APPRICATION OF MICRON-SPIKED ELECTRODES PRODUCED BY THE PHASE TRANSFORMATION PHENOMENON OF STAINLESS STEEL FOR GENE TRANSFER DEVICE

• Project/Area Number: 19710116
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Microdevices/Nanodevices
• Research Institution: Kyoto University
• Principal Investigator: MIYANO Naoki Kyoto University, 工学研究科, 講師 (40363353)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,720,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2007: ¥1,900,000 (Direct Cost: ¥1,900,000)
• Keywords: マイクロ化学システム / 遺伝子導入デバイス / 自己組織化 / マイクロ・ナノデバイス / ステンレス鋼 / エレクトロポレーション / μ-TAS / マイクロ・ナイデバイス

Research Abstract

二相ステンレス鋼を用いた精密熱処理とエッチング技術によるニードル形状の製作,および,そのニードル形状上での細胞培養に成功した.これらを踏まえ,より高効率な遺伝子導入を目指すナノ・ニードル基板エレクトロポレーションの開発を推し進め,その結果を論文投稿するまでに至った.

Research Products

• [Journal Article] Gene transfer device utilizing micron-spiked electrodes produced by the self-organization phenomenon of Fe-alloy (2008)
  • Author(s): Naoki Miyano, Keisuke Fujii, Yuuki Inoue, Yuji Teramura, Hiroo Iwata and Hidetoshi Kotera
  • Journal Title: Lab. Chip. 8 Pages: 1104-1109
  • Peer Reviewed
• [Journal Article] Prediction of Precipitate Morphology by Atomic Matchin Model and FEM analysis with Consideration od Anistropy Elastic Strain in a BCC/HCP system (2008)
  • Author(s): N. Midyano, A. Takahashi, J. Miyazaki and K. Ameyama
  • Journal Title: Material Transactions 19 Pages: 471-478
  • Peer Reviewed
• [Journal Article] FEM analysis based on the 3D-NCS model for precipitate morphology in the BCC/HCP system (2008)
  • Author(s): N. Miyano, K. Ameyama, N. Hirano, Y. Takao
  • Journal Title: Materials Science and Engineering A 480 Pages: 464-471
  • Peer Reviewed
• [Journal Article] Gene transfer device utilizing micron-spiked electrodes produced by the self-organization phenomenon of Fe-alloy (2008)
  • Author(s): Naoki Miyano, Keisuke Fujii, Yuuki Inoue, Yuji Teramura, Hiroo Iwata and Hidetoshi Kotera
  • Journal Title: Lab. Chip 8 Pages: 1104-1109
  • Peer Reviewed
• [Journal Article] Prediction of Precipitate Morphology by Atomic Matchin Model and FEM analysis with Consideration od Anistropy Elastic Strain in a BCC/HCP system (2008)
  • Author(s): N. Miyano, A. Takahashi, J. Miyazaki and K. Ameyama
  • Journal Title: Material Transactions 19 Pages: 471-478
  • Peer Reviewed
• [Journal Article] Ti-15V-3Cr-3Sn-3Al合金における三角錐形状α析出相の結晶学的考察日本金属学会誌71 (2007)
  • Author(s): 宮野公樹,則村貴洋,稲葉輝彦,飴山惠
  • Journal Title: J. Japan Inst. Metals Vol. 71, No. 5 Pages: 469-475
  • Peer Reviewed
• [Journal Article] Ti-15V-3Cr-3Sn-3Al合金における三角錐形状α析出相の結晶学的考察 (2007)
  • Author(s): 宮野公樹, 則村貴洋, 稲葉輝彦, 飴山 惠
  • Journal Title: 日本金属学会誌 71 Pages: 469-475
  • NAID: 10020136964
  • Peer Reviewed
• [Journal Article] Development of Gene Transfer Device with a Submicron-Needle Produced by a Self-Organization Phenomenon in an Fe-alloy (2007)
  • Author(s): Naoki Miyano, Keisuke Fujii, Yuuki Inoue, Yuji Teramura, Hiroo Iwata and Hidetoshi Kotera
  • Journal Title: Proc. Intelligent Processing and Manufacturing of Materials (2007)
• [Journal Article] Development of Deformable Micropillar Array using Magnetic Particles and Elastic Material (2007)
  • Author(s): Fujio Tsumori, Naoki Miyano, Akio Fukui, Koji Sagawa, Hidetoshi Kotera
  • Journal Title: Proc. Intelligent Processing and Manufacturing of Materials (2007)
• [Journal Article] Deformable Micro Pillar Array with Magnetic Particles and Elastic Material (2007)
  • Author(s): Fujio Tsumori, Naoki Miyano, Akio Fukui, Koji Sagawa, Hidetoshi Kotera
  • Journal Title: Proc. Int. Conf. Computational Plasticity
• [Presentation] AN APPLICATION OF A STAINLESS SUBMICRON NEEDLE FOR THE GENE TRANSFER DEVICE (2008)
  • Author(s): N. Miyano, Y. Inoue, Y. Teramura, N. Nishiguchi, H. Iwata and H. Kotera
