Design and synthesis of histone tail polypeptide library

• Project/Area Number: 19710181
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Living organism molecular science
• Research Institution: Tohoku University (2008); The University of Tokyo (2007)
• Principal Investigator: SAKAMOTO Seiji Tohoku University, 多元物質科学研究所, 助教 (30335228)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,600,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥2,300,000 (Direct Cost: ¥2,300,000)
• Keywords: 生体機能関連物質 / ポリペプチド / ライブラリ / エピジェネティクス / ヒストンテール / ライブリ / 分割型GFP

Research Abstract

本研究では様々なアミノ酸側鎖翻訳後修飾を位置特異的に施したヒストンテールポリペプチドライブラリの構築を行った。さらに、構築したライブラリを用いて、代表的クロマチン関連因子由来のブロモドメインやクロモドメインとの相互作用を解析した。また、得られた情報に基づき、ヒストン認識ドメインや人工の刺激応答部位を複合化した分割型 GFPを設計し、任意のタンパク質翻訳後修飾反応や外部刺激を特異的に検出可能な蛍光プローブを構築することに成功した。

Research Products

• [Journal Article] Design of Photoactivable Split-GFP by Incorporation of A Photolabile Linker (2009)
  • Author(s): S. Sakamoto, Y. Araki, T. Wada
  • Journal Title: Peptide Science 2008 Pages: 487-490
  • NAID: 10027166758
  • Peer Reviewed
• [Journal Article] Resin-Bound Peptide Catalyzed Asymmetric Synthesis under Aqueous Conditions (2009)
  • Author(s): K. Akagawa, H. Akabane, T. Fujiwara, T. Yamashita, S. Sakamotro, K. Kudo
  • Journal Title: Peptide Science 2008 Pages: 529-530
  • Peer Reviewed
• [Journal Article] Asymmetric transfer hydrogenation in aqueous media catalyzed by resin-supported peptide having polyleucine tether (2009)
  • Author(s): K. Akagawa, H. Akabane, S. Sakamoto, K. Kudo
  • Journal Title: Tetrahedron : Asymmetry 20 Pages: 461-466
  • Peer Reviewed
• [Journal Article] Cyclo[-His-His-] Derived C2-Symmetric Diketopiperazine as Chiral Ligand for Asymmetric Diels-Alder Reactions (2009)
  • Author(s): M. Furutani, S. Sakamoto, K. Kudo
  • Journal Title: Heterocycles 78 Pages: 1171-1176
  • NAID: 40016557910
  • Peer Reviewed
• [Journal Article] Design of Photoactivable Split-GFP by Incorporation of A Photolabile Linker (2009)
  • Author(s): Seiji Sakamoto, Yasuyuki Araki, Takehiko Wada
  • Journal Title: Peptide Science 2008 Pages: 487-490
  • NAID: 10027166758
  • Peer Reviewed
• [Journal Article] Resin-Bound Peptide Catalyzed Asymmetric Synthesis under Aqueous Conditions (2009)
  • Author(s): K. Akagawa, H. Akabane, T. Fujiwara, T. Yamashita, S. Sakamotro. K. Kudo
  • Journal Title: Peptide Science 2008 Pages: 529-530
  • Peer Reviewed
• [Journal Article] Asymmetric transfer hydrogenation in aqueous media catalyzed by resin-supported peptide having nolvleucine tether (2009)
  • Author(s): K. Akagawa, H. Akabane, S. Sakamoto. K. Kudo
  • Journal Title: Tetrahedron : Asymmetry 20 Pages: 461-466
  • Peer Reviewed
• [Journal Article] Cyclo[-His-His-] Derived C2-Symmetric Diketopiperazine as Chiral Lieand for Asymmetric Diels-Alder Reactions (2009)
