Development of a non-destructive imaging probe for cell and tissue using bioluminescence
| Project/Area Number |
19710196
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Living organism molecular science
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
WU Chun National Institute of Advanced Industrial Science and Technology, セルエンジニアリング研究部門, 研究員 (90415646)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥3,870,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥570,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | ウミホタルルシフェラーゼ / ルシフェリン / 光イメージング / 個体イメージング / 生物発光 / ルシフェラーゼ / 発光系 |
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| Research Abstract |
We aimed to develop a new far-red luminescence imaging technology for visualization of disease specific antigens on cell surfaces in a living body. First, we conjugated a far-red fluorescent indocyanine derivative to biotinylated Cypridina luciferase. This conjugate produced a bimodal spectrum having long-wavelength bioluminescence emission in the far-red region as a result of bioluminescence resonance energy transfer. To generate a new far-red luminescent probe with targeting and imaging capabilities of tumors, we then linked this conjugate to an anti-human Dlk-1 monoclonal antibody via the biotin-avidin interaction. This far-red luminescent probe enabled us to obtain high-resolution microscopic images of Dlk-1-expressing Huh-7 live cells without an external light source, and to monitor the accumulation of this probe in tumor-bearing mice. Furthermore, we developed an ELISA system using Cypridina luciferase to detect bio-marker in vitro.
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Report
(4 results)
Research Products
(11 results)
