Hypothesis of the second critical point of water and protein folding mechanism

• Project/Area Number: 19750010
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Physical chemistry
• Research Institution: Fukuoka University
• Principal Investigator: YOSHIDA Koji Fukuoka University, 理学部, 助教 (00309890)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,020,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥720,000); Fiscal Year 2009: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2007: ¥900,000 (Direct Cost: ¥900,000)
• Keywords: 生物物理 / 中性子散乱 / タンパク質 / 水和 / 化学物理 / 液体

Research Abstract

Dynamics of hydration water around proteins and confined water in nano-porous materials (silica and dextran gel) was measured with X-ray and neutron scattering experiments. The temperature behaviors in the relaxation time of confined water molecules in the porous silica change from the Ahrrenus type to the non-Ahrrenus one at around 220 K (fragile-strong transition) where the atomic motion of hydrated proteins changes from a harmonic to anharmonic behavior. From these results, the role of water in protein folding was discussed.

Research Products

• [Journal Article] A Study of alcohol-induced gelation of beta-lactoglobulin with small-angle neutron scattering, neutron spin echo, and dynamic light scattering measurements (2010)
  • Author(s): K. Yoshida, T. Yamaguchi, N. Osaka, H. Endo, M. Shibayama
  • Journal Title: Phys. Chem. Chem. Phys. 12 Pages: 3260-3269
  • Peer Reviewed
• [Journal Article] Ion hydration in aqueous solutions of lithium chloride, nickel chloride, and cesium chloride in ambient to supercritical water (2010)
  • Author(s): T. Yamaguchi, H. Ohzono, M. Yamagami, K. Yamanaka, K. Yoshida, H. Wakita
  • Journal Title: Journal of Molecular Liquids 201(in press)
  • Peer Reviewed
• [Journal Article] A Study of alcohol-induced gelation of beta-lactoglobulin with small-angle neutron scattering, neutron spin echo, and dynamic light scattering measurements (2010)
  • Author(s): K.Yoshida, T.Yamaguchi, N.Osaka, H.Endo, M.Shibayama
  • Journal Title: Phys.Chem.Chem.Phys. 12 Pages: 3260-3269
  • Peer Reviewed
• [Journal Article] Ion hydration in aqueous solutions of lithium chloride, nickel chloride, and cesium chloride in ambient to supercritical water (2010)
  • Author(s): T.Yamaguchi, H.Ohzono, M.Yamagami, K.Yamanaka, K.Yoshida, H.Wakita
  • Journal Title: Journal of Molecular Liquids (In press)
  • Peer Reviewed
• [Journal Article] Thermodynamic, structural and dynamic properties of supercooled water confined in mesoporous MCM-41 studied with calorimetric, neutron diffraction, and neutron spin echo measurements (2009)
  • Author(s): K. Yoshida, T. Yamaguchi, S. Kittaka, M. -C. Bellissent-Funel, P. Fouquet
  • Journal Title: J. Chem. Phys. 129
  • Peer Reviewed
• [Journal Article] On the solvent role in alcohol-induced a-helix formation of chymotrypsin inhibitor-2 (2009)
  • Author(s): K. Yoshida, J. Kawaguchi, S. Lee, T. Yamaguchi
  • Journal Title: Pure Appl. Chem. 80 Pages: 1337-1347
  • Peer Reviewed
• [Journal Article] 中性子スピンエコー法による過冷却状態における細孔水の動的挙動 (2008)
  • Author(s): 吉田亨次
  • Journal Title: 福岡大学理学集報 39 Pages: 55-62
  • NAID: 110007039878
  • Peer Reviewed
• [Journal Article] Thermodynamic, structural and dynamic properties of supercooled water confined in mesoporous MCM-41 studied with calorimetric, neutron diffraction, and neutron spin echo measurements (2008)
  • Author(s): K. Yoshida, T. Yamaguchi, S. Kittaka, M.-C. Bellissent-Funel, P. Fouquet
  • Journal Title: J. Chem. Phys 129