  • Author(s): M. Furutani, S. Sakamoto, K. Kudo
  • Journal Title: Heterocycles 78 Pages: 1171-1176
  • Peer Reviewed
• [Journal Article] Supramolecular Control of Split-GFP Reassembly by Conjugation of β-Cyclodextrin and Coumarin Units (2008)
  • Author(s): S. Sakamoto, K. Kudo
  • Journal Title: J. Am. Chem. Soc 130 Pages: 9574-9582
  • Peer Reviewed
• [Journal Article] Organocatalytic Asymmetric Transfer Hydrogenation in Aqueous Media using Resin-Supported Peptide having Polyleucine (2008)
  • Author(s): K. Akagawa, H. Akabane, S. Sakamoto, . Kudo
  • Journal Title: Org. Lett 10 Pages: 2035-2037
  • Peer Reviewed
• [Journal Article] Glucose Responsive Two-step Release of Hydrogel-Immobilized Protein (2008)
  • Author(s): K. Murota, S. Sakamoto, K. Kudo
  • Journal Title: Chem. Lett 37 Pages: 582-583
  • NAID: 10021080928
  • Peer Reviewed
• [Journal Article] Construction of β-Cyclodextrin and Fluorophore Conjugated Split-GFP Whose Reassembly Is Accelerated by the Addition of Hydrophobic Guest Molecules (2008)
  • Author(s): S. Sakamoto, K. Kudo
  • Journal Title: Peptide Science 2007 Pages: 455-458
  • NAID: 10021907315
  • Peer Reviewed
• [Journal Article] Design and Characterization of Split-GFP Whose Reassembly Is Accelerated by Phosphorylation (2008)
  • Author(s): M. Kasahara, S. Sakamoto, K. Kudo
  • Journal Title: Peptide Science 2007 Pages: 459-463
  • NAID: 10021907320
  • Peer Reviewed
• [Journal Article] Supramolecular Control of Split-GFP Reassembly by Conjugation of β-Cyclodextrin and Coumarin Units (2008)
  • Author(s): Seiji Sakamoto, Kazuaki Kudo
  • Journal Title: J. Am. Chem. Soc 130 Pages: 9574-9582
  • Peer Reviewed
• [Journal Article] Organocatalytic Asymmetric Transfer Hydrogenation in Aqueous Media using Resin-Supported Peptide having Polyleucine (2008)
  • Author(s): Kengo Akagawa, Hajime Akabane, Seiji Sakamoto, Kazuaki Kudo
  • Journal Title: Org. Lett 10 Pages: 2035-2037
  • Peer Reviewed
• [Journal Article] Glucose Responsive Two-step Release of Hydrogel-Immobilized Protein (2008)
  • Author(s): Kazutoshi Murota, Seiii Sakamoto, Kazuaki Kudo
  • Journal Title: Chem. Lett 37 Pages: 582-583
  • NAID: 10021080928
  • Peer Reviewed
• [Journal Article] Construction of β-Cyclodextrin and Fluorophore Conjugated Split-GFP Whose Reassembly Is Accelerated by the Addition of Hydrophobic Guest Molecules (2008)
  • Author(s): Seiji Sakamoto, Kazuaki Kudo
  • Journal Title: Peptide Science 2007 Pages: 455-458
  • NAID: 10021907315
  • Peer Reviewed
• [Journal Article] Design and Characterization of Split-GFP Whose Reassembly Is Accelerated by Phosphorylation (2008)
  • Author(s): Mutsumi Kasahara, Seiji Sakamoto, Kazuaki Kudo
  • Journal Title: Peptide Science 2007 Pages: 459-463
  • NAID: 10021907320
  • Peer Reviewed
