Does inbreeding depression affect the extinction risk of wild populations of Primula sieboldii?

• Project/Area Number: 19710197
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Resource conservation science
• Research Institution: Iwate University (2009-2011); University of Tsukuba (2007-2008)
• Principal Investigator: KITAMOTO Naoko (KITAMOTO Maoko) 岩手大学, 農学部, 特任助教 (70447241)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥5,390,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥1,100,000 (Direct Cost: ¥1,100,000)
• Keywords: 保全 / 近交弱勢 / サクラソウ / 絶滅 / 有害遺伝子 / 連鎖地図 / 保全生物 / QTL

Research Abstract

To reveal the genetic architecture of inbreeding depression, the QTL analysis of inbreeding depression was conducted in a threatened plant, Primula sieboldii. The progeny which have the allele derived from a common ancestry around the locus ga0343, showed high probability of being albino, small leaf, late budding and small number of flowers. This result suggested that there is a deleterious recessive allele around the locus ga0343

Research Products

• [Journal Article] A model to predict the frequency of integration of fitness-related QTLs from cultivated to wild soybean (2012)
  • Author(s): Kitamoto N., A.Kaga, Y.Kuroda, R.Ohsawa
  • Journal Title: Transgenic Research Volume: 21 Pages: 131-138
  • Peer Reviewed
• [Journal Article] QTL analysis of heterostyly in Primula sieboldii and its application for morph identification in wild populations (2011)
  • Author(s): Yoshida, Y., S. Ueno, M. Honjo, N. Kitamoto, M. Nagai, I. Washitani, Y. Tsumura, Y. Yasui, R. Ohsawa
  • Journal Title: Annals of Botany Volume: 108 Pages: 133-142
  • NAID: 120007137307
  • Peer Reviewed
• [Journal Article] Generation of expressed sequence tags, development of microsatellite and single nucleotide polymorphism markers in Primula sieboldii E.Morren (Primulaceae) for analysis of genetic diversity in natural and horticultural populations (2011)
  • Author(s): Ueno, S., Y.Yoshida, Y.Teguchi, T.Ujino-Ihara, N.Kitamoto, M.Honjo, R.Ohsawa, I.Washitani, Y.Tsumura
  • Journal Title: Breeding Science Volume: 61 Pages: 234-243
  • Peer Reviewed
• [Journal Article] Reconsideration for conservation units of wild Primula sieboldii in Japan based on adaptive diversity and molecular genetic diversity (2009)
  • Author(s): Yoshida, Y., M. Honjo, N. Kitamoto and R. Ohsawa
  • Journal Title: Genetics Research Volume: 91 Pages: 225-235
  • NAID: 120007131477
  • Peer Reviewed
• [Journal Article] Management units of the endangered herb Primula sieboldii based on microsatellite variation among and within populations throughout Japan (2009)
  • Author(s): Honjo, M., N. Kitamoto, S. Ueno, Y. Tsumura, I. Washitani, R. Ohsawa
  • Journal Title: Conservation Genetics Volume: 10 Pages: 257-267
  • Peer Reviewed
• [Journal Article] Development of 120 microsatellite markers for Primula sieboldii E. Morren for linkage mapping (2009)
  • Author(s): Ueno, S., Y. Yoshida, Y. Taguchi, M. Honjo, N. Kitamoto, I. Washitani, R. Ohsawa, Y. Tsumura
  • Journal Title: Conservation Genetics 10 Pages: 1945-1952
  • Peer Reviewed
• [Journal Article] Reconsideration for conservation units of wild Primula sieboldii in Japan based on adaptive diversity and molecular genetic diversity (2009)
  • Author(s): Yoshida, Y., M. Honjo, N. Kitamoto, R. Ohsawa
  • Journal Title: Genetics Research 91 Pages: 225-235
  • NAID: 120007131477
  • Peer Reviewed
