Geometrical influences of nanospace on DNA separation

• Project/Area Number: 19710115
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Microdevices/Nanodevices
• Research Institution: Nagoya University
• Principal Investigator: KAJI Noritada Nagoya University, 大学院・工学研究科, 助教 (90402479)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,570,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥2,400,000 (Direct Cost: ¥2,400,000)
• Keywords: マイクロ化学システム / ナノ構造体 / ナノバイオ / マイクロ・ナノデバイス / DNA

Research Abstract

本研究は、超微細加工技術にて作製したナノピラーチップを用いて、精密に制御されたナノ空間の幾何学的特性がDNAの分離分析へ及ぼす影響について詳細は検討を行った。その結果、ナノ空間の幾何学的パターンが、DNAの分離モードを支配していることを明らかとし、その分離メカニズムの理論的モデルを構築した。さらにこのナノピラーチップをDNAだけではなく、タンパク質への分離に応用することに成功した。

Research Products

• [Journal Article] Baba, Y., Dynamic Cross-Linking Effect of Mg(2+) To Enhance Sieving Properties of Low-Viscosity Poly(vinylpyrrolidone) Solutions for Microchip Electrophoresis of Proteins. (2008)
  • Author(s): Mohamadi, M. R.; Kaji, N.; Tokeshi, M.; Baba, Y.
  • Journal Title: Anal. Chem. 80(1) Pages: 312-316
  • Peer Reviewed
• [Journal Article] Quantitative evaluation of dynamic coating on plastic microchips for preventing protein adsorption (2008)
  • Author(s): Mohamadi, M. R.; Yasui, T.; Kaji, N.; Tokeshi, M.; Baba, Y.
  • Journal Title: The proceedings of μTAS 2008 1 Pages: 495-497
  • Peer Reviewed
• [Journal Article] Nanopillar chips arranged in tilted array pattern for fast separation of DNA and proteins (2008)
  • Author(s): Yasui, T.; kaji, N.; Mohamadi, M. R.; Ogawa, R.; Hashioka, S.; Tokeshi, M.; Horiike, Y.; Baba, Y
  • Journal Title: The proceedings of μTAS 2008 1 Pages: 432-434
  • Peer Reviewed
• [Journal Article] Dynamic Cross-Linking Effect of Mg (2+) To Enhance Sieving Properties of Low-Viscosity Poly (vinylpyrrolidone) Solutions for Microchip Electroph oresis of Proteins (2008)
  • Author(s): M. R. Mohamadi, et.al.
  • Journal Title: Anal. Chem. 80 Pages: 312-316
  • Peer Reviewed
• [Journal Article] Quantitative evaluation of dynamic coating on plastic microchips for preventing protein adsorption (2008)
  • Author(s): M. R. Mohamadi, et.al.
  • Journal Title: The proceedings of μTAS 2008 1 Pages: 495-497
  • Peer Reviewed
• [Journal Article] Nanopillar chips arranged in tilted array pattern for fast separation of DNA and proteins (2008)
  • Author(s): T. Yasui, et.al.
  • Journal Title: The proceedings of μTAS 2008 1 Pages: 432-434
  • Peer Reviewed
• [Journal Article] Influences of Electroosmotic Flows in Nanopillar Chips on DNA Separation : Experimental Results and Numerical Simulations. (2007)
  • Author(s): Kaji, N.; Oki, A.; Ogawa, R.; Takamura, Y.; Nishimoto, T.; Nakanishi, H.; Horiike, Y.; Tokeshi, M.; Baba, Y
  • Journal Title: Israel Journal of Chemistry 47(2) Pages: 161-169
  • Peer Reviewed
• [Journal Article] Single-molecule measurements with a single quantum dot. (2007)
  • Author(s): Kaji, N.; Tokeshi, M.; Baba, Y
  • Journal Title: Chem. Rec. 7(5) Pages: 295-304
  • Peer Reviewed
• [Journal Article] Quantum dots for single bio-molecule imaging (2007)
  • Author(s): Kaji, N.; Tokeshi, M.; Baba, Y
  • Journal Title: Anal. Sci. 23(1) Pages: 21-4
  • NAID: 10018463942
  • Peer Reviewed
• [Journal Article] DNA separation by square patterned nanopillar chips (2007)
  • Author(s): Yasui, T.; Kaji, N.; Ogawa, R.; Hashioka, S.; Tokeshi, M.; Horiike, Y.; Baba, Y.
  • Journal Title: The proceedings of μTAS 2007 2 Pages: 1207-1209
  • Peer Reviewed
• [Journal Article] Quantum Dots for Singel Bio-Molecule Imaging (2007)
