Development of novel single-molecule vibrational spectroscopy toward uncovering slow dynamics of complex systems

• Project/Area Number: 19750019
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Physical chemistry
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: ISHII Kunihiko The Institute of Physical and Chemical Research, 田原分子分光研究室, 研究員 (80391853)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,790,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,100,000 (Direct Cost: ¥2,100,000)
• Keywords: 単一分子分光 / 蛍光相関分光 / 蛍光寿命

Research Abstract

生体高分子など複雑な分子の遅いダイナミクスを解明するための手段として、複雑分子の構造変化の鋭敏なプローブとなる蛍光寿命に着目し、その揺らぎを単一分子レベルで測定する蛍光寿命相関分光法を開発した。これを応用して、複数の分子種を含む試料の不均一性を、蛍光寿命を鍵として検知することができた。この方法は今後、複雑分子の構造の多様性や揺らぎの時間スケールの観測の新しい手段になると期待される。

Research Products

• [Journal Article] Pronounced Non-Condon Effect as the Origin of the Quantum Beat Observed in the Time-Resolved Absorption Signal from Excited-State cis-Stilbene (2008)
  • Author(s): Kunihiko Ishii, Satoshi Takeuchi, and Tahei Tahara
  • Journal Title: The Journal of Physical Chemistry A 112 Pages: 2219-2227
  • Peer Reviewed
• [Journal Article] Pronounced Non-Condon Effect as the Origin of the Quantum Beat Observed in the Time-Resolved Absorption Signal from Excited-State cis-Stilbene
  • Author(s): 石井 邦彦
  • Journal Title: The Journal of Physical Chemistry A (印刷中)
  • Peer Reviewed
• [Presentation] 蛍光寿命相関分光法による溶液中分子の揺らぎ検出の試み (2009)
  • Author(s): 石井邦彦
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 船橋
  • Year and Date: 2009-03-28
• [Presentation] カルシウムセンサー蛍光タンパク質cameleonの共鳴エネルギー移動効率の不均一性 (2008)
  • Author(s): 石井邦彦
  • Organizer: 第2回分子科学討論会2008
  • Place of Presentation: 福岡
  • Year and Date: 2008-09-26
• [Presentation] Mid-IR-induced nuclear wavepacket motion of a hydrogen bonding system : effects of mechanical and electrical anharmonic couplings (2008)
  • Author(s): 石井邦彦
  • Organizer: 16th International Conference on Ultrafast Phenomena
  • Place of Presentation: イタリア
  • Year and Date: 2008-06-09
• [Presentation] Mid-IR-induced nuclear wavepacket motion of a hydrogen bonding system; effects of mechanical and electrical anharmonic couplings (2008)
  • Author(s): 石井邦彦
  • Organizer: 16th International Conference on Ultra fast Phenomena
  • Place of Presentation: ストレーザ、イタリア
  • Year and Date: 2008-06-09
• [Presentation] Anharmonic Coupling in a Hydrogen Bonding System Revealed by Impulsive Mid-IR Excitation (2008)
  • Author(s): 石井邦彦
  • Organizer: 5th Asian Conference on Ultrafast Phenomena
  • Place of Presentation: シンガポール
  • Year and Date: 2008-01-06
• [Presentation] 中赤外極短パルスを用いた分子内非調和振動結合の研究 (2007)
  • Author(s): 石井邦彦
  • Organizer: 第6回エクストリーム・フォトニクス研究会
  • Place of Presentation: 蒲郡
  • Year and Date: 2007-11-14
• [Presentation] Site-specific Picosecond Dynamics in Single Lipid Vesicles Studied by Fluorescence Microscopy (2007)
  • Author(s): Sobhan Sen
  • Organizer: 平成19年度日本分光学会顕微分光部会シンポジウム「分子イメージングに向けた分光技術の展開」
  • Place of Presentation: 東京
  • Year and Date: 2007-10-26
• [Presentation] Site-specific Picosecond Dynamics in Single Lipid Vesicle Studied by Fluorescence Microscopy (2007)
  • Author(s): Sobhan Sen
  • Organizer: 平成19年度日本分光学会顕微分光部会シンポジウム「分子イメージングに向けた分光技術の展開」
  • Place of Presentation: 東京
  • Year and Date: 2007-10-26
• [Presentation] 単一脂質ベシクル中の位置特異的な超高速ダイナミクス : ピコ秒時間分解顕微鏡による研究 (2007)
  • Author(s): Sobhan Sen
  • Organizer: 分子科学討論会2007
  • Place of Presentation: 仙台
  • Year and Date: 2007-09-20
• [Presentation] 単一脂質ベシクル中の位置特異的な超高速ダイナミクス:ピコ秒時間分解蛍光顕微鏡による研究 (2007)
  • Author(s): Sobhan Sen
  • Organizer: 分子科学討論会2007
  • Place of Presentation: 仙台
  • Year and Date: 2007-09-20