• [Journal Article] Management units of the endangered herb Primula sieboldii based on microsatellite variation among and within populations throughout Japan (2009)
  • Author(s): Honjo, M., N. Kitamoto, S. Ueno, Y. Tsumura, I. Washitani, R. Ohsawa
  • Journal Title: Conservation Genetics 10 Pages: 257-267
  • Peer Reviewed
• [Journal Article] Management units of the endangered herb Primula sieboldii based on microsatelli to variation among and within populations throughout Japan (2009)
  • Author(s): Honjo, M., N.Kitamoto, S.Ueno, Y.Tsumura, I.Washitani, R.Ohsawa
  • Journal Title: Conservation Genetics 10 Pages: 257-267
  • Peer Reviewed
• [Journal Article] Reconsideration for conservation units of wild Primula sieboldii in Japan based on adaptive diversity and molecular genetic diversity (2009)
  • Author(s): Yoshida, Y., M.Honjo, N.Kitamoto, R.Ohsawa
  • Journal Title: Genetics Research 91 Pages: 225-235
  • NAID: 120007131477
  • Peer Reviewed
• [Journal Article] Development of 120 microsatellite markers for Primula sieboldii E.Morren for linkage mapping (2009)
  • Author(s): Ueno, S., Y.Yoshida, Y.Taguchi, M.Honjo, N.Kitamoto, I.Washitani, R.Ohsawa, Y.Tsumura
  • Journal Title: Conservation Genetics 10 Pages: 1945-1952
  • Peer Reviewed
• [Journal Article] Effect of population density of compatible neighbours on inbreeding level within a Primula sieboldii population (2008)
  • Author(s): Kitamoto, N., S. Ueno, Y. Tsumura, I. Washitani and R. Ohsawa
  • Journal Title: Ecological Research Volume: 23 Pages: 307-315
  • Peer Reviewed
• [Journal Article] Effect of population density of compatible neighbours on inbreeding level within a Primula sieboldii population (2008)
  • Author(s): Kitamoto, N., S. Ueno, Y. Tsumura, I. Washitani, R. Ohsawa
  • Journal Title: Ecological Research 23 Pages: 307-315
  • Peer Reviewed
• [Presentation] サクラソウの異型花柱性に関するQTL解析 (2009)
  • Author(s): 吉田康子, 上野真義, 本城正憲, 北本尚子, 永井美穂子, 鷲谷いづみ, 津村義彦, 大澤良
  • Organizer: 第116回日本育種学会
  • Place of Presentation: 札幌
  • Year and Date: 2009-09-26
• [Presentation] 鷲谷いつみ,大澤良(2009)サクラソウの受粉から結実までに働く近交弱勢の関与遺伝子座数とその効果の解析 (2009)
  • Author(s): 北本尚子,本城正憲,吉田康子,上野真義,津村義彦
  • Organizer: 第56回日本生態学会
  • Place of Presentation: 岩手県立大学(盛岡)
  • Year and Date: 2009-03-19
• [Presentation] 鷲谷いつみ,津村義彦,大澤良(2009)適応に関連したQTLに基づく野生サクラソウ集団の遺伝的多様性評価 (2009)
  • Author(s): 吉田康子,上野真義,北本尚子,小玉昌孝,本城正憲,田口由利子,永井美穂子
  • Organizer: 第56回日本生態学会
  • Place of Presentation: 岩手県立大学(盛岡)
  • Year and Date: 2009-03-19
• [Presentation] サクラソウの受粉から結実までに働く近交弱勢の関与遺伝子座数とその効果の解析 (2009)
  • Author(s): 北本尚子, 本城正憲, 吉田康子, 上野真義, 津村義彦, 鷲谷いづみ, 大澤良
  • Organizer: 第56回日本生態学会
  • Place of Presentation: 盛岡
  • Year and Date: 2009-03-19
• [Presentation] 適応に関連したQTLに基づく野生サクラソウ集団の遺伝的多様性評価 (2009)
  • Author(s): 吉田康子, 上野真義, 北本尚子, 小玉昌孝, 本城正憲, 田口由利子, 永井美穂子, 鷲谷いづみ, 津村義彦, 大澤良
  • Organizer: 第56回日本生態学会
  • Place of Presentation: 盛岡
  • Year and Date: 2009-03-19
• [Presentation] サクラソウの受粉から結実までに働く近交弱勢の関与遺伝子座数とその効果の解析 (2009)
  • Author(s): 北本尚子
  • Organizer: 第56回日本生態学会
  • Place of Presentation: 岩手県立大学
  • Year and Date: 2009-03-19
• [Book] 第3章植物の保全遺伝学的解析・評価法(「保全生態学の手法～調査・研究・実践マニユアル」鷲谷いつみ,宮下直,西廣淳,角谷拓編) (2010)
  • Author(s): 本城正憲,北本尚子
  • Publisher: 東京大学出版会,東京
• [Book] 保全生態学の手法～調査・研究・実践マニュアル(鷲谷いづみ, 宮下直, 西廣淳, 角谷拓編) (2010)
  • Author(s): 本城正憲, 北本尚子
  • Publisher: 東京大学出版会
• [Book] 保全生態学の手法～調査・研究・実践マニュアル 第3章 植物の保全遺伝学的解析・評価法 (2010)
  • Author(s): 本城正憲・北本尚子
  • Total Pages: 323
  • Publisher: 東京大学出版会