| Project/Area Number |
19740315
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Geology
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
TAKEUCHI Mio National Institute of Advanced Industrial Science and Technology, 地圏資源環境研究部門, 主任研究員 (20357403)
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| Project Period (FY) |
2007 – 2009
|
| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥3,270,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥570,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | 細胞外DNA / 地下圏微生物 / RNA / 古細菌 / 微生物相 / 陸域地下圏 / 微生物相解析 / 嫌気的メタン酸化 / 粘土層 / 炭素循環 / 地球化学 / 大深度地下 / 核酸 / 微生物 / 堆積物 |
|---|
| Research Abstract |
Extracellular DNA was quantified with terrestrial subsurface sediments. Extracellular DNA was detected at 2.9-28.3 ng/g dry weight sediment and decreased with depth. Microbial community analysis using the extracellular DNA yielded significantly low number of OTUs, suggesting that the extracellular DNA was fragmented or damaged in the environment. RNA based community analysis revealed variety of active microbes (Proteobacteria, Chloroflexi, Acidobacteria, Miscellaneous Crenarchaeotic Group (MCG), ANME) in the terrestrial subsurface environment.
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