  • Peer Reviewed
• [Journal Article] On the solvent role in alcohol-induced α-helix formation ofchymotrypsin inhibitor-2 (2008)
  • Author(s): K. Yoshida, J. Kawaguchi, S. Lee, T. Yamaguchi
  • Journal Title: Pure Appl. Chem 80 Pages: 1337-1347
  • Peer Reviewed
• [Journal Article] On the solvent role in alcohol-induced α-helix formation of chymotrypsin inhibitor-2 (2008)
  • Author(s): Koji Yoshida, Junko Kawaguchi, Sannum Lee, Toshio Yamaguchi
  • Journal Title: Pure and Applied Chemistry (In press)
  • Peer Reviewed
• [Journal Article] Hydration water in dynamics of a hydrated P-lactoglobulin (2007)
  • Author(s): K. Yoshida, T. Yamaguchi, M. -C. Belllissent-funel, S. Longeville
  • Journal Title: Eur. Phys. J. Special Topics 141 Pages: 223-226
  • Peer Reviewed
• [Journal Article] Structure and dynamic properties of liquids confined in MCM-41 mesopores (2007)
  • Author(s): T. Yamaguchi, K. Yoshida, P. Smirnov, T. Takamuku, S. Kittaka, S. Takahara, Y. Kuroda, M. -C. Bellissent-Funel
  • Journal Title: Eur. Phys. J. Special Topics 141 Pages: 19-27
  • Peer Reviewed
• [Journal Article] Hydration water in dynamics of a hydrated β-lactoglobulin (2007)
  • Author(s): K. Yoshida, T. Yamaguchi, M.-C. Belllissent-funel, S. Longeville
  • Journal Title: Eur. Phys. J. Special Topics 141 Pages: 223-226
  • Peer Reviewed
• [Journal Article] Structure and dynamic properties of liquids confined in MCM-41 mesopores (2007)
  • Author(s): T. Yamaguchi, K. Yoshida, P. Smimov, T. Takamuku, S. Kittaka, S. Takahara, Y. Kuroda, M.-C. Bellissent-Funel
  • Journal Title: Eur. Phys. J. Special Topics 141 Pages: 19-27
  • Peer Reviewed
• [Presentation] 中性子スピンエコー法による多孔性シリカ中の低温水のダイナミクス (2009)
  • Author(s): 吉田亨次, 山口, 橘高, M.C. Bellissent-Funel, P. Fouquet
  • Organizer: 第32回溶液化学シンポジウム
  • Place of Presentation: 新潟市
  • Year and Date: 2009-11-18
• [Presentation] 中性子スピンエコー法による多孔性シリカ中の低温水のダイナミクス (2009)
  • Author(s): 吉田, 山口, 橘高, M.C.Bellissent-Funel, P.Fouquet
  • Organizer: 第32回溶液化学シンポジウム
  • Place of Presentation: 新潟大学
  • Year and Date: 2009-11-18
• [Presentation] Collective Dynamics of Hydrated Protein by Inelastic X-ray Scattering (2009)
  • Author(s): K. Yoshida, T. Yamaguchi, S. Hosokawa, A.Q.R. Baron
  • Organizer: European Molecular Liquid Group Meeting 2009
  • Place of Presentation: Salzburg (Austria)
  • Year and Date: 2009-09-08
• [Presentation] Collective Dynamics of Hydrated Protein by Inelastic X-ray Scattering (2009)
  • Author(s): K.Yoshida, T.Yamaguchi, S.Hosokawa, A.Q.R.Baron
  • Organizer: European Molecular Liquid Group Meeting 2009
  • Place of Presentation: Salzburg(オーストリア)
  • Year and Date: 2009-09-08
• [Presentation] 中性子散乱および動的光散乱によるβ-ラクトグロブリンの構造と運動に対するアルコールの影響 (2008)
  • Author(s): 吉田亨次・山口敏男・遠藤仁・柴山充弘
  • Organizer: 第2回分子科学討論会
  • Place of Presentation: 福岡市
  • Year and Date: 2008-09-24
• [Presentation] 二次元NMRおよびレプリカ交換MDによるモデルペプチドのアルコール変性機構の研究 (2007)
  • Author(s): 吉田亨次、KRISHTAL、藤永、李、根本、金澤、山口
  • Organizer: 第1回分子科学討論会
  • Place of Presentation: 仙台市
  • Year and Date: 2007-09-19
• [Presentation] 二次元NMRおよびレプリカ交換MDによるモデルペプチドのアルコール変性機構の研究 (2007)
  • Author(s): 吉田、KRISHTAL、藤永、李、根本、金渾、山口
  • Organizer: 第1回分子科学討論会
  • Place of Presentation: 東北大学
  • Year and Date: 2007-09-19
• [Presentation] 中性子散乱法によるナノ細孔中の水の構造とダイナミクス" (2007)
  • Author(s): 吉田亨次
  • Organizer: 第68回分析化学討論会若手企画シンポジウム
  • Place of Presentation: 宇都宮市
  • Year and Date: 2007-05-20
• [Presentation] 中性子散乱法によるナノ細孔中の水の構造とダイナミクス (2007)
  • Author(s): 吉田亨次
  • Organizer: 第68回分析化学討論会若手企画シンポジウム
  • Place of Presentation: 宇都宮大学
  • Year and Date: 2007-05-20