  • Organizer: Asia-Pacific Conference on Transducers and Micro-Nano Technology
  • Place of Presentation: Taiwan
• [Presentation] APPRICATION OF MICRON-SPIKED ELECTRODES PRODUCED BY THE PHASE TRANSFORMATION PHENOMENON OF STAINLESS STEEL FOR GENE TRANSFER DEVICE (2008)
  • Author(s): N. Miyano, M. Morieda, Y. Inoue, Y. Teramura, F. Tsumori, H. Iwata and H. Kotera
  • Organizer: micro-TAS
  • Place of Presentation: Sandiego
• [Presentation] Shape-Dependent Strain of Precipitate Morphology Investigated by FEM Analysis in a BCC/HCP system (2008)
  • Author(s): N. Miyano, Akihiro Takahashi and Kei Ameyama
  • Organizer: ISPMA 2008
  • Place of Presentation: Prague
• [Presentation] Prediction of Precipitate Morphology by Atomic Matching Model and FEM Analysis with Consideration of Anisotropy Elastic Strain in a BCC/HCP system (2008)
  • Author(s): Akihiro Takahash, N. Miyano and Kei Ameyama
  • Organizer: 17th International Federation for Heat Treatment and Surface Engineering
  • Place of Presentation: 神戸
• [Presentation] AN APPLICATION OF A STAINLESS SUBMICRON NEEDLE FOR THE GENE TRANSFER DEVICE (2008)
  • Author(s): N. Miyano, Y. Inoue, Y. Teramura, N. Nishiguchi, H. Iwata and H. Kotera
  • Organizer: Asia-Pacific Conference on Transducers and Micro-Nano Technology
  • Place of Presentation: Itaiwan
• [Presentation] Prediction of Precipitate Morphology by Atomic Matching Model and FEM Analysis with Consideration of Anisotropy Elastic Strain in a BCC/HCP system (2008)
  • Author(s): Akihiro Takahash, N. Miyano and Kei Ameyama
  • Organizer: 17th International Federation for Heat Treatment and Surface Engineering
  • Place of Presentation: Kobe
• [Presentation] 自己組織化による"ステンレス製"ナノ・ニードル構造体を用いた遺伝子導入デバイスの開発 (2007)
  • Author(s): 宮野公樹,藤井恵介,井上祐貴,寺村裕治,津守不二夫,岩田博夫,小寺秀俊
  • Organizer: 第24回「センサ・マイクロマシンと応用システム」シンポジウム
  • Place of Presentation: 船橋
  • Year and Date: 2007-10-16
• [Presentation] 二相ステンレス鋼における析出相を活用したナノ・ニードル基板とその応用 (2007)
  • Author(s): 宮野公樹,藤井恵介,津守不二夫,小寺秀俊
  • Organizer: 07年秋期金属学会講演大会
  • Place of Presentation: 岐阜大学
  • Year and Date: 2007-09-19
• [Presentation] Deformable Micro Pillar Array with Magnetic Particles and Elastic Material (2007)
  • Author(s): Fujio Tsumori, Naoki Miyano, Akio Fukui, Koji Sagawa, Hidetoshi Kotera
  • Organizer: Int. Conf. Computational Plasticit
  • Place of Presentation: バルセロナ
  • Year and Date: 2007-09-06
• [Presentation] Development of Gene Transfer Device with a Submicron-Needle Produced by a Self-Organization Phenomenon in an Fe-alloy (2007)
  • Author(s): Naoki Miyano, Keisuke Fujii, Yuuki Inoue, Yuji Teramura, Hiroo Iwata and Hidetoshi Kotera
  • Organizer: The 6th International Conference on Intelligent Processing and Manufacturing of Materials
  • Place of Presentation: イタリア
  • Year and Date: 2007-06-27
• [Presentation] Development of Deformable Micropillar Array using Magnetic Particles and Elastic Material (2007)
  • Author(s): Fujio Tsumori, Naoki Miyano, Akio Fukui, Koji Sagawa, Hidetoshi Kotera
  • Organizer: The 6th International Conference on Intelligent Processing and Manufacturing of Materials
  • Place of Presentation: イタリア
  • Year and Date: 2007-06-27
• [Patent(Industrial Property Rights)] 二相ステンレス鋼部材とその製造方法およびその表面処理方法,固体高分子型燃料電池とそのセパレータ、導通部材並びにバイオデバイス (2008)
  • Inventor(s): 川浦宏之、鈴木憲一、宮野公樹、射場英紀、高尾尚史
  • Industrial Property Rights Holder: 京都大学、豊田中央研究所、トヨタ自動車
  • Industrial Property Number: 2008-037407
  • Filing Date: 2008-02-06
• [Patent(Industrial Property Rights)] 二相ステンレス鋼部材とその製造方法およびその表面処理方法、固体高分子型燃料電池とそのセパレータ、導通部材並びにバイオデバイス (2008)
  • Inventor(s): 川浦宏之、鈴木憲一、宮野公樹、射場英紀、高尾尚史
  • Industrial Property Rights Holder: 京都大学、豊田中央研究所、トヨタ自動車
  • Industrial Property Number: 2008-037407
  • Filing Date: 2008-02-06