• [Journal Article] Reversible Immobilization of Protein into Hydrogel Using Designed Coiled-coil Peptides (2007)
  • Author(s): K. Murota, S. Sakamoto, K. Kudo
  • Journal Title: Chem. Lett 36 Pages: 1320-1321
  • NAID: 10019792050
  • Peer Reviewed
• [Journal Article] Orientation Control of Self-stacking D,L-Alternating Cyclic Octa-α-peptide through Multiple Electrostatic Interactions (2007)
  • Author(s): K. Murota, S. Sakamoto, K. Kudo
  • Journal Title: Chem. Lett 36 Pages: 1070-1071
  • NAID: 10019744724
  • Peer Reviewed
• [Journal Article] Reversible Immobilization of Protein into Hydrogel Using Designed Coiled-coil Peptides (2007)
  • Author(s): Kazutoshi Murota, Seiji Sakamoto, Kazuaki Kudo
  • Journal Title: Chemistry Letters 36 Pages: 1320-1321
  • NAID: 10019792050
  • Peer Reviewed
• [Journal Article] Orientation Control of Self-stacking D, L-Alternating Cyclic Octa-α-peptide through Multiple Electrostatic Interactions (2007)
  • Author(s): Kazutoshi Murota, Seiji Sakamoto, Kazuaki Kudo
  • Journal Title: Chemistry Letters 36 Pages: 1070-1071
  • NAID: 10019744724
  • Peer Reviewed
• [Presentation] 外部刺激応答機能を持つ分割型 GFP 変異体を用いた高感度検出システムへの応用 (2009)
  • Author(s): 坂本清志, 寺内美香, 荒木保幸, 和田健彦
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 船橋
  • Year and Date: 2009-03-29
• [Presentation] 外部刺激応答型緑色蛍光タンパク質の合成と蛍光検出への応用 (2009)
  • Author(s): 寺内美香, 坂本清志, 荒木保幸, 和田健彦
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 船橋
  • Year and Date: 2009-03-29
• [Presentation] 外部刺激応答機能を持つ分割型GFP変異体を用いた高感度検出システムへの応用 (2009)
  • Author(s): 坂本清志, 寺内美香, 荒木保幸, 和田健彦
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 船橋
  • Year and Date: 2009-03-29
• [Presentation] Cyvlo[His-His]型キラル配位子を用いた銅触媒不斉 Diels-Alder 反応 (2009)
  • Author(s): 古谷昌太, 坂本清志, 工藤一秋
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 船橋
  • Year and Date: 2009-03-28
• [Presentation] 塩基部配向規制因子としてフェニルボロン酸を有するペプチドリボ核酸 (PRNA) の RNA 錯体形成解離の pH による制御 (2009)
  • Author(s): 遠藤絵梨子, 下司慶一郎, 坂本清志,荒木保幸, 井上佳久, 和田健彦
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 船橋
  • Year and Date: 2009-03-28
• [Presentation] アルギニンおよびセリン含有 α-ペプチドリボ核酸の合成と DNA・RNA との相互作用 (2009)
  • Author(s): 小野寺恵子, 西尾明洋, 坂本清志, 荒木保幸, 井上佳久, 和田健彦
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 船橋
  • Year and Date: 2009-03-28
• [Presentation] 固相担持プロリル触媒を用いた分子内 Baylis-Hillman 反応 (2009)
  • Author(s): 赤川賢吾, 坂本清志, 工藤一秋
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 船橋
  • Year and Date: 2009-03-28
• [Presentation] レーザー励起による DNA-カチオン性ポルフィリン複合体の時間分解円二色性 (2009)
  • Author(s): 村上憤, 荒木保幸, 坂本清志, 和田健彦
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 船橋
  • Year and Date: 2009-03-28
• [Presentation] Cyvio [His-His]型キラル配位子を用いた銅触媒不斉Diels-Alder反応 (2009)
  • Author(s): 古谷昌太, 坂本清志, 工藤一秋
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 船橋
  • Year and Date: 2009-03-28
