  • Organizer: TRANSDUCERS2007
  • Place of Presentation: Lyon, France
• [Presentation] A Monolithic Evanescent Excitation (Ee)-Based Biochip for Highlysensiive and Real-Time Fluorescent Detection (2007)
  • Author(s): D.V. Dao
  • Organizer: MicroTAS2007
  • Place of Presentation: Paris, France
• [Presentation] On-Chip Single Molecule Assay Device Integrating Cell Lysis and Protein Extraction-Purification-Assay Components for Genetically Engineered Proteins (2007)
  • Author(s): T. Nakayama
  • Organizer: MicroTAS2007
  • Place of Presentation: Paris, France
• [Presentation] Development of an Integrated Microfluidic Total Internal Reflection (Tir)-Based Chip for Nano-Particle Image Velocimetry (Nano-Piv) (2007)
  • Author(s): N.C.H. Le
  • Organizer: SENSORS2007
  • Place of Presentation: Atlanta, USA
• [Presentation] MOLECULAR MOTORS BASED SELECTIVE TRANSPORTATION OF SORTED TARGET MOLECULES (2007)
  • Author(s): Tarhan M.C.
  • Organizer: 第16回化学とマイクロナノシステム研究会
  • Place of Presentation: つくば
• [Presentation] モータタンパク質とDNAの自己組織的結合による分子操作技術 (2007)
  • Author(s): 横川 隆司
  • Organizer: 第16回化学とマイクロナノシステム研究会
  • Place of Presentation: つくば
• [Presentation] Single λ-DNA Imaging with Integrated Evanescent Excitation (EE)-based Chip (2007)
  • Author(s): 横川 隆司
  • Organizer: 第16回化学とマイクロナノシステム研究会
  • Place of Presentation: つくば
• [Presentation] キネシンとダイニンによる微小管の極性配向とマイクロビーズの双方向搬送 (2007)
  • Author(s): 横川 隆司
  • Organizer: 第24回「センサ・マイクロマシンと応用システム」シンポジウム
  • Place of Presentation: 東京
• [Presentation] 遺伝子組換えタンパク質評価のための1分子アッセイデバイス (2007)
  • Author(s): 中山 鉄矢
  • Organizer: 第15回化学とマイクロナノシステム研究会
  • Place of Presentation: 仙台
• [Patent(Industrial Property Rights)] 蛍光顕微鏡用全反射バイオチップ及びその製法、並びに蛍光顕微鏡用全反射バイオチップアセンブリ (2008)
  • Inventor(s): 杉山進、横川隆司、ジョンシーウェルズ、ナムカオホアイレ、ズンベトダオ
  • Industrial Property Rights Holder: 学校法人立命館
  • Industrial Property Number: 2008-119835
  • Filing Date: 2008-05-01
• [Patent(Industrial Property Rights)] 蛍光顕微鏡用全反射バイオチップ及びその製法、並びに蛍光顕微鏡用全反射バイオチップアセンブリ (2007)
  • Inventor(s): 杉山進、横川隆司、ジョンシーウェルズ、ナムカオホアイレ、ズンベトダオ
  • Industrial Property Rights Holder: 学校法人立命館
  • Industrial Property Number: 2007-121901
  • Filing Date: 2007-05-02
• [Patent(Industrial Property Rights)] 蛍光顕微鏡用全反射バイオチップ及びその製法、並びに蛍光顕微鏡用全反射バイオチップアセンブリ (2007)
  • Inventor(s): ナム カオ ホアイレ、ズン ベト ダオ、ジョン シー ウェルズ、横川 隆司、杉山 進
  • Industrial Property Rights Holder: 学校法人立命館
  • Industrial Property Number: 2007-121901
  • Filing Date: 2007-05-02