  • Author(s): N. Kaji, et. al.
  • Journal Title: Anal. Sci. 23 Pages: 21-24
  • Peer Reviewed
• [Journal Article] Single Molecule Measurements with a Single Quantum Dot (2007)
  • Author(s): N. Kaji, et. al.
  • Journal Title: The Chemical Record 7 Pages: 295-304
  • Peer Reviewed
• [Journal Article] DNA Separation by Square Patterned Nanopillar Chips (2007)
  • Author(s): T. Yasui, et. al.
  • Journal Title: The proceedings of Micro Total Analysis Systems 2007 2 Pages: 1207-1209
  • Peer Reviewed
• [Journal Article] Influences of Electroosmotic Flows in Nanopillar Chip on DNA Separati on: Experimental Results and Numerical Simulations
  • Author(s): N. Kaji, et. al.
  • Journal Title: Israel J. Chem., 2008(印刷中)
  • Peer Reviewed
• [Presentation] DNA and protein separations by nanopillar array structures (2009)
  • Author(s): N. Kaji
  • Organizer: Fifth International Conference on Molecular Electronics and Bioelectronics (M&BE5)
  • Place of Presentation: 宮崎
  • Year and Date: 2009-03-16
• [Presentation] DNA and protein separations by nanopillar array structures (2009)
  • Author(s): N. Kaji
  • Organizer: Fifth International Conference on Molecular Electronics and Bioelectronics (M & BE 5)
  • Place of Presentation: 宮崎
  • Year and Date: 2009-03-16
• [Presentation] ナノ空間を利用した生体高分子解析法の開発, (2008)
  • Author(s): 加地範匡
  • Organizer: 第39回中部化学関係学協会支部連合秋季大会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-11-08
• [Presentation] ナノ空間を利用した生体高分子解析法の開発 (2008)
  • Author(s): 加地範匡
  • Organizer: 第39回中部化学関係学協会支部連合秋季大会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-11-08
• [Presentation] Non-equilibrium DNA transport in nanopillar chips toward high-throughput DNA separation (2008)
  • Author(s): N. Kaji
  • Organizer: Micro- and Nano Technologies in Medicine - a Swedish-Japanese collaborative workshop -
  • Place of Presentation: Uppsala, Sweden
  • Year and Date: 2008-10-22
• [Presentation] Non-equilibrium DNA transport in nanopillar chips toward high-throughput DNA separation (2008)
  • Author(s): N. Kaji
  • Organizer: Micro-and Nano Technologies in Medicine-a Swedish-Japanese collaborative workshop
  • Place of Presentation: Uppsala, Sweden
  • Year and Date: 2008-10-22
• [Presentation] ナノ空間中におけるDNAのダイナミクス (2008)
  • Author(s): 加地範匡
  • Organizer: ナノバイオ若手ネットワーキングシンポジウム
  • Place of Presentation: 三島
  • Year and Date: 2008-06-12
• [Presentation] 微小空間における1分子酵素反応の解析 (2008)
  • Author(s): 加地範匡
  • Organizer: 第69回分析化学討論会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-05-16
• [Presentation] Separation Sciences with Nano-fabricated Structures (2008)
  • Author(s): N. Kaji
  • Organizer: 日本化学会第88年会春季年会
  • Place of Presentation: 東京
  • Year and Date: 2008-03-29
• [Presentation] DNA molecular behavior in nanowall array structures (2008)
  • Author(s): N. Kaji
  • Organizer: Microscale bioseparations methods methods for systems biology (MSB2008)
  • Place of Presentation: Berlin, Germany
  • Year and Date: 2008-03-10
• [Book] Nanopillars and nanoballs for DNA analysis (2009)
  • Author(s): Kaji, N.; Tokeshi, M.; Baba, Y.
  • Publisher: RSC Publishing
• [Book] Nanopillars and nanoballs for DNA analysis (2009)
  • Author(s): N. Kaji, et.al.
  • Publisher: RSC Publishing
• [Book] バイオインダストリー (2007)
  • Author(s): 加地範匡、馬場嘉信
  • Publisher: シーエムシー出版
• [Book] バイオインダストリー (2007)
  • Author(s): 加地範匡, 他
  • Publisher: シーエムシー出版
• [Book] MEMS/NEMS工学大系
  • Author(s): 加地範匡、馬場嘉信
  • Publisher: テクノシステム(印刷中)
• [Book] MEMS/NEMS工学大系
  • Author(s): 加地範匡, 他
  • Publisher: テクノシステム(印刷中)