| Project/Area Number |
19710196
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Living organism molecular science
|
|---|
| Research Institution | National Institute of Advanced Industrial Science and Technology |
|---|
Principal Investigator |
WU Chun National Institute of Advanced Industrial Science and Technology, セルエンジニアリング研究部門, 研究員 (90415646)
|
|---|
| Project Period (FY) |
2007 – 2009
|
| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥3,870,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥570,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥1,400,000 (Direct Cost: ¥1,400,000)
|
|---|
| Keywords | ウミホタルルシフェラーゼ / ルシフェリン / 光イメージング / 個体イメージング / 生物発光 / ルシフェラーゼ / 発光系 |
|---|
| Research Abstract |
We aimed to develop a new far-red luminescence imaging technology for visualization of disease specific antigens on cell surfaces in a living body. First, we conjugated a far-red fluorescent indocyanine derivative to biotinylated Cypridina luciferase. This conjugate produced a bimodal spectrum having long-wavelength bioluminescence emission in the far-red region as a result of bioluminescence resonance energy transfer. To generate a new far-red luminescent probe with targeting and imaging capabilities of tumors, we then linked this conjugate to an anti-human Dlk-1 monoclonal antibody via the biotin-avidin interaction. This far-red luminescent probe enabled us to obtain high-resolution microscopic images of Dlk-1-expressing Huh-7 live cells without an external light source, and to monitor the accumulation of this probe in tumor-bearing mice. Furthermore, we developed an ELISA system using Cypridina luciferase to detect bio-marker in vitro.
|
