| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥600,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Research Abstract |
In the biotinylation reaction, biotin protein ligase (BPL) mediates the attachment of biotin to a specific lysine residue of biotin carboxyl carrier protein (BCCP). The biotinylation reaction from thermophilic archaeon Sulfolobus tokodaii has a unique characteristic that the enzyme BPL forms a stable complex with its product, biotinylated BCCP. In the present work, we have developed a detection system for ATP with this unique biotinylation reaction.
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