• [Presentation] 塩基部配向規制因子としてフェニルボロン酸を有するペプチドリポ核酸(PRNA)のRNA錯体形成解離のpHによる制御 (2009)
  • Author(s): 遠藤絵梨子, 下司慶一郎, 坂本清志, 荒木保幸, 井上佳久, 和田健彦
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 船橋
  • Year and Date: 2009-03-28
• [Presentation] アルギニンおよびセリン含有α-ペプチドリボ核酸の合成とDNA・RNAとの相互作用 (2009)
  • Author(s): 小野寺恵子, 西尾明洋, 坂本清志, 荒木保幸, 井上佳久, 和田健彦
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 船橋
  • Year and Date: 2009-03-28
• [Presentation] 固相担持プロリル触媒を用いた分子内Baylis-Hillman反応 (2009)
  • Author(s): 赤川賢吾, 坂本清志, 工藤一秋
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 船橋
  • Year and Date: 2009-03-28
• [Presentation] 外部刺激による緑色蛍光タンパク質の機能制御 (2008)
  • Author(s): 坂本清志
  • Organizer: 第11回生命化学研究会
  • Place of Presentation: 水上
  • Year and Date: 2008-11-28
• [Presentation] 光によって活性化される分割型緑色蛍光タンパク質の設計 (2008)
  • Author(s): 坂本清志, 荒木保幸, 和田健彦
  • Place of Presentation: 東京
  • Year and Date: 2008-10-29
• [Presentation] 樹脂固定化ペプチドを用いる水系溶媒中での触媒的不斉合成 (2008)
  • Author(s): 赤川賢吾, 赤羽 創, 藤原巧真, 山下拓大, 坂本清志, 工藤一秋
  • Place of Presentation: 東京
  • Year and Date: 2008-10-29
• [Presentation] 樹脂固定化ペプチドを用いる水系溶媒中での触媒的不斉合成 (2008)
  • Author(s): 赤川賢吾, 赤羽創, 藤原巧真, 山下拓大, 坂本清志, 工藤一秋
  • Organizer: 第45回ペプチド討論会
  • Place of Presentation: 東京
  • Year and Date: 2008-10-29
• [Presentation] 外部刺激応答機能を持つ分割型緑色蛍光タンパク質変異体の設計 (2008)
  • Author(s): 坂本清志, 和田健彦
  • Organizer: 第3回バイオ関連化学合同シンポジウム
  • Place of Presentation: 横浜
  • Year and Date: 2008-09-18
• [Presentation] β-シクロデキストリンと蛍光色素を複合化したGFP変異体の設計と半合成 (2008)
  • Author(s): 坂本清志, 工藤一秋
  • Organizer: 日本化学会第88春季年会
  • Place of Presentation: 東京
  • Year and Date: 2008-03-28
• [Presentation] レーザー励起によるDNA-カチオン性ポルフィリン複合体の時間分解円二色性 (2008)
  • Author(s): 村上憤, 荒木保幸, 坂本清志, 和田健彦
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 船橋
  • Year and Date: 2008-03-28
• [Presentation] Construction of β-Cyclodextrin and Fluorophore Conjugated Split-GFP Whose Reassembly Is Accelerated by the Addition of Hydrophobic Guest Molecules (2007)
  • Author(s): 坂本清志, 工藤一秋
  • Organizer: 第44回ペプチド討論会
  • Place of Presentation: 富山
  • Year and Date: 2007-11-08
• [Presentation] Design and Characterization of Split-GFP Whose Reassembly Is Accelerated by Phosphorylation (2007)
  • Author(s): 笠原睦美, 坂本清志, 工藤一秋
  • Organizer: 第44回ペプチド討論会
  • Place of Presentation: 富山
  • Year and Date: 2007-11-08
• [Presentation] シクロデキストリン複合化緑色蛍光タンパク質の設計 (2007)
  • Author(s): 坂本清志, 工藤一秋,
  • Organizer: 第22回生体機能関連化学シンポジウム・若手フォーラム
  • Place of Presentation: 仙台
  • Year and Date: 2007-09-30
• [Presentation] β-シクロデキストリンと蛍光色素を複合化した分割型GFP変異体の構築 (2007)
  • Author(s): 坂本清志, 工藤一秋
  • Organizer: 第22回生体機能関連化学シンポジウム
  • Place of Presentation: 仙台
  • Year and Date: 2007-09-30
• [Presentation] タンパク質翻訳後修飾反応の検出を指向した分割型GFP変異体の構築 (2007)
  • Author(s): 笠原睦美, 坂本清志, 工藤一秋
  • Organizer: 第22回生体機能関連化学シンポジウム
  • Place of Presentation: 仙台
  • Year and Date: 2007-09-29